-
No products found
because this supplier's products are not listed.
Yuhan Jiang, et al.,
bioRxiv - Biochemistry 2023
Quote:
... 75 µL of the cell lysate was mixed with 100 µL of 70% nitric acid (Fisher chemical, Cat# A509P212) in 15 mL metal-free tube (Labcon, Cat# 3134-345-001-9). The samples were heated in 60°C water bath for 1 h to make sure the sample is fully digested ...
-
No products found
because this supplier's products are not listed.
Ana Uzquiano, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Organoids from day 90 were dissociated using spring scissors (Roboz, RS-5650), while all others were dissociated by gentle pipetting ...
-
No products found
because this supplier's products are not listed.
Canan Celiker, et al.,
bioRxiv - Cell Biology 2023
Quote:
... solubilized in PCR grade water (Top-Bio). For RT-qPCR ...
-
No products found
because this supplier's products are not listed.
Tomasz Zieliński, et al.,
bioRxiv - Biophysics 2022
Quote:
... cells were incubated at 37◦C in 95% air/5% CO2 atmosphere in the CO2 incubator (Nuaire) for 2 h ...
-
No products found
because this supplier's products are not listed.
Juan A Perez-Bermejo, et al.,
bioRxiv - Genetics 2021
Quote:
... High purity iPS-CM cultures (iCell Cardiomyocytes2, Lot#CMC331743, >99% cTnT+) were obtained from Cellular Dynamics International (Madison ...
-
No products found
because this supplier's products are not listed.
Fátima Sanchís-Calleja, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... using paired-end 28/10/10/90 or 28/8/0/91 configuration (Read1/IDX i7/IDX i5/Read2). After sample demultiplexing ...
-
No products found
because this supplier's products are not listed.
Esther Cañibano, et al.,
bioRxiv - Plant Biology 2020
Quote:
... 2 ug total RNA extracted from 7 day old seedlings with the Favorprep Plant Total RNA Purification Mini kit (Favorgen) was used for cDNAs synthesis with using the High-Capacity cDNA Reverse Transcription kit (Applied Biosystems ...
-
No products found
because this supplier's products are not listed.
Richard J. R. Kelwick, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
Nanoparticle tracking analysis (NTA): 1 μL A549 EVs (1 mg/ml; #HBM-A549-100/5, HansaBioMed, Tallinn, Estonia) were diluted (1:1000 ...
-
No products found
because this supplier's products are not listed.
Akhil A. Vinithakumari, et al.,
bioRxiv - Neuroscience 2021
Quote:
... and 90% nitrogen at 37°C) and incubated in an anaerobic workstation (AS-580, Anaerobe Systems, Morgan Hill, CA, USA). Primary C ...
-
No products found
because this supplier's products are not listed.
D. Hoving, et al.,
bioRxiv - Immunology 2023
Quote:
... serum from a donor 3 weeks post-PCV13 vaccination was diluted 1:100 in 0.5% BSA in PBS with 10µg/mL cell wall PS multi (CWPS-multi; SSI Diagnostica; 68866 ...
-
No products found
because this supplier's products are not listed.
Krista K. Alexander, et al.,
bioRxiv - Biochemistry 2023
Quote:
Peptides were diluted in water and were analyzed using a 1.00 mm QS cuvette (Starna Cells). A JASCO J-710 circular dichroism spectrometer and JASCO Spectra Manager software were used to analyze the results using the following parameters ...
-
No products found
because this supplier's products are not listed.
Arman Izadi, et al.,
bioRxiv - Immunology 2023
Quote:
... XBB (#158-40589-V08H40-100) and BQ1.1(158-40589-V08H41-100) was aquired from Nordic biosite. 25 µg of Spike protein was biotinylated according to the instructions of EZ-LinkTM Micro Sulfo-NHS-LCBiotinylation Kit (ThermoFisher) ...
-
No products found
because this supplier's products are not listed.
