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Cat# IT-45-25,
25 micrograms,USD $303.0
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Judith Jing Wen Wong, Pål Kristian Selbo,
bioRxiv - Cancer Biology 2021
Quote:
The immunotoxin anti-human PD-L1-saporin (Anti-PD-L1-SAP, BETA-014) was generously provided by Advanced Targeting Systems (ATS, Carlsbad, CA, USA). The targeted toxin is a 295 kDa conjugate between a rabbit polyclonal antibody to human PD-L1 and the secondary conjugate streptavidin-saporin ...
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No products found
because this supplier's products are not listed.
Elena Arutyunova, et al.,
bioRxiv - Biophysics 2021
Quote:
... The fusion protein was subsequently digested with His-tagged SUMO protease (McLab, South San Francisco, CA) at 4 °C for 1–2 h to remove the SUMO tag and the resulting cleavage mixture was then passed through Ni-NTA resin column ...
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No products found
because this supplier's products are not listed.
Coralie Berthoux, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Recombinant human BDNF protein was purchased from Hello Bio. Salts for making ACSF and internal solutions were purchased from Sigma-Aldrich.
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No products found
because this supplier's products are not listed.
Menglin Shi, Lei Zhao, Yong Wang,
bioRxiv - Plant Biology 2021
Quote:
The recombinant HPRs was purified with HIS-Select nickel affinity gel filler (CoWin Biosciences, Beijing, China). Briefly ...
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No products found
because this supplier's products are not listed.
Jiao Wang, et al.,
bioRxiv - Immunology 2020
Quote:
... Recombinant human interleukin-12 (IL-2) was gifted from Akron Biotech. Recombinant human interleukin-15 (IL-15 ...
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No products found
because this supplier's products are not listed.
Susanna Sauret-Güeto, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... For L1 reactions an LDV source plate (Labcyte) with all necessary L0 plasmids was needed ...
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No products found
because this supplier's products are not listed.
Peter Hanna, et al.,
bioRxiv - Neuroscience 2020
Quote:
... A quadripolar His catheter (Abbott) was placed via the right external jugular vein and advanced until a His signal was visualized while a quadripolar catheter was inserted via the right femoral vein and advanced to the right atrium ...
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No products found
because this supplier's products are not listed.
Marcin Poreba, et al.,
bioRxiv - Biochemistry 2019
Quote:
... In prior to kinetic studies all cathepsins were active site titrated using E64 inhibitor (Peptide Institute, Japan). Human recombinant pro-legumain (asparaginyl endopeptidase ...
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No products found
because this supplier's products are not listed.
Julia A Alvarez, et al.,
bioRxiv - Microbiology 2024
Quote:
... 20% heat inactivated (HI) fetal bovine serum (FBS) (Omega Scientific), 1% penicillin-streptomycin (Life Technologies ...
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No products found
because this supplier's products are not listed.
Jan-Renier A.J. Moonen, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Flag-tagged KLF4 (#VH829440, Vigene Biosciences) or GFP control (AVP004, GenTarget Inc) for 12 h after which cells were allowed to recover for 90 h before being harvested for subsequent experiments.
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No products found
because this supplier's products are not listed.
Hiroshi Ichise, et al.,
bioRxiv - Immunology 2021
Quote:
... Recombinant mouse thrombin was obtained from antibodies-online GmbH (Aachen ...
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No products found
because this supplier's products are not listed.
William S. Lawrence, et al.,
bioRxiv - Microbiology 2023
Quote:
... Biotinylated recombinant PA83 (List Biological Labs, Campbell, CA) was bound to streptavidin-coated plates (MesoScale Discovery ...
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No products found
because this supplier's products are not listed.
Xiaoning Yang, et al.,
bioRxiv - Immunology 2023
Quote:
... C1q recombinant protein (A400) was purchased from Quidel and its secondary anti C1q/HRP (ab46191 ...
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No products found
because this supplier's products are not listed.
Yaiza M. Castillo, et al.,
bioRxiv - Microbiology 2019
Quote:
... NCBI accession number txid70448) was grown in 60 mL of L1 medium (Guillard and Hargraves, 1993) in aerated flasks (Sarstedt), and incubated at 21.5°C (±0.5°C ...
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No products found
because this supplier's products are not listed.
Alexander Solovyov, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... we require that the call is present in all pairwise comparisons where it should be present with the correct filtering status (e. g., if a given L1 element is present only in the maternal genome in PacBio calls ...
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No products found
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Matheus F. Sathler, et al.,
bioRxiv - Neuroscience 2022
Quote:
... NIH: HIV-1 IIIB gp120 Recombinant Protein from ImmunoDX, LLC ...
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No products found
because this supplier's products are not listed.
