-
No products found
because this supplier's products are not listed.
James M. Burke, et al.,
bioRxiv - Immunology 2021
Quote:
... Mouse anti-Nuclear Pore Complex Proteins antibody (Abcam; ab24609) was used at 1:500 and detected with goat anti-mouse IgG H&L (FITC ...
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No products found
because this supplier's products are not listed.
Amy F Chen, et al.,
bioRxiv - Genomics 2021
Quote:
... nuclear pore complex antibody (Biolegend 902901), and GFP (Biolegend 338002) ...
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No products found
because this supplier's products are not listed.
Ruhul Amin, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... cells were harvested and nuclear protein complex lysates were prepared using the Nuclear Complex Co-IP Kit (Active Motif). Co-immunoprecipitation with two biological replicates was performed according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Reilly L. Allison, et al.,
bioRxiv - Neuroscience 2023
Quote:
... DAPI nuclear dye (Invitrogen) was used to label nuclei ...
-
No products found
because this supplier's products are not listed.
Andrea Sacchetti, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... The DAPI nuclear dye (D9542, Sigma-Aldrich) was used at 1 µg/ml to exclude dead cells ...
-
No products found
because this supplier's products are not listed.
Tanja Bhuiyan, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... the total protein concentration in the nuclear and cytoplasmic extracts was measured using Protein Assay Dye Reagent Concentrate (Biorad, 5000006). 1 mg nuclear or 3 mg cytoplasmic extract per pulldown was incubated in triplicates with 15 μl of a 50% slurry of GFP-trap beads (ChromoTek ...
-
No products found
because this supplier's products are not listed.
Axelle Brulport, et al.,
bioRxiv - Evolutionary Biology 2024
Quote:
... nuclei were resuspended in 500 μl of wash buffer and labelled with an anti-nuclear pore complex proteins antibody (clone Mab414) and sorted on a FACSAria instrument (BD Biosciences), with a 100 nozzle on a cold block ...
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No products found
because this supplier's products are not listed.
Xianhui Liu, et al.,
bioRxiv - Physiology 2024
Quote:
... Nuclear proteins were extracted using nuclear extraction buffer (NEB) (20mM HEPES pH7.9 ...
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No products found
because this supplier's products are not listed.
Ya-Juan Wang, et al.,
bioRxiv - Cell Biology 2022
Quote:
... The protein complex was isolated by immunoprecipitation using a mouse anti-His antibody (Cell Signaling, catalog #: 2366S) followed by SDS-PAGE and Western blot analysis with a rabbit anti-GABAA receptor α1 subunit antibody (R&D Systems ...
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No products found
because this supplier's products are not listed.
Aisha Hamid, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The complex was pulled down using Protein A or Protein G-sepharose beads (GE Healthcare) pre-equilibrated in lysis buffer for 2 h ...
-
No products found
because this supplier's products are not listed.
Amrita Rai, et al.,
bioRxiv - Biochemistry 2024
Quote:
... and Protein Complex suites (Qiagen). The sitting-drop vapor diffusion method was employed ...
-
No products found
because this supplier's products are not listed.
Florentina Negoita, et al.,
bioRxiv - Biochemistry 2024
Quote:
... The protein-antibody complex on the membranes were visualized by Odyssey XF Imager (LI-COR Biotech, LLC, Germany) after incubation of the membrane with the enhanced chemiluminescence substrate (Millipore ...
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No products found
because this supplier's products are not listed.
Jack H. Brelstaff, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Proteins were transferred onto 0.2 μm pore PVDF membranes (Merck). Nonspecific background was blocked in 5% w/v dry skimmed milk (Sigma ...
-
No products found
because this supplier's products are not listed.
Sk. Kayum Alam, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... and BioTracker nuclear red dye (Sartorius). The plates were placed in IncuCyte for up to 96 hours with images taken every 24 hours.
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No products found
because this supplier's products are not listed.
Junyan Lin, et al.,
bioRxiv - Cell Biology 2024
Quote:
... mouse monoclonal anti-Nup93 (E-8) (Santa Cruz Biotechnology, sc-374399), rabbit monoclonal anti-Nup98 (C39A3 ...
-
No products found
because this supplier's products are not listed.
N. Bishara Marzook, et al.,
bioRxiv - Cell Biology 2024
Quote:
... stained with nuclear dye Hoechst and imaged using a BioTek Cytation 5 (Agilent Technologies) to obtain total fluorescence intensities and area covered to obtain mean fluorescence intensities (MFI).
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No products found
because this supplier's products are not listed.
