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Recombinant Mouse Regenerating Islet derived 3 Alpha His tagged
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Creative Biomart Sponsored
Recombinant Mouse Reg3a precursor extracellular domain (Met 1-Thr 457) (NP_035389.1), fused with...Cat# Reg3a-837M, 50.0 µg, USD $429.0 Ask
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Thermo Fisher
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019, published in Molecular Biology of the Cell doi: 10.1091/mbc.E19-07-0362Quote: Recombinant His-tagged EB1-GFP and His-tagged SEPT5 were transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 0.6-0.8 and induced with 0.2 mM IPTG for 16 h at 18°C for His-tagged-EB1-GFP or cultures were grown to OD600 of 0.4-0.6 and induced with 1 mM IPTG for 5 h at 23°C for His-tagged SEPT5 ... -
Millipore Sigma
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ... -
Sino Biological
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... 1 µg recombinant His-tagged mouse IgSF8 (Sino Biological) was incubated in 1 ml binding buffer (10 mM HEPES pH 7.4 ... -
abcam
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2020Quote: ... Recombinant His-tagged human ATG4B (Abcam, ab188707) was pre-treated with 10 mM DTT for 15 mins ... -
Qiagen
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in Frontiers in Microbiology doi: 10.3389/fmicb.2019.02328Quote: ... Recombinant His-tagged GAPDH was purified using Ni-NTA Agarose (Qiagen, Valencia, USA) in native conditions according to the manufacturer’s recommendations ... -
R&D Systems
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2022Quote: ... Recombinant His-tagged NSP12 (R&D Systems, catalog # 10686-CV) was incubated with in vitro transcribed and biotinylated RNA in 20 mM Tris ... -
GE Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2017, published in Nature Microbiology doi: 10.1038/s41564-018-0203-xQuote: ... Purification of the His-tagged/GST-tagged recombinant proteins was performed as described by the manufacturer (Clontech/GE Healthcare). -
Peprotech
No products found because this supplier's products are not listed.bioRxiv - Genetics 2022Quote: ... 10ng/mL recombinant mouse interleukin 3 (rmIL-3, Peprotech) and 10ng/mL recombinant human interleukin 6 (rhIL-6 ... -
GenScript
No products found because this supplier's products are not listed.Cited in The C. difficile clnRAB operon initiates adaptations to the host environment in response to LL-37bioRxiv - Microbiology 2018, published in PLOS Pathogens doi: 10.1371/journal.ppat.1007153Quote: Recombinant N-terminally His-tagged ClnR was produced by GenScript (Piscataway, NJ). Gene transcription in the clnR mutant complemented with His-tagged ClnR confirmed the functionality of this protein (Table S12) ... -
BioLegend
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019, published in eLife doi: 10.7554/eLife.47528Quote: His-tagged recombinant soluble CD40L (sCD40L, BioLegend) was incubated with NTA-SUV or plain SUV ... -
Takara Bio
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2017, published in Nature Microbiology doi: 10.1038/s41564-018-0203-xQuote: ... Purification of the His-tagged/GST-tagged recombinant proteins was performed as described by the manufacturer (Clontech/GE Healthcare). -
Addgene
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2020Quote: 6×His-tagged VHH-mCherry (Addgene #109421) was transformed into BL21DE3 E.coli cells ... -
Merck
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ... -
Roche
No products found because this supplier's products are not listed.bioRxiv - Evolutionary Biology 2021Quote: ... HA-epitope tagged recombinant proteins were detected using a rat-derived monoclonal anti-HA antibody (dilution 1:200, Roche) followed by a secondary anti-rat antibody coupled to AlexaFluor 594 fluorophores (dilution 1:200 ... -
New England Biolabs
No products found because this supplier's products are not listed.Cited in Biochemical analysis of deacetylase activity of rice sirtuin OsSRT1, a class IV member in plantsbioRxiv - Plant Biology 2021Quote: Ni pull down experiments were performed using 3μl of His tagged OsSRT1 constructs (0.3μg/ul) and 1μg recombinant human Histone H3 (NEB) and mixed with 20 μl of Ni-NTA slurry (Qiagen) ... -
Santa Cruz
No products found because this supplier's products are not listed.bioRxiv - Plant Biology 2021Quote: ... His-tagged proteins were detected by a mouse anti-His antibody (sc-8036, 1:3000, Santa Cruz Biotechnology). GST-tagged proteins were detected by a mouse anti-GST antibody (sc-138 ... -
Promega
No products found because this supplier's products are not listed.Cited in Orchestration of Chlorophyll and Carotenoid Biosynthesis by ORANGE Family Proteins in PlantbioRxiv - Plant Biology 2022Quote: ... His-tagged and GST-tagged recombinant proteins were purified using Promega MagneHis™ Ni-Particles (Promega Cat#V854A) and MagneGST™ Glutathione Particles (Promega Cat# V861A ... -
