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No products found
because this supplier's products are not listed.
Hiroki Hayashi, et al.,
bioRxiv - Immunology 2020
Quote:
... and then human ACE2 recombinant protein (0.25 μg/ml, His tag; Sino Biological) was added to the wells and incubated for 2 h at RT ...
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No products found
because this supplier's products are not listed.
Changrui Lu, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... 100 µL biotinylated recombinant human ACE2 protein (His AVI Tag, 0.25 µg/µL, 1:100) (Sinobiology) and recombinant streptavidin protein (phycoerythrin) (Abcam) were used as the primary and secondary antibodies for ACE-2 protein detection ...
-
No products found
because this supplier's products are not listed.
Stephanie Frohn, et al.,
bioRxiv - Plant Biology 2023
Quote:
... The recombinant AtCSD1 protein containing a 6xHis-tag was purified using Dynabeads His-Tag (Thermo Fisher Scientific) according to manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Jacques Augenstreich, et al.,
bioRxiv - Cell Biology 2024
Quote:
... Recombinant human Interferon Gamma (R&D Systems), OADC supplement (R&D Systems) ...
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No products found
because this supplier's products are not listed.
Jacob R. Hambrook, Patrick C. Hanington,
bioRxiv - Immunology 2022
Quote:
... the 6x HIS tag region of the recombinant protein was removed following the vector manufacturers specifications (Novagen). This recombinant SmCI-1 was used to generate a rabbit anti-Sm-CI-1 polyclonal antibody using the Custom pAb service offered by Genscript.
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No products found
because this supplier's products are not listed.
Jinchao Chen, et al.,
bioRxiv - Plant Biology 2023
Quote:
... Recombinant proteins were purified using the His-tag Protein Purification Kit (Ni-NTA Agarose, QIAGEN GmbH), GST-tag Protein Purification Kit (Glutathione Sepharose ...
-
No products found
because this supplier's products are not listed.
Irepan Salvador-Martínez, et al.,
bioRxiv - Immunology 2023
Quote:
... 20 ng/mL recombinant human interferon-gamma (Peprotech, 300-02-20), 10 ng/mL lipopolysaccharides from Escherichia coli O127:B8 (Sigma Aldrich ...
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No products found
because this supplier's products are not listed.
Simona Selberg, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Recombinant human FTO protein was labeled with His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies GmbH; MO-L008). The labelled FTO protein (target ...
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No products found
because this supplier's products are not listed.
Adel Avetisyan, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... His-tagged proteins in soluble fraction were purified using cOmplete His-Tag Purification Columns (Roche). The columns were washed with 10 column volumes of wash buffer 1 (20 mM Tris ...
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No products found
because this supplier's products are not listed.
Giulio Giuliani, et al.,
bioRxiv - Cell Biology 2020
Quote:
... and rabbit anti-PPAR-gamma 1:200 (Cell Signaling 2443S). The following day section were washed three times for 10 minutes with PBS 0.1% Triton X-100 ...
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No products found
because this supplier's products are not listed.
Thomas Fabre, et al.,
bioRxiv - Immunology 2022
Quote:
... Recombinant human interferon gamma (IFNγ; Biolegend) and recombinant human transforming growth factor beta-1 (TGF-β1 ...
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No products found
because this supplier's products are not listed.
Beata M. Walter, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... The CobB protein was separated from His tag on His-Trap column (GE Healthcare), concentrated at 4 °C to 6 mg ml-1 ...
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No products found
because this supplier's products are not listed.
Binhan Hao, Wenjie Zhou, Steven M. Theg,
bioRxiv - Biochemistry 2022
Quote:
... anti-His tag (Genscript) or anti-FLAG (Genscript ...
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No products found
because this supplier's products are not listed.
Anna Balcerak, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Purified HAX1 protein (Proteintech, Ag27244, fused with His-tag) was labeled with RED-NHS 2nd Generation dye according to the supplied labeling protocol Monolith NT™ Protein Labeling Kit ...
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No products found
because this supplier's products are not listed.
Stefania Capone, et al.,
bioRxiv - Immunology 2020
Quote:
... recombinant human His-tagged ACE-2 protein (RayBiotech) was incubated on cells before adding any antibodies ...
-
No products found
because this supplier's products are not listed.
Takumi Takeda, et al.,
bioRxiv - Plant Biology 2022
Quote:
... Purification of His-tagged proteins was carried out using His-tag affinity resin (His-resin) (Clontech, CA, USA).
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No products found
because this supplier's products are not listed.
Veronica H. Ryan, et al.,
bioRxiv - Biochemistry 2020
Quote:
... insoluble His-tag purification (Addgene ID ...
