-
No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
His-tagged SSRP1 was PCR amplified from SSRP1 pReceiver (GeneCopoeia) using primers encoding an N-terminal His tag and NdeI/NotI cut sites for cloning into pET22b ...
-
No products found
because this supplier's products are not listed.
Shraddha Pai, et al.,
bioRxiv - Genomics 2021
Quote:
We downloaded publicly-available Hi-C data from human prefrontal cortex tissue23,24 (Illumina HiSeq 2000 paired-end raw sequence reads ...
-
No products found
because this supplier's products are not listed.
Tal Noy-Porat, et al.,
bioRxiv - Immunology 2021
Quote:
... ELISA of both sera and recombinant human antibodies was applied with AP-conjugated Donkey anti-human IgG (Jackson ImmunoResearch, USA ...
-
No products found
because this supplier's products are not listed.
Chien-Wei Wang, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Recombinant His-tagged streptavidin (Fitzgerald Industries, Acton, MA) at a concentration of 0.75 μM was used as a negative control molecule ...
-
No products found
because this supplier's products are not listed.
Josephine W. Thinwa, et al.,
bioRxiv - Microbiology 2022
Quote:
... was combined with 3 μg of recombinant human SQSTM1/p62 protein (GeneTex, GTX68012-pro) in buffer containing 20 mM HEPES (pH 7.5 ...
-
No products found
because this supplier's products are not listed.
Hourinaz Behesti, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... 7 μg/ml human recombinant Insulin (Tocris), 100 ng/ml recombinant human/mouse FGF8b (Tocris ...
-
No products found
because this supplier's products are not listed.
Jun-Gu Kang, et al.,
bioRxiv - Microbiology 2019
Quote:
... His-tagged Gn and Gc recombinant proteins were purchased from Immune Technology Co ...
-
No products found
because this supplier's products are not listed.
Aleksei Kuznetsov, et al.,
bioRxiv - Biochemistry 2020
Quote:
Human recombinant ACE2-His protein (Icosagen OÜ, Estonia, cat# P-302-100) and SARS-CoV-2 Spike protein S1 (Icosagen OÜ ...
-
No products found
because this supplier's products are not listed.
Ida Lindeman, et al.,
bioRxiv - Immunology 2023
Quote:
... 3 µg/ml recombinant human TG2 or 2 µg/ml goat anti-human Ig (Southern Biotech) was coated in PBS followed by incubation with culture supernatants diluted in PBS supplemented with 0.1% (v/v ...
-
No products found
because this supplier's products are not listed.
Mariska S. Simpson, et al.,
bioRxiv - Cell Biology 2023
Quote:
... His-tagged proteins were affinity purified by binding to cobalt resin (GoldBio) equilibrated in 1X PBS ...
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No products found
Katharina M. Eyme, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... human recombinant EGF (20 ng/mL; ABM), and human recombinant bFGF-2 (10ng/mL ...
-
No products found
because this supplier's products are not listed.
Jun Weng, et al.,
bioRxiv - Biochemistry 2021
Quote:
Binding of nucleobases was performed by means of the scintillation proximity assay (SPA) using His-tagged PurTCp in conjunction with Copper HIS-Tag YSI beads (Perkin Elmer, RPNQ0096). 250 ng of purified PurTCp were immobilized on 125 μg SPA beads per 100-μL assay in 200 mM Tris/Mes at the indicated pH ...
-
No products found
because this supplier's products are not listed.
Kevin Schmidt, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... PenStrep as well as 1 ng/mL human recombinant basic FGF and 5 ng/mL Insulin (both from Growth Medium 3 SupplementPack, C-39350, PromoCell). HEK293FT and HUVECs were kept in T75 flasks (Sarstedt ...
-
No products found
because this supplier's products are not listed.
Tommy Stormberg, Yuri L. Lyubchenko,
bioRxiv - Molecular Biology 2022
Quote:
... recombinant human octamers containing H3 were purchased from EpiCypher. Nucleosome assembly was achieved using the continuous dilution method ...
-
No products found
because this supplier's products are not listed.
Stefanie S. Schalm, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and glypican 3 (Cell Marque, #261M) was detected using BOND Polymer Refine Detection (Leica Biosystems, #DS9800), including a hematoxylin counterstain to visualize nuclei.
-
No products found
because this supplier's products are not listed.
Priyabrata Mohanty, et al.,
bioRxiv - Microbiology 2021
Quote:
... 50 μL mouse monoclonal anti-6X His tagged primary antibody (Calbiochem) was added at a dilution of 1:150 in 0.5 % BSA in PBST in the respective wells and incubated for 2 hours without shaking at room temperature ...
-
No products found
because this supplier's products are not listed.
Benjamin N. Bell, et al.,
bioRxiv - Immunology 2021
Quote:
His-tagged SARS-CoV-2 RBD (300 nM) was loaded on His1K biosensors (Pall ForteBio) to a load threshold of 0.4-0.6 nm ...
