-
No products found
because this supplier's products are not listed.
Chien-Wei Wang, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Recombinant His-tagged streptavidin (Fitzgerald Industries, Acton, MA) at a concentration of 0.75 μM was used as a negative control molecule ...
-
No products found
because this supplier's products are not listed.
Hristina Vasileva, et al.,
bioRxiv - Immunology 2023
Quote:
... the SC and V2 domains were cloned into the pET15b expression vector as dual His (N-terminal) and Myc (C-terminal) tagged recombinant constructs (Biomatik, USA). A duplicate set of expression constructs were also cloned into the pGEX-4T-1 expression vector as single-tagged GST (N-terminal ...
-
No products found
because this supplier's products are not listed.
Danila Boytsov, et al.,
bioRxiv - Biophysics 2022
Quote:
... the FCS unit (Confocor3, Carl Zeiss, Jena, Germany) of a commercial laser scanning microscope (LSM 510 ...
-
No products found
because this supplier's products are not listed.
Oskar Staufer, et al.,
bioRxiv - Immunology 2022
Quote:
... beads were incubated with varying amount of the recombinant his-tagged proteins and corresponding MFIs were compared to reference beads of know AlexFluor molecule densities (Bangs Laboratories, IN).
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No products found
because this supplier's products are not listed.
Michael P. Doyle, et al.,
bioRxiv - Immunology 2022
Quote:
... his-tagged YFV E protein (Meridian Life Science) was associated to the tips at 5 μg/mL for 60 sec ...
-
No products found
because this supplier's products are not listed.
Meenakshi Basu Shrivastava, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 500 ng human recombinant His-tagged ubiquitin-conjugating enzyme (E2) Ube2d3 (from BIOMOL International, #U0880), in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich ...
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No products found
Daniel M. Sapozhnikov, Moshe Szyf,
bioRxiv - Molecular Biology 2020
Quote:
... and dCas9 recombinant protein (Applied Biological Materials Inc.). After 10 minutes at room temperature ...
-
No products found
because this supplier's products are not listed.
Denise Jahn, et al.,
bioRxiv - Cell Biology 2023
Quote:
... recombinant mouse Cgrp (Bachem #H-2265, 10-7M).
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No products found
because this supplier's products are not listed.
Albert Herms, et al.,
bioRxiv - Cell Biology 2023
Quote:
... recombinant Wnt3a protein (100 ng/mL, AMSBIO, AMS.rmW3aH-010), recombinant R-Spondin 1 protein (500 ng/m ...
-
No products found
because this supplier's products are not listed.
Kathryn L. Wofford, et al.,
bioRxiv - Bioengineering 2019
Quote:
... either human recombinant myelin basic protein (Alfa Aesar, cat. #AAJ66051LB0) fluorescently labeled with Vybrant DiD (Molecular Probes ...
-
No products found
because this supplier's products are not listed.
Arun Upadhyay, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 10 nM recombinant MT3 protein (Boster Bio Cat no. PROTP25713) was added at a in additional wells ...
-
No products found
because this supplier's products are not listed.
Robert T.P. Williams, et al.,
bioRxiv - Genomics 2023
Quote:
... FLAPY probes were labeled with Cal Fluor 610 and FLAP X probes were labeled with Quasar 670 (Biosearch Technologies, BNS-5082 and FC-1065, respectively). All samples were imaged on a DeltaVision Elite inverted microscope described above ...
-
No products found
because this supplier's products are not listed.
Shuangshuo Jia, et al.,
bioRxiv - Cell Biology 2023
Quote:
... recombinant His-S ANXA1 protein (G15574, LifeSpan BioSciences, Seattle, WA, USA) was injected into the intra-articular cavity of SD rats four times weekly (1.3μg/50μL ...
-
No products found
because this supplier's products are not listed.
Yoji Tsugawa, et al.,
bioRxiv - Microbiology 2024
Quote:
... The recombinant proteins were purified from supernatants by NGL COVID-19 Spike Protein Affinity Resin (Repligen) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Temitayo T. Bamgbose, et al.,
bioRxiv - Immunology 2023
Quote:
... and treated with recombinant mouse IL-4 (Leinco technologies, Inc. I-207) at a 40ng/ml concentration for 6 hours ...
-
No products found
because this supplier's products are not listed.
Stacia M. Nicholson, Francis A.X. Schanne,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... Fc receptors were blocked for 30 min with Protein A (MP Biomedicals, OH) in Stain Buffer (554656 ...
-
No products found
because this supplier's products are not listed.
Luisa Diomede, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... FLAG-tagged ACE2 (AdipoGen) was captured on the chip by a previously immobilized anti-FLAG antibody (Merck Life Science S.r.l) ...
-
No products found
because this supplier's products are not listed.
Yicong Wu, et al.,
bioRxiv - Bioengineering 2021
Quote:
... DNA was labeled by 5 μg/mL of mouse-α-DNA (Progen, 61014) and 1 μg/mL of α-mouse-JF549 (Novus ...
