Ken Shirato, Jun Takanari, Takako Kizaki,bioRxiv - Pharmacology and Toxicology 2021
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... the cells were co-treated with the indicated concentrations of ETAS®50 or dextrin and 100 ng/mL of SARS-CoV-2 spike recombinant protein S1 subunit (Arigo Biolaboratories, Hsinchu City, Taiwan) for 1 to 24 h ...
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... The primary antibodies used (Bassoon, #ADI-VAM-PS003-F, Enzo Life Sciences GmbH, Lörrach, Germany; Homer1, #160003, Synaptic Systems) were diluted in the same buffer (lacking Triton X-100 ...
Denis Antonets, et al.,bioRxiv - Cell Biology 2019
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... TagRFP fragment without start codon was obtained by PCR with TagRFP-woATG-F 5’-GT CGGTACCGTGTCTAAGGGCGAAGAGCTG-3’ n BFPX3-R 5’-GCGCTTAAGTTAATTAAGCTTGTGCCCCA-3’ primers and pTagRFP-N vector (Evrogen) as a DNA source ...
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... the pellet was dissolved in Buffer F (50 mM Tris/HCl, 200 mM NaCl, pH 7.5) and lysed by cell disruption at 35 kPSI (Constant Systems). The lysate was cleared by ultracentrifugation (2 h at 35,000 rpm in a 45 Ti rotor ...
Neil J Rzechorzek, et al.,bioRxiv - Biochemistry 2020
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... HEK293-F cells were grown in 400 mL batches in 2 L non-baffled Erlenmeyer flasks (TriForest Enterprises Inc, #FPC2000S) to a cell density of ∼1×106 cells/mL.
Hyunsu Lee, Keith Bonin, Martin Guthold,bioRxiv - Biophysics 2020
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... A soft AFM probe (Biotool cell XXL, nominal spring constant k = 0.1 N/m, resonance frequency f = 50 kHz; Nanotools, Munich, Germany) was used for topographical imaging of live cells ...
Elizabeth V. K. Ledger, Andrew M. Edwards,bioRxiv - Microbiology 2023
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... or C3 was detected with a 1:1000 dilution of goat anti-human C3 F(ab’)2 labelled with FITC (Protos Immunoresearch). Antibody incubations were carried out statically for 1 h at room temperature in the dark ...