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... Immunocomplexes were visualized using the ECL substrates kits from Cyanagen and imaged on ALLIANCE MINI HD9 system (UVITEC Ltd, UK). Quantification of bands was performed using the Uvitec Nine Alliance Software ...
Laura Ohl, et al.,bioRxiv - Molecular Biology 2023
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... Tissue was mixed with 1 mL 40:40:20 methanol:acetonitrile:water (extraction solvent) and homogenized with a benchtop lyser (SciLogex OS20-S) at 1600 mhZ for approximately 15 sec ...
Kevin M. Tharp, et al.,bioRxiv - Cell Biology 2020
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... 1 μL of sample was separated over a 25 cm column packed with 1.9 μm Reprosil C18 particles (Dr. Maisch HPLC GmbH) by a nanoElute HPLC (Bruker) ...
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... was homogenised for 30 seconds in 90 mL of sterile 1× phosphate buffered saline (PBS) using a BagMixer® 400 P laboratory blender (Interscience, Edwards Group). This was repeated two more times to obtain a total of three replicates per pool sample ...
Kathleen L. Arnolds, et al.,bioRxiv - Microbiology 2023
Quote:
Mesocosm experiments were conducted in 3D-printed pots (S. Fig. 1) that have 18 sampling ports which are sealed with neoprene stoppers (Eisco Labs, Victor, NY). A perforated base allows for root development ...