-
No products found
because this supplier's products are not listed.
Darrell Pilling, et al.,
bioRxiv - Immunology 2022
Quote:
... and c-Myc–tagged recombinant mouse NEU3 was purified using a Myc-Trap agarose kit (ytak-20; Chromotek, Hauppauge, NY) following the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Ana F. Ferreira, et al.,
bioRxiv - Neuroscience 2023
Quote:
Human samples: qPCR experiments were conducted using the SensiFAST Probe Hi-ROX Master Mix (Bioline) and the TaqMan Gene Expression Assays (Applied Biosystems ...
-
No products found
because this supplier's products are not listed.
Joseph Atherton, et al.,
bioRxiv - Biochemistry 2020
Quote:
... HA-tagged constructs and bioGFP-tagged constructs using MaxPEI (Polysciences) in a ratio of 3/1 PEI/DNA ...
-
No products found
because this supplier's products are not listed.
Chong Li, et al.,
bioRxiv - Genomics 2023
Quote:
... Hi-C libraries were generated with 1.5 M human cells as input using Proximo Hi-C kits v4.0 (Phase Genomics, Seattle, WA) according to the manufacturer’s protocol with the following change ...
-
No products found
because this supplier's products are not listed.
Hristina Vasileva, et al.,
bioRxiv - Immunology 2023
Quote:
... the SC and V2 domains were cloned into the pET15b expression vector as dual His (N-terminal) and Myc (C-terminal) tagged recombinant constructs (Biomatik, USA). A duplicate set of expression constructs were also cloned into the pGEX-4T-1 expression vector as single-tagged GST (N-terminal ...
-
No products found
because this supplier's products are not listed.
A-M C Overstreet, et al.,
bioRxiv - Microbiology 2023
Quote:
... coli were made from an overnight agar culture and incubated with 3 mM HMGB1 recombinant protein containing a HIS tag (Abclonal) or an hFc tag (Sino Biological ...
-
No products found
because this supplier's products are not listed.
Yukiko Yamaguchi, et al.,
bioRxiv - Immunology 2022
Quote:
... containing 100 U/mL recombinant human IL-2 (rhIL-2, Novartis Oncology) and 0.5 ng/mL recombinant human IL-15 (rhIL-15, CellGenix). For CAR lentiviral transduction ...
-
No products found
because this supplier's products are not listed.
Kylie M. Konrath, et al.,
bioRxiv - Immunology 2021
Quote:
... Cytiva) for His-tagged monomers and gravity flow columns filled with Agarose bound Galnthus Nivalis Lectin beads (Vector Labs) for nanoparticles ...
-
No products found
because this supplier's products are not listed.
Matthew J. Burke, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Single-cell clones were then assessed for binding to His-tagged SARS-CoV-2 Spike using a Cytoflex S (Beckman) cell analyser ...
-
No products found
because this supplier's products are not listed.
Eliram Nof, et al.,
bioRxiv - Bioengineering 2021
Quote:
Human airway epithelial cell line Calu-3 (American Type Culture Collection, Manassas, VA) was used in this study (passage number 15-20) ...
-
No products found
because this supplier's products are not listed.
Michael G. Spelios, et al.,
bioRxiv - Immunology 2021
Quote:
... purified His-tagged ACE2 (EpiGentek) was added at a concentration of 100 ng/well (prepared with PBS ...
-
No products found
because this supplier's products are not listed.
Yanzhou Zhang, et al.,
bioRxiv - Cell Biology 2021
Quote:
His-tagged or His-sumo-tagged protein was purified using NiNTA beads (Anatrace, Cat. No. SUPER-NINTA25). Briefly ...
-
No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... 5 μg of His-tagged H2A:H2B or His-H3:Flag-H4 (Diagenode) and 2 μg of Flag-hSpt16 were added to 250 μl of binding buffer (50 mM Tris-HCl pH 7.4 ...
-
No products found
because this supplier's products are not listed.
Tomohisa Sebastian Tanabe, et al.,
bioRxiv - Biochemistry 2023
Quote:
... His-tagged and Strep-tagged proteins were purified with Ni-NTA Agarose (Jena Bioscience) (except for AqTuA that was purified with a HiScreen Ni FF column (Cytiva) ...
-
No products found
because this supplier's products are not listed.
Esther A. Mozipo, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Recombinant human bone morphogenetic protein-2 (BMP-2) and the human BMP-2 DuoSet enzyme-linked immunosorbent assay (ELISA ...
-
No products found
because this supplier's products are not listed.
Irina Buckle, et al.,
bioRxiv - Immunology 2022
Quote:
... and 50ng/ml recombinant human IL-18 (MBL International) were added to the IL-15 cultures for the last 12 hrs (overnight stimulation).
-
No products found
because this supplier's products are not listed.
