-
No products found
because this supplier's products are not listed.
Venkatesh Kumaresan, et al.,
bioRxiv - Immunology 2024
Quote:
... or (E)-2-Benzylidene-3-(cyclohexylamino)-2,3-dihydro-1H-inden-1-one (BCI, DUSP 1/6 inhibitor, Cat# 317496, Sigma) at different concentrations as indicated for each experimental condition ...
-
No products found
because this supplier's products are not listed.
Lauren J. Lahey, et al.,
bioRxiv - Biochemistry 2020
Quote:
... 4-[(2-Butyl-6,7-dichloro-2-cyclopentyl-2,3-dihydro-1-oxo-1H-inden-5-yl)oxy] butanoic acid (DCPIB) was purchased from Tocris Bioscience and reconstituted to 50 mM in DMSO ...
-
No products found
because this supplier's products are not listed.
Nancy G. Azizian, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... were purchased from Click Chemistry Tools and Tris [(1-benzyl-1H-1, 2, 3-triazol-4-yl)methyl] amine from Fisher Scientific.
-
No products found
because this supplier's products are not listed.
Joseph O. Magliozzi, James B. Moseley,
bioRxiv - Cell Biology 2021
Quote:
... all strains were grown in YE4S at 32°C and treated with 30 μM 3-Brb-PP1 (3-[(3-Bromophenyl)methyl]-1-(1,1-dimethylethyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine (Abcam) for 1 h and fixed in ice-cold 70% ethanol ...
-
No products found
because this supplier's products are not listed.
Krzysztof Mikołajczyk,
bioRxiv - Biochemistry 2024
Quote:
... and untransfected cells were cultured for 2 weeks in a complete medium containing 3 μM N-[(1R,2R)-1- (2,3-dihydro-1,4-benzodioxin-6-yl)-1-hydroxy-3-pyrrolidin-1-ylpropan-2-yl]nonanamide (Genz-123346) (Merck, Darmstadt, Germany), a glucosylceramide synthase inhibitor [26].
-
No products found
because this supplier's products are not listed.
Caleb Ruiz-Jiménez, et al.,
bioRxiv - Immunology 2021
Quote:
... was incubated with 50μl MTT (sodium 3′-[1-(phenylaminocarbonyl)-3,4-tetrazolium]-bis (4-methoxy-6-nitro) benzene sulfonic acid hydrate) labeling reagent (Roche Life Science, USA) to each well ...
-
No products found
because this supplier's products are not listed.
Qixuan Weng, et al.,
bioRxiv - Genetics 2024
Quote:
... a 10 mM stock of 1-(1,1-dimethylethyl)-3-(1-naphthalenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine (1-NA-PP1; Cayman Chemical 10954) in DMSO was thawed on ice and added to a final concentration of 15 µM ...
-
No products found
because this supplier's products are not listed.
Robert Parker, et al.,
bioRxiv - Immunology 2020
Quote:
... and CD11c (S-HCL-3, Biolegend) for 20 minutes at room temperature ...
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No products found
because this supplier's products are not listed.
Beatrice Dyring-Andersen, et al.,
bioRxiv - Cell Biology 2020
Quote:
... APC–conjugated CD11c (S-HCL-3, BD Biosciences), BV605–conjugated CD3 (OKT3 ...
-
No products found
because this supplier's products are not listed.
Tina Wiegand, et al.,
bioRxiv - Biophysics 2024
Quote:
... 0.1 % 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)-5000] (ammonium salt) (PEG5000 PE) and 0.01 %DiL (all Avanti Polar Lipids). Lipids were dissolved in chloroform ...
-
No products found
because this supplier's products are not listed.
Hazal Kübra Gürkaşlar, Ingrid Hoffmann,
bioRxiv - Cell Biology 2024
Quote:
... and they were selected with 2 and 5 μg/ml Blasticidin S HCl (Santa Cruz Biotechnology). Colonies were screened for CEP152 knock-out and CEP152-AID expression ...