Bio-Rad
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018, published in mBio doi: 10.1128/mBio.01006-18Quote: Recombinant His-tagged proteins were resolved by AnykDTM Mini-Protean TGX gels (Bio-Rad) and transferred to nylon-supported nitrocellulose ... -
Origene Technologies
No products found because this supplier's products are not listed.Cited in Destabilization of β-cell FIT2 by saturated fatty acids contribute to ER stress and diabetesbioRxiv - Biochemistry 2021Quote: ... transiently co-transfected with pcDNA3.1-FIT2/V5-His and GFP-tagged MARCH6 (BC059190) Mouse Tagged ORF Clone (Origene), were pre-treated with 10 µM MG132 (Cell Signaling Technology ... -
Agilent
No products found because this supplier's products are not listed.Cited in Integrase-RNA interactions underscore the critical role of integrase in HIV-1 virion morphogenesisbioRxiv - Microbiology 2019Quote: All of the mutations were introduced into a plasmid backbone expressing His6 tagged pNL4-3-derived IN by QuikChange site directed mutagenesis kit (Agilent) [60] ... -
Acrobiosystems
No products found because this supplier's products are not listed.Cited in SARS-CoV-2 Beta variant infection elicits potent lineage-specific and cross-reactive antibodiesbioRxiv - Immunology 2021Quote: His-tagged recombinant RBD Beta protein was produced in HEK cells (ACROBiosystems, SPD-C52Hp) and covalently labeled using CruzFluor488 (Santa Cruz Biotechnology ... -
Stemcell Technologies
No products found because this supplier's products are not listed.Cited in Human brain organoid networksbioRxiv - Neuroscience 2021Quote: ... 20 ng/ml Recombinant Human Brain-Derived Neurotrophic Factor (StemCell Technologies), 20 ng/ml Recombinant Human Glial-Derived Neurotrophic Factor (StemCell Technologies) ... -
Corning
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2022Quote: ... 3% HI-FBS (Corning), 1% Penicillin-Streptomycin (Sigma) ... -
Cell Signaling Technology
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018, published in mBio doi: 10.1128/mBio.01905-18Quote: ... His-tagged proteins were detected with mouse anti-His tag (27E8) antibody at a dilution of 1:1000 (Cell Signaling, Danvers, MA) and an anti-mouse IgG HRP linked antibody at a dilution of 1:2000 (Cell Signaling ... -
Boston Biochem
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2018Quote: Recombinant His-tagged ubiquitin and ubiquitin mutants were purchased from Boston Biochem, dissolved in LFB1/50 (40 mM Hepes/KOH pH 8.0 ... -
Proteintech
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2019, published in Journal of Biological Chemistry doi: 10.1074/jbc.RA119.011181Quote: ... His-tagged RmtC proteins were detected by immunoblotting with a rabbit anti-6×His antibody (α6×His; Proteintech; 10001-0-AP) overnight at 4 °C ... -
Alpha Diagnostic
No products found because this supplier's products are not listed.Cited in The plant pathogen Pectobacterium atrosepticum contains a functional formate hydrogenlyase-2 complexbioRxiv - Microbiology 2019, published in Molecular Microbiology doi: 10.1111/mmi.14370Quote: ... Nitrocellulose membranes were challenged with an anti-His-HRP antibody (Alpha Diagnostics) and a GeneGnome instrument (SynGene ... -
RayBiotech
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... recombinant human His-tagged ACE-2 protein (RayBiotech) was incubated on cells before adding any antibodies ... -
Macherey-Nagel GmbH
No products found because this supplier's products are not listed.Cited in Allosteric deregulation of phenylalanine biosynthesis evolved with the emergence of vascular plantsbioRxiv - Plant Biology 2019Quote: ... Poly-His tagged recombinant proteins were purified using a nickel resin (Protino Ni-TED 2000 Packed Columns, Macherey-Nagel) and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns ... -
VWR
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2021Quote: ... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ... -
NanoTemper
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2021Quote: Recombinant His-tagged proteins were labeled using the Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper, MO-L008). In all experiments ... -
Calbiochem
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... 50 μL mouse monoclonal anti-6X His tagged primary antibody (Calbiochem) was added at a dilution of 1:150 in 0.5 % BSA in PBST in the respective wells and incubated for 2 hours without shaking at room temperature ... -
PerkinElmer
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... Streptavidin-coated Alpha donor beads or anti-His-conjugated AlphaLISA acceptor beads (PerkinElmer) were used at a final concentration of 10 μg/mL per well ... -
