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Recombinant Mouse Regenerating Islet derived 3 Alpha His tagged
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Creative Biomart Sponsored
Recombinant Mouse Reg3a precursor extracellular domain (Met 1-Thr 457) (NP_035389.1), fused with...Cat# Reg3a-837M, 50.0 µg, USD $429.0 Ask
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Thermo Fisher
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019, published in Molecular Biology of the Cell doi: 10.1091/mbc.E19-07-0362Quote: Recombinant His-tagged EB1-GFP and His-tagged SEPT5 were transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 0.6-0.8 and induced with 0.2 mM IPTG for 16 h at 18°C for His-tagged-EB1-GFP or cultures were grown to OD600 of 0.4-0.6 and induced with 1 mM IPTG for 5 h at 23°C for His-tagged SEPT5 ... -
Millipore Sigma
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2019Quote: ... was incubated with recombinant His-tagged human HDAC6 protein (EMD Millipore) in 250 μl RB100 buffer (25 mM HEPES pH 7.5 ... -
Qiagen
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in Frontiers in Microbiology doi: 10.3389/fmicb.2019.02328Quote: ... Recombinant His-tagged GAPDH was purified using Ni-NTA Agarose (Qiagen, Valencia, USA) in native conditions according to the manufacturer’s recommendations ... -
Sino Biological
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: The following His-tagged recombinant proteins were purchased from Sino Biologicals: B.1.1.7 rS1-SARS-CoV-2 spike S1 [Δ 69-70 ... -
GE Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2017, published in Nature Microbiology doi: 10.1038/s41564-018-0203-xQuote: ... Purification of the His-tagged/GST-tagged recombinant proteins was performed as described by the manufacturer (Clontech/GE Healthcare). -
GenScript
No products found because this supplier's products are not listed.Cited in The C. difficile clnRAB operon initiates adaptations to the host environment in response to LL-37bioRxiv - Microbiology 2018, published in PLOS Pathogens doi: 10.1371/journal.ppat.1007153Quote: Recombinant N-terminally His-tagged ClnR was produced by GenScript (Piscataway, NJ). Gene transcription in the clnR mutant complemented with His-tagged ClnR confirmed the functionality of this protein (Table S12) ... -
R&D Systems
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019Quote: ... Recombinant Mouse Interferon Alpha-A (IFNα, R&D Systems, Inc. Minneapolis, MN) was administered either intratumorally or intramuscularly ... -
Peprotech
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2019Quote: ... 20ng/ml recombinant brain derived neurotrophic factor (BDNF, Peprotech), 20ng/ml recombinant glial-derived neurotrophic factor (GDNF ... -
abcam
No products found because this supplier's products are not listed.Cited in Distinct mechanisms of microRNA sorting into cancer cell-derived extracellular vesicle subtypesbioRxiv - Biochemistry 2019, published in eLife doi: 10.7554/eLife.47544Quote: ... anti-alpha integrin alpha 3 #ab190731 (Abcam, Cambridge, MA), anti-Mfg-e8 #MAB2767 (R&D systems ... -
Takara Bio
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2017, published in Nature Microbiology doi: 10.1038/s41564-018-0203-xQuote: ... Purification of the His-tagged/GST-tagged recombinant proteins was performed as described by the manufacturer (Clontech/GE Healthcare). -
BioLegend
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019, published in eLife doi: 10.7554/eLife.47528Quote: His-tagged recombinant soluble CD40L (sCD40L, BioLegend) was incubated with NTA-SUV or plain SUV ... -
Addgene
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2020Quote: ... and 6x-HIS-tagged-HOXB2 (Addgene 8522) were obtained from commercial vendors and cloned into pCMV6 vector and transfected into HEK cells ... -
New England Biolabs
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2019Quote: ... were coated to dry overnight at 37°C with 400 ng/well of recombinant His-tagged GT198 proteins together with 5 μg/well of purified BSA (NEB) in a volume of 50 μl ... -
Promega
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2018, published in Antimicrobial Agents and Chemotherapy doi: 10.1128/aac.00681-18Quote: ... a NADPH-regenerating system (Promega) was prepared in 0.1 M phosphate buffer (pH 7.4) ... -
Merck
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2018, published in Nature Communications doi: 10.1038/s41467-018-07251-5Quote: ... His-tagged proteins were immobilized on Ni-NTA (Merck Millipore), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ... -
Roche
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2019, published in mAbs doi: 10.1080/19420862.2020.1714371Quote: The recombinant biotinylated A33-His antigen was immobilized on streptavidin (StreptaWells, Roche) or avidin coated wells (Avidin ... -
