-
No products found
because this supplier's products are not listed.
Doris Krauter, et al.,
bioRxiv - Neuroscience 2021
Quote:
... using a diamond knife (Histo HI 4317, Diatome). Afterwards sections were stained according to Gallyas52 and with Methylene blue/ Azur II for 1 min ...
-
No products found
because this supplier's products are not listed.
Julian Schöllkopf, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
N-terminally His-tagged Pasteurella multocida toxin (PMT) was coated at 10 µg/mL (500 ng/well ...
-
No products found
because this supplier's products are not listed.
Chafen Lu, et al.,
bioRxiv - Microbiology 2019
Quote:
... Secondary antibodies were rabbit polyclonal anti-His (Delta Biolabs), Horseradish peroxidase (HRP)-anti-rabbit and HRP-anti-mouse IgG (GE Healthcare ...
-
No products found
because this supplier's products are not listed.
Martina Oravcová, et al.,
bioRxiv - Cell Biology 2022
Quote:
Endogenous level of DNA damage in HEK293 cells was evaluated using the CometAssay kit (Trevigen) according the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
David A. Yarmolinsky, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Laminectomy was performed (typically at lamina T13 and L1) by careful thinning of the lateral edge of the laminae using a high-speed micro-drill (Roboz) driving 0.05mm tungsten carbide burrs (Fine Science Tools) ...
-
No products found
because this supplier's products are not listed.
Ole A.W. Haabeth, et al.,
bioRxiv - Immunology 2021
Quote:
... 2 μg of the RBD-his mRNA or GFP mRNA (Trilink) was formulated in 6.6 μl of PBS pH 5.5 with 1.37 μl of 5mM CART and added to the cells ...
-
No products found
because this supplier's products are not listed.
Huang Zhu, Dan S. Kaufman,
bioRxiv - Immunology 2019
Quote:
... 15 % heat-inactivated human AB serum (Valley Biomedical, Catalog # HP1022 HI),1 % P/S ...
-
No products found
because this supplier's products are not listed.
Aleksei Kuznetsov, et al.,
bioRxiv - Biochemistry 2020
Quote:
Human recombinant ACE2-His protein (Icosagen OÜ, Estonia, cat# P-302-100) and SARS-CoV-2 Spike protein S1 (Icosagen OÜ ...
-
No products found
because this supplier's products are not listed.
Shemin Mansuri, et al.,
bioRxiv - Cell Biology 2023
Quote:
The human post-mortem thalamus sections of a Parkinson’s Disease (PD) patient (BioChain Institute Inc., USA Cat# T2236079Par) and a control (BioChain Institute Inc. ...
-
No products found
because this supplier's products are not listed.
KM Smith, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Either parasagittal or transverse slices were prepared (L1-L5, 200µm thick: LI-L5 300μm thick, respectively) both using a vibrating microtome (Campden Instruments 7000 smz, Loughborough, UK). Targeted CR+ and unidentified recordings were undertaken in parasagittal slices ...
-
No products found
because this supplier's products are not listed.
Janet H.T. Song, et al.,
bioRxiv - Neuroscience 2022
Quote:
... were deposited on each side of the midline at L1-L2 using a pulled glass micropipette with a nanojector (Nanoject II, Drummond Scientific, Broomall, PA) to obtain bilateral labeling ...
-
No products found
because this supplier's products are not listed.
Camden R. Bair, et al.,
bioRxiv - Microbiology 2021
Quote:
... supplemented with 8% heat-inactivated newborn calf serum (HI-NBCS, Rocky Mountain Biologicals), 1.5 g/ml Na2CO3 (Amresco) ...
-
No products found
because this supplier's products are not listed.
Wanda M. Figueroa-Cuilan, et al.,
bioRxiv - Microbiology 2022
Quote:
... Hi-FBS was achieved by placing the FBS (Atlas Biologicals, FP-0500-A) in a water bath at ∼56 °C for 30 minutes ...
-
No products found
because this supplier's products are not listed.
Sumona P. Dhara, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Reactions were quenched in heat inactivated fetal calf serum (HI-FCS; Gemini Bio-Products) in DMEM/F12 (Gibco ...
