-
No products found
because this supplier's products are not listed.
Fangyuan Ding, et al.,
bioRxiv - Systems Biology 2020
Quote:
... were coated with 5 ug/ml Human Fibronectin (Oxford Biomedical Research, Rochester Hills, MI) in PBS buffer for 1hr at room temperature ...
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No products found
because this supplier's products are not listed.
Rebecca L. Murray, et al.,
bioRxiv - Microbiology 2021
Quote:
CK2 was inactivated using the CK2-specific inhibitor CX-4945 (0308, Advanced Chemblocks). HeLa cells were seeded and transfected with CFP-VAP DNA the following day (for immunofluorescence assay only) ...
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No products found
because this supplier's products are not listed.
Max G. Schubert, et al.,
bioRxiv - Microbiology 2023
Quote:
... then sparged into the cultures using a 5 X 210mm porosity B glass filter stick (Ace Glass).
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No products found
because this supplier's products are not listed.
Ken Shirato, Jun Takanari, Takako Kizaki,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... the cells were co-treated with the indicated concentrations of ETAS®50 or dextrin and 100 ng/mL of SARS-CoV-2 spike recombinant protein S1 subunit (Arigo Biolaboratories, Hsinchu City, Taiwan) for 1 to 24 h ...
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No products found
because this supplier's products are not listed.
Qing Zhao, et al.,
bioRxiv - Immunology 2023
Quote:
... and then incubated with Dylight 550 Goat anti-human IgA (ImmunoReagents, Cat # GtxHu-001-E550NHSX) and Dylight 650 Donkey anti-human IgG (Invitrogen ...
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No products found
because this supplier's products are not listed.
Carole J Kuehl, et al.,
bioRxiv - Microbiology 2019
Quote:
... diluted in PBS with 0.5% BSA and 0.5% Triton X-100: anti-Shigella-FITC (1/1000; #0903, Virostat); anti-E-cadherin 1:100 (610181 ...
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No products found
because this supplier's products are not listed.
Stephanie Dobersch, et al.,
bioRxiv - Molecular Biology 2020
Quote:
Hydroxyproline levels in human lung fibroblasts were determined using the QuickZyme Hydroxyproline Assay kit (Quickzyme Biosciences). The cells and lung tissue were separately homogenized in 1 ml 6 N HCl with a Precellys tissue homogenizer (2 × 20 s ...
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No products found
because this supplier's products are not listed.
Kathleen M.E. Gallagher, et al.,
bioRxiv - Immunology 2021
Quote:
A qualitative ELISA for Human Anti-IgG/A/M SARS-CoV-2 ELISA (The Binding Site) was performed using donor serum per manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Naomi G. Wilson, et al.,
bioRxiv - Microbiology 2022
Quote:
Human fecal calprotectin was measured using the Calprotectin ELISA Assay Kit (Eagle Biosciences cat. CAL35-K01) following manufacturer’s directions ...
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No products found
because this supplier's products are not listed.
Ane Olazagoitia-Garmendia, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... BTX electroporator ECM) were then applied to the brain between insulated silver 40mm x 0.8mm wire electrodes with flattened pole (Intracel). Drops of Ringer’s solution supplemented with antibiotics (penicillin/streptamycin ...
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No products found
because this supplier's products are not listed.
Andrew Barszczyk, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 4.5 x 104 dissociated cells in 60 μl of plating medium were plated on 12 mm coverslips (#194310012; Bellco) pre-coated with 0.1 mg/ml poly-D-lysine hydrobromide solution (#P0899 ...
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No products found
because this supplier's products are not listed.
Kirsten L. Viola, et al.,
bioRxiv - Neuroscience 2021
Quote:
... poly-L-lysine coated coverslips plated at a density of 1.04 x 104 cells per cm2 in Neurobasal media (Brainbits, LLC) with B27 supplements and L-glutamine (2.5 μM).
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No products found
because this supplier's products are not listed.
Paule Dagenais, et al.,
bioRxiv - Biophysics 2021
Quote:
... excisions of rays were conducted using a micro dissecting knife (RS-6220, dean knife 5” 1 mm x 7 mm blade curved, Roboz Surgical Instrument Co. ...
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No products found
because this supplier's products are not listed.
