Laura Ohl, et al.,bioRxiv - Molecular Biology 2023
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... Tissue was mixed with 1 mL 40:40:20 methanol:acetonitrile:water (extraction solvent) and homogenized with a benchtop lyser (SciLogex OS20-S) at 1600 mhZ for approximately 15 sec ...
Kevin M. Tharp, et al.,bioRxiv - Cell Biology 2020
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... 1 μL of sample was separated over a 25 cm column packed with 1.9 μm Reprosil C18 particles (Dr. Maisch HPLC GmbH) by a nanoElute HPLC (Bruker) ...
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Human immature dendritic cells (imDCs) were obtained from peripheral blood mononuclear cells (PBMC) from HIV-1 seronegative donors using a Ficoll-Hypaque gradient (Alere Technologies AS). The monocyte population was selected by adherence on a T75cm2 flask for 1 hour ...
Ezekiel C. Thomas, Amber Ismael, Jeffrey K. Moore,bioRxiv - Cell Biology 2020
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... and 1% yeast extract) at 30°C then diluted into synthetic media (2% glucose, CSM from Sunrise Science Products, #1001 San Francisco, CA) and grown to log phase at 30°C ...
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... Blood samples were collected at the inlet and outlet for measuring blood cell count (2 mL in K2EDTA) and aPTT/PT (3 mL in 1:9 citrate) using a clinical hematology analyzer (Diagnostica Stago Start 4, Siemens, Germany).12 If aPTT was outside the range of 20-50 seconds ...