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No products found
because this supplier's products are not listed.
Elena Arutyunova, et al.,
bioRxiv - Biophysics 2021
Quote:
... The fusion protein was subsequently digested with His-tagged SUMO protease (McLab, South San Francisco, CA) at 4 °C for 1–2 h to remove the SUMO tag and the resulting cleavage mixture was then passed through Ni-NTA resin column ...
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No products found
because this supplier's products are not listed.
Menglin Shi, Lei Zhao, Yong Wang,
bioRxiv - Plant Biology 2021
Quote:
The recombinant HPRs was purified with HIS-Select nickel affinity gel filler (CoWin Biosciences, Beijing, China). Briefly ...
-
No products found
because this supplier's products are not listed.
Thomas Rowe, et al.,
bioRxiv - Microbiology 2024
Quote:
... 180 uL of HEK-λ cells were added to each well containing twenty uL of sample and to serially 1/2-log diluted (0.1 – 1000 ng/mL) recombinant ferret IFNL3 (Kingfisher Biotech, St. Paul, MN, USA). The plates were incubated for 20Hr at 37°C ...
-
No products found
because this supplier's products are not listed.
Stanimir S. Ivanov, et al.,
bioRxiv - Microbiology 2021
Quote:
The human monocytic cell line U937 (ATCC CRL-1593.2) was obtained from ATCC and primary human peripheral monocytic cells (PBMCs) from two different donors were purchased from Precision for Medicine. U937 cells were cultured in RPMI 1640 media (VWR ...
-
No products found
because this supplier's products are not listed.
Chanchal Thomas Mannully, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... 1 μg/mL of human TGFβ1 (Biogems, Peprotech).
-
No products found
because this supplier's products are not listed.
Aman Y. Husbands, et al.,
bioRxiv - Plant Biology 2022
Quote:
... Membranes were washed three times with PBS-T and incubated with 1:2000 anti-His-tag antibody (Abiocode M0335-1) for 1h with gentle shaking at RT ...
-
No products found
because this supplier's products are not listed.
Hyunjoon Kim, Soohyun Jang, Young-suk Lee,
bioRxiv - Molecular Biology 2021
Quote:
... cells were selected with medium containing 1∼2 μg/ml puromycin (AG Scientific #P-1033) until non-transduced cells became completely dead ...
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Cat# G209,
USD $10.00/EA
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Glory Nasseri, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Human PRG-1 obtained from DNASU Plasmid Repository (The Biodesign Institute/Arizona State University ...
-
No products found
because this supplier's products are not listed.
Xuan He, et al.,
bioRxiv - Immunology 2021
Quote:
... The plates were washed with coulter buffer and incubated for 2 h with Rabbit polyclonal anti-human IFN-γ Biotin from U-Cytech (1 μg/mL). The plates are washed a second time and incubated for 2 h with Streptavidin-alkaline phosphatase antibody from Southern Biotechnology (1 μg/mL) ...
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ST2 Human ELISA / assay Kit
Cat# K055-H1,
1.0 ea, USD $505.0
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Nina Kessler, et al.,
bioRxiv - Immunology 2022
Quote:
cGAMP in human PBMCs was quantified with the 2’,3’-Cyclic GAMP ELISA Kit (Arbor assays) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Rafi Rashid, et al.,
bioRxiv - Microbiology 2022
Quote:
... An equal volume of human β-defensin 2 (hBD2) or LL-37 (Peptide Institute Inc., Japan) was added to the samples and incubated statically for 2 hours at 37oC (Table S1G) ...
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WB, ELISA
Cat# CDC-41,
0.1 mg, Inquire
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Yunjeong Kim, et al.,
bioRxiv - Microbiology 2021
Quote:
... Vero E6 cells expressing human TMPRSS2 (Vero-TMPRSS2) cells were obtained from Creative Biogene (Shirley, NY)[88] ...
