-
No products found
because this supplier's products are not listed.
Doowon Huh, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Lysate was digested with RNase I (Lucigen) before ribosomes were isolated through ultracentrifugation through a sucrose cushion ...
-
No products found
because this supplier's products are not listed.
Joshua J. Sims, et al.,
bioRxiv - Genetics 2021
Quote:
... We measured reporter virus transduction activity on a luminometer (BioTek) using the Renilla Glo Kit (Promega ...
-
No products found
because this supplier's products are not listed.
Kuei-Chen Wang, et al.,
bioRxiv - Immunology 2023
Quote:
... or heart tissue lysate (Novus Biologicals) was resolved on 4-20% gradient SDS-PAGE (Bio-Rad ...
-
No products found
because this supplier's products are not listed.
Patrick Jouandin, et al.,
bioRxiv - Genetics 2019
Quote:
... Lysates were resolved by electrophoresis (Mini-PROTEAN TGX Precast Gels ...
-
No products found
because this supplier's products are not listed.
Heesun Kim, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Worm lysates were generated with FastPrep (MP Biomedicals) at a speed of 6 m/s for 20 s for four times ...
-
No products found
because this supplier's products are not listed.
Prashant Rajbhandari, et al.,
bioRxiv - Cell Biology 2019
Quote:
Whole cell lysate or tissue lysate was extracted using RIPA lysis buffer (Boston Bioproducts) supplemented with complete protease inhibitor cocktail (Roche) ...
-
No products found
because this supplier's products are not listed.
Robbert Boudewijns, et al.,
bioRxiv - Microbiology 2020
Quote:
... virus stocks were confirmed to be free of mycoplasma (PlasmoTest, InvivoGen) and other adventitious agents by deep sequencing on a MiSeq platform (Illumina ...
-
No products found
because this supplier's products are not listed.
Joseph M Gibbons, et al.,
bioRxiv - Microbiology 2019
Quote:
... Virus-like particles (VLPs) were produced by linear polyethylenimine 25K (Polysciences) transfection of HEK-293T ...
-
No products found
because this supplier's products are not listed.
Jenny Y. Pham, et al.,
bioRxiv - Evolutionary Biology 2019
Quote:
This study used Escherichia virus T4 (American Type Culture Collection [ATCC] #11303-B4) and three wild-type bacterial hosts ...
-
No products found
because this supplier's products are not listed.
Heejoo Kim, et al.,
bioRxiv - Immunology 2020
Quote:
... Lysates were incubated with 2.5 μg Jmjd1A antibody (Bethyl) and protein-G Dynabeads (ThermoFisher ...
-
No products found
Svenja Fritzlar, et al.,
bioRxiv - Microbiology 2023
Quote:
... virus was activated by adding 8μg/mL trypsin (TPCK treated, Worthington, Lakewood, NJ) in FCS-free media to samples in a 1:1 ratio ...
-
No products found
because this supplier's products are not listed.
Andrii Slonchak, et al.,
bioRxiv - Microbiology 2021
Quote:
... Virus replication foci were counted using the Image Studio Lite software (v5.2.5, LI-COR, USA), and titres were determined based on dilution factors and expressed as focus forming units per mL (FFU mL^−1).
-
No products found
Yang Li, et al.,
bioRxiv - Immunology 2021
Quote:
PAOC cells were immortalized with Lenti-hTERT virus (ABM; cat# G200) following manufacturer’s instructions and clonal sorting/expansion ...
-
No products found
because this supplier's products are not listed.
Larise Oberholster, et al.,
bioRxiv - Neuroscience 2023
Quote:
... consisting of 1 ug/ml of JC virus BIO-PROBE® (Enzo Life Sciences) in 1x In Situ Hybridization buffer (Enzo Life Sciences) ...
-
No products found
because this supplier's products are not listed.
Timothy P. Sheahan, et al.,
bioRxiv - Microbiology 2020
Quote:
... nanoluciferase expression as a surrogate for virus replication was quantitated on a Spectramax (Molecular Devices) plate reader according to the manufacturer’s instructions (Promega ...
-
No products found
because this supplier's products are not listed.
Yilin Wang, et al.,
bioRxiv - Immunology 2021
Quote:
... cell lysate (125 μg) was added to 5 μg GGA3-PBD beads (Cytoskeleton, USA) to pull down ARFs-GTP and incubated at 4°C on a rotator for 1h and then the beads were extensively washed to remove unbound protein ...
-
No products found
because this supplier's products are not listed.
Yoshiaki Nishimura, et al.,
bioRxiv - Microbiology 2020
Quote:
... Cells were grown to ~90% confluency in a 96-well plate prior to addition of serially diluted virus to achieve luminescence signals of 30,000-1,000,000 (BMG Labtech PolarStar Optima plate reader at maximum gain) ...
-
No products found
because this supplier's products are not listed.
