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No products found
because this supplier's products are not listed.
Anne-Emmanuelle Foucher, et al.,
bioRxiv - Biochemistry 2022
Quote:
... The aligned sample contained 17 mg/mL Pf1 phage (Asla Biotech). RDC-based intervector projection angle restraints used Da and R values of 8 and 0.33 ...
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No products found
because this supplier's products are not listed.
Doris Krauter, et al.,
bioRxiv - Neuroscience 2021
Quote:
... using a diamond knife (Histo HI 4317, Diatome). Afterwards sections were stained according to Gallyas52 and with Methylene blue/ Azur II for 1 min ...
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No products found
because this supplier's products are not listed.
Stacia M. Nicholson, Francis A.X. Schanne,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
Recombinant mouse TNFSF11 (IBI Scientific, Indiana), also known as RANKL ...
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No products found
because this supplier's products are not listed.
Chafen Lu, et al.,
bioRxiv - Microbiology 2019
Quote:
... Secondary antibodies were rabbit polyclonal anti-His (Delta Biolabs), Horseradish peroxidase (HRP)-anti-rabbit and HRP-anti-mouse IgG (GE Healthcare ...
-
No products found
because this supplier's products are not listed.
Qian Shao, et al.,
bioRxiv - Biochemistry 2023
Quote:
Estrogen Receptor Luciferase Reporter T47D Stable Cell Line (obtained from Signosis, SL-0002) was cultured in RPMI-1640 containing 10% (v/v ...
-
No products found
because this supplier's products are not listed.
Catherine N. Russell, et al.,
bioRxiv - Biochemistry 2023
Quote:
... The sample was lysed by French press at 17 KPsi (Constant Systems Ltd), and any remaining sample washed with 15 ml lysis buffer ...
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No products found
because this supplier's products are not listed.
Lindsey R. Conroy, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Recombinant PNGaseF Prime was obtained from Bulldog Bio, Inc ...
-
No products found
because this supplier's products are not listed.
Isaac R. Cinco, et al.,
bioRxiv - Immunology 2023
Quote:
... Goat anti-monkey IgG (Fc) HRP (Brookwood Biomedical, Jemison, AL) was diluted then added to each well and incubated for 1-1.5 hr ...
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No products found
because this supplier's products are not listed.
Jinye Liang, et al.,
bioRxiv - Microbiology 2023
Quote:
... and imaged on a Li-COR Odyssey Fc scanner (Carver Center for Genomics ...
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No products found
because this supplier's products are not listed.
Joshua J. Kellogg, et al.,
bioRxiv - Microbiology 2023
Quote:
... 17 mL of culture was added to 20 mL serum bottles (Wheaton, actual volume 28 mL). Bottles were sealed using a vial crimper with rubber caps (Wheaton ...
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No products found
because this supplier's products are not listed.
Camden R. Bair, et al.,
bioRxiv - Microbiology 2021
Quote:
... supplemented with 8% heat-inactivated newborn calf serum (HI-NBCS, Rocky Mountain Biologicals), 1.5 g/ml Na2CO3 (Amresco) ...
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No products found
because this supplier's products are not listed.
Angela Kim, et al.,
bioRxiv - Cell Biology 2021
Quote:
... was from Tocris (Bio-Techne Ltd, UK). The glucagon receptor antagonist LY2409021 (adomeglivant; Kazda, et al. (30)) was from MedKoo Biosciences (USA).
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No products found
because this supplier's products are not listed.
Marta Fontcuberta-PiSunyer, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... Human insulin was measured using a human Insulin ELISA kit (Crystal Chem, Zaandam, Netherlands) and C-peptide using a human C-peptide ELISA kit (Mercodia ...
-
No products found
because this supplier's products are not listed.
Hsiao-Yun Chen, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... then embedded/inserted into paraffin recipient block/negative mold (IHC World; 10*17 Quick Ray mold IW-UM01-1). Empty slots were filled with blank paraffin cores ...
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No products found
because this supplier's products are not listed.