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No products found
because this supplier's products are not listed.
Kazuhiro Yamamoto, et al.,
bioRxiv - Cell Biology 2022
Quote:
... recombinant human HMGB2 (C-terminal His tag, TP720732) from ORIGENE (Rockville, MD); anti-rabbit alkaline phosphatase-linked antibody ...
-
No products found
because this supplier's products are not listed.
Chaelin Lee-Eom, et al.,
bioRxiv - Biophysics 2024
Quote:
Full-length human tau protein with a 6x His-tag was cloned into the pRK172 vector and expressed in LEMO21 cells (NEB #C2528J). Cells were cultured in TB media at 37 °C until the OD600 reached 0.6 ...
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No products found
because this supplier's products are not listed.
Sandro Trova, et al.,
bioRxiv - Microbiology 2023
Quote:
... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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No products found
because this supplier's products are not listed.
Deepti Mudaliar, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Recombinant Human CKII alpha prime polypeptide His Protein (NBP2-22736) was purchased from Novus Biologicals (Centennial, CO, USA). CK2 Substrate (synthetic peptide RRRADDSDDDDD ...
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No products found
because this supplier's products are not listed.
Oanh T.P. Nguyen, et al.,
bioRxiv - Immunology 2024
Quote:
... the cells were adjusted to 7.5×105 cells/mL and stimulated with recombinant human interferon gamma (IFNγ, cat. no. PHP050, BioRad) at 0.2 μg/mL for 3 h at 37°C to activate the phagocytic ability of MonoMac-6.10 Meanwhile ...
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No products found
because this supplier's products are not listed.
Steven Swendeman, et al.,
bioRxiv - Biochemistry 2022
Quote:
... and His-tag (Santa Cruz) expression (data not shown) ...
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No products found
because this supplier's products are not listed.
Katarzyna Olga Rojek, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Human recombinant VEGF-165 (Stemcell technologies; Saint Egrève ...
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No products found
because this supplier's products are not listed.
Simone Giaveri, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... His-tag proteins have been purified by using MagneHis system (Promega) and DynaMag spin magnet (Thermo Fisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Wenjuan Yang, et al.,
bioRxiv - Immunology 2021
Quote:
... human recombinant IL-2 (Miltenyi Biotec) was added to the medium every two days from day 2 on (100 U/ml) ...
-
No products found
because this supplier's products are not listed.
Lie Wang, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Purified hDGAT1 (with his tag) was absorbed onto Copper HIS-Tag PVT beads (Perkin Elmer, RPNQ0095) and incubated with [3H]-Acetyl-CoA (ARC ...
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No products found
because this supplier's products are not listed.
Mireia Perez Verdaguer, et al.,
bioRxiv - Systems Biology 2022
Quote:
Recombinant human EGF was purchased from BD Biosciences (San Jose ...
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No products found
because this supplier's products are not listed.
Farah Haque, et al.,
bioRxiv - Cell Biology 2021
Quote:
... The mGli2-His recombinant protein was expressed in BL21 (DE3) pLysS cells (Stratagene) and purified under denaturing condition (8 M urea ...
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No products found
because this supplier's products are not listed.
Shiho Tanaka, et al.,
bioRxiv - Immunology 2021
Quote:
... recombinant SARS-CoV-2 S2-His-tag at 10 µg/mL was loaded on Anti-Penta-HIS (HIS1K) biosensors (Sartorius Corporation) for 1 min ...
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No products found
because this supplier's products are not listed.
M. Eugenia Dieterle, et al.,
bioRxiv - Microbiology 2020
Quote:
... Human gamma globulin (009-000-002) purchased from Jackson ImmunoResearch was used as negative control.
-
No products found
because this supplier's products are not listed.
Nur F. Isa, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Jinkai Zang, et al.,
bioRxiv - Microbiology 2020
Quote:
... recombinant RBD protein containing a C-terminal 6xHis tag was formulated with the Alhydrogel adjuvant (Invivogen) and each vaccine dose contained 5 μg of RBD and 500 μg of aluminum hydroxide ...
-
No products found
because this supplier's products are not listed.
Ross Bacchetti, et al.,
bioRxiv - Microbiology 2024
Quote:
IFN-gamma KO mice (Jackson Laboratory #B6.129S7-IfngtmlTS/J ...
-
No products found
because this supplier's products are not listed.
Olena Parilova, Piia Bartos, Anssi M. Malinen,
bioRxiv - Biochemistry 2024
Quote:
... Fractions and final Rrn3 preparations were quality controlled by SDS-PAGE and Western analysis using Coomassie staining and monoclonal anti-His-tag antibody ( 6X His Tag Antibody Dylight™ 800 Conjugated, clone 33D10.D2, cat.no. 200-345-382, Rockland Immunochemicals), respectively.
