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No products found
because this supplier's products are not listed.
Elena Arutyunova, et al.,
bioRxiv - Biophysics 2021
Quote:
... The fusion protein was subsequently digested with His-tagged SUMO protease (McLab, South San Francisco, CA) at 4 °C for 1–2 h to remove the SUMO tag and the resulting cleavage mixture was then passed through Ni-NTA resin column ...
-
No products found
because this supplier's products are not listed.
Camden R. Bair, et al.,
bioRxiv - Microbiology 2021
Quote:
... supplemented with 8% heat-inactivated newborn calf serum (HI-NBCS, Rocky Mountain Biologicals), 1.5 g/ml Na2CO3 (Amresco) ...
-
No products found
because this supplier's products are not listed.
Benjamin D. Hobson, et al.,
bioRxiv - Neuroscience 2022
Quote:
... A small hole was drilled into the skull and 400 nL of 0.9% saline or 0.9% saline + 100 ng/μL recombinant mouse IFN-γ (40 ng IFN-γ total) was injected through a pulled glass pipet using a Nanoject 2000 (Drummond Scientific; 8 pulses of 50 nL). At 5 minutes after injection ...
-
No products found
because this supplier's products are not listed.
Liangxi Wang, et al.,
bioRxiv - Genomics 2022
Quote:
... Cells were then treated with 10 ng/mL recombinant mouse TNFα (Cell Applications, cat# RP2031-20) for 45 min in basal Endothelial Cell Growth Media MV2 (PromoCell ...
-
No products found
because this supplier's products are not listed.
Gabrielle Brandt, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... recombinant human transferrin (InVitria), 80 μg/mL ...
-
No products found
because this supplier's products are not listed.
Siran Zhu, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... 150 pmol of 6×His-streptavidin (ProteoGenix) was mixed with 5 μl of sieved beads (10% ...
-
No products found
because this supplier's products are not listed.
Pramod Sahadevan, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Collagen-coated hydrogel-bound (8 kPa) polystyrene 35mm (PS35-COL-8) and 6-well (SW6-COL-8) plates were from Matrigen. Phos-tag acrylamide was from Wako Chemicals (#AAL-107) ...
-
No products found
because this supplier's products are not listed.
A Hendriks, et al.,
bioRxiv - Microbiology 2020
Quote:
... for IL-8 (Sanquin) and TNFα (Thermo Fisher ...
-
No products found
because this supplier's products are not listed.
Yeonoh Shin, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... 8 mM CHAPSO (Hampton research) was added to the reaction ...
-
No products found
because this supplier's products are not listed.
Huang Zhu, Dan S. Kaufman,
bioRxiv - Immunology 2019
Quote:
... 15 % heat-inactivated human AB serum (Valley Biomedical, Catalog # HP1022 HI),1 % P/S ...
-
No products found
because this supplier's products are not listed.
Ronald Rodriguez, et al.,
bioRxiv - Microbiology 2022
Quote:
... and AMK (Ambeed, 8 μg/ml). All cultures were prepared in triplicate ...
-
No products found
because this supplier's products are not listed.
Matheus F. Sathler, et al.,
bioRxiv - Neuroscience 2022
Quote:
... NIH: HIV-1 IIIB gp120 Recombinant Protein from ImmunoDX, LLC ...
-
No products found
because this supplier's products are not listed.
Advika Kamatar, et al.,
bioRxiv - Biophysics 2023
Quote:
... His-Clathrin was labeled with Atto594 NHS-ester (ATTO-TEC, Sigma-Aldrich) according to a previously published protocol (41 ...
-
No products found
because this supplier's products are not listed.
Ben S. Ou, et al.,
bioRxiv - Immunology 2023
Quote:
... Heat Inactivated fetal bovine serum (HI-FBS) was purchased from Atlanta Biologicals. IFN-α cytokine enzyme-linked immunosorbent assay (ELISA ...
-
No products found
because this supplier's products are not listed.
Duško Lainšček, et al.,
bioRxiv - Immunology 2020
Quote:
... 6-8 kDa cutoff (Spectrum Laboratories, USA). Samples were purified using NiNTA resin (Goldbio ...
-
No products found
because this supplier's products are not listed.
Lochlain Corliss, et al.,
bioRxiv - Microbiology 2022
Quote:
... in an 8-well chamber slide (Celltreat). Twenty-four hours post transfection ...
-
No products found
because this supplier's products are not listed.
Marisa Oliveira, et al.,
bioRxiv - Immunology 2020
Quote:
... and chicken interferon alpha (Yeast-derived Recombinant Protein, Kingfisher Biotech, Inc) were diluted in nuclease-free water (Ambion ...