David Rozema, Paul Lasko, François Fagotto,
bioRxiv - Developmental Biology 2023
Quote:
... Cells were manipulated with custom made pipettes with diameters from 13-17 μm with a 15° bend (Sutter Instruments) that were coated with BSA ...
-
No products found
because this supplier's products are not listed.
David Levcik, et al.,
bioRxiv - Neuroscience 2021
Quote:
... and a customized microdrive with 2 × 4 stereotrode arrays (17 μm Ni-Chrome wire with insulation; A-M Systems, USA) was chronically implanted unilaterally above NAc shell at the following coordinates ...
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No products found
because this supplier's products are not listed.
Matthew Dawson, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Blue light (470 nm, 17 mW) was delivered continuously via ANY-Maze software-controlled AMi-2 optogenetic interface (Stoelting Co.) connected to the laser driver.
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No products found
because this supplier's products are not listed.
Tsuyoshi Hattori, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The neurons were cultured with or without SPARCL1 recombinant protein (80 nM, ATGP3891, NKMAX) from day 8 to day 14 as previously done (34) ...
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No products found
because this supplier's products are not listed.
Jared D. Chrispell, Yubin Xiong, Ellen R. Weiss,
bioRxiv - Cell Biology 2022
Quote:
... Rabbit polyclonal antibodies against zebrafish Grk7 (27) and phosphorylated mouse Grk1 (17) were generated by 21st Century Biochemicals (Marlboro, MA, USA). A novel rabbit polyclonal antibody against phosphorylated zebrafish GRK1 was also generated by 21st Century Biochemicals using the peptide ISARG[pS]FDGTAN corresponding to amino acids 16-27 of zebrafish Grk1a ...
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No products found
because this supplier's products are not listed.
Ina J. Andresen, et al.,
bioRxiv - Cell Biology 2020
Quote:
... RNA from 3 batches (batch 17, 19 and 25) was isolated using the “Single Cell RNA Purification Kit” (NORGEN BIOTEK CORP, Canada) with an 8 ul elution buffer ...
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No products found
because this supplier's products are not listed.
Erin J. Vanzyl, et al.,
bioRxiv - Cell Biology 2020
Quote:
... ATF3 mRNA expression was determined using Bioline SensiFAST™ Probe HI-ROX Master Mix (FroggaBio Inc, Toronto, ON) with SYBR green ...
-
No products found
because this supplier's products are not listed.
Yunye Zhu, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Transformants scraped from densely plated transformation plates were inoculated into fresh SC-His medium with 2% raffinose (Amresco, J392) at 0.25 x 107 cells/ml and grown until 0.5-0.8 x 107 cells/ml ...
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No products found
because this supplier's products are not listed.
Young Sun Hwang, M. Andrés Blanco, Kotaro Sasaki,
bioRxiv - Developmental Biology 2022
Quote:
... hiPSCs were cultured on plates coated with recombinant laminin-511 E8 (iMatrix-511 Silk, Nacalai USA) and maintained under feeder-free conditions in StemFit® Basic04 medium (Ajinomoto ...
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No products found
because this supplier's products are not listed.
Elodie Darbo, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... FISH assay was performed using a commercially available MYOCD FISH probe labeled in spectrum orange and a chromosome 17 control probe labeled in FITC (EG-MYOCD-CHR17-20ORGR, Empire Genomics, Williamsville, NY, USA). MYOCD and control probe enumeration was performed with a Nikon Eclipse 90i fluorescent microscope with appropriate filters ...
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No products found
because this supplier's products are not listed.
Darko Bosnakovski, et al.,
bioRxiv - Molecular Biology 2023
Quote:
Control LHCN-M2 immortalized human myoblasts 35 and FSHD (M008) immortalized human myoblasts were cultured in F10 medium (HyClone) with 20% FBS (Peak Serum), 2-mercaptoethanol 1x (Gibco) ...
-
No products found
because this supplier's products are not listed.
