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No products found
because this supplier's products are not listed.
Takanori Matsubara, et al.,
bioRxiv - Neuroscience 2020
Quote:
Whole-cell patch-clamp recordings from cultured HEK 293 cells or VTA-DA neurons in acute brain slices were performed using an IPA amplifier (Sutter Instruments) at RT ...
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No products found
because this supplier's products are not listed.
Wenning Chu, et al.,
bioRxiv - Bioengineering 2023
Quote:
... flowthrough and elution fractions collected as described in Section 2.6 were determined using a HEK 293 HCP ELISA kit (Cygnus Technologies, Southport, NC) following the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Amado Carreras-Sureda, et al.,
bioRxiv - Biochemistry 2021
Quote:
... STIM1 cherry (in HEK-293 cells) or TCR clusters were imaged using a ZET 561/10 excitation filter (Chroma Technology Corp., Bellows Falls, VT). SiR-Actin was measured using 640 nm laser line ...
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No products found
because this supplier's products are not listed.
Michael JV White, et al.,
bioRxiv - Bioengineering 2022
Quote:
... and VWF-A3 were fluorescently labeled with Cy7 using sulfo-Cy7 N-hydroxysuccinimide ester (Lumiprobe) according to the manufacturer’s instruction ...
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No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... 5 μg of His-tagged H2A:H2B or His-H3:Flag-H4 (Diagenode) and 2 μg of Flag-hSpt16 were added to 250 μl of binding buffer (50 mM Tris-HCl pH 7.4 ...
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No products found
because this supplier's products are not listed.
Julien Hazemann, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... 0.03 M each of magnesium chloride and calcium chloride (condition A3 of Morpheus I screen, Molecular Dimensions Ltd).
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No products found
because this supplier's products are not listed.
Peter Hanna, et al.,
bioRxiv - Neuroscience 2020
Quote:
... A quadripolar His catheter (Abbott) was placed via the right external jugular vein and advanced until a His signal was visualized while a quadripolar catheter was inserted via the right femoral vein and advanced to the right atrium ...
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No products found
because this supplier's products are not listed.
Derek C. Rookhuizen, et al.,
bioRxiv - Immunology 2021
Quote:
... 45µl of room temperature Trans-IT 293 Transfection Reagent (Euromedex, Mirus 2700) was then added and the mixture incubated at RT for 30 minutes ...
-
No products found
because this supplier's products are not listed.
Julian Schöllkopf, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
N-terminally His-tagged Pasteurella multocida toxin (PMT) was coated at 10 µg/mL (500 ng/well ...
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No products found
because this supplier's products are not listed.
David M. Zuckerman, et al.,
bioRxiv - Microbiology 2022
Quote:
... The proteins were injected into rabbits to generate polyclonal antibodies according to standard protocols (His-GspD, Cocalico, Reamstown, PA; His-PilQOlut, Eurogentec, Seraing, BE). Sera were tested for cross-reactivity by immunoblotting lysates from wildtype M ...
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No products found
because this supplier's products are not listed.
Luyao Huang, et al.,
bioRxiv - Plant Biology 2023
Quote:
... the purified recombinant mCherry or Tip6ΔSP-mCherry proteins were separately mixed with purified His-ZmTPL2N-His proteins and incubated with pre-washed magnetic mCherry-Trap beads (Chromotek, Cat. No. rtmak) in wash buffer (0.2 M NaH2PO4/0.2 M Na2HPO4 ...
-
PIK-293 is a PI3K inhibitor, mostly for PI3Kδ with IC50 of 0.24 μM, 500-, 100- and 50-fold less...
Cat# S2207, SKU# S2207-5mg,
5mg, $170.00
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Gahyang Cho, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... transfected HEK cells were treated with a proteasome inhibitor (20 μM MG132, Selleck Chemicals, TX) for 2 h ...
-
No products found
because this supplier's products are not listed.
Yeyun Ouyang, et al.,
bioRxiv - Cell Biology 2022
Quote:
HEK 293T and A375 cells were seeded on 35 mm Fluorodish plates (World Precision Instruments). 24 hr later ...
-
No products found
because this supplier's products are not listed.
Abhinav Parivesh, et al.,
bioRxiv - Cell Biology 2022
Quote:
HEK-293T cells were seeded at 2.5×106 in 25ml of DMEM (Genesee Cat. 25-500) completed with 10% heat-inactivated FBS (Thermo Cat ...
-
No products found
because this supplier's products are not listed.
Kari H. Ecklund, et al.,
bioRxiv - Cell Biology 2021
Quote:
Anti-His-coated 0.44 μm microbeads (PSS4; Spherotech; prepared as described previously72) were incubated with purified 6His-GFP-3HA-GST-dynein331-HALO in dynein trapping buffer (30 mM HEPES pH 7.2 ...
