-
No products found
because this supplier's products are not listed.
Lorna Ewart, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... Cryopreserved human Kupffer cells (Samsara Sciences) and human Stellate cells (IXCells) were thawed according to their respective vendor/Emulate protocols on the day of seeding ...
-
No products found
because this supplier's products are not listed.
Camden R. Bair, et al.,
bioRxiv - Microbiology 2021
Quote:
... supplemented with 8% heat-inactivated newborn calf serum (HI-NBCS, Rocky Mountain Biologicals), 1.5 g/ml Na2CO3 (Amresco) ...
-
No products found
because this supplier's products are not listed.
Neil J Rzechorzek, et al.,
bioRxiv - Biochemistry 2020
Quote:
... HEK293-F cells were grown in 400 mL batches in 2 L non-baffled Erlenmeyer flasks (TriForest Enterprises Inc, #FPC2000S) to a cell density of ∼1×106 cells/mL.
-
No products found
because this supplier's products are not listed.
Marta Fontcuberta-PiSunyer, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... Human insulin was measured using a human Insulin ELISA kit (Crystal Chem, Zaandam, Netherlands) and C-peptide using a human C-peptide ELISA kit (Mercodia ...
-
No products found
because this supplier's products are not listed.
Anne Rehkamp, et al.,
bioRxiv - Biochemistry 2020
Quote:
LC separation of peptides originating from HEK293 cell membranes was performed via selfpacked C18 columns (PicoFrits, 75 μm x 50 cm, 15 μm tip, New Objective, self-packed with ReproSil-Pur 120 C18-AQ ...
-
No products found
because this supplier's products are not listed.
Ronan Shaughnessy, et al.,
bioRxiv - Cell Biology 2023
Quote:
... lentivi-ral particles were produced in the HEK293 FT packaging cells transfected with MiniBAR constructs and the Excelenti LTX Lentivirus Packaging mix (Oxford Genetics) using lipo-fectamine following manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Andrew R. McEwan, et al.,
bioRxiv - Genetics 2021
Quote:
... from human DNA (Cambio, UK) using the following primers ...
-
No products found
because this supplier's products are not listed.
Emilie L. Cerezo, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Human EGF (Euromedex, Cat# HC88823), LJH685 (Selleck Chemicals ...
-
No products found
because this supplier's products are not listed.
Elena Arutyunova, et al.,
bioRxiv - Biophysics 2021
Quote:
... The fusion protein was subsequently digested with His-tagged SUMO protease (McLab, South San Francisco, CA) at 4 °C for 1–2 h to remove the SUMO tag and the resulting cleavage mixture was then passed through Ni-NTA resin column ...
-
No products found
because this supplier's products are not listed.
Lin Zhuang, et al.,
bioRxiv - Molecular Biology 2023
Quote:
Human colon normal epithelial cells (FHC) and CRC cell lines (HT29 and LoVo ...
-
No products found
because this supplier's products are not listed.
Cameron O. Zadeh, et al.,
bioRxiv - Biochemistry 2021
Quote:
Horizontal electrophoresis was carried out using the Flatbed Professional (Gel Company Store, FC-EDCProf-2836). The apparatus was maintained at 10°C during electrophoresis ...
-
No products found
because this supplier's products are not listed.
Jonathan M Downie, et al.,
bioRxiv - Genetics 2019
Quote:
... HEK293T cells were incubated in high glucose Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% heat inactivated fetal bovine serum (HI FBS, Genesee Scientific), and 1% pen/strep (P/S ...
-
No products found
because this supplier's products are not listed.
Chanchal Thomas Mannully, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... 1 μg/mL of human TGFβ1 (Biogems, Peprotech).
-
No products found
because this supplier's products are not listed.
Darko Bosnakovski, et al.,
bioRxiv - Molecular Biology 2023
Quote:
Control LHCN-M2 immortalized human myoblasts 35 and FSHD (M008) immortalized human myoblasts were cultured in F10 medium (HyClone) with 20% FBS (Peak Serum), 2-mercaptoethanol 1x (Gibco) ...
-
No products found
because this supplier's products are not listed.
Swathi Jayaram, Merrit Romeike, Christa Buecker,
bioRxiv - Developmental Biology 2023
Quote:
... Cell pellets were resuspended in PBS to remove residual FCS and counted with a CASY cell counter (Biovendis). Labeling of cells with MULTI-Seq barcodes was performed as described34 ...
-
No products found
because this supplier's products are not listed.
Aman Y. Husbands, et al.,
bioRxiv - Plant Biology 2022
Quote:
... Membranes were washed three times with PBS-T and incubated with 1:2000 anti-His-tag antibody (Abiocode M0335-1) for 1h with gentle shaking at RT ...
-
No products found
because this supplier's products are not listed.