Fumiyuki Hatanaka, et al.,
bioRxiv - Bioengineering 2023
Quote:
... 1:100 (MCA-1B7, EnCor Biotechnology); anti-ChAT ...
-
No products found
because this supplier's products are not listed.
Jayanth S. Shankara Narayanan, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... anti-Rat HRP Polymer (Cell IDX, 2AH-100) or anti-Rabbit HRP Polymer (Cell IDX ...
-
No products found
because this supplier's products are not listed.
Jiachen Huang, Darren Diaz, Jarrod J. Mousa,
bioRxiv - Immunology 2020
Quote:
... Plates were washed with water three times before applying 25 μl secondary antibody (goat anti-human IgG Fc; Meridian Life Science) at a dilution of 1:4,000 in blocking solution ...
-
No products found
because this supplier's products are not listed.
Alan P. R. Lorenzetti, et al.,
bioRxiv - Systems Biology 2022
Quote:
... Cell pellets were resuspended in Milli-Q water and disrupted at 4°C using ceramic beads (Mo Bio Laboratories) and a Precellys 24 homogenizer (Bertin Corp). Protein content was determined by bicinchoninic acid assay (BCA ...
-
No products found
because this supplier's products are not listed.
Elvira Nikalayevich, et al.,
bioRxiv - Cell Biology 2021
Quote:
... The following antibodies were used at the indicated concentrations: Human CREST serum (Immunovision, HCT-100, at 1:50 or 1:100), Rabbit poly clonal anti-Rec8 (gift from Scott Keeney ...
-
No products found
because this supplier's products are not listed.
Rudolf O. Schlechter, et al.,
bioRxiv - Microbiology 2023
Quote:
... gas permeability of 0.6 m3 m−2 day−1 and water loss of 1 g m−2 day−1; Brooks Life Sciences, UK), and incubated at 30°C with shaking ...
-
No products found
because this supplier's products are not listed.
Nobuyuki Oguri, et al.,
bioRxiv - Pathology 2023
Quote:
... and 100 mM Phosphate (FUJIFILM Wako Pure Chemical Corp.)-NaOH (pH 7.4) with 150 μM H-D-prolyl-L-phenylalanyl-Larginine-p-nitroaniline dihydrochloride (Chromogenix S-2302, Diapharma).
-
No products found
because this supplier's products are not listed.
Soumyanetra Chandra, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Reads were converted into FASTA format and aligned with the ccdA gene sequence using the WATER program of the EMBOSS package (Rice et al. 2000; Carver and Bleasby 2003) Default values were used for all the parameters except the Gap Opening Penalty ...
-
No products found
because this supplier's products are not listed.
Siddhartha Banerjee, Ayanjeet Ghosh,
bioRxiv - Biophysics 2021
Quote:
1 mg/ml Tau-441 (rPeptide, USA) in the buffer containing 50mM MES ...
-
No products found
because this supplier's products are not listed.
Declan J. Lafferty, et al.,
bioRxiv - Plant Biology 2023
Quote:
... The tissue was filtered using a 100 µM cell strainer (C4100, MTC Bio, USA) and washed with 50 mL sterile deionized water ...
-
No products found
because this supplier's products are not listed.
Miguel Pineda, et al.,
bioRxiv - Immunology 2021
Quote:
... Collagen-Induced Arthritis (CIA) was induced with bovine type II Collagen (MD Biosciences, 100 mg), injected intradermally (day 0 ...
-
No products found
because this supplier's products are not listed.
Michael S. Balzer, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... and 10 U/ml recombinant mouse interferon-□ (Cell Sciences, Canton, MA) at 33 °C (permissive conditions ...
-
No products found
because this supplier's products are not listed.
Neil G. Rumachik, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... 100-mM Dithiothreitol (DTT) in water (BIO-WORLD Cat#404200001) was then added to each tube to a final concentration of 5-mM ...
-
No products found
because this supplier's products are not listed.
