-
No products found
because this supplier's products are not listed.
Thomas Eng, et al.,
bioRxiv - Systems Biology 2021
Quote:
... 100 μL of DMSO extracted indigoidine was added to 96-well flat-bottomed microplates (Corning Life Science Products, Corning, NY). Indigoidine was quantitated by measuring the optical density at 612 nm wavelength (OD612 ...
-
No products found
because this supplier's products are not listed.
Sivasai Balivada, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Disposable RNAase-free pellet pestles (Bio Plas, Inc., San Rafael, CA, USA) were used for the homogenization of the tissue punches ...
-
No products found
because this supplier's products are not listed.
Giulia Monticone, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... the proteosomal inhibitor lactacystin (20 μM, Kamiya Biomedical Corp.) was added to the culture media for 8 hours before preparing protein lysates with the above lysis buffer supplemented with N-Ethylmaleimide (25mM ...
-
No products found
because this supplier's products are not listed.
Mark M. Smits, et al.,
bioRxiv - Physiology 2023
Quote:
... The NEP-inhibitor sacubitril (AHU-377; Nordic BioSite, Denmark) was dissolved in DMSO to a concentration of 10 mg/mL as stock solution ...
-
No products found
because this supplier's products are not listed.
Andrea Galisova, et al.,
bioRxiv - Bioengineering 2022
Quote:
... the free dye was removed by dialysis (GeBAflex-Midi Dialysis Tubes, 8kDa MWCO, Geba, Israel) against PBS buffer (16 h ...
-
No products found
because this supplier's products are not listed.
Austin Ferro, et al.,
bioRxiv - Neuroscience 2021
Quote:
Chow formulated to contain 1200 mg/kg free base Plexxikon 5622 (PLX; Chemgood, Inc.; Glen Allen, VA) was fed to mice between P18 and P27 ...
-
No products found
because this supplier's products are not listed.
Adele M. H. Seelke, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Animals were determined to be free of West Nile virus (WNV) via simian WNV ELISA (Xpress Bio), SIV-free ...
-
No products found
because this supplier's products are not listed.
Otmane Bouchatta, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The free-floating skin sections were stained and cleared with RapiClear 1.47 (Sunjin Lab, Hsinchu City, Taiwan), according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
William D. Leineweber, et al.,
bioRxiv - Cancer Biology 2024
Quote:
Free mitochondrial iron was measured using RPA according to the manufacturer’s protocol (Squarix Biotechnology GmbH, Marl, Germany). Cells were cultured in a glass-bottom 48-well plate and treated overnight with 10μM CoPP ...
-
No products found
because this supplier's products are not listed.
Rebecca L. Murray, et al.,
bioRxiv - Microbiology 2021
Quote:
CK2 was inactivated using the CK2-specific inhibitor CX-4945 (0308, Advanced Chemblocks). HeLa cells were seeded and transfected with CFP-VAP DNA the following day (for immunofluorescence assay only) ...
-
No products found
because this supplier's products are not listed.
Charlotte A. Dawson, et al.,
bioRxiv - Molecular Biology 2024
Quote:
Serine protease (SVSP) activity was determined using an absorbance-based Chromogenic S-2288 assay (Chromogenix, 82085239). Venoms were diluted to 0.066 mg/mL and 15 μL added per well in quadruplicate to a 384-well plate before incubation for 3 mins at 37 °C ...
-
No products found
because this supplier's products are not listed.
Amy R. Rappaport, et al.,
bioRxiv - Immunology 2021
Quote:
... Plates were incubated with anti-nucleocapsid protein primary antibody cocktail (clones HM1056 and HM1057) (EastCoast Bio, North Berwick, ME) for 60 minutes at 37°C (Battelle Memorial Institute ...
-
No products found
because this supplier's products are not listed.
Julia Stokes, et al.,
bioRxiv - Physiology 2020
Quote:
... Samples were spiked with a cocktail of heavy-isotope internal standards (Cambridge Isotope Laboratories, MA, USA; CDN Isotopes, Canada) and deproteinated with methanol ...
-
No products found
because this supplier's products are not listed.
Zian Liao, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... supplemented with 2% charcoal-stripped FBS and EPC cocktail (1 µM MPA, Sigma Cat#1378001- 200MG, 0.05 mM cAMP, Axxora Cat #JBS-NU-1502-50 ...