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No products found
because this supplier's products are not listed.
Ines A Cadena, et al.,
bioRxiv - Bioengineering 2024
Quote:
... and recombinant human insulin 0.5%) (Lonza). Human cervical cancer cell line SiHa from ATCC (ATCC® HTB-35™ ...
-
No products found
because this supplier's products are not listed.
Cameron Geller, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... human recombinant EGF (Corning, Bedford, MA), hydrocortisone (BD Biosciences ...
-
No products found
because this supplier's products are not listed.
M. T. Gyparaki, et al.,
bioRxiv - Cell Biology 2020
Quote:
... recombinant binding protein (gt-250, Chromotek). The secondary antibodies we used were AffiniPure goat anti-mouse IgG (H+L ...
-
No products found
because this supplier's products are not listed.
Carolyn Rydyznski Moderbacher, et al.,
bioRxiv - Immunology 2022
Quote:
... the hACE2 receptor bound to the S protein was then detected using a mouse anti-His-Tag horseradish peroxidase conjugate (Southern Biotech) and a colorimetric signal generated by the addition of 3,3′,5,5′-TMB substrate ...
-
No products found
because this supplier's products are not listed.
Shraddha Pai, et al.,
bioRxiv - Genomics 2021
Quote:
We downloaded publicly-available Hi-C data from human prefrontal cortex tissue23,24 (Illumina HiSeq 2000 paired-end raw sequence reads ...
-
No products found
because this supplier's products are not listed.
Hisashi Nomura, et al.,
bioRxiv - Immunology 2021
Quote:
... cells were incubated with 10 μg of rDsg3-E-His in 100 μL of RPMI on ice for 1 h and stained with anti-E-tag-FITC (Bethyl Laboratories). After excluding dead cells by staining with 7AAD ...
-
No products found
because this supplier's products are not listed.
Alexander W. Justin, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Green fluorescent protein (GFP) and Red Fluorescent Protein (RFP) human umbilical vein endothelial cells (HUVECs, Promocell), normal human lung fibroblasts (NHLFs ...
-
No products found
because this supplier's products are not listed.
Xiaoning Yang, et al.,
bioRxiv - Immunology 2023
Quote:
Recombinant human PD-1 protein was biotinylated and loaded using SA sensor (Pall Corporation). Six concentrations of Finotonlimab and Nivolumab were added for real-time association and dissociation analysis using Octet system ...
-
No products found
because this supplier's products are not listed.
A.A. Shaldzhyan, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... one milliliter of NS1 recombinant protein (3 mg/ml in 2x PBS) was placed into a 1.5 ml Eppendorf tube (Eppendorf, Germany); and the tube mixed for 66 hours (55°C ...
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No products found
because this supplier's products are not listed.
Therese de Neergaard, et al.,
bioRxiv - Immunology 2022
Quote:
Human monocytic cell line Tamm–Horsfall protein 1 (THP-1) (TIB-202, male; American Type Culture Collection) was cultured in RPMI 1640 medium (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Vivian K Lee, et al.,
bioRxiv - Bioengineering 2024
Quote:
... Human astrocytes (Sciencell) and human brain vascular pericytes (Sciencell ...
-
No products found
because this supplier's products are not listed.
Andrew M. Baschnagel, et al.,
bioRxiv - Cancer Biology 2020
Quote:
Immunodeficient NOD SCID gamma (NSG) (Envigo, Indianapolis) and Hsd:athymic Nude-Foxn1nu (Envigo ...
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No products found
because this supplier's products are not listed.
Teddy Kamata, Chun-Song Yang, Bryce M. Paschal,
bioRxiv - Biochemistry 2021
Quote:
... recombinant GST-tagged tandem AF1521 was fluorescently labeled using IRDye® 800CW Protein Labeling Kit (LI-COR Biosciences, Lincoln, NE, USA). Prepared samples were run on SDS-PAGE ...
-
No products found
because this supplier's products are not listed.
Camilla Kjeldgaard Larsen, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... The cleaved proteins were incubated with Ni-NTA beads for revers his-purification and cleaved proteins were collected in the flow through and visualized on SDS-PAGE (Mini Protean Tris-Tricine Precast gels, BioRad).
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No products found
because this supplier's products are not listed.
Sanjib Saha, et al.,
bioRxiv - Cell Biology 2024
Quote:
... Protein expression was normalized to total protein (DM-TP01, Protein Simple) and compared between tissue and cell types.