Yuxiao Guo, et al.,
bioRxiv - Physiology 2021
Quote:
An active recording sEMG electrode (disposable self-adhering Ag-AgCl electrodes; 95 mm2, Ambu Neuroline ...
-
No products found
because this supplier's products are not listed.
Benjamin M. David, Paul A. Jensen,
bioRxiv - Systems Biology 2022
Quote:
... Samples were homogenized for 3 minutes in two 90 s intervals at 1600 rpm (OHAUS homogenizer), then heated at 65 °C for 5 minutes ...
-
No products found
because this supplier's products are not listed.
Tomer Milo, et al.,
bioRxiv - Systems Biology 2023
Quote:
... Samples were reconstituted in 10% methanol and 90% assay buffer provided by the kit manufacturer and cortisol was quantified using competitive Enzyme-Linked Immunosorbent Assays (ELISA; Salimetrics Europe ...
-
No products found
because this supplier's products are not listed.
Élora Midavaine, et al.,
bioRxiv - Neuroscience 2023
Quote:
... coupling reagents (ChemImpex International) and N-methylpyrrolidinone (A&C American Chemicals Ltd) were HPLC grade ...
-
No products found
because this supplier's products are not listed.
Toshiharu Ichinose, et al.,
bioRxiv - Neuroscience 2024
Quote:
... The ribosome-bound mRNA was eluted with 50 µl of 100 µg/ml 3× FLAG peptide (GEN-3XFLAG- 25, Protein Ark) dissolved in the lysis buffer.
-
No products found
because this supplier's products are not listed.
Cecilia S. Blengini, et al.,
bioRxiv - Cell Biology 2021
Quote:
... phosphorylated CDC25B (1:100; Signalway Antibodies #11949), γ-tubulin (1:100 ...
-
No products found
because this supplier's products are not listed.
Barbara Eckmair, et al.,
bioRxiv - Biochemistry 2023
Quote:
... The MAPA-glycans were diluted to 100 µM in spotting buffer (100 mM Sodium phosphate, pH 8.5) and printed using a sciFLEXARRAYER S11 (Scienion, Germany) on NHS activated glass slides (SCHOTT Nexterion ...
-
No products found
because this supplier's products are not listed.
Shelei Pan, et al.,
bioRxiv - Neuroscience 2021
Quote:
... 30%) to double distilled water (DDW) following xylene and immersed in FD Cresyl Violet Solution (#PS102-02, FD NeuroTechnologies, Columbia, MD) for 5 minutes ...
-
No products found
because this supplier's products are not listed.
Abdallah W. Abdelhady, et al.,
bioRxiv - Biophysics 2023
Quote:
... oocytes were placed with as little surrounding vitrification solution as possible onto one of three polymer sample supports (Figure S2): a 10 μm thick cryocrystallography sample support with a 100 μm aperture (MicroLoop LD 100, MiTeGen, Ithaca, NY), a flat ...
-
No products found
because this supplier's products are not listed.
Xiuping Sun, Bing Chen, Zhiwei Song, Lei Lu,
bioRxiv - Cell Biology 2021
Quote:
... Bafilomycin A1 (working concentration: 100 nM) was from Chemscene.
-
No products found
because this supplier's products are not listed.
Renato M. Rossi, et al.,
bioRxiv - Immunology 2022
Quote:
... Samples of all total mixed rations were collected at 2-weeks intervals from the feed bunk at the time of distribution and sent to an external laboratory for chemical composition analysis (Cumberland Valley Analytical Services, Waynesboro, PA). The composition and chemical analysis results of the diets are summarized in Supplementary Tables S1 and S2.
-
No products found
because this supplier's products are not listed.
Hua He, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... Rabbit anti-ABCA3 (Seven Hills Bioreagents WRAB-70565; 1:100), Rabbit anti-NKX2.1 (Seven Hills Bioreagents WRAB-1231 ...
-
No products found
because this supplier's products are not listed.
