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SARS-CoV-2, Recombinant Protein, S1 Subunit Protein [sheep Fc].
Cat# VReP-Wyb139,
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Xiaotian Tan, et al.,
bioRxiv - Bioengineering 2020
Quote:
... The human-cell-expressed SARS-CoV Spike S1-His recombinant protein was purchased from Creative Biolabs (VAng-Wyb7337). The recombinant CR3022 therapeutic antibody was purchased from Creative Biolabs (MRO-1214LC) ...
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No products found
because this supplier's products are not listed.
Yisong Qian, et al.,
bioRxiv - Immunology 2021
Quote:
... SARS-CoV-2 papain-like protease (DB604) was purchased from Lifesensors (Malvern, PA). HCoV-HKU1 coronavirus nucleocapsid protein ...
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No products found
because this supplier's products are not listed.
James M. Burke, et al.,
bioRxiv - Immunology 2021
Quote:
... and SARS-CoV-2 smFISH probes were designed using Stellaris smFISH probe designer (Biosearch Technologies) available online at http://www.biosearchtech.com/stellaris-designer ...
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No products found
because this supplier's products are not listed.
Bin Zhou, et al.,
bioRxiv - Microbiology 2020
Quote:
... RNA was extracted from Vero E6 cells infected with either recombinant SARS-CoV-2S-614D or SARS-CoV-2S-614G using the NucleoSpin RNA Plus kit (Macherey-Nagel) according to the manufacturer’s guidelines ...
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No products found
because this supplier's products are not listed.
Kristin Metzdorf, et al.,
bioRxiv - Immunology 2022
Quote:
... 3 μm thick FFPE sections were stained for SARS-CoV-2 with mouse-anti-nucleocapsid CoV-1/2 (Synaptic Systems, HS-452 11, clone 53E2, subtype: IgG2a) and for macrophages with rat-anti-mouse-MAC2 (Biozol Diagnostica/CEDARLANE,CL8942AP ...
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No products found
because this supplier's products are not listed.
Mehdi Moustaqil, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... coli C41(DE3) cells were transformed with the SARS-CoV-2 pET-28a-nsp5 plasmid (IDT DNA). Single colonies were used to inoculate LB media supplemented with kanamycin (50 μg/mL) ...
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No products found
because this supplier's products are not listed.
Anastasia Polycarpou, et al.,
bioRxiv - Immunology 2023
Quote:
UV inactivation of virus was performed by exposure of SARS-CoV-2 (England 02/2020) at a maximal depth of 1.5mm in 6-well plates (Sarstedt) placed on ice ...
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No products found
because this supplier's products are not listed.
Hang Ma, et al.,
bioRxiv - Cell Biology 2021
Quote:
... the binding of antibodies with SARS-CoV-2 antigens were visualized by adding 100 μL peroxidase substrate TMB Single-Component Substrate solution (Solarbio) and incubating for 15 min in dark ...
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No products found
because this supplier's products are not listed.
Claire T. Deakin, et al.,
bioRxiv - Immunology 2021
Quote:
Full-length SARS-CoV-2 and HCoV-OC43 nucleoproteins were produced by expression in Escherichia coli C43(DE3) cells (Lucigen) and purified as previously described (12) ...
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No products found
because this supplier's products are not listed.
Robert V. Blair, et al.,
bioRxiv - Pathology 2020
Quote:
Samples collected at preinfection and weekly post-infection until necropsy were tested for detection of binding IgG antibodies against SARS-CoV-2 nucleoprotein (NP) by MFIA COVID-Plex from Charles River Laboratories ...
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No products found
because this supplier's products are not listed.
Nicholas You Zhi Cheang, et al.,
bioRxiv - Immunology 2023
Quote:
... were stimulated in a 96-well round-bottom plate with 5 µg/mL ancestral and variant SARS-CoV-2 RBD peptides and 1 µg/mL anti-CD28 (40-0281-M001; Tonbo Biosciences, San Diego, California, USA) at 37°C and 5% CO2 ...
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No products found
because this supplier's products are not listed.
David Swygart, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Spike trains were recorded using glass pipettes (2–3MΩ) filled with AMES solution in cell-attached configuration ...
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No products found
because this supplier's products are not listed.
James R. Byrnes, et al.,
bioRxiv - Immunology 2021
Quote:
... beads were collected and glycoproteins reduced with 5 mM TCEP (Calbiochem) for 30 minutes at 37°C and alkylated with 11 mM iodoacetamide (Sigma ...
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No products found
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Erica Bello, et al.,
bioRxiv - Neuroscience 2023
Quote:
... coli Spike-in DNA (EpiCypher) was added to each sample to normalise reads to control for experimental variability and sequencing depth ...
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No products found
because this supplier's products are not listed.
Shuo Han, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Biotin phenol (Iris Biotech) was dissolved in DMSO as 500 mM stock solution and added directly to cell culture media to a final concentration of 500 μM ...
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No products found
because this supplier's products are not listed.
Martina Hauke, et al.,
bioRxiv - Microbiology 2023
Quote:
... and 10% FCS (PromoCell, Germany). HEK-NF-κB_luc were supplemented for routine growth with 50 µg/mL Hygromycin B (Invivogen ...
