-
No products found
because this supplier's products are not listed.
Jun Weng, et al.,
bioRxiv - Biochemistry 2021
Quote:
Binding of nucleobases was performed by means of the scintillation proximity assay (SPA) using His-tagged PurTCp in conjunction with Copper HIS-Tag YSI beads (Perkin Elmer, RPNQ0096). 250 ng of purified PurTCp were immobilized on 125 μg SPA beads per 100-μL assay in 200 mM Tris/Mes at the indicated pH ...
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No products found
because this supplier's products are not listed.
Kylie M. Konrath, et al.,
bioRxiv - Immunology 2021
Quote:
... Cytiva) for His-tagged monomers and gravity flow columns filled with Agarose bound Galnthus Nivalis Lectin beads (Vector Labs) for nanoparticles ...
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No products found
because this supplier's products are not listed.
Michael G. Spelios, et al.,
bioRxiv - Immunology 2021
Quote:
... purified His-tagged ACE2 (EpiGentek) was added at a concentration of 100 ng/well (prepared with PBS ...
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No products found
because this supplier's products are not listed.
Madison A. DeWinter, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... his-tagged extracellular domain of the growth hormone receptor (GHR, BioVision 7477-10) was immobilized on the Ni-NTA sensor tip by dipping the tips in a 2.5 μg/ml solution of GHR for 400 s ...
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No products found
because this supplier's products are not listed.
Miao-Hsi Hsieh, et al.,
bioRxiv - Microbiology 2020
Quote:
... SARS-CoV-2 S1 protein containing a C-terminal His-tag (Acro; S1N-C52H3) (5 μg/ml) was tagged with anti-His antibody (Genetex; GT395) (1:100 ...
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No products found
because this supplier's products are not listed.
Matthew D. Cain, et al.,
bioRxiv - Immunology 2023
Quote:
Recombinant mouse IFNα (PBL Assay Science, 12100-1) was administered intranasally ...
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No products found
because this supplier's products are not listed.
Andrew G. Diamos, et al.,
bioRxiv - Immunology 2020
Quote:
... Plant-expressed 6-His tagged ZE3 at 50 ng/well was bound to 96-well high-binding polystyrene plates (Corning Inc, Corning, NY, USA), and the plates were blocked with 5% nonfat dry milk in PBST ...
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No products found
because this supplier's products are not listed.
Teddy Kamata, Chun-Song Yang, Bryce M. Paschal,
bioRxiv - Biochemistry 2021
Quote:
... recombinant GST-tagged tandem AF1521 was fluorescently labeled using IRDye® 800CW Protein Labeling Kit (LI-COR Biosciences, Lincoln, NE, USA). Prepared samples were run on SDS-PAGE ...
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No products found
because this supplier's products are not listed.
Alexandre B. Leitão, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
... Sensifast Hi-Rox SyBr kit (Bioline) was used to perform the RT-qPCR on a StepOnePlus system (ThermoFisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Hristina Vasileva, et al.,
bioRxiv - Immunology 2023
Quote:
... the SC and V2 domains were cloned into the pET15b expression vector as dual His (N-terminal) and Myc (C-terminal) tagged recombinant constructs (Biomatik, USA). A duplicate set of expression constructs were also cloned into the pGEX-4T-1 expression vector as single-tagged GST (N-terminal ...
-
No products found
because this supplier's products are not listed.
Amit Rahi, et al.,
bioRxiv - Cell Biology 2022
Quote:
... HRP-tagged mouse/rabbit antibodies (Jackson laboratories) were used at 1:10,000 dilution to detect the bait-prey complex and blot was developed by Super signal west pico chemiluminescent substrate (Thermofisher).
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No products found
because this supplier's products are not listed.
Young Rock Chung, et al.,
bioRxiv - Immunology 2023
Quote:
... Goat anti-mouse IgG antibody tagged with streptavidin-HRP (Southern Biotech, 7105-05) was diluted 1:400 in blocking buffer and incubated for 1 hr at room temperature ...
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No products found
because this supplier's products are not listed.
Meenakshi Basu Shrivastava, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 500 ng human recombinant His-tagged ubiquitin-conjugating enzyme (E2) Ube2d3 (from BIOMOL International, #U0880), in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Benjamin N. Bell, et al.,
bioRxiv - Immunology 2021
Quote:
His-tagged SARS-CoV-2 RBD (300 nM) was loaded on His1K biosensors (Pall ForteBio) to a load threshold of 0.4-0.6 nm ...
-
No products found
because this supplier's products are not listed.
Kerstin Voelkl, et al.,
bioRxiv - Neuroscience 2023
Quote:
... mouse anti-His (Dianova, DIA-900-100, 1:1,000), chicken anti-EGFP (Invitrogen ...
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No products found
because this supplier's products are not listed.
Ivan Martinez-Valbuena, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Recombinant α-synuclein (rPeptide) was thawed from −80 ◦C storage ...
