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No products found
because this supplier's products are not listed.
Jessica L. Kelliher, et al.,
bioRxiv - Microbiology 2023
Quote:
... Endpoint kinase assays were performed by mixing 3 μg purified PrkA1- 338 with the indicated combinations of ∼3x molar excess of substrate to kinase (6 μg of the generic kinase substrate myelin basic protein [MBP; Novatein Biosciences, Woburn ...
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No products found
because this supplier's products are not listed.
Anna Vilalta, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Proteins in storage buffer were concentrated to between 5-10 mg/ml for use and tested for endotoxin with the EndoZyme recombinant Factor C assay (Hyglos GmbH). Endotoxin levels in all assays were maintained at < 0.1 EU/ml.
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No products found
because this supplier's products are not listed.
Jesica Romina Canizo, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... The Large Tumor Suppressor Kinase (LATS) inhibitor (TRULI, Enamine; Z730688380)3,60 and the PKC inhibitor (Gö6983 ...
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Cat# AB-T030,
50 microliters,USD $330.0
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Stephen P. Persaud, et al.,
bioRxiv - Immunology 2020
Quote:
... non-interaction of these two components was ensured by using non-biotinylated antibodies and sAV-SAP whose biotin-binding sites were occupied by an irrelevant biotinylated 11-mer peptide (BLANK Streptavidin-SAP, Advanced Targeting Systems). For experiments in which free antibody or sAV-SAP were administered alone ...
-
No products found
because this supplier's products are not listed.
Katherine P. Mueller, et al.,
bioRxiv - Bioengineering 2021
Quote:
... Recombinant sNLS-SpCas9-sNLS Cas9 (Aldevron, 10 mg/ml, total 0.8 µl) was added to the complexed gRNA at a 1:1 molar ratio and incubated for 15 minutes at 37°C to form an RNP ...
-
No products found
because this supplier's products are not listed.
Sameh S. M. Soliman, et al.,
bioRxiv - Microbiology 2021
Quote:
Rabbit and mouse monoclonal antibodies against recombinant mucoricin coupled to KLH were raised by ProMab Biotechnologies Inc.11 ...
-
No products found
because this supplier's products are not listed.
Rueyhung R. Weng, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... 5% human platelet lysate (PLS1, Compass Biomedical), 70 ng/mL IL-15 (AF-200-15 ...
-
No products found
because this supplier's products are not listed.
Tetsuro Yamamoto, et al.,
bioRxiv - Immunology 2023
Quote:
... HRP-human IgG antibody (EY Laboratories, USA), and BT IgE antibody (Bio-Rad Laboratories ...
-
No products found
because this supplier's products are not listed.
Menglin Shi, Lei Zhao, Yong Wang,
bioRxiv - Plant Biology 2021
Quote:
The recombinant HPRs was purified with HIS-Select nickel affinity gel filler (CoWin Biosciences, Beijing, China). Briefly ...
-
No products found
because this supplier's products are not listed.
Young Sun Hwang, M. Andrés Blanco, Kotaro Sasaki,
bioRxiv - Developmental Biology 2022
Quote:
... hiPSCs were cultured on plates coated with recombinant laminin-511 E8 (iMatrix-511 Silk, Nacalai USA) and maintained under feeder-free conditions in StemFit® Basic04 medium (Ajinomoto ...
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No products found
because this supplier's products are not listed.
Patricia Aguilar-Calvo, et al.,
bioRxiv - Pathology 2023
Quote:
... full length recombinant mouse PrP (23-230) generated in E.coli was first conjugated to deferoxamine-maleimide (Macrocyclics) to produce deferoxamine-conjugated PrPC (DFO-PrPC) ...
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No products found
Glory Nasseri, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Human PRG-1 obtained from DNASU Plasmid Repository (The Biodesign Institute/Arizona State University ...
-
No products found
because this supplier's products are not listed.
James L. J. Coleman, et al.,
bioRxiv - Biochemistry 2020
Quote:
... A previously reported (16,54) human GPR37L1 was purchased from Multispan; however ...
