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No products found
because this supplier's products are not listed.
Ian A.P. Thompson, et al.,
bioRxiv - Bioengineering 2023
Quote:
Standard automated oligonucleotide solid-phase synthesis was performed on an Expedite 8909 synthesizer from Biolytic Lab Performance. DNA was quantified by UV absorbance at 260 nm with the NanoDrop 2000 spectrophotometer from Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
Swayam Prakash, et al.,
bioRxiv - Immunology 2020
Quote:
... and ORF10 were synthesized using solid-phase peptide synthesis and standard 9-fluorenylmethoxycarbonyl technology (21st Century Biochemicals, Inc). The purity of peptides was over 90% ...
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No products found
because this supplier's products are not listed.
Celestia Fang, et al.,
bioRxiv - Genomics 2020
Quote:
... and size selected for fragments approximately 100–250 base pairs in length using solid phase reversible immobilization (SPRI) beads (MagBio Genomics). Resulting DNA underwent bisulfite conversion using the EZ DNA Methylation-Lightning Kit (Zymo Research) ...
-
No products found
because this supplier's products are not listed.
Thomas Lund Koch, et al.,
bioRxiv - Evolutionary Biology 2021
Quote:
... teleta was synthesized by solid-phase peptide synthesis (SPPS) in 0.1 mmol scale using preloaded Fmoc-Trp Tentagel R HMPA resin from Rapp Polymere (Tuebingen, Germany). The sequence was H-ZASSGAQICIWKVCPPTPW-OH ...
-
No products found
because this supplier's products are not listed.
Yi Huang, et al.,
bioRxiv - Bioengineering 2023
Quote:
The (KKEEE)3K targeting peptide was synthesized using standard Fmoc-mediated solid phase peptide synthesis methods on rink Amide resin (Anaspec, Fremont, CA, USA) using an automated benchtop peptide synthesizer (PS3 ...
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No products found
because this supplier's products are not listed.
Christopher Cyrus Kuhn, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Bound integrins were quantified by an enzyme-linked immunosorbent (ELISA)-like solid-phase assay using biotinylated rabbit anti-velcro (against ACIS/BASE coiled-coil) antibody and HRP-conjugated streptavidin (VECTOR Laboratories #SA-5004). After addition of ABTS ...
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No products found
because this supplier's products are not listed.
Lysbeth H. Antonides, et al.,
bioRxiv - Biophysics 2022
Quote:
... 1.2 g of FC16 solid (Cube Biotech (Cat #16038) was added to the suspension at a final concentration of 1.2 % (w/v) ...
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No products found
because this supplier's products are not listed.
Claudine Bleykasten-Grosshans, et al.,
bioRxiv - Genomics 2021
Quote:
... The solid selective media YPD-hygro was supplemented with hygromycin (Euromedex) to a final concentration of 200 μg/ml and with 2% agar (Bacto agar Difco) ...
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No products found
because this supplier's products are not listed.
Zixi Wang, et al.,
bioRxiv - Cell Biology 2023
Quote:
... It is composed of high fat solid food (ENVIGO #TD.120528) and high sugar water containing 23.1g/L d-fructose (Sigma-Aldrich #F0127 ...
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No products found
because this supplier's products are not listed.
Vinciane Saint-Criq, et al.,
bioRxiv - Physiology 2021
Quote:
... Polystyrene black dyed microspheres (diameter 6μm, 2.6% solid-latex, Polybead, Polyscience Inc) were washed and resuspended in HCO3- or HEPES solution and 4 μl of particle solution with 0.3% latex were added onto the mucosal surface of the trachea ...
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No products found
because this supplier's products are not listed.
Agnes Adler, et al.,
bioRxiv - Biophysics 2023
Quote:
For the preparation of solid-state NMR samples lyophilized tubulin (Cytoskeleton, Inc.) was solubilised in BRB80 buffer (80 mM PIPES ...
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No products found
because this supplier's products are not listed.
