-
No products found
because this supplier's products are not listed.
Thomas V. Sydenham, et al.,
bioRxiv - Microbiology 2019
Quote:
... Strains were stored at −80° in beef extract broth with 10% glycerol (SSI Diagnostica) and cultured on solid chocolate agar with added vitamin K and cysteine (SSI Diagnostica ...
-
No products found
because this supplier's products are not listed.
Hui-Hsuan Kuo, et al.,
bioRxiv - Cell Biology 2019
Quote:
... 10 μg ml−1 Oryza sativa-derived recombinant human transferrin (Optiferrin, InVitria, 777TRF029-10G,), 14 ng ml−1 sodium selenite (Sigma ...
-
No products found
because this supplier's products are not listed.
Stefan Bruder, et al.,
bioRxiv - Bioengineering 2019
Quote:
... Cell lysis was performed, using a Vortexer (10 min, 3000 rpm) from Heathrow Scientific (indicated as non-optimized extraction method in Suppl ...
-
No products found
because this supplier's products are not listed.
Thomas Swartjes, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... destained in 0.5xTB buffer for 10 minutes and visualized using the UVITEC Alliance (UVITEC).
-
No products found
because this supplier's products are not listed.
Yiming Li, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... was incubated with a 10-fold molar excess of DY634-NHS (634-01, Dyomics) in a final volume of 100ul PBS pH 7,4 overnight at RT ...
-
No products found
because this supplier's products are not listed.
CP Profaci, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Three hours after CNO injection the mice were live-decapitated using a mouse decapitator (LabScientific, XM-801) and brain endothelial cells were isolated for RNA sequencing.
-
No products found
because this supplier's products are not listed.
Javad Najafi, Serge Dmitrieff, Nicolas Minc,
bioRxiv - Biophysics 2022
Quote:
... a suspension of 10 μl of 1.2 μm hydrophobic superparamagnetic beads (magtivio; MagSi-proteomics C18) was washed in 100 μl of 30 ...
-
No products found
because this supplier's products are not listed.
Linghao Hu, et al.,
bioRxiv - Bioengineering 2022
Quote:
... bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl) ethyl sulfide (BPTES, 10 μm, ASTATECH, A11656) was added to the cells in CM3 1 hour before imaging (35) ...
-
No products found
because this supplier's products are not listed.
Tatsuya Ikuta, et al.,
bioRxiv - Biophysics 2020
Quote:
The purified protein was concentrated to 10 mg /mL and mixed with monoolein (Nu-Chek Prep) in a 2:3 protein to lipid ratio (w/w) ...
-
No products found
because this supplier's products are not listed.
Jayne E. Wiarda, et al.,
bioRxiv - Immunology 2022
Quote:
... mouse α-pig γδTCR-iFluor594 (primary antibody Washington State University PG2032; custom conjugation to iFluor594 performed by Caprico Biotechnologies); mouse α-pig CD4-PerCP-Cy5.5 (BD 561474) ...
-
No products found
because this supplier's products are not listed.
Rachel P. Tillage, et al.,
bioRxiv - Neuroscience 2019
Quote:
... and processed according to the manufacturer’s instructions (Galanin Rat and Mouse ELISA kit, S1208, Peninsula Laboratories, San Carlos, CA). Wells were read at 450 nm ...
-
No products found
because this supplier's products are not listed.
Preetha Shridas, et al.,
bioRxiv - Pathology 2023
Quote:
Plasma SAA (SAA1.1 and SAA2.1 isoforms) concentrations were determined using a mouse SAA ELISA kit (cat no TP 802M, Tridelta Development Ltd). Plasma cholesterol concentrations were measured using enzymatic kits (Wako Chemicals).
-
No products found
because this supplier's products are not listed.
Benjamin Tam, et al.,
bioRxiv - Biochemistry 2019
Quote:
In the initial screening 10 fragment molecules from the Maybridge Ro3 2500 Diversity Fragment Library (Maybridge, Inc.) were pooled ...
-
No products found
because this supplier's products are not listed.
A. Weingarten, et al.,
bioRxiv - Neuroscience 2023
Quote:
... incubated in 0.06% potassium permanganate for 10 minutes followed by 0.002% Fluoro-Jade C (Histo-Chem Inc.) for 10 minutes ...