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No products found
because this supplier's products are not listed.
Ashley A. Johnson, et al.,
bioRxiv - Physiology 2021
Quote:
... Spectra were measured from HEK293 cells using a 60x objective with NA 1.45 (Nikon) and an inverted microscope (Nikon TE-2000) ...
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No products found
because this supplier's products are not listed.
Julius Brandenburg, et al.,
bioRxiv - Immunology 2020
Quote:
... 10% of heat-inactivated fetal calf serum (FCS; Pan-Biotek, Aidenbach, Germany), supplemented with 50 ng/ml macrophage colony stimulating factor (M-CSF ...
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No products found
because this supplier's products are not listed.
Sebastian Tacke, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... plain grids (Quantifoil 2/2) were transferred to the microscope ...
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No products found
because this supplier's products are not listed.
Sangam Kandel, et al.,
bioRxiv - Genomics 2023
Quote:
... Samples were tested for the SARS-CoV-2 using the Aptima® SARS-CoV-2 (Panther® System, Hologic, San Diego, CA) nucleic acid amplification assay ...
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No products found
because this supplier's products are not listed.
Huanzhou Xu, et al.,
bioRxiv - Microbiology 2020
Quote:
... rabbit anti-SARS-CoV-2 ORF3a antibody (FabGennix, Cat. SARS-COV2-ORF3A-101AP), HRP-conjugated goat anti-mouse IgG(H+L ...
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No products found
because this supplier's products are not listed.
Chamandi S. Dampalla, et al.,
bioRxiv - Microbiology 2021
Quote:
... SARS-CoV-2 3CLpro complex with compound 2: Index HT screen (Rigaku Reagents) condition G8 (25% (w/v ...
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No products found
because this supplier's products are not listed.
Dalia Raïch-Regué, et al.,
bioRxiv - Microbiology 2022
Quote:
... The amount of SARS-CoV-2 nucleoprotein released to the supernatant was measured with SARS-CoV-2 nucleocapsid protein High-Sensitivity Quantitative ELISA (ImmunoDiagnostics) according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Chamandi S. Dampalla, et al.,
bioRxiv - Microbiology 2021
Quote:
... SARS-CoV 3CLpro complex with compound 2: Index HT screen (Hampton Research) condition H4 (30% (w/v ...
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No products found
because this supplier's products are not listed.
Mohamed Fareh, et al.,
bioRxiv - Microbiology 2020
Quote:
... SARS-CoV-2 RNA was converted to cDNA using the SensiFAST cDNA kit (#BIO-65053, BioLine) with 10 μL of RNA extract per reaction following the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Ross N. England, et al.,
bioRxiv - Immunology 2023
Quote:
... diproline-modified spike protein from the SARS-CoV-2 Wuhan-Hu-1 strain were produced as previously described15,16 with m1Ψ-5-triphosphate (TriLink) instead of UTP and capped cotranscriptionally using the trinucleotide cap1 analog ...
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No products found
because this supplier's products are not listed.
Longlong Si, et al.,
bioRxiv - Microbiology 2020
Quote:
... Similar results were also obtained using a commercially available pseudotyped SARS-CoV-2 S protein expressing viral particles (Amsbio LLC).
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No products found
because this supplier's products are not listed.
Ghada Elfayres, et al.,
bioRxiv - Microbiology 2023
Quote:
HEK293T cells were seeded into 10-cm plates 24 h prior to transfection with pEZY3 plasmids expressing SARS-CoV-2 proteins using polyethyleneimine (PEI; PolyScience). For this ...
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No products found
because this supplier's products are not listed.
Irina A. Favorskaya, et al.,
bioRxiv - Immunology 2021
Quote:
The RBD nucleotide sequence of SARS-CoV-2 Wuhan-Hu-1 isolate (Genbank accession number MN908947, from 319 to 545 aa) was synthesized (Evrogen, Russia) and cloned into the pCEP4 mammalian expression vector (Thermo Fisher Scientific ...
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No products found
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Winston Chiu, et al.,
bioRxiv - Immunology 2022
Quote:
... the plates were transferred to the BSL-3+ Caps-It robotics system where 30 µL SARS-CoV-2 was added using a noncontact liquid handling system (Tecan, EVO 100) to achieve a multiplicity of infection (MOI ...
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No products found
because this supplier's products are not listed.
Rodney G King, et al.,
bioRxiv - Immunology 2020
Quote:
... recombinant SARS-CoV-2 ectodomain trimers were passively absorbed onto streptavidin functionalized fluorescent microparticles (Spherotech 3.6um cat# CPAK-3567-4K, peak 4). 500 µg of biotinylated SARS2-CoV-2 was incubated with 2E+07 Streptavidin functionalized fluorescent microparticles in 400μl of 1% BSA in PBS ...
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No products found
because this supplier's products are not listed.
Denise Pajonczyk, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
ERK1/2 phosphorylation in HEK293 FPR cell systems was measured with the HTRF immunoassay from Cisbio, as described previously [37] ...