-
No products found
because this supplier's products are not listed.
Francesca Curreli, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... Various concentrations of biotinylated recombinant SARS-CoV-2 Spike RBD protein (Bioss Antibodies, Woburn, MA) dissolved in water were manually printed onto the Chip with Biodo bioprinting at 40% humidity via biotin-avidin conjugation ...
-
No products found
because this supplier's products are not listed.
Manoj K Singh, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... The proteins were labeled using 8plex iTRAQ reagent as per the manufacturer’s protocol (AB Sciex). The peptides were separated on Eksigent nano-LC (Ultra 2D ...
-
No products found
because this supplier's products are not listed.
Yue Zhu, Seyit Yuzuak, Xiaoyan Sun, De-Yu Xie,
bioRxiv - Plant Biology 2023
Quote:
The purification of recombinant protein was carried out on a Ni-NTA column (G-Biosciences, USA). One ml of Ni-NTA Sefinose resin was loaded onto a plastic column ...
-
No products found
because this supplier's products are not listed.
Ella F.J. Halcrow, et al.,
bioRxiv - Cell Biology 2022
Quote:
... HeLa cells expressing the different GFP-tagged proteins were plated on an open μ-Slide with 8 wells (Ibidi, 80826) in complete medium containing 0.5 μM SiR-DNA dye (SpiroChrome) ...
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No products found
because this supplier's products are not listed.
Alanna B. Chan, et al.,
bioRxiv - Cell Biology 2021
Quote:
His-tagged mouse CRY2 D325H PHR domain (residues 1-512) was expressed in Sf9 suspension insect cells (Expression Systems, Davis, CA) infected with a P3 baculovirus at 1.2 × 106 cells per milliliter and grown for 72 hours at 27°C with gentle shaking ...
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No products found
because this supplier's products are not listed.
Miriam R. Fein, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Fc receptor blocker (Innovex Biosciences), and finally avidin/biotin blocking buffer (Vector Laboratories ...
-
No products found
because this supplier's products are not listed.
Irina Shlosman, et al.,
bioRxiv - Biophysics 2022
Quote:
Purified proteins were labeled with 5-10 molar excess of sulfo-Cy3-maleimide and sulfo-Cy3-maleimide (Lumiprobe) in 20 mM Hepes pH 7.5 ...
-
No products found
because this supplier's products are not listed.
Neil G. Rumachik, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... FRG mice were maintained on irradiated high-fat low-protein mouse chow (Envigo Teklad Global 16% Protein Cat#2916) ad libitum to decrease flux through the tyrosine pathway ...
-
No products found
because this supplier's products are not listed.
Junjie Xu, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The probe was tagged to opal 570 dye from Akoya Biosciences (Marlborough ...
-
No products found
because this supplier's products are not listed.
Kazuki Hattori, et al.,
bioRxiv - Bioengineering 2022
Quote:
... The device was presoaked in FC-40 (3M) for more than 30 min and placed in a stage top incubator (STXG-WSKMX ...
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No products found
because this supplier's products are not listed.
Wanqiu Ding, et al.,
bioRxiv - Evolutionary Biology 2023
Quote:
Total protein was extracted from the mouse brain using RIPA lysis buffer (Solarbio R0020) supplemented with 1 mM PMSF and complete protease inhibitor cocktail ...
-
No products found
because this supplier's products are not listed.
Elsa Leitão, et al.,
bioRxiv - Genetics 2020
Quote:
... Tagged DNA was purified with the DNA Clean & Concentrator-5 kit (Zymo Research) eluting with 14 µl EB buffer (Qiagen) ...
-
No products found
because this supplier's products are not listed.
Tyler B. Waltz, et al.,
bioRxiv - Neuroscience 2023
Quote:
Recombinant rat protein p11 (S100A10 Recombinant Protein, Aviva Systems Biology, OPCD06771) was dissolved in distilled water to obtain a final concentration of 100ug/mL and stored at -80C for less than one month ...
-
No products found
because this supplier's products are not listed.
Yanzhou Zhang, et al.,
bioRxiv - Cell Biology 2021
Quote:
His-tagged or His-sumo-tagged protein was purified using NiNTA beads (Anatrace, Cat. No. SUPER-NINTA25). Briefly ...
-
No products found
because this supplier's products are not listed.
Jia Wang, Xincheng Zhong, Hang Yin,
bioRxiv - Immunology 2023
Quote:
Recombinant TRAF6(50-350) and other variants were labeled with Cyanine 3 (AAT Bioquest), UBE2N ...
-
No products found
because this supplier's products are not listed.
Yi-Nan Zhang, et al.,
bioRxiv - Immunology 2021
Quote:
... Recombinant mouse Flt3 ligand (Flt3L) and mouse SCF were purchased from Shenandoah Biotech (Warwick, PA). Cells were stained with appropriate concentrations of mAbs ...
-
No products found
because this supplier's products are not listed.