-
No products found
because this supplier's products are not listed.
Yasuaki Uehara, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Phosphate homeostasis hormones were measured in mouse and human serum using the FGF-23 ELISA Kit (Kainos Laboratories Inc., Tokyo, Japan), the mouse or human PTH 1-84 ELISA Kit (Immutopics ...
-
No products found
because this supplier's products are not listed.
Sara Castagnola, et al.,
bioRxiv - Genomics 2020
Quote:
... was performed before cutting the brains sagittally in six equally thick sections (2 mm) using a mouse brain matrix slicer (CellPoint Scientific) and 5 razorblades ...
-
No products found
because this supplier's products are not listed.
Jared D. Chrispell, Yubin Xiong, Ellen R. Weiss,
bioRxiv - Cell Biology 2022
Quote:
... Rabbit polyclonal antibodies against zebrafish Grk7 (27) and phosphorylated mouse Grk1 (17) were generated by 21st Century Biochemicals (Marlboro, MA, USA). A novel rabbit polyclonal antibody against phosphorylated zebrafish GRK1 was also generated by 21st Century Biochemicals using the peptide ISARG[pS]FDGTAN corresponding to amino acids 16-27 of zebrafish Grk1a ...
-
No products found
because this supplier's products are not listed.
Kiryu K. Yap, et al.,
bioRxiv - Cell Biology 2023
Quote:
Human coagulation factor VIII levels in the mouse plasma were measured using a factor VIII enzyme-linked immunosorbent assay (ELISA) kit (Affinity Biologicals), following manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Nishith M Shrimali, et al.,
bioRxiv - Pathology 2021
Quote:
... and incubated with collagen (10 µg/ml) or ADP (2 µM, both from the Bio/Data Corporation, USA) 10 minutes ...
-
No products found
because this supplier's products are not listed.
Kuohan Li, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 10% glycerol and 5 mM β-mercaptoethanol and lysed by a Panda Plus 2000 homogenizer (GEA Niro Soavi). The lysate was clarified by high-speed centrifugation at 20 000×g at 4°C for 40 min ...
-
No products found
because this supplier's products are not listed.
Raleigh M. Linville, et al.,
bioRxiv - Bioengineering 2022
Quote:
... for 90 minutes or 10 μM melittin with a free carboxy C-terminus (melittin-COO-) (Bio-Synthesis Inc) for 10 minutes ...
-
No products found
because this supplier's products are not listed.
Malene E Lindholm, et al.,
bioRxiv - Genetics 2020
Quote:
Neonatal rat ventricular myocytes (NRVMs) were isolated from newly born rats using the neonatal rat/mouse cardiomyocyte isolation protocol from Cellutron (nc-6031) according to the specifications from the manufacturer ...
-
No products found
because this supplier's products are not listed.
Sébastien Durand, et al.,
bioRxiv - Developmental Biology 2021
Quote:
Cells were centrifugated during 10 min at 377g and fixed in an alcohol based fixative solution Thinprep® (Hologic) during 15 min ...
-
No products found
because this supplier's products are not listed.
Homa Majd, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Full-thickness tissues pieces (∼2 cm2) were fixed overnight (<24 hours) in 10% neutral buffered formalin (Cancer Diagnostics, FX1003). Samples were transferred to 70% ethanol prior to paraffin embedding (Leica ASP6025 ...
-
No products found
because this supplier's products are not listed.
Charlène Iltis, et al.,
bioRxiv - Immunology 2021
Quote:
... membranes were stripped at 4°C in agitation using Antibody stripping buffer 1X for 10 minutes (Gene Bio-Application). Protein bands were quantified using ImageJ software ...
-
No products found
because this supplier's products are not listed.
Hsiao-Yun Chen, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... then embedded/inserted into paraffin recipient block/negative mold (IHC World; 10*17 Quick Ray mold IW-UM01-1). Empty slots were filled with blank paraffin cores ...
-
No products found
because this supplier's products are not listed.
Georgia Chatzinikolaou, et al.,
bioRxiv - Genetics 2020
Quote:
... IF:1:50), TAF-6 (TAF2G7, wb: 1:500) and TAF-10 (6TA-2B11, wb: 1:500) were from ProteoGenix. Streptavidin-HRP (wb ...