Kevin K. Chi, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 0.1% Triton-X in PBS) followed by incubation (1 hr) of α-dsRNA J2 primary antibody (1:100; SCICONS, U.S.). Cover slips were washed with PBST and incubated with secondary α-IgG AlexaFluor antibody (1:1000 ...
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No products found
because this supplier's products are not listed.
Elana M. Meijer, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 3 x 3 dotted patterns were created on the membranes of a 6-well Bioflex culture plate (untreated, Flexcell Int). Videos were captured at day 3 (the first day of straining ...
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No products found
because this supplier's products are not listed.
Emelyne Teo, et al.,
bioRxiv - Neuroscience 2019
Quote:
... 1X Protease inhibitors and 1mM PMSF) and homogenized using a Bead Beater (Benchmark Scientific, Edison, USA). To obtain the detergent-soluble protein ...
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No products found
because this supplier's products are not listed.
Tianyang Mao, et al.,
bioRxiv - Immunology 2021
Quote:
The triphosphorylated RNA oligonucleotides SLR-14 (5′ pppGGAUCGAUCGAUCGUUCGCGAUCGAUCGAUCC-3′ and SLR-14-amino (5′ pppGGAUCGAUCGAUCGUXCGCGAUCGAUCGAUCC-3′ where X = aminomodifier C6dT; Glen Research) were prepared as described57 ...
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No products found
because this supplier's products are not listed.
Klemens Winkler, et al.,
bioRxiv - Biophysics 2020
Quote:
... USA) and was focused on the sample via a 40 x 1.25 glycerol immersion quartz objective (Sysmex Partec GmbH, Münster, Germany). The objective also served to collect the light emitted by Tb3+ or the acceptor molecules ...
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No products found
because this supplier's products are not listed.
Jennifer Molinet, et al.,
bioRxiv - Microbiology 2022
Quote:
... cells were collected by centrifugation at 5,000 x g for 5 min and treated with two units of Zymolyase (Seikagaku Corporation, Japan) for 30 min at 37 °C ...
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No products found
because this supplier's products are not listed.
Laura A. Hehl, et al.,
bioRxiv - Biochemistry 2023
Quote:
... using 4 x fold excess of amino acids relative to pre-loaded Fmoc amino acid trityl resin (0.2 mmol/g, Rapp Polymere GmbH).
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No products found
because this supplier's products are not listed.
A Castellanos-Gonzalez, et al.,
bioRxiv - Microbiology 2022
Quote:
Human ileocecal cells (HCT-8 cells, ATCC, Manassas, VA) and Cryptosporidium parvum parasites (Waterborne, INC, New Orleans, LA) were used for in vitro studies ...
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No products found
because this supplier's products are not listed.
Katlijn De Meulenaere, et al.,
bioRxiv - Microbiology 2022
Quote:
... after which the parasites were harvested and remaining human white blood cells were depleted with Plasmodipur filtration (EuroProxima) as described by Auburn et al ...
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No products found
because this supplier's products are not listed.
Yvonne Giesecke, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... Quantitative real-time human-specific primer sets for CyberGreen PCR amplifications were obtained from TIB Molbiol (Berlin, Germany). Primer sequences were:
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No products found
because this supplier's products are not listed.
Alina Sigaeva, et al.,
bioRxiv - Cell Biology 2023
Quote:
Human colon adenocarcinoma HT-29 cells were routinely cultured in Dulbecco’s Modified Eagle’s Medium (Cellutron Life Technologies, USA) with a high glucose concentration ...
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No products found
because this supplier's products are not listed.
S. L. Fowler, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Integrated FRET densities (% FRET positive cells per gated cell population x median fluorescence intensity of the BV510 signal) were obtained using FCS Express 7 Research software (De Novo Software).
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No products found
because this supplier's products are not listed.
Samar Ahmad, et al.,
bioRxiv - Neuroscience 2023
Quote:
Exosome pellets (100,000 x g) were also purified using qEV original size exclusion column with a pore size of 70 nm (Izon science, 1001683) according to manufacturer’s protocol with minor modifications ...
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No products found
because this supplier's products are not listed.
Hanna Englert, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... the membranes were incubated with a goat anti-human FXII antibody (1:500, GAHu/FXII, Nordic-MUbio, The Netherlands) for 1h at RT ...
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No products found
because this supplier's products are not listed.