-
No products found
because this supplier's products are not listed.
Yu Jung Shin, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Microvessel layers were sputter coated with Au/Pd alloy (Denton Desk IV, Denton Vacuum) and imaged using a JSM 6610 LV scanning electron microscope (JEOL) at an accelerating voltage of 5kV.
-
No products found
because this supplier's products are not listed.
Neil J Rzechorzek, et al.,
bioRxiv - Biochemistry 2020
Quote:
... HEK293-F cells were grown in 400 mL batches in 2 L non-baffled Erlenmeyer flasks (TriForest Enterprises Inc, #FPC2000S) to a cell density of ∼1×106 cells/mL.
-
No products found
because this supplier's products are not listed.
Justine Creff, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 200 µg for HEK293) were incubated with 3 µg of the indicated antibodies and 12 µL protein sepharose beads (IPA300, Repligen) at 4°C for 4 h ...
-
No products found
because this supplier's products are not listed.
Peng Zhang, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Keigi Fujiwara (University of Texas MD Anderson Cancer Center, Houston, TX) and IP3R1-null HEK293 cells (Kerafast, Inc., Boston, MA) (Alzayady ...
-
No products found
because this supplier's products are not listed.
Shanshan Lang, et al.,
bioRxiv - Bioengineering 2019
Quote:
... The recombinant extracellular domain of CD38 and PD-L1 was produced as a 6-His and AviTag™ fusion in HEK293 cells and purified by Ni-NTA followed by in vitro biotinylation by BirA biotin ligase (Avidity).
-
No products found
because this supplier's products are not listed.
Anne Rehkamp, et al.,
bioRxiv - Biochemistry 2020
Quote:
LC separation of peptides originating from HEK293 cell membranes was performed via selfpacked C18 columns (PicoFrits, 75 μm x 50 cm, 15 μm tip, New Objective, self-packed with ReproSil-Pur 120 C18-AQ ...
-
No products found
because this supplier's products are not listed.
Katrin Manske, et al.,
bioRxiv - Bioengineering 2023
Quote:
20,000 Human Embryonic Kidney cells (HEK293-CD19+) expressing the CD19 antigen were seeded on a 96-well E-plate (ACEA Biosciences) over night ...
-
No products found
because this supplier's products are not listed.
Ronan Shaughnessy, et al.,
bioRxiv - Cell Biology 2023
Quote:
... lentivi-ral particles were produced in the HEK293 FT packaging cells transfected with MiniBAR constructs and the Excelenti LTX Lentivirus Packaging mix (Oxford Genetics) using lipo-fectamine following manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Meenakshi Basu Shrivastava, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 500 ng human recombinant His-tagged ubiquitin-conjugating enzyme (E2) Ube2d3 (from BIOMOL International, #U0880), in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Rahul Kumar, et al.,
bioRxiv - Cell Biology 2023
Quote:
GST-MICAL-L1-CC protein was expressed in Escherichia coli BL21 (500 μM isopropyl β-d-1-thiogalactopyranoside; Wisent Bioproducts; at room temperature for 16 h) and purified using standard procedure in Tris buffer (20 mM Tris ...
-
No products found
because this supplier's products are not listed.
Elena Arutyunova, et al.,
bioRxiv - Biophysics 2021
Quote:
... The fusion protein was subsequently digested with His-tagged SUMO protease (McLab, South San Francisco, CA) at 4 °C for 1–2 h to remove the SUMO tag and the resulting cleavage mixture was then passed through Ni-NTA resin column ...
-
No products found
because this supplier's products are not listed.
J. Cody Herron, et al.,
bioRxiv - Systems Biology 2022
Quote:
... supplemented with 2% HI-FBS and 20 μM Rho kinase inhibitor Y-27632 (Hello Bio, HB2297) for 25 min of inhibition during frustrated phagocytosis ...
-
No products found
because this supplier's products are not listed.
Ian Dardani, et al.,
bioRxiv - Bioengineering 2022
Quote:
... mice were continuously fed a diet with BRAF/MEK inhibitors (PLX4720 200ppm + PD-0325901 7ppm, chemical additive diet, Research Diets, New Brunswick, NJ). Tumor size was assessed once weekly by caliper measurements (length x width2/2) ...