-
No products found
because this supplier's products are not listed.
Madlen Stephani, et al.,
bioRxiv - Cell Biology 2020
Quote:
... were amine coupled on to a Series S CM5 sensor chip (GE Healthcare) using 2 adjacent flow cells (i.e ...
-
No products found
because this supplier's products are not listed.
Ryuta Niikura, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... 1% P/S and 2 ng/mL human IL-3 (PeproTech, AF-300-23). TF-1 cells52 were cultured in RPMI-1640 medium supplemented with 10% FBS ...
-
No products found
because this supplier's products are not listed.
Jason C. Collins, et al.,
bioRxiv - Biochemistry 2023
Quote:
... blocked in 2% BSA for 1h and stained with primary anti-UBA1a/b antibodies (Cell Signaling, 4891S, 1:500) for 1h ...
-
No products found
because this supplier's products are not listed.
Anne Billet, et al.,
bioRxiv - Biochemistry 2023
Quote:
... HCTU (O-(1H-6-chlorobenzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate) from VWR, dichloromethane (DCM) ...
-
No products found
because this supplier's products are not listed.
Carmen de Pablo, Sergio Casas-Tintó,
bioRxiv - Cancer Biology 2023
Quote:
... DNA was stained with 2-(4-amidinophenyl)-1H-indole-6-carboxami-Dine (DAPI) at 1 μM in Vectashield mounting media (Vector Laboratories).
-
No products found
because this supplier's products are not listed.
Yasuhiro Fujiwara, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The expressed recombinant protein was solubilized in a denaturing buffer (6 M HCl-Guanidine, 20 mM Tris-HCl[pH 7.5] and purified by Ni-NTA (30210, QIAGEN) under denaturing conditions ...
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No products found
because this supplier's products are not listed.
John B. Moldovan, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... approximately 2-3×105 cells were plated per well in 6-well plates (Corning) and transfected with 0.25 μg of “driver” plasmid (pTMO2F3 ...
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No products found
because this supplier's products are not listed.
Stamatis Papathanasiou, et al.,
bioRxiv - Cell Biology 2022
Quote:
... U2OS 2-6-3 were transduced with pLenti CMV rtTA3 Blast (w756-1, plasmid #26429; Addgene, gift from E. Campeau) for the expression of rtTA ...
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No products found
because this supplier's products are not listed.
Fabian Link, et al.,
bioRxiv - Cell Biology 2023
Quote:
HaloTag-Ligand-Amine (Promega, #P6741) was reconstituted in DMF (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Ankan Gupta, et al.,
bioRxiv - Neuroscience 2024
Quote:
... and 6 m/s velocity (Leica MyNeuroLab Impact One Stereotaxic Impactor ...
-
No products found
because this supplier's products are not listed.
Alison L. Wong, et al.,
bioRxiv - Neuroscience 2021
Quote:
... 3% paraformalydehyde (15754-S, EMS) and 0.1 M Sorensen’s phosphate buffer pH 7.2 solution ...
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No products found
because this supplier's products are not listed.
Gunnar Sørensen, et al.,
bioRxiv - Neuroscience 2021
Quote:
... sections were rinsed and incubated with biotinylated rabbit anti-rat IgG for 1h (1:200, DAKO Cytomation A/S, Denmark). Following avidin-biotin-peroxidase complex (Vector laboratories ...
-
No products found
because this supplier's products are not listed.
M. Eugenia Dieterle, et al.,
bioRxiv - Microbiology 2020
Quote:
... coated with PBS alone or with 2 µg/ml of SARS-CoV-2 S protein in PBS overnight at 4°C were blocked for 1 hour with 3% nonfat dry milk (Biorad) in PBS ...
-
No products found
because this supplier's products are not listed.
Maarten Swart, et al.,
bioRxiv - Immunology 2024
Quote:
... or 100 ng/ml N-palmitoyl-S-[2,3-bis(palmitoyloxy)-(2RS)-propyl]-[R]-cysteinyl-[S]-seryl-[S]-lysyl-[S]-lysyl-[S]-lysyl-[S]-lysine (Pam3CSK4) for 6 h and IL6 secretion measured by ELISA (R&D Systems).
