Marissa Saenz, et al.,bioRxiv - Pharmacology and Toxicology 2022
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... Following precipitation of plasma proteins with acetonitrile containing 3.5 ng/mL buprenorphine-d4 (Cerilliant, Round Rock, TX, USA) as an internal standard ...
Paulus G.M. Jochems, et al.,bioRxiv - Bioengineering 2020
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... Protein content in the digests as assessed in triplicate on a Flash EA 1112 GC (Interscience BV, Breda, The Netherlands) according the Dumas method (45) ...
Ashok Narasimhan, et al.,bioRxiv - Cancer Biology 2023
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Myocardium proteins were extracted from cryo-fractured tissue using an automated tissue pulverizer (cyroPREP Tissue Disruption System, Covaris model CP02) prior to the addition of urea lysis buffer and sonication.
Minghui Di, et al.,bioRxiv - Molecular Biology 2023
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HeLa cells were transfected with DNA (1 ug) expressing mCherry-tagged proteins cultured on 29-mm glass-bottom dish (Cellvis); plasmids (1 ug ...
Sylvie Labrouche-Colomer, et al.,bioRxiv - Genetics 2020
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... The concentration of total and free PS antigen were determined by an enzyme-linked immunosorbent assay (Asserachrom total or free protein S; Diagnostica Stago). In normal conditions ...
Pragya D Yadav, et al.,bioRxiv - Microbiology 2021
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... Pseudovirus (an HIV-based luciferase expressing lentivirus pseudotyped with SARS-CoV-2 full length S protein) was obtained from Creative Biogene. One step luciferase assay kit from BPS Bioscience was used for detection ...
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... we determined the concentration of the positive acute phase protein Haptoglobin (Hp; Phase Haptoglobin Assay TP-801, Tridelta Development Limited, Maynooth, Ireland). All biomarkers related to nutrient utilization and inflammation were determined using a small-scale biochemistry analyzer (CataChemWell-T ...
Dmytro Morderer, et al.,bioRxiv - Neuroscience 2024
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... Immunostained sections were washed 3×5 minutes with Wash Buffer followed by HRP-mediated protein proximity labelling in Labelling Buffer (1xTBS, 5 uM biotin-ss-tyramide (Iris Biotech, LS-3570), 0.03% H2O2 ...
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... membranes were stripped at 4°C in agitation using Antibody stripping buffer 1X for 10 minutes (Gene Bio-Application). Protein bands were quantified using ImageJ software ...
Lauren G. Mascibroda, et al.,bioRxiv - Biochemistry 2020
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... pTEF or pTEFdual plasmids were included to express proteins in trans and were transformed with pOBD plasmids and selected on media lacking tryptophan and uracil (Sunrise Science Products, San Diego CA, 1316-030). Yeast strains were then mated and subsequently selected on medium lacking tryptophan ...
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... Cells were washed and stained with antibodies against cell surface molecules in PBS containing 4% of human serum (Valley Biomedical). NGFR was detected with a 20 μl per million cells of Alexa Flour 647 conjugated α-NGFR (BD Bioscience) ...