-
No products found
because this supplier's products are not listed.
Athina Varveri, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... p62 MBL (rabbit; 1:500; MBL) and LC3 (mouse; 1:20; NanoTools) (in PSI Buffer ...
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No products found
because this supplier's products are not listed.
John N. Koberstein, et al.,
bioRxiv - Biochemistry 2020
Quote:
... coli MBP (malE) was obtained as a gBlock (IDT DNA, Table S1). BsaI sites were added to ends by PCR with complementary overhangs for golden gate cloning into pATT-Dest (Addgene plasmid #79770 ...
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No products found
because this supplier's products are not listed.
Doris Krauter, et al.,
bioRxiv - Neuroscience 2021
Quote:
... using a diamond knife (Histo HI 4317, Diatome). Afterwards sections were stained according to Gallyas52 and with Methylene blue/ Azur II for 1 min ...
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Cystatin-C ELISA / assay Kit
Cat# K012-H1,
1.0 ea, USD $385.0
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Lisa Mohr, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... 4°C for 10 min and 2′3′-cGAMP levels were quantified using the 2′3′-cGAMP ELISA Kit (Arbor Assays) according to the manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Naomi-Liza Denning, et al.,
bioRxiv - Immunology 2019
Quote:
... Macrophages from healthy human donors were obtained from Hemacare (Cat. No. PBMACC-MON-2, Northridge, CA). Murine peritoneal macrophages were isolated from adult male mice38 ...
-
No products found
because this supplier's products are not listed.
Dennis J Doorduijn, et al.,
bioRxiv - Immunology 2019
Quote:
... Nunc Maxisorp ELISA plates were coated overnight at 4 °C with 50 µl/well of 3 µg/ml monoclonal mouse IgG1 anti human C6 (Quidel) in PBS ...
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No products found
because this supplier's products are not listed.
Martin H. Berryer, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Starting on day 2 human induced neural progenitor-like cells were harvested with Accutase (Innovative Cell Technology, Inc., AT104-500) and re-plated at 15,000 cells.cm−2 in Astrocyte Medium (ScienCell ...
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No products found
because this supplier's products are not listed.
Rachel M. McLaughlin, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Rat cortices were cut into small pieces and digested for 30 minutes at 30°C in 2 mg/mL papain dissolved in Hibernate A without Calcium (BrainBits). After removal of papain ...
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No products found
because this supplier's products are not listed.
Bahia Bekhouche, et al.,
bioRxiv - Genetics 2019
Quote:
... incubated for 2 hours at 37 †C in IM with 200 μCi/ml3H palmitic acid (9,10-3H(N)) (American Radiolabeled Chemicals, Inc.). The cells were washed ...
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No products found
because this supplier's products are not listed.
Bibiana Rius, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Lysates containing 50 μg of protein were then incubated for 2 h with shaking at 37°C with 100 μM TAMRA azide (Click Chemistry Tools Scottsdale, Az), 800 μM copper(II ...
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No products found
because this supplier's products are not listed.
Asha A. Philip, John T. Patton,
bioRxiv - Microbiology 2022
Quote:
... A puc19 plasmid containing a RIX/NSP3-2A-P-His insert under the control of a T7 transcription promoter (puc19/T7/ RIX/NSP3-2A-P-His) was purchased from Bio Basic Canada Inc ...
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No products found
because this supplier's products are not listed.
Oskar Staufer, et al.,
bioRxiv - Immunology 2022
Quote:
... beads were incubated with varying amount of the recombinant his-tagged proteins and corresponding MFIs were compared to reference beads of know AlexFluor molecule densities (Bangs Laboratories, IN).
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No products found
because this supplier's products are not listed.
Vanessa Nunes, et al.,
bioRxiv - Cell Biology 2019
Quote:
... 5]-PEG(2) (SuSoS) in 10 mM HEPES at pH 7.4 ...
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No products found
because this supplier's products are not listed.
Caro-Astorga Joaquin, et al.,
bioRxiv - Microbiology 2019
Quote:
... reduced with 2 μL of 50 mM Tris(2-carboxyethyl) phosphine (TCEP, SCIEX), pH 8.0 ...
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No products found
because this supplier's products are not listed.
Etai Sapoznik, et al.,
bioRxiv - Biophysics 2020
Quote:
... #1.5 coverslips (0420-0323-2, Bioptechs) were washed at room temperature in solution consisting of 1:1 (vol/vol ...
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No products found
because this supplier's products are not listed.
Shahrnaz Kemal, et al.,
bioRxiv - Neuroscience 2023
Quote:
... All vectors contain an N-terminal 6x-His-tag, C terminal Strep-Tag II tag, with or without a N or C terminal fluorophore (EGFP, mNeonGreen (Allele Biotech), or mRuby2).
-
No products found
because this supplier's products are not listed.
Shuang Fu, et al.,
bioRxiv - Biophysics 2022
Quote:
... Ara-C (2 mM, TargetMol, T1272) was added to the medium to inhibit neural stem cell proliferation ...