Victor Pikov, Douglas B. McCreery, Martin Han,
bioRxiv - Bioengineering 2020
Quote:
... Some sections were also immunostained with the antibody to neuron-specific nuclear protein NeuN (MAB377, 1:2K, Chemicon, Temecula, CA) and visualized using chromogen Vector Red (Vector Laboratories, Burlingame, CA). The stained sections were microphotographed using a 3-megapixel digital microscope camera (Spot RT ...
-
No products found
because this supplier's products are not listed.
Daniel Jun-Kit Hu, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Secondary antibodies were cyanine dyes from Jackson ImmunoResearch Laboratories (1:300) ...
-
No products found
because this supplier's products are not listed.
Goutam Dey, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... nuclear protein lysates were incubated with 3µg anti-Myc antibody (Proteintech) or 3µg control antibody (Cell Signaling Technology ...
-
No products found
because this supplier's products are not listed.
Dong Liu, et al.,
bioRxiv - Immunology 2024
Quote:
... and then incubated overnight at room temperature with fluorescently conjugated antibodies or DNA dyes (Nucspot ® Nuclear Stains 750/780, Biotium, #41038 ...
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No products found
because this supplier's products are not listed.
Mirca S. Saurty-Seerunghen, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Transwells (8μm pores, Corning) were coated for 30min with Matrigel (0.2mg/mL culture media ...
-
No products found
because this supplier's products are not listed.
Monika Rakoczy-Trojanowska, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... The protein-antibody complex was detected with the ECL Superbright system (Agrisera) and photographed using the ChemiDoc Imaging System (Bio-Rad ...
-
No products found
because this supplier's products are not listed.
Chenglong Xia, et al.,
bioRxiv - Biophysics 2024
Quote:
Nuclear-targeting Csm complex plasmid construction has been described previously12 (Addgene plasmid #195242). Cytoplasmic-targeting Csm complex plasmid was modified from this by removing the NLS sequences before each protein sequence ...
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No products found
because this supplier's products are not listed.
Anthony Chifor, Jeongyoon Choi, Joongkyu Park,
bioRxiv - Neuroscience 2021
Quote:
... The protein complex pulled down by GFP-Trap beads (Chromotek) was washed three times with RIPA buffer and eluted in 30 μl of 1x Laemmli sample buffers by heating at 75°C for 10 min.
-
No products found
because this supplier's products are not listed.
Seong Ho Hong, et al.,
bioRxiv - Biochemistry 2023
Quote:
... The SKP1 containing SCF complex was reconstituted by utilizing stoichiometric amount of the following recombinant proteins: Recombinant Human CUL1/RBX1 Neddylated Complex Protein (R&D systems, E3-411-025), Recombinant Human FBXO7 GST (N-Term ...
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No products found
because this supplier's products are not listed.
Rune Daucke, et al.,
bioRxiv - Bioengineering 2024
Quote:
... the antibody-antigen complex was visualized using Discovery Chromomap DAB-kit (Roche) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Henry J. Bailey, et al.,
bioRxiv - Biophysics 2024
Quote:
... CRBN protein was labelled with RED-Maleimide 2nd Generation dye (NanoTemper) optimized for degree-of-labeling of 1:1 molar ratio calculated by nanodrop ...
-
No products found
because this supplier's products are not listed.
Alexa R. Heaton, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Then a staining dye was added after secondary antibody washes: α-SMA – Opal-dye 520 (Akoya Biosciences OP-001001) at 1:100 ...
-
No products found
because this supplier's products are not listed.
Junghyun L. Suh, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... Cells were then segmented based upon the nuclear dye using the Harmony software (PerkinElmer). Propidium iodide intensity levels were calculated and cells were classified as ‘dead’ if their intensity was above the established threshold.
-
No products found
because this supplier's products are not listed.
D. Azimova, et al.,
bioRxiv - Microbiology 2021
Quote:
... We verified the identity of the proteins in the complex by western blot using native antibody for Cbp1 and a mouse anti-his tag antibody (ABclonal AE003) for Yps3-6xhis.
-
No products found
because this supplier's products are not listed.
Paul J. Myers, Sung Hyun Lee, Matthew J. Lazzara,
bioRxiv - Systems Biology 2023
Quote:
... Infrared dye-conjugated secondary antibodies were purchased from Rockland Immunochemicals (anti-mouse IgG DyLight 680 conjugated ...
-
No products found
because this supplier's products are not listed.
Takayuki Torisawa, et al.,
bioRxiv - Biophysics 2024
Quote:
Purified proteins or the artificial motor-cargo complex were diluted using 1× PBS (Takara Bio, T900) and loaded into custom-made microneedles prepared with the P1000IVF micropipette puller (Sutter Instrument) ...
-
No products found
because this supplier's products are not listed.