-
No products found
because this supplier's products are not listed.
Xiaoke Chi, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Membranes were pelleted by centrifugation at 13000g for 10 min and cytochrome c release was analyzed by immunoblotting using a sheep anti-cytochrome c antibody (Capralogics). Mitochondria from embryonic mouse brains for BH3 profiling experiments were prepared from ~20 mouse embryos ...
-
No products found
because this supplier's products are not listed.
Jaewan Jang, et al.,
bioRxiv - Synthetic Biology 2019
Quote:
... A single colony was picked and used to inoculate media containing 10 μM PCB or 40 μM BV (Frontier Scientific). Cultures were grown under different illumination conditions (green ...
-
No products found
because this supplier's products are not listed.
F. Esra Demircioglu, et al.,
bioRxiv - Biochemistry 2019
Quote:
... The lysate was immediately mixed with 0.1 M phenylmethanesulfonyl fluoride (PMSF) (50 μl per 10 ml lysate) and 250 units of TurboNuclease (Eton Bioscience), and cleared by centrifugation ...
-
No products found
because this supplier's products are not listed.
Elena Sánchez Martín-Fontecha, et al.,
bioRxiv - Plant Biology 2024
Quote:
Seven-dpg seedlings grown horizontally in MS medium were pre-treated with the proteasome inhibitors 50 μM MG132 and 20 nM epoxomicin for 1 h and then with 10 μM GR24rac (Strigolab) for further 3 h at 22°C ...
-
No products found
because this supplier's products are not listed.
Andrea M. Chambers, et al.,
bioRxiv - Immunology 2021
Quote:
... Mice were injected with 10 µL/g and 150mg Luciferin/kg body weight with a stock solution of 15 mg/mL D-Luciferin (Syd Labs). The D-Luciferin was injected intra-peritoneally (i.p. ...
-
No products found
because this supplier's products are not listed.
Anna Vilalta, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Proteins in storage buffer were concentrated to between 5-10 mg/ml for use and tested for endotoxin with the EndoZyme recombinant Factor C assay (Hyglos GmbH). Endotoxin levels in all assays were maintained at < 0.1 EU/ml.
-
No products found
because this supplier's products are not listed.
Run Xie, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (PS) in a humidified incubator (IP610, Yamato Scientific) with 5% CO2 and 100% humidity at 37 °C ...
-
No products found
because this supplier's products are not listed.
V. De Maesschalck, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... three explants were taken for each time point and homogenized using a sterile homogenizer tube containing 1.5 mL PBS (Precellys Evolution, 7200 rpm, 2×45” with 10” interval time; Bertin Corp., USA). Next ...
-
No products found
because this supplier's products are not listed.
Laura Martinez-Ruiz, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Tumor pieces were digested using 5–10 mL of a 2.5 mg/mL Collagenase NB4 standard (S1745401 Nordmark Biochemicals, Uetersen, Germany) solution in PBS + 3 mM CaCl2 for 2 hours at 37°C ...
-
No products found
because this supplier's products are not listed.
Olivier Messina, et al.,
bioRxiv - Genomics 2022
Quote:
... attached to a 1:10 poly(L-lysine):water coated coverslip and mounted into a FCS2® flow chamber (Bioptechs, USA). ~20-30 embryos were selected and imaged using two regions of interest (ROI 200×200μm2) ...
-
No products found
because this supplier's products are not listed.
Clara M. Herrera, et al.,
bioRxiv - Microbiology 2024
Quote:
... Digested peptide samples were acidified to 0.5% TFA (ph<2) with 10% TFA stock and desalted using C18 ultra micro spin columns (The Nest Group).
-
No products found
because this supplier's products are not listed.
Erica L. Stone, et al.,
bioRxiv - Immunology 2021
Quote:
... blocks were cut in 5 μm sections that were placed on glass slides for anti-IgG (UltraPolymer Goat anti-Mouse heavy and light chain IgG-HRP, Cell IDx, San Diego, CA, USA) or anti-C3 (EPR19394 ...
-
No products found
because this supplier's products are not listed.