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No products found
because this supplier's products are not listed.
Myung Chung, et al.,
bioRxiv - Neuroscience 2024
Quote:
... HEK293-EGFP (GenTarget, Cat. No. #SC001), and NIH-3T3 (originally obtained from Riken Bioresource Research Center and gifted from Dr ...
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No products found
because this supplier's products are not listed.
Luis Alfonso Yañez Guerra, Meet Zandawala,
bioRxiv - Evolutionary Biology 2023
Quote:
... HEK293-G5a (Angio-proteomie CAT no. cAP0200GFP-AEQ-Cyto) cells were cultured in 96 well-plates containing 100μl of DMEM (Thermo ...
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No products found
because this supplier's products are not listed.
Amanda J. Stock, et al.,
bioRxiv - Immunology 2024
Quote:
... and 2-mercaptoethanol) at 37°C in a Jitterbug Microplate Shaker (Boekel Scientific). After 30 minutes (min ...
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No products found
because this supplier's products are not listed.
Michael P. Doyle, et al.,
bioRxiv - Immunology 2022
Quote:
... his-tagged YFV E protein (Meridian Life Science) was associated to the tips at 5 μg/mL for 60 sec ...
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No products found
because this supplier's products are not listed.
Natali Ozber, et al.,
bioRxiv - Plant Biology 2022
Quote:
The phospholipid-binding analysis of the MBP-MP was performed using PIP Strips (P-6001; Echelon Biosciences). The PIP strips were blocked for 1 h at room temperature in Tris-buffered saline (TBS ...
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No products found
because this supplier's products are not listed.
Saejeong Park, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... HEK293 cells were washed with PBS and lysed in NP-40 lysis buffer (Boston Bioproducts) including protease and phosphatase inhibitor (Roche) ...
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WB, IF, IHC,ELISA
Cat# A5006, SKU# A5006-100ul,
100ul, $157.00
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Xuxiao He, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... and Nickel Magnetic Beads for His tag protein purification (Bimake, B23602) according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Mu-Sen Liu, et al.,
bioRxiv - Biochemistry 2019
Quote:
... The His6-MBP-Cas9 fusion protein was cleaved to remove the His6-MBP tag by adding the TEV protease to the dialysis tubing (Spectrum labs) during dialysis against buffer containing 20 mM HEPES pH 7.5 ...
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No products found
because this supplier's products are not listed.
Deborah C. Mendonça, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Micrographs of SEPT5-SEPT6-SEPT7 complexes (with or without MBP) were recorded using an F416 camera (TVIPS, Germany) with a JEM-2100 (JEOL, Japan) microscope operated at 200 kV with a pixel size of 1.78 Å ...
-
No products found
because this supplier's products are not listed.
Rebecca J. Haugen, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 3 µL of 5 µM Click-Adapter (5’ Hexynyl-12(N)AGATCGGaaGAGCGTCGTGTAGGGaaAGAGTGTAGATCTCGGTGGTCGCCGTATCATT) and 0.4 µL premixed 50 mM vitamin C and 2 µL of 10mM Cu-TBTA (Lumiprobe). The alkyne-azide cycloaddition of the adapter was catalyzed twice ...
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No products found
because this supplier's products are not listed.
TM Tessi, et al.,
bioRxiv - Plant Biology 2020
Quote:
... 5 mM EDTA before being lysed at 2°C with an automatic cell pressure lysis machine at 0.3 KBar (Constant Systems) in homogenization buffer (50 mM Tris adjusted to pH 8.0 with MES ...
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No products found
because this supplier's products are not listed.
Meghal Desai, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Lysates clarified by centrifugation at 10,000g for 15 minutes at 4°C were incubated with 5ul of serum containing antibodies for 2 hours at 4°C followed by the addition of pre-blocked (with 1% BSA) Protein-A beads (Repligen) for an additional 2 hours ...
-
No products found
because this supplier's products are not listed.
Lanpeng Chen, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... anti-human Nanog and anti-human Oct-4 were obtained from Cell Science Signaling ...
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No products found
because this supplier's products are not listed.
E.A. Matthews, et al.,
bioRxiv - Neuroscience 2021
Quote:
... in a temperature (22±2°C) and humidity (55±10%) controlled environment with food/water ad libitum and nesting material (nestlets, Ancare, USA). Animals were allowed at least 1 week of acclimatization to the animal facility before surgery and singly housed after surgery ...
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No products found
because this supplier's products are not listed.
Dhruva D. Dhavale, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The mix was incubated for 2 h at 37 °C with shaking (1000 rpm) in an Incu-Mixer MP2 (Catalog H6002 Benchmark Scientific). Samples were transferred to Multiscreen FB Filter plates (Catalog MSFBN6B50 ...
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No products found
because this supplier's products are not listed.
