Arundhasa Chandrabalan, et al.,bioRxiv - Pharmacology and Toxicology 2020
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... All joint fluids were tested for presence of any blood with the Fecal Occult Blood Test kit (Immunostics Hema-Screen) and the samples showing presence of blood were excluded from further analysis.
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... Immunocomplexes were visualized using the ECL substrates kits from Cyanagen and imaged on ALLIANCE MINI HD9 system (UVITEC Ltd, UK). Quantification of bands was performed using the Uvitec Nine Alliance Software ...
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... and correctly assembled clones were midi-prepped using Qiagen kits and integrated into the genome at the attP2 third-chromosome site by BestGene, Inc (Chino Hills ...
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... 20μL of media is used to measure luciferase activity using Targeting Systems Cypridinia and Targeting systems Gaussia luciferase assay kits (Targeting Systems) on a Biotek Synergy 4 plate reader with an injection protocol ...
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... 15 μl of concentrated and filtered ACM (500 μl from 10 mL/sample) or calibration particles included in the reagent kit were placed in the Nanopore (NP150, Izon Science). Samples were measured at 44∼45 mm stretch with a voltage of 0.6∼0.8 V at 1-pressure levels of 10 mbar ...
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... The suspension was then transferred to a filter cartridge provided by the kit in a 2 mL tube (CELLTREAT, catalog number: 229446), followed by centrifuging at 400 g for 2 min and 800 g for 4 min ...