-
No products found
because this supplier's products are not listed.
Chetan Kumar Arya, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and Wizard Classic 1 and 2 (Rigaku Japan). Crystals of DMFase were obtained by mixing 200 nl of protein in buffer A with equal volumes of precipitant ...
-
No products found
because this supplier's products are not listed.
Jared R. Bagley, et al.,
bioRxiv - Animal Behavior and Cognition 2021
Quote:
... The back wall of the box is attached to the mouse cage (7 1/2” × 11 1/2” × 5”, N10 Polycarbonate Mouse Cage, Ancare, NY USA) via thumbscrews or hinges attached with thumb screws for the servo access-control version ...
-
No products found
because this supplier's products are not listed.
Kristoffer Krogerus, Nils Rettberg, Brian Gibson,
bioRxiv - Microbiology 2022
Quote:
... after which spores from the different parental strains were dissected and placed next to each other on YPD agar plates (1 % yeast extract, 2 % peptone, 2 % glucose, and 2 % agar) using a micromanipulator (Singer Instruments, UK). The plates were incubated at 25 °C for 3 days ...
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No products found
because this supplier's products are not listed.
Christopher R. Brown, James D. Foster,
bioRxiv - Neuroscience 2024
Quote:
... anti-mouse NET-05 and anti-human NET17-1 antibodies were from Mab Technologies. N-ethylmaleimide (NEM) ...
-
No products found
because this supplier's products are not listed.
Atanas Radkov, et al.,
bioRxiv - Biochemistry 2022
Quote:
... PG ligand was dialyzed against water using a float dialysis membrane device with a 100-500 Da cut-off (Spectrum Laboratories, Inc) and lyophilized before preparation of a concentrated stock solution in NMR buffer ...
-
No products found
because this supplier's products are not listed.
Sylvie Roy, et al.,
bioRxiv - Microbiology 2020
Quote:
... Serial dilutions of known amounts of C-terminally Fc-tagged S2 (BioVendor, Brno, Czech Republic) were used for quantification.
-
No products found
because this supplier's products are not listed.
Lisa Maria Metz, et al.,
bioRxiv - Cell Biology 2022
Quote:
... GPIbα (CD42b, Xia.G5-PE, #M040-2 Emfret Analytics, 1:10), GPVI (JAQ1-FITC ...
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No products found
because this supplier's products are not listed.
Jonah C. Rosch, et al.,
bioRxiv - Bioengineering 2021
Quote:
... Fluorescently labeled human serum albumin (HS1-S5-1) was purchased from NANOCS (New York, NY). The starting DNA library ...
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No products found
because this supplier's products are not listed.
Jeanette M. Metzger, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Nuclear localization signal (NLS)-tagged Streptococcus pyogenes Cas9 nuclease (sNLS-SpCas9-sNLS) was obtained from Aldevron. In vitro transcribed single guide RNAs (sgRNAs ...
-
No products found
because this supplier's products are not listed.
Shuo Yang, et al.,
bioRxiv - Microbiology 2022
Quote:
... anti-SARS-CoV-2-ORF3a (1:250; 101AP, FabGennix International Inc), anti-Actin (1:500 ...
-
No products found
because this supplier's products are not listed.
Min Pan, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Cas9 activity was tested using the CRISPRtest™ Functional Cas9 Activity Kit for Human Cells (Cellecta, #CRTEST). The clone with normal viability and high Cas9 activity (> 95% ...
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No products found
because this supplier's products are not listed.
Hannah L. Bernstein, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Slices were in the chamber for 30 min with sucrose-based ACSF at 1-2 mL/min (#Minpuls 2, Gilson) and then NaCl-based ACSF (125 mM NaCl instead of sucrose ...
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No products found
because this supplier's products are not listed.
Ng Anthony Yong Kheng Cordero, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The following antibodies or staining reagents were used: Mouse-anti human CD11b-FITC (Bear-1, Hycult Biotech), mouse-anti human CD62L-Alexa Fluor 647 (DREG-56 ...