-
No products found
because this supplier's products are not listed.
Ben S. Ou, et al.,
bioRxiv - Bioengineering 2023
Quote:
... HIS Lite Cy3 Bis NTA-Ni Complex was purchased from AAT Bioquest. Unless otherwise stated ...
-
No products found
because this supplier's products are not listed.
Advika Kamatar, et al.,
bioRxiv - Biophysics 2023
Quote:
... His-Clathrin was labeled with Atto594 NHS-ester (ATTO-TEC, Sigma-Aldrich) according to a previously published protocol (41 ...
-
No products found
because this supplier's products are not listed.
Surbhi Verma, et al.,
bioRxiv - Microbiology 2023
Quote:
... Human MDMs (hMDMs) were isolated from human PBMCs and separated from healthy human donors’ blood using PolymorphPrep (PROGEN,1895) layering and centrifugation ...
-
No products found
because this supplier's products are not listed.
Gabrielle Brandt, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... recombinant human transferrin (InVitria), 80 μg/mL ...
-
No products found
because this supplier's products are not listed.
Xiaoyu Shi, et al.,
bioRxiv - Bioengineering 2021
Quote:
... and HEK 293T cells were seeded at 1 × 104 cells/cm2 in 16-well chambers (Grace Bio-Labs, 112358) and grown to 75% confluency.
-
No products found
because this supplier's products are not listed.
Yoichi Araki, et al.,
bioRxiv - Neuroscience 2023
Quote:
Live HEK 293T cells and cultured hippocampal neurons were imaged using a Cell Observer spinning disk confocal microscope (Carl Zeiss) or an LSM 880 laser scanning confocal microscope (Carl Zeiss) ...
-
No products found
because this supplier's products are not listed.
Tracy J. Berg, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Primary human astrocytes (3H Biomedical) were cultured in Astrocyte Medium (3H Biomedical ...
-
No products found
because this supplier's products are not listed.
Marin Boutonnet, et al.,
bioRxiv - Neuroscience 2023
Quote:
... human angiotensin II (HelloBio®); N-Methyl-D-Aspartate (NMDA ...
-
No products found
because this supplier's products are not listed.
Sandrine Huot, et al.,
bioRxiv - Immunology 2020
Quote:
... Human MPO and human Lactoferrin ELISA kits were purchased from Assaypro (St. Charles, MO, USA). MMP-9 Duoset ELISA was obtained from R&D Systems ...
-
No products found
because this supplier's products are not listed.
Dalia Rosano, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... HEK-293T cells were transfected with cellecta CloneTracker XP library and ready-to-use lentiviral packaging plasmid mix (Cellecta, CPCP-K2A) using Lipofectamine (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Marcel Rak, et al.,
bioRxiv - Biochemistry 2023
Quote:
Co-crystallization trials were performed using the sitting-drop vapor-diffusion method at 293 K with a mosquito crystallization robot (TTP Labtech, Royston UK). MST3 (11 mg/mL in 25 mM HEPES pH 7.5 ...
-
No products found
because this supplier's products are not listed.
Anna K. Hundsdoerfer, et al.,
bioRxiv - Genomics 2023
Quote:
... euphorbiae (from one head each; the same individuals used for the PacBio genome and Hi-C sequencing) and neural tissue from the internal reference standard Acheta domesticus (female ...
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No products found
because this supplier's products are not listed.
Lior Tal, et al.,
bioRxiv - Plant Biology 2023
Quote:
... Arabidopsis KAI2 protein was expressed as a 6× His-SUMO fusion protein using the expression vector pSUMO (LifeSensors). His-SUMO-KAI2 was isolated from by Ni-NTA resin and the eluted His-SUMO KAI2 was further separated by anion-exchange ...
-
No products found
because this supplier's products are not listed.
Mohita Tagore, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... mouse anti-human GPHN (Synaptic Systems #147011); rabbit anti-human KRT17 (Sigma #HPA000452) ...
-
No products found
because this supplier's products are not listed.
Alicja Przybyszewska-Podstawka, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... TGFβ (human TGFβ1, Biorbyt, Cambridge, United Kingdom), Gibson Assembly® Master Mix (NEB) ...
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No products found
because this supplier's products are not listed.
Antje Neeb, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... panBAG-1 (human, rabbit monoclonal, RM356, RevMAb), panBAG-1 (mouse ...
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No products found
because this supplier's products are not listed.
Nazila V. Jafari, Jennifer L. Rohn,
bioRxiv - Microbiology 2022
Quote:
HBLAK human bladder progenitor cells (CELLnTEC, Switzerland) were grown according to the CELLnTEC protocol ...
-
No products found
because this supplier's products are not listed.