Yuxuan Han, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... were cultured in Roswell Park Memorial Institute medium (RPMI-1640, Fujifilm Wako Pure Chemical Corporation, Osaka, Japan) supplemented with 10% fetal bovine serum (FBS; Nichirei Biosciences Inc., Tokyo, Japan), 100 U/mL penicillin (Meiji-Seika Pharma Co. ...
-
No products found
because this supplier's products are not listed.
Elisa Tonoli, et al.,
bioRxiv - Neuroscience 2021
Quote:
... 30 μg/ml TG2 (Zedira) in ACSF was perfused until plateau of the response was reached ...
-
No products found
because this supplier's products are not listed.
Mayra Martinez-Lopez, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... Mitomycin C(0.5mg/mL; Medac)/L-PBS or Mitomycin C/L-Clodronate were injected intratumorally and xenografts were placed immediately in clean E3 medium ...
-
No products found
because this supplier's products are not listed.
Zachary T. Olmsted, et al.,
bioRxiv - Neuroscience 2020
Quote:
... We further compared neurons cultured in patterning versus maturation medium using 5 μl/ml BDNF and 5 μl/ml GDNF slow release PLGA microbeads (StemCultures; 5 ng/ml steady-state concentrations) at two time points ...
-
No products found
because this supplier's products are not listed.
Richard J. Burt, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... anti-dsRNA antibody J2 (1ug/ml) (SCICONS)was added at 1:100 dilution in 1% BSA followed by DyLight® 488 Anti-Mouse IgG (H+L ...
-
No products found
because this supplier's products are not listed.
Albert S. W. Kang, et al.,
bioRxiv - Biophysics 2020
Quote:
... Purification from excess OsBp was done with spin columns (TC-100 FC from TrimGen Corporation) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Catherine S. Millar-Haskell, et al.,
bioRxiv - Bioengineering 2022
Quote:
ECM proteins were covalently attached to PAA using the crosslinker Sulfo-SANPAH (Covachem; 13414-100). To begin ...
-
No products found
because this supplier's products are not listed.
Adela Constantinescu-Bercu, et al.,
bioRxiv - Cell Biology 2020
Quote:
... blood was collected retro-orbitally from GpIbαΔsig/Δsig mice and wild-type littermates in 100µg/ml Hirudin (Refludan, CSL Behring GmbH) and labelled with Dy-Light 488-conjugated rat anti-mouse GPIbβ (Emfret Analytics, 6µg/ml). When indicated ...
-
No products found
because this supplier's products are not listed.
Nathan M. Belliveau, et al.,
bioRxiv - Cell Biology 2022
Quote:
... resuspended in 10 mL PolymorphPrep (Cosmo Bio USA #AXS1114683) and added to the bottom of a 50 mL conical tube ...
-
No products found
because this supplier's products are not listed.
Ragna S. Häussler, et al.,
bioRxiv - Biochemistry 2019
Quote:
... the antibody-coupled beads were washed three times in 100 μl 1x PBS (09-9400, Medicago), 0.05% Tween20 (BP337 ...
-
No products found
because this supplier's products are not listed.
Michelle El-Khoury, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Rabbit anti-GFP (TP401) (Immunofluorescence: 1:100) was obtained from Torrey Pines Biolabs (Secaucus, NJ, USA).
-
No products found
because this supplier's products are not listed.
Matthias Hecht, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 50,000 cells were resuspended in 100 µl prewarmed DMEM without FBS and transferred into a 8 µM PET tissue culture plate insert (BrandTech). The insert was placed into a well of a 24 well cell culture plate containing 700 µl prewarmed DMEM supplemented with 10 % FBS ...
-
No products found
because this supplier's products are not listed.
Laurent M. Paardekooper, et al.,
bioRxiv - Immunology 2023
Quote:
... At least 10·106 cells were stained for 30 minutes in the dark in 100 µl (75 μl EuroFlow B cell tube mix and 25 μl Cytognos isotype mix) staining solution according to the EuroFlow SOP for sample preparation and staining of markers followed by immediate analysis (www.EuroFlow.org).