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No products found
because this supplier's products are not listed.
Padmapriya Sekar, et al.,
bioRxiv - Immunology 2023
Quote:
... 330 μg/mL iron-saturated human holo-transferrin (BBI solutions), 10 μg/mL recombinant human insulin (Sigma-Aldrich) ...
-
No products found
because this supplier's products are not listed.
Xiaoke Chi, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Membranes were pelleted by centrifugation at 13000g for 10 min and cytochrome c release was analyzed by immunoblotting using a sheep anti-cytochrome c antibody (Capralogics). Mitochondria from embryonic mouse brains for BH3 profiling experiments were prepared from ~20 mouse embryos ...
-
No products found
because this supplier's products are not listed.
Jasmine Alexander-Floyd, et al.,
bioRxiv - Immunology 2021
Quote:
... supernatants and recombinant cytokine standards were applied to anti-IL-1β antibody-coated (eBioscience) Immulon ELISA plates (ImmunoChemistry Technologies). IL-1β was detected using biotinylated anti IL-1β (eBioscience ...
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No products found
because this supplier's products are not listed.
Dennis Alejandro Escolástico-Ortiz, et al.,
bioRxiv - Microbiology 2023
Quote:
... followed by 1 h at 45°C and 2 h at 65°C in a heating block digestion system (DigiPREP, SCP Sciences). After digestion ...
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No products found
because this supplier's products are not listed.
Livia Mazzini, et al.,
bioRxiv - Immunology 2020
Quote:
ELISA plates were coated with 1µg/mL of purified recombinant Spike S1 Protein (aa 18-676) (eEnzyme, Gaithersburg, MD, USA) or with 1µg/mL Spike-RBD (Arg319-Phe541 ...
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No products found
because this supplier's products are not listed.
Masaya Matsubayashi, et al.,
bioRxiv - Biochemistry 2019
Quote:
The human hepatocellular carcinoma cell HepG2 was purchased from Cellular Engineering Technologies ...
-
No products found
because this supplier's products are not listed.
Marcos A.S. Fonseca, et al.,
bioRxiv - Genomics 2021
Quote:
... 60°C/4min on an AC4 thermal cycler (FroggaBio). 5-fold diluted pre-amplified DNA was used in multiplex ddPCR and subsequently individual-variant ddPCR for validation (if positive) ...
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No products found
because this supplier's products are not listed.
Kyle E. Landgraf, et al.,
bioRxiv - Cancer Biology 2019
Quote:
Human PBMC stimulation and immune-phenotyping studies were performed by iQ Biosciences. Briefly normal PBMCs from three donors were seeded in 96-well plates at 1×105 cells/well and exposed to a 10-fold dilution series of either U2S3-hFc-mutIL2 or U2S3-hFc-wtIL2 (wild-type IL2 ...
-
No products found
because this supplier's products are not listed.
Aaron M. Rosado, et al.,
bioRxiv - Biophysics 2022
Quote:
... freshly isolated human RBCs were biotinylated with biotin-PEG3500-NHS (Jenkem Technology) and then incubated with nystatin in N2 buffer (265.2 mM KCl ...
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No products found
because this supplier's products are not listed.
Irene Pereira de Sousa, et al.,
bioRxiv - Bioengineering 2019
Quote:
... 50 mg of dried MSPs were degassed for 1 h at 100 °C with nitrogen and then analyzed by nitrogen sorption at −196 °C (TriStar 3000, Micromeritics, Norcross, GA, USA). The morphology of MSPs was assessed by SEM (Quanta 200F ...
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No products found
because this supplier's products are not listed.
Erdem D. Tabdanov, et al.,
bioRxiv - Cancer Biology 2020
Quote:
Human CD4+ T cells were plated on a glass-bottom dish (Willco Wells) pre-coated with either ICAM-1 (Life Technologies ...
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No products found
because this supplier's products are not listed.