Peter Koppensteiner, et al.,
bioRxiv - Neuroscience 2022
Quote:
... in 2% BSA in TBS at 4 °C for 2 O/N and respective 1.4 nm gold-conjugated secondary antibodies (Nanoprobes Inc., USA) for silver intensification or in biotinylated anti-rabbit secondary antibody (Vector Labs ...
-
No products found
because this supplier's products are not listed.
Anne Rehkamp, et al.,
bioRxiv - Biochemistry 2020
Quote:
LC separation of peptides originating from HEK293 cell membranes was performed via selfpacked C18 columns (PicoFrits, 75 μm x 50 cm, 15 μm tip, New Objective, self-packed with ReproSil-Pur 120 C18-AQ ...
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No products found
because this supplier's products are not listed.
Balaji Karthick Subramanian, et al.,
bioRxiv - Pathology 2019
Quote:
... Human primary podocytes from Celprogen Inc ...
-
No products found
because this supplier's products are not listed.
Sylwia Machcinska, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... and C (CELLnTEC, Switzerland) was added per insert ...
-
No products found
because this supplier's products are not listed.
Manuel Göpferich, et al.,
bioRxiv - Neuroscience 2020
Quote:
... human FGF (20 ng/μl, ReliaTech) and human EGF (Promokine) ...
-
No products found
because this supplier's products are not listed.
Lior Tal, et al.,
bioRxiv - Plant Biology 2023
Quote:
... Arabidopsis KAI2 protein was expressed as a 6× His-SUMO fusion protein using the expression vector pSUMO (LifeSensors). His-SUMO-KAI2 was isolated from by Ni-NTA resin and the eluted His-SUMO KAI2 was further separated by anion-exchange ...
-
No products found
because this supplier's products are not listed.
Tobias Tertel, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... 12 nM anti-human CD63 PE (EXBIO) or 13 nM anti-human CD81 PE (Beckman Coulter) ...
-
No products found
because this supplier's products are not listed.
Alicja Przybyszewska-Podstawka, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... TGFβ (human TGFβ1, Biorbyt, Cambridge, United Kingdom), Gibson Assembly® Master Mix (NEB) ...
-
No products found
because this supplier's products are not listed.
Inci Ramírez-Bello, et al.,
bioRxiv - Microbiology 2024
Quote:
... A secondary anti-human IgG coupled to peroxidase (Aviva SB) was added at a dilution of 1:1,000 in PBS.
-
No products found
because this supplier's products are not listed.
R. Wercberger, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 2% Fluoro-Gold (Fluorochrome), or the HSV-hEF1a-GFP-L10a.
-
No products found
because this supplier's products are not listed.
Lisa-Marie Appel, et al.,
bioRxiv - Biochemistry 2022
Quote:
Crystalization was performed at at 22°C or 4°C using a sitting-drop vapour diffusion technique and micro-dispensing liquid handling robot Mosquito (TTP labtech). The best diffracting crystals of SHARP SPOC:1xS5P CTD were grown at 22°C in conditions E9 from ShotGun HT screen (SG1 HT96 Molecular Dimensions ...
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No products found
because this supplier's products are not listed.
Amy L. Kimble, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Frozen mouse and human brain tissues were mechanically homogenized (Next Advance Bullet Blender BB724M with 3.2 mm stainless steel beads using setting 4 for 4min at 4C) ...
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No products found
because this supplier's products are not listed.
Camilla Kjeldgaard Larsen, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... The cleaved proteins were incubated with Ni-NTA beads for revers his-purification and cleaved proteins were collected in the flow through and visualized on SDS-PAGE (Mini Protean Tris-Tricine Precast gels, BioRad).
-
No products found
because this supplier's products are not listed.
Divyesh Joshi, et al.,
bioRxiv - Cell Biology 2024
Quote:
... Deidentified human specimens were deparaffinized in Histo-Clear (National Diagnostics, HS-200). Sections were progressively rehydrated before antigen retrieval for 30 min at 95°C in 1X Antigen Retrieval Buffer (Dako ...
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No products found
because this supplier's products are not listed.
Yusuke Nasu, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... by a Nanoject 2 (Drummond Scientific). Five ...
-
No products found
because this supplier's products are not listed.
Carole Lartigue, et al.,
bioRxiv - Microbiology 2021
Quote:
... They were observed at −178 °C using a cryo holder (626, Gatan), with a ThermoFisher FEI Tecnai F20 electron microscope operating at 200 kV under low-dose conditions ...
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No products found
because this supplier's products are not listed.
Yosif Zaki, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Layers of adhesive cement (C&B Metabond) followed by dental cement (A-M Systems) were spread over the surgical site ...
-
No products found
because this supplier's products are not listed.
Mastura Akter, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Stainless steel guide cannulae (Double/O.D.0.41mm-27G/C, cat # 62069, RWD life science.com.ltd.) were bilaterally positioned into ACC region (2.4 mm anterior to bregma and 0.5 mm lateral from midline ...