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No products found
because this supplier's products are not listed.
Navid Paknejad, Vinay Sapuru, Richard K. Hite,
bioRxiv - Biophysics 2022
Quote:
... Membrane proteins were solubilized from 2.4 L of pelleted HEK293S GnTI-cells expressing wild-type hIP3R3 for 2 hours by rotation in 2% lauryl maltose neopentyl glycol (LMNG; Anatrace Cat# NG310), 150 mM sodium chloride (NaCl) ...
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No products found
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Jingwen Niu, et al.,
bioRxiv - Neuroscience 2020
Quote:
... sheep anti-NGF (CedarLane, catalog #CLMCNET-031), Rabbit anti-phosphoMKK4 (Cell Signaling Technology ...
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No products found
because this supplier's products are not listed.
Jennifer L. Reedy, et al.,
bioRxiv - Immunology 2022
Quote:
... Dectin-2(murine)-IgG1 Fc (human) fusion protein (ENZO Life Sciences, Inc, Farmingdale, NY) or IgG1 Fc (human ...
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No products found
because this supplier's products are not listed.
Jerry Yan, et al.,
bioRxiv - Bioengineering 2023
Quote:
... plated at 800,000 splenocytes/well and restimulated with 10 μg rabies virus glycoprotein peptide (AnaSpec) for 24h at 37 °C ...
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No products found
because this supplier's products are not listed.
David McClellan, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... Biotin was obtained from TCI Chemicals and doxycycline (doxy ...
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No products found
because this supplier's products are not listed.
James Brett Case, et al.,
bioRxiv - Microbiology 2020
Quote:
... Secreted hACE2-Fc and mACE2-Fc proteins were purified by protein A chromatography (Goldbio).
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No products found
because this supplier's products are not listed.
Till Stephan, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Spot tag (ChromoTek, Planegg-Martinsried, Germany), TOMM22 (Merck ...
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No products found
because this supplier's products are not listed.
Sarah J. Jefferson, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The mouse was briefly anesthetized with isofluorane and the magnetic ear tag was placed on a mouse’s ear using the ear tag applicator (#56791 Stoelting). Each mouse received one ear tag because signal was sufficiently strong with one tag and ears tended to get stuck together when both received ear tags ...
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No products found
because this supplier's products are not listed.
Tobias Kipura, et al.,
bioRxiv - Biochemistry 2024
Quote:
... QC and standard samples were spiked with ISTDs (Cambridge Isotope Laboratories, Table S1) to a final concentration of 1.25 µM to calculate the response ratio (peak area 12C/peak area 13C).
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No products found
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Scott P. Davies, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... supplemented with 10% fetal calf serum (FCS, EuroClone) (complete medium) ...
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No products found
because this supplier's products are not listed.
Kenneth W. Latimer, et al.,
bioRxiv - Neuroscience 2019
Quote:
... For S1 recordings pipettes (1.5 mm outer diameter, 0.86 inner diameter, BF150-86-10, Sutter instruments) were pulled on a PC-10 vertical puller (Narashige ...
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Cat# HY-P7435-50 μg,
50 μg, USD $375.0
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Yang Meng, et al.,
bioRxiv - Microbiology 2022
Quote:
... 100 μg/ml or 200 μg/ml 5-FC (MedChemExpress), 0.5 μg/ml or 1.0 μg/ml Amp B (MedChemExpress ...
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No products found
because this supplier's products are not listed.
Siyi Gu, et al.,
bioRxiv - Cell Biology 2023
Quote:
HEK293 cells expressing Flag-SNAP-CCR5 were seeded onto 10-mm glass-bottom dishes (FluoroDish, FD3510, WPI) previously coated with 5 μg/ml fibronectin ...
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No products found
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Binayak Sarkar, et al.,
bioRxiv - Microbiology 2024
Quote:
... while DAGs/TAGs were resolved using a Luna 5U C-5 column (Phenomenex) using established solvent systems.
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No products found
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Alba Ventos-Alfonso, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... the bisulfite conversion ratio was calculated using spike-in controls provided by Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit (Diagenode) (supplementary Table S2 ...
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No products found
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Maayan Eilon-Ashkenazy, et al.,
bioRxiv - Microbiology 2024
Quote:
A purified LUJV spike sample (3.5 μl) was applied on glow-discharged (6 s, 12 mA; Pelco easiGlow, Ted Pella) graphene oxide Quantifoil copper grids ...
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No products found
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Hana Saoud, et al.,
bioRxiv - Neuroscience 2021
Quote:
... It was possible to identify neonates by means of their three-digit metal ear tags (ref. 52-4716, Harvard Apparatus, Les Ulis, France). After surgery ...
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No products found
because this supplier's products are not listed.
Udoka C. Nwangwu, et al.,
bioRxiv - Zoology 2024
Quote:
... 15 ml Fetal Calf Serum (FCS) 3% and 5ml Amphotericin B) and 500ml of Zirconia beads (Firma Biospec: 2.0 mm, Cat. No 1107912). The contents were macerated using the Qiagen Tissuelyser LT for 10 min and then centrifuged at 4,500 x g for 15 min ...