Guillaume N. Fiches, et al.,
bioRxiv - Microbiology 2021
Quote:
... supplemented with 10% heat-inactivated human serum (Advanced Biotechnologies Inc, Cat#P2-203), 100 U/mL penicillin ...
-
No products found
because this supplier's products are not listed.
Madelon M.E. de Jong, et al.,
bioRxiv - Immunology 2023
Quote:
... slides were incubated with anti-human CD15 (1:10, C3D-1, Diagnostic BioSystems) for 1 hour at 37°C ...
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No products found
because this supplier's products are not listed.
Eli Fritz McDonald, et al.,
bioRxiv - Cell Biology 2021
Quote:
... purified from B lymphocyte hybridoma cells using a Recombinant Protein G - Sepharose 4B Affinity Column on an ÄKTA start protein purification system (GE Life Science Product # 29022094). Antibodies were used in immunoblotting buffer (5% bovine serum albumin (BSA ...
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No products found
because this supplier's products are not listed.
Alberto Domingo López-Muñoz, et al.,
bioRxiv - Microbiology 2023
Quote:
... alone or in combination with purified recombinant proteins were placed in the lower chamber of a 96-well ChemoTx System plate (Neuro Probe # 101-5) in RPMI 1640 1 % FBS ...
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No products found
because this supplier's products are not listed.
Ekaterina Kozaeva, et al.,
bioRxiv - Synthetic Biology 2024
Quote:
... rapidly frozen by plunging into slushed liquid nitrogen (N2) at −210°C and transferred to the preparation chamber stage at −180°C (PP2000 CryoTransferTM System; Quorum Technologies, Laughton, East Sussex, United Kingdom). Frozen samples were cleaved with a cold knife (facilitating an exposed surface in the fractured sample) ...
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No products found
because this supplier's products are not listed.
Matthew J. Shannon, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... The assay was validated using a standard curve calibrated against human methylation controls (EpigenDx, USA Cat ...
-
No products found
because this supplier's products are not listed.
Duilio M. Potenza, et al.,
bioRxiv - Physiology 2024
Quote:
... and human cardiac fibroblasts was extracted with Trizol Reagent (TR-118, Molecular Research Center) following the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Christian S. Stevens, et al.,
bioRxiv - Microbiology 2022
Quote:
... and fluorescent probe (5’-6FAM-ACAGACGTTGTATA+C+CAT+G-TMR) (TIB MOLBIOL) RT-qPCR was performed using the following cycle ...
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No products found
because this supplier's products are not listed.
Gabriela Zurawska, et al.,
bioRxiv - Cell Biology 2023
Quote:
... mice received i.v.solution of liposomes containing clodronic acid (LIPOSOMA, #C-SUV-005) (5 ml/kg ...
-
No products found
because this supplier's products are not listed.
Jonah C. Rosch, et al.,
bioRxiv - Bioengineering 2021
Quote:
... Fluorescently labeled human serum albumin (HS1-S5-1) was purchased from NANOCS (New York, NY). The starting DNA library ...
-
No products found
because this supplier's products are not listed.
Andrew I. Wong, et al.,
bioRxiv - Microbiology 2022
Quote:
... samples were separated on a Cogent Diamond Hydride Type C column (Microsolv Technologies). The mobile phase consisted of solvent A (ddH2O with 0.2% formic acid ...
-
No products found
because this supplier's products are not listed.
Niamh E. Harrington, et al.,
bioRxiv - Microbiology 2019
Quote:
... Tissue was incubated at 37 °C with a Breathe-Easier® membrane (Diversified Biotech) for the desired length of time.
-
No products found
because this supplier's products are not listed.
Benjamin D. Hobson, et al.,
bioRxiv - Neuroscience 2022
Quote:
... A small hole was drilled into the skull and 400 nL of 0.9% saline or 0.9% saline + 100 ng/μL recombinant mouse IFN-γ (40 ng IFN-γ total) was injected through a pulled glass pipet using a Nanoject 2000 (Drummond Scientific; 8 pulses of 50 nL). At 5 minutes after injection ...
-
No products found
because this supplier's products are not listed.