Bei Zhao, Yinghua Liu, Xuemei Tang, Shi Cheng,
bioRxiv - Cancer Biology 2022
Quote:
... RIPA lysate (Solarbio, Beijing, China), N,N′-methylenebisacrylamide (Aladdin ...
-
No products found
because this supplier's products are not listed.
Enakshi Roy, Wen Shi, Bin Duan, St Patrick Reid,
bioRxiv - Microbiology 2020
Quote:
... Mock or virus-infected cells were fixed in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, USA) for 30 min and permeabilized with 0.1% Triton X (Fisher Bioreagents ...
-
No products found
because this supplier's products are not listed.
Qiulong Yan, et al.,
bioRxiv - Microbiology 2020
Quote:
The DNA and RNA of virus were extracted by using TIANamp Virus DNA / RNA Kit (TIANGEN) according to the manufacturer’s protocols ...
-
No products found
because this supplier's products are not listed.
Xiaonan Zhang, et al.,
bioRxiv - Microbiology 2020
Quote:
... The blood samples from enrolled patients were tested for hepatitis B virus surface antigen (HBsAg) and hepatitis B virus e antigen (HBeAg) by Abbott AXSYM HBsAg (normal ...
-
No products found
because this supplier's products are not listed.
Kyoko Tossell, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Stereotaxic virus injections were performed using an Angle Two apparatus (Leica) linked to a digital brain atlas (Leica Biosystems Richmond ...
-
No products found
because this supplier's products are not listed.
Qixiang He, et al.,
bioRxiv - Biochemistry 2021
Quote:
... The virus titers were measured by flow cytometry assay (Expression Systems).
-
No products found
because this supplier's products are not listed.
Justin J. Botterill, et al.,
bioRxiv - Neuroscience 2021
Quote:
Virus was delivered using a 500nL Neuros Syringe (#65457-02, Hamilton Company) attached to the stereotaxic apparatus with a probe holder (#751873 ...
-
No products found
because this supplier's products are not listed.
CP Profaci, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Vessel preparations from mice on PLX5622 diet or expressing LDLR virus were treated with bath application of 5mM methyl-beta cyclodextrin (MβCD) (Cayman Chemicals 21633) for 30 minutes to deplete membrane cholesterol.
-
No products found
because this supplier's products are not listed.
Jian Hang Lam, et al.,
bioRxiv - Immunology 2022
Quote:
... with Trans IT-Virus GEN (Mirus). In brief ...
-
No products found
because this supplier's products are not listed.
Pengxiang Chang, et al.,
bioRxiv - Microbiology 2022
Quote:
... Virus was diluted in HBS-EP buffer (TEKnova) containing 10 μM oseltamivir carboxylate (Roche ...
-
No products found
because this supplier's products are not listed.
Emily A. Hemann, et al.,
bioRxiv - Immunology 2022
Quote:
... this virus/serum mixture is incubated with 0.5% chicken red blood cells (Rockland) and hemagglutination inhibition was measured after 30 minutes.
-
No products found
because this supplier's products are not listed.
Maria Kuzikov, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... plates were transported to the CAPS-IT robotic system for the addition of virus dilution (MOI = 0.001) using a liquid handler (EVO 100, Tecan) to a final well volume of 60 μl ...
-
No products found
because this supplier's products are not listed.
Teodors Pantelejevs, Marko Hyvönen,
bioRxiv - Biochemistry 2022
Quote:
... lysate was loaded on a 3 mL Ni-NTA agarose matrix (Cube Biotech), after which column matrix was washed with 10 CV Nickel Buffer A ...
-
No products found
because this supplier's products are not listed.
Joydeb Sinha, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Harvested virus was 0.4μM filtered (Celltreat 229749) and concentrated by centrifugation using a 100kdA cut-off PES protein concentrator (Pierce 88533 ...
-
No products found
because this supplier's products are not listed.
Paul K. LaFosse, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Virus was injected unilaterally with a stereotactic syringe pump (Stoelting; Wood Dale, IL) through a pulled glass pipette tip cut to an opening of 10–15 μm diameter ...
-
No products found
because this supplier's products are not listed.
Helene Sertznig, et al.,
bioRxiv - Microbiology 2021
Quote:
... The virus containing supernatant was then purified by filtration through 0.30 μm MACS SmartStrainers (Miltenyi Biotec), aliquoted and stored at -80 °C ...
-
No products found
because this supplier's products are not listed.
Lauren Rodriguez, et al.,
bioRxiv - Microbiology 2019
Quote:
... The lysate was subjected to bead beating (Mini-Beadbeater, Biospec Products) followed by a chloroform extraction ...
-
No products found
because this supplier's products are not listed.