Ginaini Grazielli Doin de Moura, et al.,
bioRxiv - Evolutionary Biology 2022
Quote:
... diluted and spread on selective solid medium using an easySpiral automatic plater (Interscience). After 2-day incubation at 28°C ...
-
No products found
because this supplier's products are not listed.
Thomas V. Sydenham, et al.,
bioRxiv - Microbiology 2019
Quote:
... and cultured on solid chocolate agar with added vitamin K and cysteine (SSI Diagnostica) for 48 hrs in an anaerobic atmosphere at 35 °C ...
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No products found
because this supplier's products are not listed.
Erik L Hendrickson, et al.,
bioRxiv - Microbiology 2022
Quote:
... Volta Phase Plate (VPP) and energy filter (Gatan). SerialEM (27 ...
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No products found
because this supplier's products are not listed.
Ansuman Sahoo, et al.,
bioRxiv - Molecular Biology 2022
Quote:
Yeast strains were cultivated in liquid or on solid (2% agar) YPD media (Teknova, USA) or Synthetic Complete (SC ...
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No products found
because this supplier's products are not listed.
Renato M. Rossi, et al.,
bioRxiv - Immunology 2022
Quote:
... we determined the concentration of the positive acute phase protein Haptoglobin (Hp; Phase Haptoglobin Assay TP-801, Tridelta Development Limited, Maynooth, Ireland). All biomarkers related to nutrient utilization and inflammation were determined using a small-scale biochemistry analyzer (CataChemWell-T ...
-
No products found
because this supplier's products are not listed.
Eftychios Frangedakis, et al.,
bioRxiv - Plant Biology 2021
Quote:
... and moved onto solid KNOP medium plates supplemented with 100 μg/mL cefotaxime (#BIC0111, Apollo Scientific) and 10 μg/mL Hygromycin (#10687010 ...
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No products found
because this supplier's products are not listed.
Veedaa Soltaniband, et al.,
bioRxiv - Plant Biology 2023
Quote:
... solid medium [15 g L−1 of CRITERION™ Agar (Hardy Diagnostics, Santa Maria CA, USA)] ...
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No products found
because this supplier's products are not listed.
Mustafa Sibai, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... UPX protein extraction buffer (Expedeon) was used for protein extraction as per the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Chieh Chen, et al.,
bioRxiv - Physiology 2022
Quote:
... N2 worms were egg-prepped onto solid NGM with 50 μg/ml carbenicillin (GoldBio, C-103-5) and HT115(DE3 ...
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No products found
because this supplier's products are not listed.
Lior Tal, et al.,
bioRxiv - Plant Biology 2023
Quote:
... Germination was assessed on solid medium supplied with ½ Murashige Skoog salt mixture without MES (Caisson labs MSP01). Harvested seeds were collected immediately and stored at −80°C to preserve primary dormancy ...
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No products found
because this supplier's products are not listed.
Roberta Cacioppo, et al.,
bioRxiv - Cell Biology 2023
Quote:
... for G2 phase by incubating with 10μM RO3306 (4181, Tocris Bioscience) for 16h ...
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No products found
because this supplier's products are not listed.
Justin Judd, Jonathan Lovas, Guo N. Huang,
bioRxiv - Developmental Biology 2019
Quote:
S-phase activity was detected by addition of EdU (Santa Cruz Biotechnology) to cell culture media between days 2-7 post-transduction ...
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No products found
because this supplier's products are not listed.
Noy Sadot Muzika, et al.,
bioRxiv - Plant Biology 2024
Quote:
Cubes (1×1×0.5cm) were cut from a petri dish with 25ml solid media using a cuvette (Sarstedt No.67.755). The cubes were then carefully weighted.
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No products found
because this supplier's products are not listed.
José Gustavo Ramírez-Paredez, et al.,
bioRxiv - Microbiology 2020
Quote:
... For this the homogenised tissues were subjected to total nucleic acids extraction (~20mg of each organ) using the Genesig Easy DNA/RNA Extraction Kit (Primerdesign) as described earlier ...