Josiane Reist, et al.,
bioRxiv - Microbiology 2019
Quote:
... influenzae isolates were frozen at −70 °C in cryogenic MicrobankTM vials (Pro-Lab Diagnostics, Birkenhead, UK). Prior to testing ...
-
No products found
because this supplier's products are not listed.
Hitika Gulabani, et al.,
bioRxiv - Plant Biology 2021
Quote:
... rinsed and incubated overnight at 4°C with indicated primary antibodies [anti-SNC1 (Abiocode; R3588-1), anti-PR1 ...
-
No products found
because this supplier's products are not listed.
Ryan S. Nett, et al.,
bioRxiv - Biochemistry 2022
Quote:
... huperzine C (16; two independent sources: Shanghai Tauto Biotech Co., Ltd and Toronto Research Chemicals Inc.), huperzine A (17 ...
-
No products found
because this supplier's products are not listed.
Katy Pilarzyk, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Primary antibody was applied overnight at 4°C for PDE11A (Fabgennix PD11-101 at 1:500). The following day ...
-
No products found
because this supplier's products are not listed.
Marco Losa, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... were dispensed at various volumes into human ASC-coated 1,536-well plates using contactless dispensing with an ECHO 555 Acoustic Dispenser (Labcyte). Thereby ...
-
No products found
because this supplier's products are not listed.
MU Wagenhäuser, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... two-color analysis of murine or human platelet activation was performed using fluorophore-labeled antibodies for P-selectin expression (Emfret Analytics, Wug.E9-FITC ...
-
No products found
because this supplier's products are not listed.
Ram Sagar, et al.,
bioRxiv - Genetics 2023
Quote:
To acquire a high yield of functional neurons we transduced the generated human iPSCs with Ngn2 and rTTA expressing lentivirus (lentivirus was purchased by Cellomics Technology, Maryland USA). We followed an established protocol for generation of induced neuronal cells in 21 days 17 ...
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No products found
because this supplier's products are not listed.
Xindi Li, et al.,
bioRxiv - Neuroscience 2024
Quote:
... human-derived microglia were subjected to a seven-day chronic intermittent exposure (CIE) paradigm using 200 Proof ethanol from Decon Laboratories (catalog# 2716) at concentrations of 0 mM (control) ...
-
No products found
because this supplier's products are not listed.
Jimi L. Rosenkrantz, et al.,
bioRxiv - Developmental Biology 2021
Quote:
BeWo cells were cultured at 37°C 5% CO2 in Ham’s F-12 (Kaighn’s Modification) media (Caisson Labs, HFL06-500ML) supplemented with 10% FBS (Sigma ...
-
No products found
because this supplier's products are not listed.
Huibao Feng, et al.,
bioRxiv - Genetics 2022
Quote:
... for 1 h at 37°C for recovery and then plated onto 37 Φ150 LB agar plates containing kanamycin with an EasySpiral Pro (Interscience). Generally ...
-
No products found
because this supplier's products are not listed.
Soo-Yeon Kang, et al.,
bioRxiv - Bioengineering 2023
Quote:
... HLOs were cleared by incubating in 30 μL of 1x pre-warmed (at 37°C) RapiClear 1.52 (Sunjin Lab, Taiwan) or Visikol® Histo-M™ (SKU ...
-
No products found
because this supplier's products are not listed.
Liangliang Hao, et al.,
bioRxiv - Bioengineering 2020
Quote:
... The reduced C-terminal cysteine (1 eq.) was reacted with sulfo DBCO-maleimide crosslinker (4 eq.) (Click Chemistry Tools, AZ, USA) in PBS (pH 6.5 ...
-
No products found
because this supplier's products are not listed.
An Phu Tran Nguyen, et al.,
bioRxiv - Neuroscience 2019
Quote:
... sections were pre-incubated in 4% PFA (in 0.1 M PB) for ≥2 days at 4°C before processing with the FD NeuroSilver™ kit II (FD Neurotechnologies, Inc.) according to the manufacturer’s protocol ...