-
No products found
because this supplier's products are not listed.
Katharina MC Klee, et al.,
bioRxiv - Cell Biology 2022
Quote:
Anoctamin 8 (WB 1:500, #HPA049206, Atlas Antibodies), ARHGAP33 (Western-blotting (WB ...
-
No products found
because this supplier's products are not listed.
Jorge Gomez-Deza, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and 8 mg/ml polybrene (Thomas Scientific; #C788D57) and mixed with a volume of KIDHGW lentivirus corresponding to an 1600x representation with MOI of 0.3 ...
-
No products found
because this supplier's products are not listed.
Melisa Lázaro, et al.,
bioRxiv - Microbiology 2020
Quote:
... This was confirmed by labeling N-terminally His6-tagged mL-GDH180 with Ni-NTA-Nanogold (Nanoprobes) and visualizing particles by negative staining electron microscopy ...
-
No products found
because this supplier's products are not listed.
Jeanette M. Metzger, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Nuclear localization signal (NLS)-tagged Streptococcus pyogenes Cas9 nuclease (sNLS-SpCas9-sNLS) was obtained from Aldevron. In vitro transcribed single guide RNAs (sgRNAs ...
-
No products found
because this supplier's products are not listed.
Wiktoria Ogrodzińska, et al.,
bioRxiv - Biochemistry 2024
Quote:
... The recombinant plasmids were isolated using plasmid purification kit (Bio Basic Canada) and the inserts were verified by sequencing (Genomed ...
-
No products found
because this supplier's products are not listed.
Hu Zhu, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Compound 8 was purchase from Enamine (Monmouth Junction, NJ). Amplex Red (10-Acetyl-3,7-dihydroxyphenoxazine ...
-
No products found
because this supplier's products are not listed.
Tomofumi Nakamura, et al.,
bioRxiv - Microbiology 2019
Quote:
Recombinant INHis proteins were examined with a TSKgel-SWXLG3000 column (TOSOH cor., Japan) at 1.0 ml/min in running buffer (20 mM HEPES pH 7.4 ...
-
No products found
because this supplier's products are not listed.
Nicolas Lamassiaude, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... Recombinant constructs were purified using EZNA Plasmid DNA Mini kit (Omega Bio-Tek) and sequence-checked (Eurofins Genomics) ...
-
No products found
because this supplier's products are not listed.
Mario Ivankovic, et al.,
bioRxiv - Genomics 2023
Quote:
... All libraries ran on SMRT™ Cells 8 M (PacBio) using the Sequel® II Sequencing Kit 2.0 (PacBio ...
-
No products found
because this supplier's products are not listed.
Yao Liu, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and phosphatase inhibitors (AG Scientific) as previously described [38] ...
-
No products found
because this supplier's products are not listed.
Ana Eufrásio, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 100 U Ribonuclease Inhibitor (NZYtech) and 60U of SP6 RNA polymerase (Thermo Fisher ...
-
No products found
because this supplier's products are not listed.
Federico Bertoglio, et al.,
bioRxiv - Biochemistry 2020
Quote:
... supplemented with 8 mM Glutamine and 0.1% Pluronic F68 (PAN Biotech). At the day of transfection cell density was between 1.5 - 2×106 cells/mL and viability at least above 90% ...
-
No products found
because this supplier's products are not listed.
Sara Abdulkader, John Gigg,
bioRxiv - Animal Behavior and Cognition 2024
Quote:
Training took place in 8 operant chambers (Campden instruments Ltd, UK), each placed inside a ventilated and sound-attenuating box ...
-
No products found
because this supplier's products are not listed.
Sadra Bakhshandeh, et al.,
bioRxiv - Bioengineering 2023
Quote:
... the following small molecule and inhibitors were used: MEK/ERK inhibitor PD98059 (10 mM, Focus Biomolecules), JNKinhibitor SP600125 (10 mM ...
-
No products found
because this supplier's products are not listed.
Nandan Haloi, et al.,
bioRxiv - Biophysics 2020
Quote:
... followed by addition of HKII (human recombinant HKII, 60 kU/ml; Genway Biotech, Slan Diego, CA) into the cis chamber ...
-
No products found
because this supplier's products are not listed.
Axel Guilbaud, et al.,
bioRxiv - Biochemistry 2022
Quote:
... and [15N5]-8-oxo-2’-deoxyguanosine were purchased from Toronto Research Chemical (Toronto, Canada). 5’-Ethynyl-2’-deoxycytidine and 5’-chloro-2’- deoxycytidine were obtained from Cayman Chemical Company (Ann Arbor ...
-
No products found
because this supplier's products are not listed.