-
No products found
because this supplier's products are not listed.
Maxime Assous, et al.,
bioRxiv - Neuroscience 2019
Quote:
The open field apparatus measured 40 cm x 40 cm and was constructed of opaque gray plastic with a solid metal base (Stoelting Co, USA). The apparatus had overhead lighting and the illumination at the center of the base was approximately 400 lumens ...
-
No products found
because this supplier's products are not listed.
Choco Michael Gorospe, et al.,
bioRxiv - Cell Biology 2022
Quote:
... S and G2 phases was performed using FCS Express 7 Flow (De Novo Software). The y-axis scale of the cell cycle histograms was adjusted to fit the highest cell count.
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No products found
because this supplier's products are not listed.
Rishi Kumar Nageshan, et al.,
bioRxiv - Molecular Biology 2023
Quote:
Mid log-phase cells were adhered to 35-mm glass culture dishes (MatTek Corporation) using 0.2 mg/ml of soybean lectin (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Sílvia Vale-Costa, et al.,
bioRxiv - Cell Biology 2023
Quote:
Extraction of RNA from samples in NZYol (NZYtech, MB18501) was achieved by using the Direct-zol RNA minipreps (Zymo Research ...
-
No products found
because this supplier's products are not listed.
Fabian Morales-Polanco, et al.,
bioRxiv - Cell Biology 2020
Quote:
... exponential-phase yeast were fixed in 4% EM-grade formaldehyde (15714-S; Electron Microscopy Sciences) for 45 min ...
-
No products found
because this supplier's products are not listed.
Cheenou Her, et al.,
bioRxiv - Biophysics 2022
Quote:
... Biomiga MV Gel/PCR extraction kits were purchased from Biomiga. Tris-(carboxyethyl ...
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No products found
because this supplier's products are not listed.
Michael D. Crabtree, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Phase separated solutions were then transferred to a 0.22 mm thick siliconized glass coverslip (Hampton Research), before sealing with 0.12 mm imaging spacers (Sigma ...
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No products found
because this supplier's products are not listed.
Sujoy Chatterjee, et al.,
bioRxiv - Immunology 2020
Quote:
The nuclear extracts were prepared by nuclear extraction kits (Signosis Inc). Gel shift assays were done with EMSA kits (Signosis Inc) ...
-
No products found
because this supplier's products are not listed.
Sophie Stephenson, et al.,
bioRxiv - Immunology 2021
Quote:
... proteins were extracted using a cytoplasmic and nuclear extraction kit (Boster Bio) and protein concentration determined by BCA assay (Boster Bio) ...
-
No products found
because this supplier's products are not listed.
Xiaoli Li, et al.,
bioRxiv - Molecular Biology 2019
Quote:
Total RNAs extraction was performed using a commercial TRIzon Reagent (CoWin Biosciences) and the manufacturer’s instructions with slight modifications ...
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No products found
because this supplier's products are not listed.
Dina J. Ashour, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... The aqueous phase was carefully collected and added to equal amounts of GFP-nanobody coated magnetic beads (Chromotek), equilibrated in lysis buffer ...
-
No products found
because this supplier's products are not listed.
Sascha A.L. Mueller, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and a mobile phase composed of MeCN/H2O/Et3N (40/60/0.1%) (Decon Labs, ThermoFisher Scientific, Waltham, MA). The peak corresponding to [11C]DCZ was collected and diluted in 60 mL water and trapped on a tC18 light cartridge (Waters ...
-
No products found
because this supplier's products are not listed.
Anuja Tripathi, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... The phase composition of the Cu coated nanotextured steel was determined by X-ray powder diffractometry (Rigaku XRD). The diffraction patterns of samples were recorded in the range of 2□ = 20-80° using a Cu radiation source with fixed power (40 kV ...
-
No products found
because this supplier's products are not listed.
Soham Dibyachintan, et al.,
bioRxiv - Evolutionary Biology 2024
Quote:
... the plasmid DNA was extracted from bacteria using a plasmid extraction kit (FroggaBio) to validate the plasmid single mutant libraries ...