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No products found
because this supplier's products are not listed.
Jonathon A.B. Smith, et al.,
bioRxiv - Physiology 2024
Quote:
... and glucose uptake was measured in the same medium by adding 1 uL.mL-1 of 2-[1,2-3H]Deoxy-D-glucose (MT911, Moravek) and 10 μM unlabelled 2-Deoxy-D-glucose in for 15 min [32] ...
-
No products found
because this supplier's products are not listed.
Charles A. Specht, et al.,
bioRxiv - Immunology 2021
Quote:
... the recombinant proteins were resolved on SDS-PAGE and stained with Coomassie InstantBlue (Expedeon, Ltd.).
-
No products found
because this supplier's products are not listed.
Erin J. Vanzyl, et al.,
bioRxiv - Cell Biology 2020
Quote:
... ATF3 mRNA expression was determined using Bioline SensiFAST™ Probe HI-ROX Master Mix (FroggaBio Inc, Toronto, ON) with SYBR green ...
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No products found
because this supplier's products are not listed.
Julianne Meisner, et al.,
bioRxiv - Microbiology 2022
Quote:
... 2) 1:100 dilution of test sera (diluted in ChronBlock ELISA Buffer-Chondrex Inc.); 3 ...
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No products found
because this supplier's products are not listed.
Caroline Murawski, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and 2 µm S1818 (Microchem, 5000 rpm, baking at 100°C for 1 min). Patterns were developed for 40 – 50 s in MF319 (Microchem) ...
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No products found
because this supplier's products are not listed.
Khomgrit Morarach, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Cells were resuspended in DPBS containing 1% BSA and 1% DRAQ7 (Biostatus). Tomato+ cells were sorted on a BD Influx™ cell sorter ...
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No products found
because this supplier's products are not listed.
Tomohiko Sadaoka, et al.,
bioRxiv - Microbiology 2020
Quote:
DNA and RNA from VZV-infected human sensory neurons were isolated using the FavorPrep Blood/Cultured Cell Total RNA Mini Kit (Favorgen Biotech) in combination with the NucleoSpin RNA/DNA buffer set (Macherey-Nagel) ...
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No products found
because this supplier's products are not listed.
Eric Vallabh Minikel, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Samples were injected (1 to 2 µg) onto a 75 um ID PicoFrit column (New Objective) packed to 20 cm with Reprosil-Pur C18 AQ 1.9 um media (Dr ...
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No products found
because this supplier's products are not listed.
Hanyuan Shen, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
A431 cells were treated with different injections for 48 hours in 96-well plates and the cell culture supernatant was collected and tested for the level of IL-1β by ELISA using human interleukin-1 beta ELISA kit (Biosensis, CA, USA) according to the kit protocol ...
-
No products found
because this supplier's products are not listed.
Zhimin Liu, et al.,
bioRxiv - Systems Biology 2020
Quote:
... ∼7.4 × 109 of frozen cells were inoculated into 1.2 L media in a 2 L Delong flask (Bellco). The cells were grown for a total of 21 generations ...
-
No products found
because this supplier's products are not listed.
Kojiro Suda, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Cells were incubated in 98 μl Sorbitol buffer + 2 μl of 2.5 mg/ml Zymolyase 100T (SEIKAGAKU CORPORATION) for 90 min (wild type ...
-
No products found
because this supplier's products are not listed.
Dorit Möhrle, et al.,
bioRxiv - Animal Behavior and Cognition 2023
Quote:
... 2) KD (weight ratio of fat to carbohydrate plus protein is approximately 6.3:1, F3666, Bio-serv, NJ). Percentage calories provided by each nutrient category are ...
-
No products found
because this supplier's products are not listed.