-
No products found
because this supplier's products are not listed.
Elena Kreis, et al.,
bioRxiv - Plant Biology 2022
Quote:
... 250 μL 2× lysis buffer (20 mM Tris-HCl, 40 mM Na2EDTA, 1% (w/v) SDS) and 3 μL proteinase K (NEB: P8102S, 20 mg/mL) were added and incubated under agitation at 55°C for 2 h ...
-
No products found
because this supplier's products are not listed.
Mengfei Chen, et al.,
bioRxiv - Pathology 2022
Quote:
... Rabbit anti-SARS-CoV-2 Spike S (1:200, Sino Biological, 40150-R007), Goat anti-ACE2 (1:100 ...
-
No products found
because this supplier's products are not listed.
Lisanne de Vor, et al.,
bioRxiv - Microbiology 2021
Quote:
... were transfected with 1 μg DNA/ml cells in a 3:2 (LC:HC) ratio were transfected using polyethylenimine HCl MAX (Polysciences). After 4 to 5 days of expression ...
-
No products found
because this supplier's products are not listed.
Bolutife Fakoya, et al.,
bioRxiv - Microbiology 2021
Quote:
... Five female C57BL/6 dams with 2–3-day old litters (P2-3) (Charles River) were singly housed with their pups ...
-
No products found
because this supplier's products are not listed.
Astrid Kollewe, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 3 µg/ml Blasticidin S (InvivoGen) and 0.5 µg/ml Puromycin (Gibco ...
-
No products found
because this supplier's products are not listed.
Minh Ma, Saiaditya Badeti, Ke Geng, Dongfang Liu,
bioRxiv - Immunology 2020
Quote:
... Pseudotyped SARS-CoV-2-S (Genscript), full-length recombinant S protein (Acrobio systems) ...
-
No products found
because this supplier's products are not listed.
Shahanshah Khan, et al.,
bioRxiv - Immunology 2021
Quote:
... SARS-CoV-2 S (GTX632605, GeneTex) and ß-actin (A2228 ...
-
No products found
because this supplier's products are not listed.
Katalin Skrapits, et al.,
bioRxiv - Cell Biology 2021
Quote:
... biotin-conjugated secondary antibodies (Jackson ImmunoResearch; 1:500; 1h), ABC Elite reagent (1:1,000 ...
-
No products found
because this supplier's products are not listed.
Laura Alonso-Herranz, et al.,
bioRxiv - Immunology 2020
Quote:
... 1% P/S (Lonza), and 1 mM L-Gln (Lonza ...
-
No products found
because this supplier's products are not listed.
Fernando Y. Maeda, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Images were acquired at 1 frame/6-10 s and analyzed using Volocity (PerkinElmer) and NIH ImageJ ...
-
No products found
because this supplier's products are not listed.
Jessica Y Chen, et al.,
bioRxiv - Neuroscience 2020
Quote:
... mice used were 2-3-month-old C57BL/6 (Jackson Laboratories, Bar Harbor, ME, #000664) at the time of injury ...
-
No products found
because this supplier's products are not listed.
Wenchao Li, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... SUMO 2/3 (Proteintech, cat. 11251-1-ap, 1:1000 for WB); Ubiquitin (Proteintech ...
-
No products found
because this supplier's products are not listed.
Carmen Alicia Rivera Pérez, et al.,
bioRxiv - Microbiology 2021
Quote:
... and on cloudy days from 284 µmol m-2 s-1 to 154 µmol m-2 s-1 (Quantum/Radiometer/Photometer model 185B, LI-COR Inc., Lincoln, NE, USA). The cosms were regularly watered with demineralized water ...
-
No products found
because this supplier's products are not listed.