Yan Wang, Meiling Lian, Liping Song, Shengzhou Wu,
bioRxiv - Neuroscience 2020
Quote:
... fractions using commercially available kits (Cat#CSB-E08299h for human Aβ40; Cat#CSB-E10684h for human Aβ42, CUSABIO TECHNOLOGY LLC, Wuhan, China) per the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Carlos J Nogueras-Ortiz, et al.,
bioRxiv - Neuroscience 2020
Quote:
... or anti-human tetraspanin CD81 (Ancell Corporation, Bayport, MN) biotinylated antibodies ...
-
No products found
because this supplier's products are not listed.
Vaibhav Sidarala, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Anti-human TFAM (1:1000, PhosphoSolutions, Catalog# 1999-hTFAM), Anti-mouse TFAM (1:1000 ...
-
No products found
because this supplier's products are not listed.
Nirupa Nagaratnam, et al.,
bioRxiv - Biochemistry 2022
Quote:
... The supernatant containing detergent solubilized His-tagged FopA was filtered through a 0.22 μm cellulose acetate membrane filter (Foxx Life Sciences) and loaded at 1 mL/min onto a Ni-NTA column (HisTrap HP ...
-
No products found
because this supplier's products are not listed.
Yunlong Li, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... the full-length codon optimized gene with N-terminal 6x-His-sequnce and NdeI-XhoI sites was synthesized (IDT DNA Inc.) (Table S2) ...
-
No products found
because this supplier's products are not listed.
Shahrnaz Kemal, et al.,
bioRxiv - Neuroscience 2023
Quote:
... All vectors contain an N-terminal 6x-His-tag, C terminal Strep-Tag II tag, with or without a N or C terminal fluorophore (EGFP, mNeonGreen (Allele Biotech), or mRuby2).
-
No products found
Glory Nasseri, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Human PRG-1 obtained from DNASU Plasmid Repository (The Biodesign Institute/Arizona State University ...
-
No products found
because this supplier's products are not listed.
Shaun M. Christie, et al.,
bioRxiv - Biophysics 2021
Quote:
... Recombinant human Semaphorin 3A (CX65, Bon Opus Biosciences, Milburn, NJ) contains residues 21-771 and is >95% pure ...
-
No products found
because this supplier's products are not listed.
James L. J. Coleman, et al.,
bioRxiv - Biochemistry 2020
Quote:
... A previously reported (16,54) human GPR37L1 was purchased from Multispan; however ...
-
No products found
because this supplier's products are not listed.
Masaya Matsubayashi, et al.,
bioRxiv - Biochemistry 2019
Quote:
The human hepatocellular carcinoma cell HepG2 was purchased from Cellular Engineering Technologies ...
-
No products found
because this supplier's products are not listed.
Natsuki Shichinohe, et al.,
bioRxiv - Biochemistry 2022
Quote:
Human bone ALP was purchased from CALZYME (San Luis Obispo, CA). Human CD73 His-tag was purchased from BPS Bioscience (San Diego ...
-
No products found
because this supplier's products are not listed.
Amy L. Kimble, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Frozen mouse and human brain tissues were mechanically homogenized (Next Advance Bullet Blender BB724M with 3.2 mm stainless steel beads using setting 4 for 4min at 4C) ...
-
No products found
because this supplier's products are not listed.
Jiao Wang, et al.,
bioRxiv - Immunology 2020
Quote:
... Recombinant human interleukin-12 (IL-2) was gifted from Akron Biotech. Recombinant human interleukin-15 (IL-15 ...
-
No products found
because this supplier's products are not listed.
Anna Ilaria Przytula-Mally, et al.,
bioRxiv - Biophysics 2023
Quote:
... for the human CPEB3 complex and PEG/Ion screen (Hampton Research) for the chimpanzee complex ...
-
No products found
because this supplier's products are not listed.
Satoshi Watanabe, et al.,
bioRxiv - Biochemistry 2023
Quote:
... The established stable cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Nacalai Tesque) supplemented with 4∼5% fetal calf serum (FCS; Thermo Fisher Scientific, or Nichirei Biosciences Inc) and 1% penicillin-streptomycin mixed solution (Nacalai Tesque ...
-
No products found
because this supplier's products are not listed.
Camilla Kjeldgaard Larsen, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... The cleaved proteins were incubated with Ni-NTA beads for revers his-purification and cleaved proteins were collected in the flow through and visualized on SDS-PAGE (Mini Protean Tris-Tricine Precast gels, BioRad).
-
No products found
because this supplier's products are not listed.
Kyle E. Landgraf, et al.,
bioRxiv - Cancer Biology 2019
Quote:
Human PBMC stimulation and immune-phenotyping studies were performed by iQ Biosciences. Briefly normal PBMCs from three donors were seeded in 96-well plates at 1×105 cells/well and exposed to a 10-fold dilution series of either U2S3-hFc-mutIL2 or U2S3-hFc-wtIL2 (wild-type IL2 ...
-
No products found
because this supplier's products are not listed.
Hongbing Zhang, et al.,
bioRxiv - Bioengineering 2020
Quote:
The E-ALPHA® human scFv antibody phage display libraries (Eureka Therapeutics) were used for the selection of human antibody constructs specific to SARS-CoV-2 spike protein ...