Alina Remeeva, et al.,
bioRxiv - Biophysics 2021
Quote:
... with the mutation C85A and C-terminal 6×His tag) was obtained as a synthetic gene from Evrogen (Russia) and introduced into the pET11 expression vector (Novagen ...
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No products found
because this supplier's products are not listed.
Maurizio Chioccioli, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... using human Col1a1 and Acta2 primers from ABI for the specified gene and species.
-
No products found
because this supplier's products are not listed.
M Azharuddin, et al.,
bioRxiv - Immunology 2021
Quote:
... or anti-human IgA-HRP (Nordic BioSite, Täby, Sweden) was added to separate wells with diluted serum samples and incubated 90 min ...
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No products found
because this supplier's products are not listed.
Ava P. Soleimany, et al.,
bioRxiv - Bioengineering 2020
Quote:
Fresh frozen human PCa TMAs (BioChain Institute, Inc.; T6235201) were air dried and fixed in ice-cold acetone for 10 minutes ...
-
No products found
because this supplier's products are not listed.
Astrid Hendriks, et al.,
bioRxiv - Microbiology 2023
Quote:
... Human neutrophils were freshly isolated using Polymorphprep (Alere technologies) per manufacturer instructions ...
-
No products found
because this supplier's products are not listed.
Tommy Tong, et al.,
bioRxiv - Immunology 2023
Quote:
... followed by anti-human IgG AP conjugate (Accurate Chemicals) and were developed using SigmaFast BCIP/NBT ...
-
WB, ELISA
Cat# CDC-41,
0.1 mg, Inquire
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Jared M. Fischer, et al.,
bioRxiv - Bioengineering 2024
Quote:
... A375-Luc/iRFP (human melanoma, Creative Biogene CSC-RR0254), MCF7 (human breast cancer ...
-
No products found
because this supplier's products are not listed.
Asha A. Philip, John T. Patton,
bioRxiv - Microbiology 2022
Quote:
... A puc19 plasmid containing a RIX/NSP3-2A-P-His insert under the control of a T7 transcription promoter (puc19/T7/ RIX/NSP3-2A-P-His) was purchased from Bio Basic Canada Inc ...
-
No products found
because this supplier's products are not listed.
Oskar Staufer, et al.,
bioRxiv - Immunology 2022
Quote:
... beads were incubated with varying amount of the recombinant his-tagged proteins and corresponding MFIs were compared to reference beads of know AlexFluor molecule densities (Bangs Laboratories, IN).
-
No products found
because this supplier's products are not listed.
Leslie E. Lupien, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... human DiI-VLDLs (1 mg protein/mL; Alfa Aesar Chemicals), LPL from bovine milk (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Piyakarn Boontem, Tetsumori Yamashima,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... rabbit anti-human activated μ-calpain antibody (PEPTIDE Institute, Japan) at 1:250 overnight ...
-
No products found
because this supplier's products are not listed.
Lorenz Thurner, et al.,
bioRxiv - Immunology 2021
Quote:
... rabbit anti human IL-1-Ra-Abs (antibodies-online # ABIN2856394) or mouse anti-human PGRN-Abs (abcam#ab169325 ...
-
No products found
because this supplier's products are not listed.
Yekyung Seong, et al.,
bioRxiv - Immunology 2021
Quote:
Anti-human CD11b (Clone M1/70, Leinco Technologies, Fenton, MO) was conjugated with Alexa Fluor 532 NHS ester according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Mutsumi Kobayashi, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Human iPSCs were dissociated using Accutase (Innovative Cell Technologies, AT104) and suspended in mTeSR plus (Stemcell Technologies ...
-
No products found
because this supplier's products are not listed.
Siyuan Hao, et al.,
bioRxiv - Cell Biology 2022
Quote:
Human MYC_intron with Quasar 570 dye (Biosearch Technologies, ISMF-2066-5), human ACTB_intron with Quasar 570 dye (Biosearch Technologies ...
-
No products found
because this supplier's products are not listed.
Lucia Pedicini, et al.,
bioRxiv - Cell Biology 2020
Quote:
... IDA data were searched against human database in ProteinPilot 4.5 (AB-SCIEX, Canada).
-
No products found
because this supplier's products are not listed.
Ragini Phansalkar, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... with probe for human TINAGL1(Advanced Cell Diagnostics #857221-C2) and OPAL 570 fluorophore (Akoya #FP1488001KT), following the manufacturer’s protocol.
-
No products found
because this supplier's products are not listed.
Blasi Maria, et al.,
bioRxiv - Immunology 2020
Quote:
... Plates were then incubated with 2 μg/ml biotinylated rabbit anti-human IFN-γ (U-CyTech biosciences, Utrecht, The Netherlands) for 2 hours at room temperature ...