-
No products found
because this supplier's products are not listed.
Alanna B. Chan, et al.,
bioRxiv - Cell Biology 2021
Quote:
His-tagged mouse CRY2 D325H PHR domain (residues 1-512) was expressed in Sf9 suspension insect cells (Expression Systems, Davis, CA) infected with a P3 baculovirus at 1.2 × 106 cells per milliliter and grown for 72 hours at 27°C with gentle shaking ...
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No products found
because this supplier's products are not listed.
Joanna Young, et al.,
bioRxiv - Microbiology 2020
Quote:
... Recombinant His-SFP1 was used to immunize female New Zealand white rabbits (Covalab) for generation of polyclonal antibodies ...
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No products found
because this supplier's products are not listed.
Sandra D. Chanez-Paredes, et al.,
bioRxiv - Cell Biology 2023
Quote:
... mouse anti-6-His (Bio X Cell, AB_2687790) was used for western blot (3 µg/ml) ...
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No products found
because this supplier's products are not listed.
Kylie M. Konrath, et al.,
bioRxiv - Immunology 2021
Quote:
... goat anti-mouse IgG h+l HRP-tagged antibody (Bethyl Laboratories) diluted 1:20000 ...
-
No products found
because this supplier's products are not listed.
Trung The Tran, et al.,
bioRxiv - Immunology 2022
Quote:
... cDNA encoding His-tagged Spike and RBD variants of SARS-CoV-2 were sub-cloned into pFUSE2ss-CLIg-hk (InvivoGen). The vectors were transfected as described for above the ACE2-albumin fusion protein and secreted His-tagged proteins were purified on HisTrap™ HP 1 mL columns (Cytiva) ...
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No products found
because this supplier's products are not listed.
Matthew J. Burke, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Single-cell clones were then assessed for binding to His-tagged SARS-CoV-2 Spike using a Cytoflex S (Beckman) cell analyser ...
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No products found
because this supplier's products are not listed.
Ling He, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The pull-down protein samples were then subjected to western blotting with the whole cell lysate and His-tagged Rac1 protein (#RC01, Cytoskeleton) serving as the controls.
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No products found
because this supplier's products are not listed.
Keval Bollavaram, et al.,
bioRxiv - Bioengineering 2021
Quote:
... and donkey anti-mouse secondary antibodies tagged with an infrared fluorophore (Rockland Immunochemicals). Membranes were imaged with a LI-COR Odyssey scanner.
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No products found
because this supplier's products are not listed.
Hayden T. Pacl, et al.,
bioRxiv - Microbiology 2021
Quote:
... was treated with 300 nM of purified recombinant SARS-Cov2 NSP13 with an N-terminal His-tag (Cayman Chemicals #30589) in the presence of 50 mM Tris pH-7.4 ...
-
No products found
because this supplier's products are not listed.
Joseph Atherton, et al.,
bioRxiv - Biochemistry 2020
Quote:
... HA-tagged constructs and bioGFP-tagged constructs using MaxPEI (Polysciences) in a ratio of 3/1 PEI/DNA ...
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No products found
because this supplier's products are not listed.
Gongshi Bai, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... FAM-tagged TelG probe (PNA Bio F1005) was diluted (1:100 ...
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No products found
because this supplier's products are not listed.
Sarah N. Zvornicanin, et al.,
bioRxiv - Biochemistry 2023
Quote:
HI-Control™ BL21(DE3) (Lucigen) cells were transformed with plasmid and single colonies were picked to start overnight cultures in LB medium supplemented with 50 μg/mL kanamycin at 37 °C ...
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No products found
because this supplier's products are not listed.
Maria Steene Eriksen, et al.,
bioRxiv - Biochemistry 2019
Quote:
Fluorescently tagged Arc and StrepII tagged Arc were coexpressed in HEK293 cells and purified using Strep-Tactin Sepharose (IBA Lifesciences). Formation of complexes between the indicated Arc constructs was analyzed by immunoblotting following SDS electrophoresis ...
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No products found
because this supplier's products are not listed.
Ines A Cadena, et al.,
bioRxiv - Bioengineering 2024
Quote:
... and recombinant human insulin 0.5%) (Lonza). Human cervical cancer cell line SiHa from ATCC (ATCC® HTB-35™ ...
-
No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... 5 μg of His-tagged H2A:H2B or His-H3:Flag-H4 (Diagenode) and 2 μg of Flag-hSpt16 were added to 250 μl of binding buffer (50 mM Tris-HCl pH 7.4 ...
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No products found
because this supplier's products are not listed.
Michael P. Doyle, et al.,
bioRxiv - Immunology 2022
Quote:
... his-tagged YFV E protein (Meridian Life Science) was associated to the tips at 5 μg/mL for 60 sec ...
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No products found
because this supplier's products are not listed.