Ben S. Ou, et al.,
bioRxiv - Bioengineering 2023
Quote:
... HIS Lite Cy3 Bis NTA-Ni Complex was purchased from AAT Bioquest. Unless otherwise stated ...
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No products found
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Advika Kamatar, et al.,
bioRxiv - Biophysics 2023
Quote:
... His-Clathrin was labeled with Atto594 NHS-ester (ATTO-TEC, Sigma-Aldrich) according to a previously published protocol (41 ...
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No products found
because this supplier's products are not listed.
Kenji Shimada, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... then 0.002 U of recombinant hOGG1 (Trevigen, 4130-100-E) was added to one sample and further incubated for 30 min at 37°C ...
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No products found
because this supplier's products are not listed.
Arun Upadhyay, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 10 nM recombinant MT3 protein (Boster Bio Cat no. PROTP25713) was added at a in additional wells ...
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No products found
because this supplier's products are not listed.
Iromi Wanigasuriya, et al.,
bioRxiv - Genomics 2022
Quote:
... The 3’ end of each oligonucleotide probe was fluorescently tagged using Quasar dyes (Biosearch technologies). Bl6-specific oligos were labelled with Quasar 570 and Cast-specific oligos labelled with Quasar 670 ...
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No products found
because this supplier's products are not listed.
Marisa Oliveira, et al.,
bioRxiv - Immunology 2020
Quote:
... and chicken interferon alpha (Yeast-derived Recombinant Protein, Kingfisher Biotech, Inc) were diluted in nuclease-free water (Ambion ...
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No products found
because this supplier's products are not listed.
Attila Ozsvár, et al.,
bioRxiv - Neuroscience 2020
Quote:
... were pressure-injected with a glass pipette (1-2 MΩ) in L1 cortical region under the visual guide of Zeiss Axio Examiner LSM7 (Carl Zeiss AG, Oberkochen, Germany) two-photon microscope using 40x water immersion objective (W-Plan ...
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No products found
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Wiktoria Ogrodzińska, et al.,
bioRxiv - Biochemistry 2024
Quote:
... The recombinant plasmids were isolated using plasmid purification kit (Bio Basic Canada) and the inserts were verified by sequencing (Genomed ...
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No products found
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Nicolas Lamassiaude, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... Recombinant constructs were purified using EZNA Plasmid DNA Mini kit (Omega Bio-Tek) and sequence-checked (Eurofins Genomics) ...
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No products found
because this supplier's products are not listed.
Chungwon Park, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Neurons were pre-incubated with 3 nM of CypHer5E-tagged anti-VGAT antibodies (131 103CpH, Synaptic Systems, Germany) in the culture medium and incubated in the 5% CO2 incubator for 2 h at 37°C to specifically label inhibitory synaptic vesicles in presynaptic terminals ...
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No products found
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Daniel Schindler, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... The DNA Hi-C libraries were sheared into 300 bp using a Covaris S220 apparatus (Covaris) and the biotin-labeled fragments were selectively captured by Dynabeads Myone Streptavidin C1 ...
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CDH18 is expressed specifically in the central nervous system and is putatively involved in...
Cat# 5090-0.1MG,
0.1 mg, USD $235.0
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Haiqing Bai, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Human collagen-I (Advanced Biomatrix, #5007) was prepared according to manufacturer’s instruction ...
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No products found
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Antonin Tutter, et al.,
bioRxiv - Biochemistry 2024
Quote:
... the indicated concentrations of purified 6His-tagged BRD4 were spotted onto blank nitrocellulose-coated glass slides (Grace Bio-Labs PATH protein microarray slides ...
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No products found
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Aditi Deshpande, et al.,
bioRxiv - Microbiology 2022
Quote:
... library preparation and Hi-Seq 2 x150 paired-end sequencing were performed by GENEWIZ (New Jersey, USA).
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No products found
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Aruna Pal, Abantika Pal, Pradyumna Baviskar,
bioRxiv - Genomics 2020
Quote:
... Gene fragment insert in the recombinant plasmid was sequenced by an automated sequencer (ABI prism) using the dideoxy chain termination method with T7 and SP6 primers (Chromous Biotech ...
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No products found
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Nazila V. Jafari, Jennifer L. Rohn,
bioRxiv - Microbiology 2022
Quote:
HBLAK human bladder progenitor cells (CELLnTEC, Switzerland) were grown according to the CELLnTEC protocol ...
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No products found
because this supplier's products are not listed.
Alina Remeeva, et al.,
bioRxiv - Biophysics 2021
Quote:
... with the mutation C85A and C-terminal 6×His tag) was obtained as a synthetic gene from Evrogen (Russia) and introduced into the pET11 expression vector (Novagen ...