Michael J Abrams, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
... and 0.1 mg/mL insulin (human recombinant, MP Biomedicals 0219390080). To introduce the molecular factors ...
-
No products found
because this supplier's products are not listed.
Caroline Atyeo, et al.,
bioRxiv - Immunology 2021
Quote:
... Avi-Tagged FcRs (Duke Human Vaccine Institute) were biotinylated using BirA500 kit (Avidity) per manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Filippo Vascon, et al.,
bioRxiv - Biochemistry 2024
Quote:
... and TetraCys-tagged LexAPaCTD were amplified from the genomic DNA using primers LexA_Pa.For/Rev and LexA_Pa_CTD_4Cys.For/Rev (Supplementary Table 3) and cloned in pETite C-His Kan vector and pETite N-His SUMO Kan Vector (Lucigen), respectively ...
-
No products found
because this supplier's products are not listed.
Nawar Naseer, et al.,
bioRxiv - Microbiology 2021
Quote:
... and 50 ng/ml recombinant human M-CSF (Gemini Bio-Products) for 6 days to promote differentiation into hMDMs ...
-
No products found
because this supplier's products are not listed.
Liangxi Wang, et al.,
bioRxiv - Genomics 2022
Quote:
... 10 ng/ml recombinant human TNFα (Cell Applications, cat# RP1111-50) was added for 3 hours ...
-
No products found
because this supplier's products are not listed.
Teddy Kamata, Chun-Song Yang, Bryce M. Paschal,
bioRxiv - Biochemistry 2021
Quote:
... recombinant GST-tagged tandem AF1521 was fluorescently labeled using IRDye® 800CW Protein Labeling Kit (LI-COR Biosciences, Lincoln, NE, USA). Prepared samples were run on SDS-PAGE ...
-
No products found
because this supplier's products are not listed.
Jason Wallach, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and a Venus-tagged N-terminal β-arrestin2 using 3:1 ratio of TransiT-2020 (Mirus). 5-HT2A receptor mutants were designed and performed using Q5 mutagenesis kit (New England Biolabs) ...
-
No products found
because this supplier's products are not listed.
Kathryn L. Wofford, et al.,
bioRxiv - Bioengineering 2019
Quote:
... either human recombinant myelin basic protein (Alfa Aesar, cat. #AAJ66051LB0) fluorescently labeled with Vybrant DiD (Molecular Probes ...
-
No products found
because this supplier's products are not listed.
Kanak Raina, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... 20 nM of His-tagged BRD4 BD1 and 20 nM of biotinylated histone H4 K5/8/12/16(Ac) peptide (AnaSpec) were mixed in 20 mM HEPES pH 7.5 ...
-
No products found
because this supplier's products are not listed.
Xingxing Zhu, et al.,
bioRxiv - Immunology 2024
Quote:
... and 20 ng/mL recombinant human BAFF or 5 μg/mL anti-CD40 (Bio X Cell) and 10 ng/mL recombinant mouse IL-4 ...
-
No products found
because this supplier's products are not listed.
Casimir Bamberger, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... 2xStrep tagged SARS-CoV-2 ORFs were immunoprecipitated using 30 μl of Megastrep type 3 XT beads (IBA LifeSciences, Germany), and flag-tagged SARS-CoV-2 ORFs were immunoprecipitated using Anti-DYKDDDDK Magnetic Agarose (Pierce ...
-
No products found
because this supplier's products are not listed.
Mahnaz Shariatzadeh, et al.,
bioRxiv - Immunology 2024
Quote:
... transduced with GFP-tagged lentiviral vector were co-cultured with human brain vascular pericytes (cat # SCC1200-KIT, ScienCell research, San Diego, USA) in a collagen matrix (bovine collagen type-1 ...
-
No products found
because this supplier's products are not listed.
Carole Seguin-Devaux, et al.,
bioRxiv - Immunology 2024
Quote:
... or rabbit anti-His (Bethyl, ImTec Diagnostic NV ...
-
No products found
because this supplier's products are not listed.
Mohammed Repon Khan, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Human alpha-syn(A53T) transgenic G2-3 lines are available from Jackson Laboratory (13) were aged and late symptomatic mice were used for biochemical analysis and isolation of endogenous A53T α-syn aggregates ...
-
No products found
because this supplier's products are not listed.
Pablo Lazcano, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Corresponding europium-tagged secondary antibodies (Molecular Devices) were used at a 1:5000 dilution and 1:20,000 for actin ...
-
No products found
because this supplier's products are not listed.
Steven J. Kunnen, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
... in INVITROGRO HI medium (BioIVT, #Z99009) supplemented with TORPEDO antibiotics mix (BioIVT ...
-
No products found
because this supplier's products are not listed.
Prashant P. Damke, et al.,
bioRxiv - Microbiology 2022
Quote:
... using a microplate reader (BioTek Synergy HI) to record the absorbance at 650 nm ...