Cell Signaling Technology
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018, published in mBio doi: 10.1128/mBio.01905-18Quote: ... His-tagged proteins were detected with mouse anti-His tag (27E8) antibody at a dilution of 1:1000 (Cell Signaling, Danvers, MA) and an anti-mouse IgG HRP linked antibody at a dilution of 1:2000 (Cell Signaling ... -
Origene Technologies
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2019, published in Nature Chemical Biology doi: 10.1038/s41589-019-0304-8Quote: Recombinant Flag-tagged RNF114 was used as bait to precipitate pure recombinant p21 (Origene Technologies Inc. ... -
Bio-Rad
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018, published in mBio doi: 10.1128/mBio.01006-18Quote: Recombinant His-tagged proteins were resolved by AnykDTM Mini-Protean TGX gels (Bio-Rad) and transferred to nylon-supported nitrocellulose ... -
Santa Cruz
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019, published in eLife doi: 10.7554/eLife.55957Quote: ... we used mouse antibodies against acetylated alpha-tubulin (Santa Cruz Biotechnologies, 1:160) and gamma-tubulin (Proteintech ... -
Agilent
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2018, published in Biochemical and Biophysical Research Communications doi: 10.1016/j.bbrc.2018.02.016Quote: ... The encoded N-terminal His-tagged fusion protein was overexpressed in Escherichia coli BL21 (DE3) Codon plus RIL (Stratagene) cells at 15 °C and purified by affinity chromatography on Ni-nitrilotriacetate resin (Qiagen) ... -
Alpha Diagnostic
No products found because this supplier's products are not listed.Cited in The plant pathogen Pectobacterium atrosepticum contains a functional formate hydrogenlyase-2 complexbioRxiv - Microbiology 2019, published in Molecular Microbiology doi: 10.1111/mmi.14370Quote: ... Nitrocellulose membranes were challenged with an anti-His-HRP antibody (Alpha Diagnostics) and a GeneGnome instrument (SynGene ... -
Lonza
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2017, published in Biology Open doi: 10.1242/bio.036806Quote: ... Cells were transfected with either human alpha V and beta 3 (CHO-K1) or alpha 5 and beta 1 (CHO-B2) using a Nucleofector II (Lonza) and Ingenio (Mirus ... -
Macherey-Nagel GmbH
No products found because this supplier's products are not listed.Cited in Allosteric deregulation of phenylalanine biosynthesis evolved with the emergence of vascular plantsbioRxiv - Plant Biology 2019Quote: ... Poly-His tagged recombinant proteins were purified using a nickel resin (Protino Ni-TED 2000 Packed Columns, Macherey-Nagel) and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns ... -
Corning
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2018Quote: ... bone-marrow derived macrophages (BMDMs) were washed 2-3 times with PBS (Corning) and then treated with tumor cell conditioned media or serum free media containing isolated tumor EVs supplemented with 20 ng/ml of mouse M-CSF ... -
Jackson ImmunoResearch
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018, published in eLife doi: 10.7554/eLife.42037Quote: ... and a donkey-derived HRP-conjugated anti-mouse (Jackson immunoresearch 715-035-151). Actin was detected using a monoclonal HRP-conjugated anti-actin antibody (Sigma A3854 ... -
Becton, Dickinson and Company
No products found because this supplier's products are not listed.bioRxiv - Systems Biology 2019Quote: ... Soluble mouse recombinant IL10 and IFNγ were procured from BD Biosciences (San Diego ... -
Enzo Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019, published in Nature Structural & Molecular Biology doi: 10.1038/s41594-020-0380-1Quote: ... 20 µM His-tagged ubiquitin (Enzo Life Sciences) in a reaction buffer of 50 mM HEPES pH 7.5 ... -
Proteintech
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2019, published in Journal of Biological Chemistry doi: 10.1074/jbc.RA119.011181Quote: ... His-tagged RmtC proteins were detected by immunoblotting with a rabbit anti-6×His antibody (α6×His; Proteintech; 10001-0-AP) overnight at 4 °C ... -
The Jackson Laboratory
No products found because this supplier's products are not listed.bioRxiv - Genomics 2019, published in Genome Biology doi: 10.1186/s13059-019-1699-yQuote: Mouse embryonic fibroblasts were derived from the C57BL/6J strain (The Jackson Laboratory 000664). HEK293T and mouse embryonic fibroblasts were cultured in Dulbecco’s Modified Eagle Medium (Gibco ... -
Boston Biochem
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2018Quote: Recombinant His-tagged ubiquitin and ubiquitin mutants were purchased from Boston Biochem, dissolved in LFB1/50 (40 mM Hepes/KOH pH 8.0 ... -