Michael Bauer, et al.,
bioRxiv - Microbiology 2020
Quote:
... Genome-labeled AdV was produced by growing the virus in A549 cells in the presence of 2.5 μM EdC (5-ethynyl-2’-deoxycytidine, Jena Biosciences) as described 52 ...
-
No products found
because this supplier's products are not listed.
Xiaofeng Zheng, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Insulin and protein content of the lysate were measured using Mouse Insulin ELISA (Mercodia) and BCA assay (ThermoFisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Ferdinand Roesch, et al.,
bioRxiv - Microbiology 2022
Quote:
... Virus was diluted in RPMI-1640 (Genesee Scientific) media containing 2% FBS and 10 mM HEPES ...
-
No products found
because this supplier's products are not listed.
Stuti Mehta, et al.,
bioRxiv - Molecular Biology 2022
Quote:
AAVPrime™ Adeno-associated virus (AAV) Serotype Testing Kit (GeneCopoeia) was used to determine the efficiency of 8 different AAV serotypes in infecting the HCC1187 cell line ...
-
No products found
because this supplier's products are not listed.
M. Bormann, et al.,
bioRxiv - Microbiology 2021
Quote:
... the infectious virus was recovered by vortexing the carriers placed in plastic containers (SARSTEDT, Nümbrecht, Germany) filled with 2 mL DMEM for 1 minute ...
-
No products found
because this supplier's products are not listed.
Guus Franken, et al.,
bioRxiv - Immunology 2023
Quote:
... Serially diluted lysates were arrayed on nitrocellulose-coated slides (Grace Bio-Labs) by the Quanterix (Aushon ...
-
No products found
because this supplier's products are not listed.
Kevin J. Kramer, et al.,
bioRxiv - Immunology 2021
Quote:
... values at the endpoint (48 h after incubation with the virus) were determined using the RTCA software version 2.1.0 (ACEA Biosciences Inc.). Results are expressed as percent neutralization in a presence of respective antibody relative to control wells with no CPE minus CI values from control wells with maximum CPE ...
-
No products found
because this supplier's products are not listed.
Lucy Chou-Zheng, Asma Hatoum-Aslan,
bioRxiv - Microbiology 2022
Quote:
... Cleared lysates were passed through a 5 ml gravity column (G-Biosciences, MO, USA) containing 1.5 ml of Ni2+-NTA agarose resin (ThermoFisher Scientific ...
-
Native Antigen
Cat# NAT41572-100,
100ug USD $426.0
Ask
Subhasis Mahari, et al.,
bioRxiv - Bioengineering 2020
Quote:
... Human Immunodeficiency Virus (HIV) and Avian Influenza Virus (AIV) Ag were obtained from The Native Antigen Company (Oxford, UK). Aurdino software has been used in the in-house built device and the hardware include a printed Circuit Board (PCB) ...
-
No products found
because this supplier's products are not listed.
Qingtao Sun, et al.,
bioRxiv - Neuroscience 2023
Quote:
... IL-6 levels were quantified in the lysates using a Mouse IL-6 ELISA specific for lysates (#ELM-IL6-CL-1; RayBiotech).
-
No products found
because this supplier's products are not listed.
José-María Díez, Carolina Romero, Rodrigo Gajardo,
bioRxiv - Immunology 2020
Quote:
... Human Anti-SARS-CoV-2 Virus Spike 1 [S1] IgG ELISA Kit (Alpha Diagnostic Intl. Inc.), against S1 subunit spike protein ...
-
No products found
because this supplier's products are not listed.
Matthew T. Birnie, et al.,
bioRxiv - Neuroscience 2022
Quote:
Virus labeled sections were scanned under a 10x objective of a fluorescent microscope (Olympus BX 61) equipped with a high-sensitivity CCD camera (Nikon Eclipse E400) ...
-
No products found
because this supplier's products are not listed.
Wren E. Michaels, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Cell lysates were prepared using the sample preparation kit (Protein Simple) for an automated capillary western blot system ...
-
No products found
because this supplier's products are not listed.
Christopher M. Schafer, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Incubation of cell lysates with polyubiquitin-affinity beads (Calbiochem: #662200-1KIT) was carried out according to the manufacturer’s protocol followed by immunoblotting with an anti-ERG Ab (Santa Cruz ...
-
No products found
because this supplier's products are not listed.
JF Sturgill, et al.,
bioRxiv - Neuroscience 2020
Quote:
... AAV virus (300nL volume) was then pressure injected 100nL/min via a glass pipette pulled (P-97 Sutter Instruments) from borosilicate capillaries (Drummond calibrated 5ul ...
-
No products found
because this supplier's products are not listed.
Lakshmi E. Batachari, et al.,
bioRxiv - Immunology 2024
Quote:
... samples were incubated with Quasar 670-conjugated FISH probes that hybridize to Orsay virus RNA1 and RNA2 (Biosearch Technologies). Following a 6-8 h incubation at 47 °C ...