Tomohisa Sebastian Tanabe, et al.,
bioRxiv - Biochemistry 2023
Quote:
... His-tagged and Strep-tagged proteins were purified with Ni-NTA Agarose (Jena Bioscience) (except for AqTuA that was purified with a HiScreen Ni FF column (Cytiva) ...
-
No products found
because this supplier's products are not listed.
Mariska S. Simpson, et al.,
bioRxiv - Cell Biology 2023
Quote:
... His-tagged proteins were affinity purified by binding to cobalt resin (GoldBio) equilibrated in 1X PBS ...
-
No products found
because this supplier's products are not listed.
Huilei Wang, et al.,
bioRxiv - Biochemistry 2021
Quote:
... An adenovirus used to overexpress C-terminal His-tagged LOXL2 was purchased from Vector Biolabs. Vitamin E (α-Tocopherol ...
-
No products found
because this supplier's products are not listed.
Priyadharshini Devarajan, et al.,
bioRxiv - Immunology 2022
Quote:
... and recombinant Mouse IL-15 (Tonbo Biosciences) were suspended and mixed in PBS ...
-
No products found
because this supplier's products are not listed.
Georgia Balsevich, et al.,
bioRxiv - Neuroscience 2022
Quote:
... recombinant mouse leptin (Cedarlane Laboratories Ltd, CLCYT351-2), and Compound C (Selleck Chemicals ...
-
No products found
because this supplier's products are not listed.
Chia-Yang Chen, et al.,
bioRxiv - Plant Biology 2024
Quote:
... recombinant mononucleosomes (EpiCypher) as substrates ...
-
No products found
because this supplier's products are not listed.
Saurabh Srivastava, et al.,
bioRxiv - Molecular Biology 2021
Quote:
Biotinylation of SNAP tagged proteins and Avi-tagged proteins were performed as suggested by manufactures (NEB, Avidity). Direct biotinylation of proteins for example-YenB ...
-
No products found
because this supplier's products are not listed.
Máté Fischer, et al.,
bioRxiv - Bioengineering 2019
Quote:
... nanoparticle-tagged FITC and fluorescence-tagged immune cell populations were imaged using a Leica TCS SP8 Confocal Microscope (Leica Microsystems). DAPI ...
-
No products found
because this supplier's products are not listed.
Weisong Zhao, et al.,
bioRxiv - Biophysics 2022
Quote:
... equipped with an objective (100×/1.7 HI oil, APON, Olympus)and a sCMOS (Flash 4.0 V3 ...
-
No products found
because this supplier's products are not listed.
Tridib Ganguly, et al.,
bioRxiv - Microbiology 2022
Quote:
... Recombinant His-tagged was ZccR overexpressed by addition of 0.2 mM isopropyl-β-D-1- thiogalactopyranoside (IPTG) (Teknova) and the culture was incubated for additional 3 h ...
-
No products found
because this supplier's products are not listed.
Shuangshuo Jia, et al.,
bioRxiv - Cell Biology 2023
Quote:
... recombinant His-S ANXA1 protein (G15574, LifeSpan BioSciences, Seattle, WA, USA) was injected into the intra-articular cavity of SD rats four times weekly (1.3μg/50μL ...
-
No products found
because this supplier's products are not listed.
Yu Rebecca Miao, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... and mouse anti-HIS Hilyte Fluor 488 (Anaspec).
-
No products found
because this supplier's products are not listed.
Hourinaz Behesti, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... 100 ng/ml recombinant human/mouse FGF8b (Tocris) between days 1-11 (range tested ...
-
No products found
because this supplier's products are not listed.
Carolin Fritz, et al.,
bioRxiv - Cell Biology 2024
Quote:
... His (MP Biomedicals) plates ...
-
No products found
because this supplier's products are not listed.
Yen-Der Li, et al.,
bioRxiv - Biochemistry 2023
Quote:
... transfected with 9 μg HA-tagged and 9 μg Flag-tagged constructs using TransIT-LT1 transfection reagents (Mirus). The transfected cells were cultured for another two days ...
-
No products found
because this supplier's products are not listed.
Natalia Sanchez de Groot, et al.,
bioRxiv - Systems Biology 2019
Quote:
... SD -His with uracil and 5FOA (Zymo Research) was used to analyze the phase separation effects of a toxic Ura3p activity.
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No products found
Katharina M. Eyme, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... human recombinant EGF (20 ng/mL; ABM), and human recombinant bFGF-2 (10ng/mL ...
-
No products found
because this supplier's products are not listed.
Chungwon Park, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Neurons were pre-incubated with 3 nM of CypHer5E-tagged anti-VGAT antibodies (131 103CpH, Synaptic Systems, Germany) in the culture medium and incubated in the 5% CO2 incubator for 2 h at 37°C to specifically label inhibitory synaptic vesicles in presynaptic terminals ...