-
No products found
because this supplier's products are not listed.
Ozan Çiftçi, et al.,
bioRxiv - Genomics 2022
Quote:
... Cleaned frustules were dried onto aluminum stubs and coated with a layer of platinum-palladium (Pt 80%, Pd 20%) in a Quorum Q150TS sputter coater (Quorum Technologies Ltd., UK). Four strains (DCG0091 ...
-
No products found
because this supplier's products are not listed.
Eirini Maniou, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... Pharmacological inhibitors pan-FGFRi (BGJ 398, Generon, used at 1 μM) or FGFR1-targeting inhibitor (PD 173074, Cambridge Bioscience, used at 1 μM) were dissolved in DMSO and added to the rat serum at the start of culture ...
-
No products found
because this supplier's products are not listed.
Bjoern Traenkle, et al.,
bioRxiv - Immunology 2021
Quote:
... an alpaca (Vicugna pacos) was immunized with the purified extracellular domains of human CD4 (aa26-390) recombinantly produced in HEK293 cells (antibodies-online GmbH, Germany). After initial priming with 1 mg ...
-
No products found
because this supplier's products are not listed.
Nikhil J. Parekh, et al.,
bioRxiv - Immunology 2019
Quote:
IFN-β protein was detected using the mouse Hi-Sensitivity IFN-β ELISA kit (PBL Assay Science). CCL4 protein was detected using the mouse CCL4/MIP-1 beta Quantikine ELISA kit (R&D Systems) ...
-
No products found
because this supplier's products are not listed.
Mohd Farid Abdul Halim, et al.,
bioRxiv - Microbiology 2023
Quote:
... maripaludis MM1265 or Δfdh1 Δfdh2 expressing fdh2-His were grown in 1-liter anaerobic Schott bottles (Chemglass) containing McCas-formate medium with a N2:CO2 (80:20 ...
-
No products found
because this supplier's products are not listed.
Yixin Xu, et al.,
bioRxiv - Biophysics 2023
Quote:
... 0.5 μL L-PEG-L (8 mg/ml) and 0.5 μL Pd(NO3)2 (1 mg/ml) was applied to holey carbon grid (Quantifoil 200 mesh Cu R2/2) which was glow discharged 45 s at 15 mA ...
-
No products found
because this supplier's products are not listed.
José Miguel Arcas, et al.,
bioRxiv - Neuroscience 2024
Quote:
Whole-cell voltage- and current-clamp recordings were obtained from mouse DRG neurons or transiently transfected HEK293 cells with borosilicate glass patch-pipettes (Sutter Instruments, 4-8 MΩ resistance) and were performed simultaneously with temperature recordings ...
-
No products found
because this supplier's products are not listed.
Yunye Zhu, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Transformants scraped from densely plated transformation plates were inoculated into fresh SC-His medium with 2% raffinose (Amresco, J392) at 0.25 x 107 cells/ml and grown until 0.5-0.8 x 107 cells/ml ...
-
No products found
because this supplier's products are not listed.
Cara Abecunas, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... The following secondary antibody with specified source and catalogue number was used at 1:10000 dilution for Western blot PD assays: horseradish peroxidase-conjugated anti-rabbit antibody (AB_2313567) (rabbit IgG, Jackson ImmunoResearch Laboratories Inc., Cat# 111-035-003).
-
No products found
because this supplier's products are not listed.
Raveen Rathnasinghe, et al.,
bioRxiv - Microbiology 2021
Quote:
... were maintained in Dulbecco’s Modified Eagle Medium (DMEM) complemented with 10% heat-inactivated Fetal Bovine Serum (HI-FBS; PEAK serum), penicillin-streptomycin (Gibco ...
-
No products found
because this supplier's products are not listed.
Aman Y. Husbands, et al.,
bioRxiv - Plant Biology 2022
Quote:
... Membranes were washed three times with PBS-T and incubated with 1:2000 anti-His-tag antibody (Abiocode M0335-1) for 1h with gentle shaking at RT ...