-
No products found
because this supplier's products are not listed.
Aaron M. Rosado, et al.,
bioRxiv - Biophysics 2022
Quote:
... freshly isolated human RBCs were biotinylated with biotin-PEG3500-NHS (Jenkem Technology) and then incubated with nystatin in N2 buffer (265.2 mM KCl ...
-
No products found
because this supplier's products are not listed.
S. Karkampouna, et al.,
bioRxiv - Cell Biology 2021
Quote:
Mouse and human liver tissues were homogenised with UltraTurrax homogenizer (T25 basic, IKA) and TRIpure reagent (Roche) ...
-
No products found
because this supplier's products are not listed.
Ghulam Destgeer, et al.,
bioRxiv - Bioengineering 2020
Quote:
... particles were incubated with varying concentrations of human recombinant NT-proBNP (HyTest, Finland) for 1 hr with subsequent washing ...
-
No products found
because this supplier's products are not listed.
Tomoyuki Ohno, et al.,
bioRxiv - Bioengineering 2022
Quote:
... 201B7 human iPS (RIKEN BRC, HPS0063) cells were maintained in StemFit AK02N medium (ReproCELL) in dishes coated with iMatrix-511 (Nippi) ...
-
No products found
because this supplier's products are not listed.
Vincent Mouilleau, et al.,
bioRxiv - Developmental Biology 2020
Quote:
Human SA001 embryonic stem cell (ESC) line (male, RRID: CVCL_B347) was obtained from Cellectis and used accordingly to the French current legislation (Agency of Biomedicine ...
-
No products found
because this supplier's products are not listed.
Matthew J. Shannon, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... The assay was validated using a standard curve calibrated against human methylation controls (EpigenDx, USA Cat ...
-
No products found
because this supplier's products are not listed.
Aereas Aung, et al.,
bioRxiv - Immunology 2021
Quote:
... slides were stained with human VRC01 mAb directly labeled with AF488 NHS ester (21820, Lumiprobe). For non-permeabilized sections ...
-
No products found
because this supplier's products are not listed.
Nandan Haloi, et al.,
bioRxiv - Biophysics 2020
Quote:
... followed by addition of HKII (human recombinant HKII, 60 kU/ml; Genway Biotech, Slan Diego, CA) into the cis chamber ...
-
No products found
because this supplier's products are not listed.
Stephen A. Schumacher, et al.,
bioRxiv - Physiology 2021
Quote:
Serum PTH concentrations were measured with a human-specific immunoradiometric assay (Scantibodies Laboratory, Santee, CA, USA) with a working range of 6.5-2328 pg/mL ...
-
No products found
because this supplier's products are not listed.
Adam J. Widman, et al.,
bioRxiv - Genomics 2022
Quote:
... cfDNA was then extracted from human blood plasma by using the Mag-Bind cfDNA Kit (Omega Bio-Tek). The protocol was optimized and modified to optimize yield28 ...
-
No products found
because this supplier's products are not listed.
Michal Schwartz, et al.,
bioRxiv - Microbiology 2022
Quote:
... using FAM labeled HCMV primer and probe: Human CMV HHV5 kit for qPCR using a glycoprotein B target (PrimerDesign); and HEX labeled RPP30 copy number assay for ddPCR (Bio-Rad) ...
-
No products found
because this supplier's products are not listed.
Marco Losa, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... were dispensed at various volumes into human ASC-coated 1,536-well plates using contactless dispensing with an ECHO 555 Acoustic Dispenser (Labcyte). Thereby ...
-
No products found
because this supplier's products are not listed.
Kyung-Jin Jang, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
Alpha Glutathione S-Transferase (α-GST): levels were quantified in human model effluent samples from the upper channel using an ELISA kit (DiaPharma). The assay was run following the vendor protocol using a standard curve ranging from 0-64 µg/L ...
-
No products found
because this supplier's products are not listed.
Yanhe Zhao, et al.,
bioRxiv - Biophysics 2021
Quote:
3 μl of the human sperm sample were applied to glow discharged copper R2/2 200-mesh holey carbon grid (Quantifoil Micro Tools) and plunge frozen in liquid ethane using an automatic plunge freezer (Vitrobot ...
-
No products found
because this supplier's products are not listed.
Brandon J. Gheller, et al.,
bioRxiv - Cell Biology 2019
Quote:
3 μL of human plasma were spiked with 3 μL amino acid isotope labelled internal standards (Cambridge Isotope Laboratories, #MSK-A2-1.2) and extracted with 250 μL –20 °C methanol for 10 min and centrifuged at 4°C for 10 min at 15 000 g ...
-
No products found
because this supplier's products are not listed.
Ram Sagar, et al.,
bioRxiv - Genetics 2023
Quote:
To acquire a high yield of functional neurons we transduced the generated human iPSCs with Ngn2 and rTTA expressing lentivirus (lentivirus was purchased by Cellomics Technology, Maryland USA). We followed an established protocol for generation of induced neuronal cells in 21 days 17 ...