Quote:
Calpain-2 immunostaining was performed using a rabbit anti-human calpain-2 (10 µg/ml, catalog No. RP-3 Calpain-2; Triple Point biologics, Forest Grove, OR). CD68 immunostaining was performed using a rabbit anti-human CD68 antibody (1:200 ...
Marissa Saenz, et al.,bioRxiv - Pharmacology and Toxicology 2022
Quote:
... Following precipitation of plasma proteins with acetonitrile containing 3.5 ng/mL buprenorphine-d4 (Cerilliant, Round Rock, TX, USA) as an internal standard ...
Paulus G.M. Jochems, et al.,bioRxiv - Bioengineering 2020
Quote:
... Protein content in the digests as assessed in triplicate on a Flash EA 1112 GC (Interscience BV, Breda, The Netherlands) according the Dumas method (45) ...
Salvatore Rizza, et al.,bioRxiv - Cancer Biology 2019
Quote:
... incubated for 1 h in blocking solution and then over night with an anti-Microtubule-associated proteins 1A/1B light chain 3B (anti-LC3, 0231-100, Nanotools) and anti-TOM20 (Santa Cruz Biotechnology ...
Adam R. Bentham, et al.,bioRxiv - Plant Biology 2023
Quote:
... SDS-PAGE/immunoblot analysis was used to identify proteins in the sample with use of anti-GFP antibody (Cohesion Biosciences) and anti-mRFP antibody (Abcam ...
Charlotta Hansson, et al.,bioRxiv - Immunology 2023
Quote:
EAE was induced in 8-9 weeks old C57BL/6 mice or SJL/J mice after an intradermal injection with 100µg myelin oligodendrocyte glycoprotein (MOG35-55) or proteolipid protein (PLP139-151) peptide (MD Biosciences) emulsified in Complete Freund’s Adjuvant (CFA ...
Quote:
... we determined the concentration of the positive acute phase protein Haptoglobin (Hp; Phase Haptoglobin Assay TP-801, Tridelta Development Limited, Maynooth, Ireland). All biomarkers related to nutrient utilization and inflammation were determined using a small-scale biochemistry analyzer (CataChemWell-T ...
Dmytro Morderer, et al.,bioRxiv - Neuroscience 2024
Quote:
... Immunostained sections were washed 3×5 minutes with Wash Buffer followed by HRP-mediated protein proximity labelling in Labelling Buffer (1xTBS, 5 uM biotin-ss-tyramide (Iris Biotech, LS-3570), 0.03% H2O2 ...
Lauren G. Mascibroda, et al.,bioRxiv - Biochemistry 2020
Quote:
... pTEF or pTEFdual plasmids were included to express proteins in trans and were transformed with pOBD plasmids and selected on media lacking tryptophan and uracil (Sunrise Science Products, San Diego CA, 1316-030). Yeast strains were then mated and subsequently selected on medium lacking tryptophan ...