-
No products found
because this supplier's products are not listed.
Chidiebere Akusobi, et al.,
bioRxiv - Microbiology 2022
Quote:
... and Mtb were grown to mid-log phase and diluted to an OD600 = 0.003 in each well of non-treated 96-well plates (Genesee Scientific) containing 100 μL of antibiotic serially diluted in 7H9 + OADC + 5 μg/mL clavulanate (Sigma Aldrich) ...
-
No products found
because this supplier's products are not listed.
Zaid Abdo, et al.,
bioRxiv - Immunology 2019
Quote:
... Pellets for supernatant extraction were weighed and 10 µL of 2x ProteaseArrest (G-Biosciences) was freshly diluted in cold PBS (from 50x stock ...
-
No products found
because this supplier's products are not listed.
Shawn M. Costello, et al.,
bioRxiv - Biophysics 2021
Quote:
... peptides were desalted for 4 minutes on a hand-packed trap column (Thermo Scientific POROS R2 reversed-phase resin 1112906, 1 mm ID x 2 cm, IDEX C-128). Peptides were then separated with a C8 analytical column (Thermo Scientific BioBasic-8 5 μm particle size 0.5 mm ID x 50 mm 72205-050565 ...
-
No products found
because this supplier's products are not listed.
Marius Walter, et al.,
bioRxiv - Microbiology 2022
Quote:
... the supernatant was collected and mixed with two volumes of RNA STAT-60 extraction buffer (AMSbio, UK). Cells were similarly harvested by adding RNA STAT-60 extraction buffer ...
-
No products found
because this supplier's products are not listed.
Serdar Turkarslan, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
... The cultures were kept in stationary-phase for approximately 20 hours in order to ensure similar growth phases before dilution into 96-well plates (Thomas Scientific: 1154Q44) for the density dilution assay ...
-
No products found
because this supplier's products are not listed.
Yu-Chieh David Chen, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... The split-GAL4 donor plasmids with appropriate splicing phases were obtained from Addgene, including pBS-KS-attB2-SA(0)-T2A-GAL4DBD-Hsp70 (Addgene #62902) ...
-
No products found
because this supplier's products are not listed.
Ivan Ge, Gregory W. Kirschen, Xinxing Wang,
bioRxiv - Neuroscience 2021
Quote:
... several recovery standards were used before the first step in the extraction process to allow confirmation of extraction efficiency (MicroLab STAR system, Hamilton Company). To remove protein ...
-
No products found
because this supplier's products are not listed.
Maggie R. Wagner, et al.,
bioRxiv - Plant Biology 2020
Quote:
... We used the Synergy 2.0 Plant DNA Extraction Kit (OPS Diagnostics, Lebanon, NJ, USA) to purify DNA ...
-
No products found
because this supplier's products are not listed.
Marilyn E. Allen, et al.,
bioRxiv - Bioengineering 2021
Quote:
... The continuous phase was homogenized at 6,800 x rpm with the T 25 digital ULTRA-TURRAX® (IKA; Wilmington, NC, USA) and left to stir overnight at 600 x rpm at RT until the organic solvent was evaporated and nanocarriers were hardened ...
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No products found
because this supplier's products are not listed.
Sarah A. Peck Justice, et al.,
bioRxiv - Systems Biology 2019
Quote:
... and then packing the capillary with C18 reverse phase resin (particle size: 3 μm diameter; Dr. Maisch HPLC GmbH, Ammerbuch, Germany). The peptides were eluted using a varying mobile phase (MP ...
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No products found
because this supplier's products are not listed.
Morris Vanegas, et al.,
bioRxiv - Bioengineering 2022
Quote:
... allowing for a plate separation ranging from 300 mm (fully released) to 0 mm (fully closed) using a 2-phase stepper motor (Oriental Motors, Braintree, MA, USA). A linear encoder (ETI Systems ...