Cunxi Wang, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... The Mpp75Aa1.1-His fractions identified by SDS-PAGE were pooled and further processed using EndoTrap® HD (Hyglos GmbH, Germany) to reduce endotoxin contents (manufacturer’s instruction) ...
-
No products found
because this supplier's products are not listed.
Amanda L. Photenhauer, et al.,
bioRxiv - Molecular Biology 2022
Quote:
Custom α-Sas6T antiserum was generated by rabbit immunization with purified recombinant Sas6T protein (Lampire Biological Laboratories). The resulting antiserum was used for western blotting and cell staining ...
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No products found
because this supplier's products are not listed.
Carlos J Nogueras-Ortiz, et al.,
bioRxiv - Neuroscience 2020
Quote:
... or anti-human tetraspanin CD81 (Ancell Corporation, Bayport, MN) biotinylated antibodies ...
-
No products found
because this supplier's products are not listed.
Vaibhav Sidarala, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Anti-human TFAM (1:1000, PhosphoSolutions, Catalog# 1999-hTFAM), Anti-mouse TFAM (1:1000 ...
-
No products found
because this supplier's products are not listed.
Yunlong Li, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... the full-length codon optimized gene with N-terminal 6x-His-sequnce and NdeI-XhoI sites was synthesized (IDT DNA Inc.) (Table S2) ...
-
No products found
because this supplier's products are not listed.
Shahrnaz Kemal, et al.,
bioRxiv - Neuroscience 2023
Quote:
... All vectors contain an N-terminal 6x-His-tag, C terminal Strep-Tag II tag, with or without a N or C terminal fluorophore (EGFP, mNeonGreen (Allele Biotech), or mRuby2).
-
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Glory Nasseri, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Human PRG-1 obtained from DNASU Plasmid Repository (The Biodesign Institute/Arizona State University ...
-
No products found
because this supplier's products are not listed.
Livia Mazzini, et al.,
bioRxiv - Immunology 2020
Quote:
ELISA plates were coated with 1µg/mL of purified recombinant Spike S1 Protein (aa 18-676) (eEnzyme, Gaithersburg, MD, USA) or with 1µg/mL Spike-RBD (Arg319-Phe541 ...
-
No products found
because this supplier's products are not listed.
Masaya Matsubayashi, et al.,
bioRxiv - Biochemistry 2019
Quote:
The human hepatocellular carcinoma cell HepG2 was purchased from Cellular Engineering Technologies ...
-
No products found
because this supplier's products are not listed.
Satoshi Watanabe, et al.,
bioRxiv - Biochemistry 2023
Quote:
... The established stable cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Nacalai Tesque) supplemented with 4∼5% fetal calf serum (FCS; Thermo Fisher Scientific, or Nichirei Biosciences Inc) and 1% penicillin-streptomycin mixed solution (Nacalai Tesque ...
-
No products found
because this supplier's products are not listed.
Kyle E. Landgraf, et al.,
bioRxiv - Cancer Biology 2019
Quote:
Human PBMC stimulation and immune-phenotyping studies were performed by iQ Biosciences. Briefly normal PBMCs from three donors were seeded in 96-well plates at 1×105 cells/well and exposed to a 10-fold dilution series of either U2S3-hFc-mutIL2 or U2S3-hFc-wtIL2 (wild-type IL2 ...
-
No products found
because this supplier's products are not listed.
Eli Fritz McDonald, et al.,
bioRxiv - Cell Biology 2021
Quote:
... purified from B lymphocyte hybridoma cells using a Recombinant Protein G - Sepharose 4B Affinity Column on an ÄKTA start protein purification system (GE Life Science Product # 29022094). Antibodies were used in immunoblotting buffer (5% bovine serum albumin (BSA ...
-
No products found
because this supplier's products are not listed.
Alberto Domingo López-Muñoz, et al.,
bioRxiv - Microbiology 2023
Quote:
... alone or in combination with purified recombinant proteins were placed in the lower chamber of a 96-well ChemoTx System plate (Neuro Probe # 101-5) in RPMI 1640 1 % FBS ...