Thi Tuyet Trinh Phan, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... and 0.5% sodium deoxycholate) supplemented with complete protease and phosphatase inhibitor cocktails (Fivephoton Biochemicals, San Diego, CA, USA). Cell suspension was subsequently incubated on ice for 10 min and then vortexed vigorously for 5 sec ...
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No products found
because this supplier's products are not listed.
Jonathan M Budzik, et al.,
bioRxiv - Microbiology 2019
Quote:
Immunostaining for LC3 was performed with blocking and permeabilization buffer (0.3% Triton X-100, 2% bovine serum albumin) and anti-LC3 (clone 2G6, Nanotools #0260-100/LC3-2G6) at a dilution of 1:200 for 3 hours at room temperature ...
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No products found
because this supplier's products are not listed.
R. Patrick Xian, et al.,
bioRxiv - Physiology 2021
Quote:
We used a PET (polyethylene terephthalate) jar of comparable size to the lung for X-ray imaging due to its commercial availability (Medline Scientific, 3600 mL), high radiation tolerance31 and optical transparency in assisting sample alignment and assessment of sample condition during imaging ...
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No products found
because this supplier's products are not listed.
Steven Johnson, et al.,
bioRxiv - Microbiology 2020
Quote:
... 0.03% Triton X-100 in 5 mL 300 kDa molecular weight cut-off (MWCO) Float-A-Lyzer®G2 Dialysis Device (Spectrum Laboratories, Inc.) overnight ...
-
No products found
because this supplier's products are not listed.
Lisa C. Hiura, et al.,
bioRxiv - Neuroscience 2023
Quote:
... PBS + 10% normal donkey serum + 0.03% Triton-X-100) before being incubated in primary antibodies (48 h, Guinea Pig anti-VP 1:1000, Peninsula Laboratories, San Carlos, CA). Sections were rinsed in PBS (2 x 30m) ...
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No products found
because this supplier's products are not listed.
Naushin L. Hindul, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The starved cells were growth-factor stimulated with fresh DMEM media containing 100ng/ml epidermal growth factor (EGF Human #A63411-500 (Antibodies.com)) ...
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No products found
because this supplier's products are not listed.
Julia Hansen, et al.,
bioRxiv - Microbiology 2022
Quote:
... ethyl-[R]-cysteinyl-[S]-lysyl-[S]-lysyl-[S]-lysyl-[S]-lysyl-[S]-lysine(ε-aminocaproyl-∈-aminocaproyl-biotinyl) x 3 CF3COOH (EMC Microcollections, Tübingen, Germany) at 1.5 mg/ml in carbonate buffer (pH 9.2 ...
-
No products found
because this supplier's products are not listed.
Yasuaki Uehara, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Phosphate homeostasis hormones were measured in mouse and human serum using the FGF-23 ELISA Kit (Kainos Laboratories Inc., Tokyo, Japan), the mouse or human PTH 1-84 ELISA Kit (Immutopics ...
-
No products found
because this supplier's products are not listed.
Tomohiko Sadaoka, et al.,
bioRxiv - Microbiology 2020
Quote:
DNA and RNA from VZV-infected human sensory neurons were isolated using the FavorPrep Blood/Cultured Cell Total RNA Mini Kit (Favorgen Biotech) in combination with the NucleoSpin RNA/DNA buffer set (Macherey-Nagel) ...
-
No products found
because this supplier's products are not listed.
Jessica B. Blackburn, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 25 μg of SIgA was incubated with 10 μg sputum-derived human neutrophil elastase (1 ug/uL in 0.05 M NaOAc pH 5 containing 0.1 M NaCl, Elastin Products Company, SE563GI) with or without a protease inhibitor (Sigma ...
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No products found
because this supplier's products are not listed.
Ryouhei Tsutsumi, et al.,
bioRxiv - Cell Biology 2023
Quote:
... EGFP-conjugated GLUT1 ligand (receptor binding domain of the human T cell leukemiavirus (HTLV) envelope glycoprotein) was purchased from Metafora Biosystems.
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No products found
because this supplier's products are not listed.
Yunwei Lu, et al.,
bioRxiv - Systems Biology 2024
Quote:
We performed eY1H assays using a human TF yeast array (15) as previously described and as follows using a high-density array ROTOR robot (Singer Instruments). The three-plate human TF yeast array and promoter yeast strains were mated pairwise on permissive media agar plates and incubated at 30°C for 1 day ...