Jiechao Zhou, et al.,
bioRxiv - Neuroscience 2022
Quote:
... mouse sera (Complement Technology) was incubated with C1q (4.8 ...
-
No products found
because this supplier's products are not listed.
Zhaowei Wang, Xiaoling Xia, Tatsushi Igaki,
bioRxiv - Cell Biology 2022
Quote:
... The proteasome inhibitor lactacystin (Peptide Institute, Code #4369-v) was used at 10μM ...
-
No products found
because this supplier's products are not listed.
Kyle S Severson, et al.,
bioRxiv - Neuroscience 2019
Quote:
... the incision was closed with fine suture thread (8/0, Fine Science Tools #12051-08) and coated with antibiotic ointment (Pac-Kit) ...
-
No products found
because this supplier's products are not listed.
Robin Hoeven, et al.,
bioRxiv - Bioengineering 2019
Quote:
... Lysate samples were analysed for recombinant protein expression by SDS PAGE (12% Mini-PROTEAN-TGX stain-free gel; Bio-Rad), using Precision Plus unstained protein ladder (Bio-Rad ...
-
No products found
because this supplier's products are not listed.
Gregory J. Ordemann, et al.,
bioRxiv - Neuroscience 2021
Quote:
Extracellular stimulation was performed using bipolar sharp tungsten electrodes (5 MΩ, 8° taper, A-M Systems) connected to a Neurolog NL800A current stimulus isolator (Digitimer) ...
-
No products found
because this supplier's products are not listed.
Kévin Leguay, et al.,
bioRxiv - Cell Biology 2020
Quote:
... inhibitors were added using an Echo 555 acoustic dispenser (Labcyte) while BRET2 was monitored with a Synergy NEO HTS microplate reader (Biotek).
-
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Richard J. Roller, et al.,
bioRxiv - Microbiology 2021
Quote:
... or mouse anti-VP5 (Biodesign. International) 1:500 ...
-
No products found
because this supplier's products are not listed.
Xiaodan Zhang, et al.,
bioRxiv - Genomics 2021
Quote:
... anti-mouse Lyve1 antibody (1:100, AngioBio cat.no ...
-
No products found
because this supplier's products are not listed.
Rita R. Fagan, et al.,
bioRxiv - Neuroscience 2020
Quote:
... mouse anti-SERT (ST51-2; Mab Technologies), rabbit anti-HA (C29F4 ...
-
No products found
because this supplier's products are not listed.
Remi Bos, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Mouse monoclonal to Islet-1 (Biorbyt, #Orb95122)(1:200) ...
-
No products found
because this supplier's products are not listed.
Jiechao Zhou, et al.,
bioRxiv - Neuroscience 2022
Quote:
... C1q mouse ELISA (Hycult Biotech, HK211-01), Total C4 (LSBio ...
-
No products found
because this supplier's products are not listed.
Robert Wimbish, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Silanized coverslips were incubated with a rat anti-tubulin antibody (8 μg/ml, YL1/2; Accurate Chemical & Scientific Corporation) for 5 minutes ...
-
No products found
because this supplier's products are not listed.
Jasmin N. Beaver, et al.,
bioRxiv - Neuroscience 2023
Quote:
... all mouse cages contained Nestlets (Ancare, Bellmore, NY) and huts ...
-
No products found
because this supplier's products are not listed.
A Elgheznawy, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Platelets were activated with appropriately diluted agonists for 8 min at 37°C followed by 8 min at RT in the presence of saturating amounts of PE-coupled JON/A (4H5, Emfret Analytics) detecting activated αIIbβ3 integrin and FITC-coupled anti-P-selectin (Wug.E9 ...
-
No products found
because this supplier's products are not listed.
Gokul Swaminathan, et al.,
bioRxiv - Immunology 2021
Quote:
... following the Mouse Anti-OVA IgG Antibody Assay Kit (Chondrex), or as previously described (76).
-
No products found
because this supplier's products are not listed.
Lu Zhang, et al.,
bioRxiv - Neuroscience 2022
Quote:
Adult mice of 6-8 weeks were anaesthetized (i.p. 100mg/kg sodium pentobarbital) and mounted on a stereotaxic frame (RWD Life Science, Shenzhen, China). Virus suspension (AAV9-CaMKII-mCherry ...
-
No products found
because this supplier's products are not listed.
Seraina A. Domenig, et al.,
bioRxiv - Cell Biology 2023
Quote:
... at 37°C using DirectPCR Lysis Reagent (mouse tail) (VIG102-T, Viagen Biotech). PCR for Pax7-nGFP was performed using primer Pax7nGFP.F/ Pax7nGFP.R and GoTaq G2 Hot Start Green Master Mix (M7423 ...