-
No products found
because this supplier's products are not listed.
Tomoyuki Ohno, et al.,
bioRxiv - Bioengineering 2022
Quote:
... 201B7 human iPS (RIKEN BRC, HPS0063) cells were maintained in StemFit AK02N medium (ReproCELL) in dishes coated with iMatrix-511 (Nippi) ...
-
No products found
because this supplier's products are not listed.
Vincent Mouilleau, et al.,
bioRxiv - Developmental Biology 2020
Quote:
Human SA001 embryonic stem cell (ESC) line (male, RRID: CVCL_B347) was obtained from Cellectis and used accordingly to the French current legislation (Agency of Biomedicine ...
-
No products found
because this supplier's products are not listed.
Matthew J. Shannon, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... The assay was validated using a standard curve calibrated against human methylation controls (EpigenDx, USA Cat ...
-
No products found
because this supplier's products are not listed.
Aereas Aung, et al.,
bioRxiv - Immunology 2021
Quote:
... slides were stained with human VRC01 mAb directly labeled with AF488 NHS ester (21820, Lumiprobe). For non-permeabilized sections ...
-
No products found
because this supplier's products are not listed.
Delphine Planas, et al.,
bioRxiv - Immunology 2021
Quote:
... 2) a CE-Marked ELISA assay for detection of IgG against the full-length recombinant N protein from Epitope Diagnostics (EDITM Novel coronavirus COVID-19 IgG). The test has a specificity of 96% and a sensitivity of 81% after 28 days POS in our hands ...
-
No products found
because this supplier's products are not listed.
Stephen A. Schumacher, et al.,
bioRxiv - Physiology 2021
Quote:
Serum PTH concentrations were measured with a human-specific immunoradiometric assay (Scantibodies Laboratory, Santee, CA, USA) with a working range of 6.5-2328 pg/mL ...
-
No products found
because this supplier's products are not listed.
Benjamin D. Hobson, et al.,
bioRxiv - Neuroscience 2022
Quote:
... A small hole was drilled into the skull and 400 nL of 0.9% saline or 0.9% saline + 100 ng/μL recombinant mouse IFN-γ (40 ng IFN-γ total) was injected through a pulled glass pipet using a Nanoject 2000 (Drummond Scientific; 8 pulses of 50 nL). At 5 minutes after injection ...
-
No products found
because this supplier's products are not listed.
Xin Wang, et al.,
bioRxiv - Immunology 2022
Quote:
Mouse trachea and human ALI culture samples were harvested and fixed with 4% PFA (BM-155, Boston BioProducts) overnight at 4 °C ...
-
No products found
because this supplier's products are not listed.
Michal Schwartz, et al.,
bioRxiv - Microbiology 2022
Quote:
... using FAM labeled HCMV primer and probe: Human CMV HHV5 kit for qPCR using a glycoprotein B target (PrimerDesign); and HEX labeled RPP30 copy number assay for ddPCR (Bio-Rad) ...
-
No products found
because this supplier's products are not listed.
Marco Losa, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... were dispensed at various volumes into human ASC-coated 1,536-well plates using contactless dispensing with an ECHO 555 Acoustic Dispenser (Labcyte). Thereby ...
-
No products found
because this supplier's products are not listed.
Kyung-Jin Jang, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
Alpha Glutathione S-Transferase (α-GST): levels were quantified in human model effluent samples from the upper channel using an ELISA kit (DiaPharma). The assay was run following the vendor protocol using a standard curve ranging from 0-64 µg/L ...
-
No products found
because this supplier's products are not listed.
Ram Sagar, et al.,
bioRxiv - Genetics 2023
Quote:
To acquire a high yield of functional neurons we transduced the generated human iPSCs with Ngn2 and rTTA expressing lentivirus (lentivirus was purchased by Cellomics Technology, Maryland USA). We followed an established protocol for generation of induced neuronal cells in 21 days 17 ...