Nathanael A Caveney, et al.,
bioRxiv - Biochemistry 2023
Quote:
... The protein complex was eluted with the addition of 200 μg/mL of FLAG peptide (DYKDDDD) (GenScript). Protein was subsequently concentrated to >2 mg/mL and used for cryo-EM imaging.
-
No products found
because this supplier's products are not listed.
Yi Yu, et al.,
bioRxiv - Genomics 2020
Quote:
... Cas9 in base editor complex were quantified using anti-Cas9 antibody (7A9-A3A, Novus Biologicals). The protein concentration was adjusted to 0.1 nM (final concentration ...
-
No products found
because this supplier's products are not listed.
Thadeu Estevam Moreira Maramaldo Costa, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... Antibodies against human PCNA (proliferating cell nuclear antigen, rabbit) were purchased from Sino Biological (China). DNase I recombinant RNase free was purchased from Roche Life Sciences (Germany).
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No products found
because this supplier's products are not listed.
Yuting Wang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... 0.5 µL Tn5-Adapter complex (Diagenode) was added and incubated at 37°C for 30 min ...
-
No products found
because this supplier's products are not listed.
Stefan Niekamp, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... we labeled HALO-tagged49 proteins with HALO-Alexa488 or HALO-TMR dyes (Promega, G1001, G8251) by incubating the concentrated protein with dye for 1 hour on ice ...
-
No products found
because this supplier's products are not listed.
Emily S. Bell, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... infrared (IR)-dye conjugate secondary antibodies IRDye 800CW Donkey Anti-Goat (VWR, #102673-336), IRDye 800CW Donkey Anti-Rabbit (VWR ...
-
No products found
because this supplier's products are not listed.
Eloïne Bestion, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Cells were labeled with antibodies against viability fixable dye (Miltenyi Biotec, #130-109-812), CD3 (Miltenyi Biotec ...
-
No products found
because this supplier's products are not listed.
Ben Vanderkruk, et al.,
bioRxiv - Cell Biology 2022
Quote:
... and an equal mass of WT and KO nuclear lysate (∼30 μg protein) was immunoprecipitated by 1 μg WDR5 (Bethyl, A302-429A) or normal rabbit IgG (Millipore ...
-
No products found
because this supplier's products are not listed.
Hirotatsu Imai, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Antibodies against ribosomal proteins uL3 (GTX114725, GeneTex), L10a (A305-061A ...
-
No products found
because this supplier's products are not listed.
Michael Ferns, et al.,
bioRxiv - Neuroscience 2024
Quote:
... A dye-conjugated light chain-specific anti-rabbit secondary antibody (Jackson Labs) was used to detect bound Kv5.1 antibody ...
-
No products found
because this supplier's products are not listed.
Dingding Mo,
bioRxiv - Neuroscience 2022
Quote:
... About 40 µg total protein of the supernatants were fractioned on 8–20% precast protein gels and transferred to 0.2 µm pore-size nitrocellulose membranes (66485, Pall). For monomer Aβ detection ...
-
No products found
because this supplier's products are not listed.
Katharina Kases, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... antibodies in Protein LoBind tubes (Eppendorf). Thereafter ...
-
No products found
because this supplier's products are not listed.
Jeeyeon Cha, et al.,
bioRxiv - Cell Biology 2022
Quote:
... DAPI was used for nuclear staining (Southern Biotech).
-
No products found
because this supplier's products are not listed.
Zaki Al-Yafeai, et al.,
bioRxiv - Cell Biology 2020
Quote:
... NF-κB nuclear translocation was visualized by Leica TCS SP5 confocal microscope using 63X (oil objective ...
-
No products found
because this supplier's products are not listed.
Arun Chhikara, et al.,
bioRxiv - Neuroscience 2024
Quote:
Primary antibodies and dyes used in this study were purchased from the following sources: Bassoon (Synaptic Systems, 141 004, 141021), Beta-actin (Synaptic Systems ...
-
No products found
because this supplier's products are not listed.
Xinyi Zhang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... but with different rabbit polyclonal antibodies from the Human Protein Atlas (HPA) purchased from Atlas Antibodies. On the next day ...
-
No products found
because this supplier's products are not listed.
Rebekah L. Rashford, et al.,
bioRxiv - Neuroscience 2024
Quote:
... (3) Incubating the slices in primary antibody [1:500 Anti-Green Fluorescent Protein (Chicken) Antibody (Aves Labs); 1:150 SETD7 Mouse Monoclonal Antibody (OriGene) ...
-
No products found
because this supplier's products are not listed.
James T. Van Leuven, et al.,
bioRxiv - Microbiology 2021
Quote:
... Antibodies specific for PR8 hemagglutinin protein (NR-3148, BEI Resources) and Ly6G (1A8, Bio X Cell) were detected with immPACT vector red and immPACT DAB ...