PerkinElmer
No products found because this supplier's products are not listed.bioRxiv - Physiology 2019Quote: ... Islets were seeded in a 384-well Optiplate (PerkinElmer) (4000 cells per well ... -
Illumina
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2017Quote: Gene expression was performed in islets from 131 donors using RNA-Seq (Illumina's TruSeq RNA Sample Preparation Kit and analyzed on the Illumina HiSeq2000 platform) ... -
Miltenyi Biotec
No products found because this supplier's products are not listed.bioRxiv - Plant Biology 2021Quote: ... To control for recombinant bacterial MCO expression anti-His-HRP (Miltenyi Biotec) was used (at a dilution of 1:10000 ... -
Bioline
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018Quote: ... All plasmids were propagated in DH5-alpha E.Coli (BIOLINE) and prepared for transfection using a Plasmid Maxi kit (QIAGEN). -
ImmunoTools
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... Recombinant human Interferon-alpha 2a (rh IFN-α2a) (10 µg/mL; ImmunoTools), or recombinant human IL-21 (40 ng/mL ... -
Calbiochem
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019, published in Molecular and Cellular Biology doi: 10.1128/MCB.00158-19Quote: ... and recombinant mouse M-CSF (Calbiochem) at a final concentration of 10ng/mL for seven days ... -
Novus Biologicals
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in Viruses doi: 10.3390/v11060563Quote: ... and mouse monoclonal anti-alpha-tubulin antibody (NB100-690; Novus Biologicals). Reovirus protein expression was assessed using a chicken polyclonal antiserum against μNS prepared against bacterially-expressed purified antigen by Covance ... -
Vector Labs
No products found because this supplier's products are not listed.bioRxiv - Genetics 2018Quote: ... and (d) FITC tagged anti-mouse antibody (1:200 dilution; Vector Labs). After each incubation ... -
Stemcell Technologies
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019, published in Journal of Thrombosis and Haemostasis doi: 10.1111/jth.15095Quote: ... 6 ng/mL recombinant mouse IL3 and 10 ng/mL recombinant human IL-6 (all from Stem Cell Technologies). On Day 0 ... -
NanoTemper
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in PLOS Pathogens doi: 10.1371/journal.ppat.1007848Quote: Three independent MST experiments were performed with His-tagged BspC labelled using the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies) according to manufacturer’s instructions ... -
PBL Assay Science
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020, published in Journal of Virology doi: 10.1128/JVI.01410-20Quote: ... Vero E6 cells were incubated with 1000 units/mL of recombinant type I IFN alpha (PBL Assay Sciences) 18 hours prior to infection (35) ... -
Tocris
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2019Quote: ... and alpha-bungarotoxin (TOCRIS) were injected in the center of the diencephalic ventricle in order to label CSF and paralyze the fish in a single injection ... -
LI-COR
No products found because this supplier's products are not listed.Cited in Secretion of an Argonaute protein by a parasitic nematode and the evolution of its siRNA guidesbioRxiv - Molecular Biology 2019, published in Nucleic Acids Research doi: 10.1093/nar/gkz142Quote: ... Quantification was carried by western blot analysis in comparison to known concentrations of recombinant full length exWAGO containing an N-terminal 3XFlag-His tag using Image Studio Lite (LI-COR) software ... -
Charles River Labs
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2020, published in Biomaterials doi: 10.1016/j.biomaterials.2020.120499Quote: Primary rat islets were isolated from 12-week Wistar Rats (Charles River) as previously described1,4 ... -
American Type Culture Collection
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019Quote: Mouse-derived C2C12 myoblasts (American Type Culture Collection, CRL-1772) were cultured in growth medium (GM) ... -
Biovision
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... 100 µl of mouse anti-His-tag antibody (1:1000, BioVision, USA) was incubated at 37□ for 2 h ... -
MP Biomedicals
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018, published in Applied and Environmental Microbiology doi: 10.1128/aem.02815-18Quote: ... alpha-chymotrypsin (MP Biomedicals, Illkirch ... -
Leica
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2020Quote: Islets were frozen with a Leica EM ICE high pressure freezer (Leica Microsystems, Germany) and kept in liquid nitrogen until freeze substitution ... -
ChromoTek
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2019Quote: ... Tagged proteins were immunoprecipitated with Myc-Trap_MA beads (ChromoTek) or anti-FLAG M2 Affinity gel (Sigma ...