Francois Chesnais, et al.,
bioRxiv - Bioengineering 2022
Quote:
... and Red Fluorescent Protein-tagged HUVEC (RFP-HUVEC, 2Bscientific, UK) were cultured on fibronectin-coated (PromoCell) plates up to passage 7 and maintained in Endothelial Growth Medium 2 (EGM2 ...
-
No products found
because this supplier's products are not listed.
Rayan Khaddaj-Mallat, et al.,
bioRxiv - Neuroscience 2021
Quote:
... The levels of S protein in different brain regions upon its intranasal infusion were measured using His-Tag protein ELISA kit that allows detection and quantification of poly-histidine-tagged proteins in a biological sample (Cell Biolabs Inc., CA, USA). The experimental procedure was performed as recommended by the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Sophie L. Granger, et al.,
bioRxiv - Biophysics 2024
Quote:
... All fluorophores used to generate fluorescently labeled proteins were purchased from Click Chemistry Tools. Cy3-labeled ...
-
No products found
because this supplier's products are not listed.
Yugo Morita, et al.,
bioRxiv - Immunology 2021
Quote:
... Mice intramuscularly infected with AAV expressing recombinant GFP (0.5 × 1012 genome copies/ mouse, Vector Biolabs) were used as controls.
-
No products found
because this supplier's products are not listed.
Yang Xu, et al.,
bioRxiv - Plant Biology 2019
Quote:
... and the fluorescence intensity of Venus-tagged protein was measured on a Synergy H4 Hybrid reader (Biotek, Winooskit, VT, USA) at excitation and emission wavelengths of 485 nm and 528 nm ...
-
No products found
because this supplier's products are not listed.
Pei Sun, et al.,
bioRxiv - Neuroscience 2020
Quote:
... a lAAV200,000-labeled MOp mouse sample and a cslAAV20,000-labeled basal forebrain mouse sample were embedded with Technovit 9100 Methyl Methacrylate (MMA, Electron Microscopy Sciences, USA) according to previous procedures with minor optimizations66 ...
-
No products found
because this supplier's products are not listed.
Xiaoling Qiang, et al.,
bioRxiv - Immunology 2020
Quote:
... Recombinant proteins were tested for LPS content by the chromogenic Limulus amebocyte lysate assay (Endochrome; Charles River), and the endotoxin content was less than 0.01 U per microgram of recombinant proteins.
-
No products found
because this supplier's products are not listed.
Emilio Boada-Romero, et al.,
bioRxiv - Cell Biology 2023
Quote:
Lipid binding capacity of recombinant proteins or anti-PS antibody were analyzed using lipid arrays (Echelon Biosciences Inc ...
-
No products found
because this supplier's products are not listed.
Serena Cortés-Kaplan, et al.,
bioRxiv - Immunology 2021
Quote:
... APC counting beads (Spherotech, IL) were added ...
-
No products found
because this supplier's products are not listed.
Pablo Lazcano, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Corresponding europium-tagged secondary antibodies (Molecular Devices) were used at a 1:5000 dilution and 1:20,000 for actin ...
-
No products found
because this supplier's products are not listed.
Shaun Scaramuzza, et al.,
bioRxiv - Cell Biology 2022
Quote:
... γ-H2AX (4418-APC-020, Trevigen). Affinity purified anti-Cdc45 ...
-
No products found
because this supplier's products are not listed.
Henriette Hoffmann-Veltung, et al.,
bioRxiv - Immunology 2022
Quote:
... 100 µL of either goat anti-mouse IgG (for 6E2 hybridoma cells) or mouse anti-human IgG (for EBV-immortalized human B cells) specific for the Fc region (Mabtech) diluted in sterile PBS at 15 µg/mL were added to wells followed by incubation at 4°C for 24 hours ...
-
No products found
because this supplier's products are not listed.
Robin Hoeven, et al.,
bioRxiv - Bioengineering 2019
Quote:
... Lysate samples were analysed for recombinant protein expression by SDS PAGE (12% Mini-PROTEAN-TGX stain-free gel; Bio-Rad), using Precision Plus unstained protein ladder (Bio-Rad ...
-
No products found
because this supplier's products are not listed.
Rosa Pipitone, et al.,
bioRxiv - Plant Biology 2020
Quote:
... and PsaC was performed according to Agrisera instructions and using recombinant proteins (PsbA AS01 0116S, PetC AS08 330S, and PsaC AS04 042S; Agrisera, Vännäs, SWEDEN). Three respective calibration curves for the three recombinant proteins were created ...
-
No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... 5 μg of His-tagged H2A:H2B or His-H3:Flag-H4 (Diagenode) and 2 μg of Flag-hSpt16 were added to 250 μl of binding buffer (50 mM Tris-HCl pH 7.4 ...
-
No products found
because this supplier's products are not listed.
Wooyoung Kang, et al.,
bioRxiv - Biophysics 2019
Quote:
... and Cy3-labeled ApU (250 μM, TriLink) for 30 min at 37 °C ...