Vignesh Venkatakrishnan, et al.,
bioRxiv - Biochemistry 2020
Quote:
... disrupted with a tip sonicator at 1/3 power (8 MHz) for 3 × 30 seconds on ice and fractionated by ultracentrifugation (100’000 x g, 1H, Beckman TLA 120.2 rotor). The luminal fraction was concentrated to around 50 µL on a 3 kDa MWCO Amicon Ultra 0.5 mL centrifugal filter while membrane pellets were dissolved in 50 µL TBS with 2% SDS ...
-
No products found
because this supplier's products are not listed.
Vishantie Dostal, et al.,
bioRxiv - Physiology 2020
Quote:
... with 3 mM Tris(2-carboxyethyl)phosphine hydrochloride(TCEP-HCl) (Thermo Pierce) while shaking at 600 rpm in a thermomixer (Eppendorf). Samples were alkylated with 9 mM iodoacetamide (22 °C ...
-
No products found
because this supplier's products are not listed.
Haribaskar Ramachandran, et al.,
bioRxiv - Genomics 2021
Quote:
... cells were blocked with 3%BSA diluted in PBS for 1h at RT and then incubated with anti-CD-19-PE antibody (1:50) in 3% BSA-PBS (Miltenyi Biotec) for 1h at RT ...
-
No products found
because this supplier's products are not listed.
Sofia E. Luna, et al.,
bioRxiv - Genetics 2024
Quote:
2-3 days post-electroporation HSPCs were plated in SmartDish 6-well plates (cat.: 27370; STEMCELL Technologies, Vancouver, Canada) containing MethoCult H4434 Classic or MethoCult H4434 Classic without EPO (cat. ...
-
No products found
because this supplier's products are not listed.
Sergio P. Alpuche-Lazcano, et al.,
bioRxiv - Microbiology 2023
Quote:
The pEGFP-C1 plasmids including AFF4 3’UTR sites “1-2-3” were generated using pEGFP-C1 (Clontech). The following complementary oligonucleotides were annealed in respective pairs as above (1.25 µM each in 75 mM NaCl ...
-
No products found
because this supplier's products are not listed.
Aidan C. Daly, et al.,
bioRxiv - Systems Biology 2024
Quote:
... 5’ amine-modified DNA oligonucleotides (5’-[AmC6]dUdUdUdUd-[Illumina_adaptor]-[spatial barcode]-[UMI]-[20T]-VN ...
-
No products found
because this supplier's products are not listed.
Karthikeyan Thirugnanam, et al.,
bioRxiv - Cell Biology 2024
Quote:
... Smad 2/3 (Novus Biologicals, Cat# AF3797, 1:200 dilution), GAPDH (Santa Cruz ...
-
No products found
because this supplier's products are not listed.
Katerina Stepankova, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and anti-VGLUT1/2 (Synaptic Systems, #1235503, 1:800, 3 days). Goat anti-host antibodies of the respective primary antibodies conjugated with Alexa Fluor 405 ...
-
No products found
because this supplier's products are not listed.
Ruoyu Chai, et al.,
bioRxiv - Animal Behavior and Cognition 2021
Quote:
... The camera (Nikon, 25 frames s-1) is set up on the side of the observation area ...
-
No products found
because this supplier's products are not listed.
Zachary Beine, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The primary antibody (2-3% serum, 0.4% PBST, 1:500 Rockland 600-401-379) was applied and incubated for ninety minutes at room temperature ...
-
No products found
because this supplier's products are not listed.
Raianna F. Fantin, et al.,
bioRxiv - Immunology 2024
Quote:
... Plates were developed for 10 minutes at room temperature and then the reaction was stopped by adding 50 μL 3 M hydrochloric acid (HCl, Fisher) and plates were read using a Synergy 4 (BioTek) plate reader at an optical density (OD ...
-
No products found
because this supplier's products are not listed.
Stephen Abini-Agbomson, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Cells were incubated in blocking solution (3% BSA in TBS-Tween 0.1%) for 1h with primary antibody (anti-53BP1, Rabbit polyclonal, Bethyl #A300-272A) diluted in blocking solution overnight at 4°C ...