-
No products found
because this supplier's products are not listed.
Fanny Ehret, et al.,
bioRxiv - Neuroscience 2024
Quote:
... sections were extensively washed with PBS containing 0.1% Tween and incubated with secondary antibody cocktail containing Cy3 (713-165-147, Dianova) Dylight 405 (712-475-153 ...
-
No products found
because this supplier's products are not listed.
D. Brent Halling, et al.,
bioRxiv - Biophysics 2022
Quote:
... Ca2+ chelators were used in the intracellular solution to control free Ca2+ and (+)-(Crown-6)-2,3,11,12-tetracarboxylic acid (Synquest Laboratories) was used to chelate any contaminating barium to prevent barium block of channels ...
-
No products found
because this supplier's products are not listed.
Maria J. Garcia-Leon, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Serum free media or TA buffer were used as control together with PEG-treated 10µm polystyrene beads (Phosphorex). Aggregations were launched in aggregation vials by adding 200µl of washed platelets at a final platelet concentration of 3e5/µl ...
-
No products found
because this supplier's products are not listed.
Sungjin Park, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Click-IT reaction cocktail containing the Alexa Fluor® 488 azide or BP Fluor 555 azide (BroadPharm, Cat# BP-25564) was incubated for 30min in dark ...
-
No products found
because this supplier's products are not listed.
Adriana Bastías, et al.,
bioRxiv - Plant Biology 2019
Quote:
... once with tap water and the other with a modified Murashige & Skoog nitrogen-free basal medium (Phytotechnology Laboratories, M531), supplemented with 0.0 ...
-
No products found
because this supplier's products are not listed.
Yongchan Lee, et al.,
bioRxiv - Biochemistry 2023
Quote:
... laevis oocytes were performed in Na+-free ND96 buffer containing 50 μM of L-[14C]Leu (3.3CCiCmol−1, Moravek) for 30 min at room temperature ...
-
No products found
because this supplier's products are not listed.
Tomozumi Imamichi, et al.,
bioRxiv - Microbiology 2022
Quote:
... and anti-protease antibody (Cat# ab211627 Abcam, Cat# SKU: 65-018, As One International, Santa Clara, CA, USA), Protein bands were detected by using the ECL Prime Western Blotting Detection Reagent (MiliporeSigma ...
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No products found
because this supplier's products are not listed.
Łukasz Mazurek, et al.,
bioRxiv - Biochemistry 2020
Quote:
... 50 nM AlexaFluor488-labelled QnrB1 was mixed with 1 μM gyrase andand increasing concentration of label free linearised pBR322 (Inspiralis). The assays were carried out at specific excitation/emission wavelengths for Alexa-488 of 488/520 nm.
-
No products found
because this supplier's products are not listed.
Sebastian A. Pace, et al.,
bioRxiv - Neuroscience 2020
Quote:
... the tissue was incubated overnight in mouse polyclonal anti-CaMKIIα (1:200 in detergent-free blocking solution, GeneTex, Irvine, CA). The next day ...
-
No products found
because this supplier's products are not listed.
Nicola M. Blythe, et al.,
bioRxiv - Cell Biology 2019
Quote:
... cells were stretched whilst in serum-free medium using an FX-4000 or FX-5000 Flexercell Tension System (Flexcell International) to equibiaxially elongate the cell-seeded elastic membrane against a loading post ...
-
No products found
because this supplier's products are not listed.
Takahiro Ohde, Taro Mito, Teruyuki Niimi,
bioRxiv - Developmental Biology 2021
Quote:
... Total RNA from terga and embryos were eluted from a column with 20 µl of RNase-free water and submitted to GENEWIZ and Filgen standard RNA-seq service (GENEWIZ) that provided a standard Illumina mRNA library and generated 150 bp pair-end reads on an Illumina HiSeqX and HiSeq4000 (Illumina) ...
-
No products found
because this supplier's products are not listed.
Angelo Cardoso Batista, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... the Avestin EmulsiFlex-C3 homogeniser was used at 15000-20000 psi and the final pellet-free cell extract was dialysed (using a Spectra/Por 4 Dialysis Tubing, 12-14 kD MWCO from Repligen) overnight in S30B buffer before one final centrifugation at 12000xg for 30 minutes ...
-
No products found
because this supplier's products are not listed.