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No products found
because this supplier's products are not listed.
Elizabeth V. K. Ledger, Andrew M. Edwards,
bioRxiv - Microbiology 2023
Quote:
... or C3 was detected with a 1:1000 dilution of goat anti-human C3 F(ab’)2 labelled with FITC (Protos Immunoresearch). Antibody incubations were carried out statically for 1 h at room temperature in the dark ...
-
No products found
because this supplier's products are not listed.
Xiao Han, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 5 grams of yeast powder was dissolved in 30 mL binding buffer with 50 µL protease inhibitor cocktail IV (BioWorld), and 300 µL of 100 mM PMSF ...
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No products found
because this supplier's products are not listed.
Elena Sánchez Martín-Fontecha, et al.,
bioRxiv - Plant Biology 2024
Quote:
Seven-dpg seedlings grown horizontally in MS medium were pre-treated with the proteasome inhibitors 50 μM MG132 and 20 nM epoxomicin for 1 h and then with 10 μM GR24rac (Strigolab) for further 3 h at 22°C ...
-
No products found
because this supplier's products are not listed.
Xue Yang, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... The SFK inhibitor eCF506 was obtained from Professor Margaret Frame (Edinburgh Cancer Research Centre). Captisol (cat. A508148) was purchased from AmBeed. BAPTA-AM (cat ...
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No products found
because this supplier's products are not listed.
Rania Francis, et al.,
bioRxiv - Microbiology 2019
Quote:
Two cell lines were used as cellular supports for co-culture: the human embryonic lung fibroblast MRC5 cells (RD-Biotech, Besançon, France) and the mouse fibroblast L929 cells (ATCC® CCL-1) ...
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No products found
because this supplier's products are not listed.
Marlou L. Dirks, et al.,
bioRxiv - Physiology 2023
Quote:
... Arterialized serum samples were used to determine insulin concentrations (Human insulin ELISA kit, DX-EIA-2935; Oxford Biosystems Ltd, Milton Park, UK). Serum NEFA concentrations were measured spectrophotometrically in arterialized venous and deep-venous serum samples (FA115 kit ...
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No products found
because this supplier's products are not listed.
C. Keating, et al.,
bioRxiv - Microbiology 2020
Quote:
... The mixture containing 0.25 g of digesta content was lysed by bead beating for 10 min at 3.2 K x g in a Vortex-Genie2™ (Scientific Industries Inc. Bohemia, New York, USA) and phase separation achieved by centrifugation at 13.3K x g for 10 minutes ...
-
No products found
because this supplier's products are not listed.
Noriyo Nagata, et al.,
bioRxiv - Pathology 2021
Quote:
... Immunohistochemical analysis was performed using a polymer-based detection system (Nichirei-Histofine Simple Stain Human MAX PO®; Nichirei Biosciences, Inc., Tokyo, Japan). Antigen retrieval from formalin-fixed monkey tissue sections was performed by autoclaving in retrieval solution (pH 6.0 ...
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No products found
because this supplier's products are not listed.
Jerome Perrard, Susan Smith,
bioRxiv - Biochemistry 2023
Quote:
... Tankyrase inhibitor #8 (TNKSi)(Haikarainen et al., 2013) was used at 10 µM for 24 h (Chembridge Corporation, MolPort-000-222-699). For U2OS shUB transfections ...
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Cat# AB-286,
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Judith Jing Wen Wong, Pål Kristian Selbo,
bioRxiv - Cancer Biology 2021
Quote:
The immunotoxin anti-human PD-L1-saporin (Anti-PD-L1-SAP, BETA-014) was generously provided by Advanced Targeting Systems (ATS, Carlsbad, CA, USA). The targeted toxin is a 295 kDa conjugate between a rabbit polyclonal antibody to human PD-L1 and the secondary conjugate streptavidin-saporin ...
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No products found
because this supplier's products are not listed.
Miguel A. Olivencia, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
25-hydroxyvitamin D and testosterone levels were measured in rat citrated plasma and human EDTA plasma using a General 25-Hydroxyvitamin D3 (HVD3) ELISA Kit (Reddot Biotech Inc., Kelowna, Canada) and Testosterone ELISA DE1559 (Demeditec ...