Becton, Dickinson and Company
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... single islet cells were stained with TMRE and FluoZin-3 and sorted with a FACSAria II (BD). In some experiments intracellular staining with anti-insulin (R&D Systems ... -
Enzo Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019, published in Nature Structural & Molecular Biology doi: 10.1038/s41594-020-0380-1Quote: ... 20 µM His-tagged ubiquitin (Enzo Life Sciences) in a reaction buffer of 50 mM HEPES pH 7.5 ... -
The Jackson Laboratory
No products found because this supplier's products are not listed.Cited in The Ndc80-Cdt1-Ska1 complex constitute a minimal processive kinetochore-microtubule coupling unitbioRxiv - Cell Biology 2022Quote: ... HRP-tagged mouse/rabbit antibodies (Jackson laboratories) were used at 1:10,000 dilution to detect the bait-prey complex and blot was developed by Super signal west pico chemiluminescent substrate (Thermofisher). -
Sartorius
No products found because this supplier's products are not listed.bioRxiv - Synthetic Biology 2022Quote: ... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ... -
Novus Biologicals
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... mouse anti-alpha-tubulin (Novus biological DM1A), Rabbit anti-calnexin (abcam 22595) ... -
Lonza
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2017, published in Biology Open doi: 10.1242/bio.036806Quote: ... Cells were transfected with either human alpha V and beta 3 (CHO-K1) or alpha 5 and beta 1 (CHO-B2) using a Nucleofector II (Lonza) and Ingenio (Mirus ... -
Jackson ImmunoResearch
No products found because this supplier's products are not listed.Cited in Development of a monoclonal antibody to a vibriophage as a proxy for Vibrio cholerae detectionbioRxiv - Immunology 2022Quote: ... Horseradish peroxidase-tagged goat anti-mouse IgG (Jackson ImmunoResearch) was added at 1:1000 dilution to detect antigen bound IgG mAbs ... -
Miltenyi Biotec
No products found because this supplier's products are not listed.bioRxiv - Plant Biology 2021Quote: ... To control for recombinant bacterial MCO expression anti-His-HRP (Miltenyi Biotec) was used (at a dilution of 1:10000 ... -
PBL Assay Science
No products found because this supplier's products are not listed.bioRxiv - Immunology 2022Quote: ... Interferon alpha was measured by ELISA (Mouse IFN Alpha All Subtype ELISA Kit, High Sensitivity, PBL Assay Science). Blood was left at room temperature for 20 min and after centrifugation serum was frozen at -20°C until further analysis ... -
Abclonal Science
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2021Quote: ... platelet-derived growth factor receptor alpha (pdgfr-α) (Abclonal), CAPG (ProteinTech) ... -
Active Motif
No products found because this supplier's products are not listed.Cited in G9a/GLP methyltransferases inhibit autophagy by methylation-mediated ATG12 protein degradationbioRxiv - Molecular Biology 2021Quote: Recombinant Flag-tagged human G9a (Active Motif, 31410), human calpain 1 (Novus ... -
Illumina
No products found because this supplier's products are not listed.bioRxiv - Genomics 2021Quote: ... and (3) 134 Gb (~100× depth) chromosome conformation capture sequencing (Hi-C) data (sequenced by Illumina platform). -
BPS Bioscience
No products found because this supplier's products are not listed.Cited in Suramin potently inhibits binding of the mammalian high mobility group protein AT-hook 2 to DNAbioRxiv - Biochemistry 2019Quote: ... His-tagged BRD4-BD1 was purchased from BPS Bioscience (#31042). Pre-acetylated Biotin-Histone 4 Peptide was purchased from AnaSpec (#64989-025) ... -
ImmunoTools
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... Recombinant human Interferon-alpha 2a (rh IFN-α2a) (10 µg/mL; ImmunoTools), or recombinant human IL-21 (40 ng/mL ... -
Vector Labs
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... Cytiva) for His-tagged monomers and gravity flow columns filled with Agarose bound Galnthus Nivalis Lectin beads (Vector Labs) for nanoparticles ... -
Invivogen
No products found because this supplier's products are not listed.bioRxiv - Immunology 2022Quote: ... cDNA encoding His-tagged Spike and RBD variants of SARS-CoV-2 were sub-cloned into pFUSE2ss-CLIg-hk (InvivoGen). The vectors were transfected as described for above the ACE2-albumin fusion protein and secreted His-tagged proteins were purified on HisTrap™ HP 1 mL columns (Cytiva) ... -
Abnova
No products found because this supplier's products are not listed.Cited in Stress-induced tyrosine phosphorylation of RtcB modulates IRE1 activity and signaling outputsbioRxiv - Biochemistry 2022Quote: ... from Carna Biosciences as the tyrosine kinase and 1 μg of human recombinant GST-tagged RtcB (GST-C22orf28: 81.6 kDa) (P01) from Abnova as the substrate ...