Alexandra Venuto, et al.,
bioRxiv - Neuroscience 2022
Quote:
... placed in 1.7 mL RNase-free microcentrifuge tubes containing 200 μl RNAzol (Molecular Research Center, Inc., OH. Catalog: RN 190) and homogenized immediately ...
-
No products found
because this supplier's products are not listed.
Gonghong Yan, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... and a tris-EDTA pH 9.0 buffer (made in house) for 30 minutes in the IHC-TekTM Epitope Retrieval Steamer (IHC World. Catalog No. IW-1102). The primary mouse monoclonal anti-BCL2 antibody was incubated at 1:400 dilution ...
-
No products found
because this supplier's products are not listed.
Miguel A. Olivencia, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
25-hydroxyvitamin D and testosterone levels were measured in rat citrated plasma and human EDTA plasma using a General 25-Hydroxyvitamin D3 (HVD3) ELISA Kit (Reddot Biotech Inc., Kelowna, Canada) and Testosterone ELISA DE1559 (Demeditec ...
-
No products found
because this supplier's products are not listed.
William C Davis, et al.,
bioRxiv - Immunology 2019
Quote:
... and 100 μg/mL of streptomycin sulfate] in the presence of a DC growth cocktail containing bovine GM-CSF and IL-4 (Kingfisher Biotech, MN). On the third day ...
-
No products found
because this supplier's products are not listed.
Luis F. Garcia-Alles, et al.,
bioRxiv - Biochemistry 2019
Quote:
... protein tags were removed by incubation overnight at 25°C with turbo TEV protease (GenWay, 5 μg/mL final) in 50 mM Tris pH 8.0 / 300 mM NaCl /2 mM DTT/ 1 mM EDTA ...
-
No products found
because this supplier's products are not listed.
W. Bai, et al.,
bioRxiv - Bioengineering 2020
Quote:
... 1 mL of the cell-free liquid phase was added to 15 mL helium-flushed septum-capped glass vials (Exetainer, Labco, Houston) containing 1 mL 85% phosphoric acid ...
-
No products found
because this supplier's products are not listed.
Rahia Mashoodh, et al.,
bioRxiv - Molecular Biology 2021
Quote:
Mutant and corresponding control ES cells were generated from single blastocyst using feeder-free-based 2i LIF culture conditions (N2B27, Stem Cell Sciences) (30) ...
-
No products found
because this supplier's products are not listed.
Marta Napiorkowska, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... 30 min) and the cell-free extract was loaded onto column packed with 2 mL Super Ni-NTA matrix (Protein Ark) pre-equilibrated with 3 column volumes (CV ...
-
No products found
because this supplier's products are not listed.
Dinesh K Deochand, et al.,
bioRxiv - Immunology 2024
Quote:
Macrophages were crosslinked with 1% methanol-free formaldehyde (Thermo) for 10 min (H3K27ac) or double-crosslinked with 2 mM disuccinimidyl glutamate (Proteochem, c1104) for 30 min followed by 1% methanol-free formaldehyde for 10 min at RT (GR ...
-
No products found
because this supplier's products are not listed.
de la O Sean, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... + 0.22 g glucose (MilliporeSigma, G7528) + 1.23 g sodium bicarbonate (MilliporeSigma, S5761) + 10 g fatty-acid free bovine serum albumin (FAF-BSA) (Lampire Biological Laboratories, 7500812) + 10 μL Insulin-Transferrin-Selenium-Ethanolamine (ITS-X ...
-
No products found
because this supplier's products are not listed.
Pedro Pinto, et al.,
bioRxiv - Microbiology 2021
Quote:
... Nuclease-free water and gDNA extracted from 106 of Cryptosporidium parvum IOWA strain purified oocysts (Waterborne™, Inc., New Orleans, LA, USA) were used as negative and positive control ...
-
No products found
because this supplier's products are not listed.
C Wang, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... Wells were washed with PBS three times and a cell-free gap was created with a single pass of a 10-μl pipette tip (F1732031, Gilson, Middleton, WI). Floating cells were gently washed away with pre-warmed media ...
-
No products found
because this supplier's products are not listed.
N Romanò, et al.,
bioRxiv - Physiology 2020
Quote:
... The eluate was dried down under a stream of heated oxygen free nitrogen (OFN, 40°C) on an SPE DryTM Dual Sample Concentrator System (Biotage, Uppsala, Sweden). Once dry the extracts were dissolved in water/methanol (70:30 ...