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No products found
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Yuichi Mitsui, et al.,
bioRxiv - Immunology 2022
Quote:
Human PBMCs were purchased from Precision for Medicine, Inc ...
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No products found
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Mutsumi Kobayashi, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Human iPSCs were dissociated using Accutase (Innovative Cell Technologies, AT104) and suspended in mTeSR plus (Stemcell Technologies ...
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No products found
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JM Robinson, et al.,
bioRxiv - Immunology 2019
Quote:
... I-FABP (Human I-FABP ELISA Kit, Hycult biotech, Cat# HK406), and LBP (Human Lipopolysaccharide Binding Protein ELISA Kit ...
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No products found
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Ida M. Modvig, et al.,
bioRxiv - Physiology 2019
Quote:
... Radioactive labeled rat and human secretin was obtained from Phoenix Pharmaceuticals, Inc (cat no ...
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No products found
Daniel J. Steinberg, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 2.5µg/ml human recombinant Insulin (Biological Industries; 41-975-100) and 3µM CHIR-99021 (Axon Medchem ; 1386) sterilized through 0.22μm filter ...
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No products found
because this supplier's products are not listed.
Matthew J. Stevenson, et al.,
bioRxiv - Cancer Biology 2022
Quote:
DNA sequences flanked by XhoI and NdeI restriction sites and encoding for N-terminal GST-tagged-SIX1 or N-terminal GST-tagged-SIX1-Q177R homeodomains were synthesized by Integrative DNA Technologies (IDT) as gBlocks (Supplemental Table 2) ...
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No products found
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Lucia Gonzalo, et al.,
bioRxiv - Plant Biology 2021
Quote:
... 4°C and the supernatants were incubated overnight with 1/100 RNAPII or HIS antibodies (RNAP II AS11 1804, HIS AS20 4441, Agrisera) and 30 μl SureBeads (BioRad) ...
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No products found
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Tiesuo Zhao, et al.,
bioRxiv - Immunology 2024
Quote:
... the membranes were incubated with the following primary antibodies overnight at 4 ℃: PD-L1 (1:1000, Bioworld), p-Stat3 (1:1000 ...
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No products found
because this supplier's products are not listed.
Bhaskar Saha, et al.,
bioRxiv - Cell Biology 2023
Quote:
... All GST-tagged proteins were expressed in Escherichia coli SoluBL21 (Genlantis). GST fusion proteins were purified on glutathione-Sepharose 4 Fast Flow beads (GE Healthcare 17-5132-01) ...
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No products found
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Joe Chin-Hun Kuo, et al.,
bioRxiv - Bioengineering 2024
Quote:
... elegans eggs were hatched into L1 larvae for 24 h without food in M9 buffer containing 1 mM tetra-acylated azidoacetylgalactosamine (GalNAz, Click Chemistry Tools) or M9 buffer containing equal volume of the vehicle (DMSO) ...
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No products found
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Surbhi Verma, et al.,
bioRxiv - Microbiology 2023
Quote:
... Human MDMs (hMDMs) were isolated from human PBMCs and separated from healthy human donors’ blood using PolymorphPrep (PROGEN,1895) layering and centrifugation ...
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No products found
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Rahul Kumar, et al.,
bioRxiv - Cell Biology 2023
Quote:
GST-MICAL-L1-CC protein was expressed in Escherichia coli BL21 (500 μM isopropyl β-d-1-thiogalactopyranoside; Wisent Bioproducts; at room temperature for 16 h) and purified using standard procedure in Tris buffer (20 mM Tris ...
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No products found
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Tracy J. Berg, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Primary human astrocytes (3H Biomedical) were cultured in Astrocyte Medium (3H Biomedical ...
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No products found
because this supplier's products are not listed.
Tomofumi Nakamura, et al.,
bioRxiv - Microbiology 2019
Quote:
Recombinant INHis proteins were examined with a TSKgel-SWXLG3000 column (TOSOH cor., Japan) at 1.0 ml/min in running buffer (20 mM HEPES pH 7.4 ...
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No products found
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Colin Lock, Melissa M. Gabriel, Bastian Bentlage,
bioRxiv - Molecular Biology 2023
Quote:
DNA was extracted from each tagged colony using the GenCatch genomic DNA extraction kit (Epoch Life Science, Sugar Land, TX) following the manufacturer’s protocol for tissue samples ...
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No products found
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Ava P. Soleimany, et al.,
bioRxiv - Bioengineering 2020
Quote:
Fresh frozen human PCa TMAs (BioChain Institute, Inc.; T6235201) were air dried and fixed in ice-cold acetone for 10 minutes ...
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No products found
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Tommy Tong, et al.,
bioRxiv - Immunology 2023
Quote:
... followed by anti-human IgG AP conjugate (Accurate Chemicals) and were developed using SigmaFast BCIP/NBT ...