Franziska Blaeschke, et al.,
bioRxiv - Immunology 2022
Quote:
... T cells were incubated with NY-ESO-1 specific dextramer (Immudex) for 12 min at RT (1:50 dilution) ...
-
No products found
because this supplier's products are not listed.
Annelien Morlion, et al.,
bioRxiv - Genomics 2021
Quote:
... gDNA heat-and-run removal was performed by adding 1 μl HL-dsDNase (ArcticZymes #70800-202, 2 U/μl) and 0.68 μl reaction buffer (ArcticZymes #66001 ...
-
No products found
because this supplier's products are not listed.
Marta Bermejo-Jambrina, et al.,
bioRxiv - Immunology 2023
Quote:
... SARS-CoV-2 pseudovirus productions were quantified by RETRO-TEK HIV-1 p24 ELISA according to manufacturer instructions (ZeptoMetrix Corporation).
-
No products found
because this supplier's products are not listed.
Jutamas Uttagomol, et al.,
bioRxiv - Cell Biology 2019
Quote:
... cells were plated and grown for 1∼2 days on collagen-coated BioFlex 6-well culture plates with flexible silicone elastomer bottoms (BF-3001C, Flexcell® International Corporation). Each plate was placed over the loading station containing 6 planar faced posts ...
-
No products found
because this supplier's products are not listed.
Gabriela F. Paredes, et al.,
bioRxiv - Microbiology 2021
Quote:
... and single worms of similar size (1-2 mm length, representing adult L. oneistus) were handpicked by forceps (Dumont 3, Fine Science Tools, Canada) under a dissecting microscope ...
-
No products found
because this supplier's products are not listed.
Bee Hui Liu, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... varying concentrations of compounds 1-80 were added to cells with multichannel electronic pipettes (Rainin). Cells were then incubated for 72 hrs at 37°C in a humidified atmosphere of 5% CO2 before 10 μl of CellTiter-Glo reagent was added to the wells with the MultiFlo Microplate Dispenser (BioTek) ...
-
No products found
because this supplier's products are not listed.
Lisa Maria Metz, et al.,
bioRxiv - Cell Biology 2022
Quote:
... For quantification of Panx1 plasma concentration in AAA patients a human Panx1 ELISA (#39097, Signalway Antibody) was performed ...
-
No products found
because this supplier's products are not listed.
Lijun Cong, et al.,
bioRxiv - Microbiology 2021
Quote:
... prior to cell supernatants being collected for quantification of virus production by HIV-1 p24 ELISA (XpressBio).
-
No products found
because this supplier's products are not listed.
Chiara Aloise, et al.,
bioRxiv - Microbiology 2023
Quote:
... The cells were then incubated in blocking buffer containing mouse anti-dsRNA (1:1000; English & Scientific Consulting), goat anti-eIF3η (1:200 ...
-
No products found
because this supplier's products are not listed.
Marco Losa, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... were dispensed at various volumes into human ASC-coated 1,536-well plates using contactless dispensing with an ECHO 555 Acoustic Dispenser (Labcyte). Thereby ...
-
No products found
because this supplier's products are not listed.
Shunsuke Kataoka, et al.,
bioRxiv - Immunology 2021
Quote:
... and then pulsed with 2 μg/ml staphylococcal enterotoxin E (SEE) (Toxin Technology #ET404) for 1hr at 37°C ...
-
No products found
because this supplier's products are not listed.
Laurent Jacob, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Rabbit anti–mouse LYVE-1 (11-034, AngioBio, 1:800), Goat anti– human PROX1 (AF2727 ...
-
No products found
because this supplier's products are not listed.
Liz M. Florez, et al.,
bioRxiv - Plant Biology 2020
Quote:
... PCR amplification products were visualised following gel electrophoresis in a 2% (w/v) agarose gel in 1x Tris Acetate-EDTA (TAE) buffer with RedSafe™ (Intron Biotechnology, SEL, Korea), at the manufacturer’s recommended concentration ...