-
No products found
because this supplier's products are not listed.
Emiel Vanhulle, et al.,
bioRxiv - Microbiology 2022
Quote:
... serum-containing medium was replaced by serum-free DMEM and cells were inoculated with MOI 3 of VSVΔG*/Luc-G (Kerafast, Boston, MA, USA) for the production of luciferase-expressing pseudotypes ...
-
No products found
because this supplier's products are not listed.
Amira Metwaly, et al.,
bioRxiv - Microbiology 2022
Quote:
... Each germ-free recipient mouse received 100 µL of the suspension via oral gavage using a 20 Gauge gavage needle (Fine Science Tools). Mice were sacrificed 4 weeks after colonization ...
-
No products found
K. Jerabkova-Roda, et al.,
bioRxiv - Cell Biology 2023
Quote:
... melanoma cell lines at 3×105 cells/ml were labelled with Hoechst 33342 and suspended in 3mg/mL of serum free solution of neutralized Type I Bovine Collagen (PureCol® 5005-B, Advanced Biomatrix). Then ...
-
No products found
because this supplier's products are not listed.
Omar Al Rifai, et al.,
bioRxiv - Physiology 2021
Quote:
Intact and C-terminal FGF23 measurements were performed on EDTA plasma collected from mice under fed conditions using ELISA assay specific for mouse intact or C-terminal FGF23 (Quidel, cat#: 60-6800 and cat#: 60-6300). Since C-terminal FGF23 ELISA detects both intact and processed FGF23 ...
-
No products found
because this supplier's products are not listed.
Luyang Xiong, et al.,
bioRxiv - Immunology 2023
Quote:
... After 24 hours cells were washed with 1x D-PBS and incubated in HFSC Serum Free Un-differentiation Media (Celprogen cat.# M36007-08U) overnight ...
-
No products found
because this supplier's products are not listed.
Boyang Zhao, et al.,
bioRxiv - Biochemistry 2023
Quote:
... and σNS-ΔN17 were subcloned into the bacterial expression vector pET28 with an N-terminal His tag and a TEV protease cleavage site (Epoch Life Science). Escherichia coli DE3 cells (Novagen ...
-
No products found
because this supplier's products are not listed.
M Sadeq Saleh, et al.,
bioRxiv - Neuroscience 2019
Quote:
Gold electroplating was carried out using in Earthcoat Cyanide Free Gold Plating Solution (24K) heated to 60 °C on a hot plate with stirrer (Model MS300HS, Medline Scientific, Oxon, United Kingdom). The solution (filled to an adequate depth for submersion in a 1 L thick glass beaker ...
-
No products found
because this supplier's products are not listed.
Erin A. Nekritz, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... MiRNA inhibition in MCF-10A cells was achieved by transducing cells with a miRNA inhibitor against hsa-miR-424-5p and hsa-miR-503-5p (Genecopoeia #HmiR-AN0494-AM03 and #HmiR-AN0550-AM03 respectively) ...
-
No products found
because this supplier's products are not listed.
Elena Sánchez Martín-Fontecha, et al.,
bioRxiv - Plant Biology 2024
Quote:
Seven-dpg seedlings grown horizontally in MS medium were pre-treated with the proteasome inhibitors 50 μM MG132 and 20 nM epoxomicin for 1 h and then with 10 μM GR24rac (Strigolab) for further 3 h at 22°C ...
-
No products found
because this supplier's products are not listed.
Yuhan Jiang, et al.,
bioRxiv - Biochemistry 2023
Quote:
... 75 µL of the cell lysate was mixed with 100 µL of 70% nitric acid (Fisher chemical, Cat# A509P212) in 15 mL metal-free tube (Labcon, Cat# 3134-345-001-9). The samples were heated in 60°C water bath for 1 h to make sure the sample is fully digested ...
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Daniel C. Levine, et al.,
bioRxiv - Neuroscience 2024
Quote:
... hypothalamus from PER2-TgWT mice that were fasted for 16 hours or given ad libitum access to HFD for 1 week was excised at ZT16 and extracted with ∼5 volumes of strong RIPA buffer containing kinase and phosphatase inhibitors (Abbexa abx090624), sonicated in a water bath 3 x 30sec on high ...