Ophir Vermesh, et al.,
bioRxiv - Bioengineering 2021
Quote:
... in ethanol were prepared in Eppendorf tubes and spiked into 10 mL of media (DMEM with 10% FBS) to final concentrations ranging from 0.01 ng to 100 μg in T75 flasks with screwcap septa (MIDSCI, St. Louis, MO). The flasks were manually agitated for 10 seconds and the screw cap septum was punctured by a needle ...
-
No products found
because this supplier's products are not listed.
Michael J. Grey, et al.,
bioRxiv - Cell Biology 2021
Quote:
Co-housed 8-week-old WT and Ern2-/- mice were interperitoneally injected daily for four days with 10 μmol/kg Dibenzazepine (DBZ, previously Syncom, now Chiralix, Nijmegen, Netherlands) suspended in 0.5% (w/v ...
-
No products found
because this supplier's products are not listed.
Ana Kucera, et al.,
bioRxiv - Immunology 2020
Quote:
... T cell cultures were re-stimulated with DCs and 10 days later T cells were stained with MART-1 dextramer (Immudex, Copenhagen, Denmark) to assess the presence of MART-1 antigen-specific T cells in the cultures.
-
No products found
because this supplier's products are not listed.
Renyuan Zhang, et al.,
bioRxiv - Pathology 2022
Quote:
... Cytokine signaling mediating castration was assessed using either a combination of a TGFβR1/ALK5 inhibitor (SB431542, Stemgent, 10 mg/kg) and a p38 inhibitor (BIRB 796, Biotang, 3.33 mg/kg), by daily subcutaneous injection one day prior to castration and every day thereafter for the duration of the experiment ...
-
No products found
because this supplier's products are not listed.
Kristi Dietert, et al.,
bioRxiv - Neuroscience 2022
Quote:
... A total of 21,600 cells were plated onto the upper wells (n=6/condition) of 96-well poly-l-ornithine (PLO) coated chemotaxis chambers (10 μm pore size, Neuro Probe Inc) and maintained in DMEM containing l-glutamine (1 mM) ...
-
No products found
because this supplier's products are not listed.
Yalda Afshar, et al.,
bioRxiv - Cell Biology 2022
Quote:
... The flushed cells were subsequently collected and collagenase was inactivated with the addition of equivalent volume 10% feta bovine serum (FBS) with MCDB 131 media (VEC Technologies, Rensselaer, NY). The cells were pelleted ...
-
No products found
because this supplier's products are not listed.
Alyssa R. Phillips, et al.,
bioRxiv - Plant Biology 2023
Quote:
... About 1 mm of the tip (meristem and root cap) was excised and transferred to a tube containing 20 µL of 3% cellulase R-10 (Desert Biologicals, Phoenix, AZ) and 1.25% pectolyase Y-23 (Desert Biologicals ...
-
No products found
because this supplier's products are not listed.
Jessica Lacoste, et al.,
bioRxiv - Cell Biology 2023
Quote:
... capillary columns were pulled with a laser puller and packed in-house with 10–12 cm C18 (Reprosil-Pur 120 C18-AQ, 3 μm, Dr. Maisch HPLC GmbH) in methanol ...
-
No products found
because this supplier's products are not listed.
Latha Muniappan, et al.,
bioRxiv - Pathology 2020
Quote:
Calpain-2 immunostaining was performed using a rabbit anti-human calpain-2 (10 µg/ml, catalog No. RP-3 Calpain-2; Triple Point biologics, Forest Grove, OR). CD68 immunostaining was performed using a rabbit anti-human CD68 antibody (1:200 ...
-
No products found
because this supplier's products are not listed.
Stephanie N. Caty, et al.,
bioRxiv - Microbiology 2024
Quote:
... frogs were rinsed with sterile water and then swabbed 10 times (up and back is once) on the dorsal side using Puritan Sterile Tipped Polyester Applicators (Puritan Medical Products, 25-206 1PD BT). Alkaloid feeding began 5 days after the onset of swabbing (Day 13 ...
-
No products found
because this supplier's products are not listed.
Kunal Sikder, et al.,
bioRxiv - Cell Biology 2023
Quote:
... nCMs cells were plated onto 10 μg/ml laminin-coated wells with 12 kPa or 50 kPa hydrogels (Matrigen cat# SW12-EC-12 PK and SW12-EC-50 PK). To prevent the potential growth of non-myocyte cells ...