-
No products found
because this supplier's products are not listed.
Cunxi Wang, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... The Mpp75Aa1.1-His fractions identified by SDS-PAGE were pooled and further processed using EndoTrap® HD (Hyglos GmbH, Germany) to reduce endotoxin contents (manufacturer’s instruction) ...
-
No products found
because this supplier's products are not listed.
MEENAKSHI TETORYA, et al.,
bioRxiv - Plant Biology 2023
Quote:
... The His-tagged peptides (GMA4CG_WT and GMA4CG_V6) phospholipid binding assays were performed using the PIP StripsTM (Echelon Biosciences Inc., CA) as previously described (Islam et al. ...
-
No products found
because this supplier's products are not listed.
Rui Fujii, et al.,
bioRxiv - Cell Biology 2024
Quote:
Sch91–183–6xHis was detected using mouse monoclonal anti-His antibody (AB10597733, cat. # D291–3; MBL International, Woburn, MA, USA). A C-terminal fragment of Sch9–5HA was detected using a mouse monoclonal anti-HA antibody (AB2565007 ...
-
No products found
because this supplier's products are not listed.
Nirupa Nagaratnam, et al.,
bioRxiv - Biochemistry 2022
Quote:
... The supernatant containing detergent solubilized His-tagged FopA was filtered through a 0.22 μm cellulose acetate membrane filter (Foxx Life Sciences) and loaded at 1 mL/min onto a Ni-NTA column (HisTrap HP ...
-
No products found
because this supplier's products are not listed.
Asha A. Philip, John T. Patton,
bioRxiv - Microbiology 2022
Quote:
... A puc19 plasmid containing a RIX/NSP3-2A-P-His insert under the control of a T7 transcription promoter (puc19/T7/ RIX/NSP3-2A-P-His) was purchased from Bio Basic Canada Inc ...
-
No products found
because this supplier's products are not listed.
Yunlong Li, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... the full-length codon optimized gene with N-terminal 6x-His-sequnce and NdeI-XhoI sites was synthesized (IDT DNA Inc.) (Table S2) ...
-
No products found
because this supplier's products are not listed.
Shahrnaz Kemal, et al.,
bioRxiv - Neuroscience 2023
Quote:
... All vectors contain an N-terminal 6x-His-tag, C terminal Strep-Tag II tag, with or without a N or C terminal fluorophore (EGFP, mNeonGreen (Allele Biotech), or mRuby2).
-
No products found
because this supplier's products are not listed.
Oskar Staufer, et al.,
bioRxiv - Immunology 2022
Quote:
... beads were incubated with varying amount of the recombinant his-tagged proteins and corresponding MFIs were compared to reference beads of know AlexFluor molecule densities (Bangs Laboratories, IN).
-
No products found
because this supplier's products are not listed.
Cassidy M.R. Blackburn, et al.,
bioRxiv - Immunology 2020
Quote:
... media was removed and replaced with either D-10 (untreated) or D-10 + 50μg/ml oxidized LDL (hi oxLDL Kalen biomedical 770252-60) for 2 hours ...
-
No products found
because this supplier's products are not listed.
Boyang Zhao, et al.,
bioRxiv - Biochemistry 2023
Quote:
... and σNS-ΔN17 were subcloned into the bacterial expression vector pET28 with an N-terminal His tag and a TEV protease cleavage site (Epoch Life Science). Escherichia coli DE3 cells (Novagen ...
-
No products found
because this supplier's products are not listed.
Erica Misner, Min Zhang, Eva Sapi,
bioRxiv - Microbiology 2022
Quote:
... PCR products were analyzed by standard agarose gel electrophoresis in a 1% gel referenced against a Hi-Lo DNA ladder (Bionexus, Inc., Oakland, CA, USA; BN2050). An infected zebrafish sample which was positive for B ...
-
No products found
because this supplier's products are not listed.
Camilla Kjeldgaard Larsen, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... The cleaved proteins were incubated with Ni-NTA beads for revers his-purification and cleaved proteins were collected in the flow through and visualized on SDS-PAGE (Mini Protean Tris-Tricine Precast gels, BioRad).