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No products found
because this supplier's products are not listed.
Amine Driouchi, et al.,
bioRxiv - Biophysics 2023
Quote:
... ribonucleoprotein (RNP) complexes included sgRNAs targeting either N- or C-terminal region (Integrated DNA Technologies - IDT) and Cas9 protein (PNA bio, Cat #CP01) were transfected together with linear dsDNA donors (IDT ...
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No products found
because this supplier's products are not listed.
Hasti Mohsenin, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
TVMV protein fused to a C-terminal His6-tag was mixed with spherical magnetic Ni2+ -NTA functionalized agarose beads (Cube Biotech, Cat. -No. 31201). The binding capacity of the material is reported as 80 mg mL-1 of the settled beads by the manufacturer ...
-
No products found
because this supplier's products are not listed.
Cathal Meehan, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Recombinant IL-6 with an N-terminal his tag (Fitzgerald, Biosynth Ltd.) was immobilized on His-Pur™ Ni-NTA Resin (ThermoScientific ...
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No products found
because this supplier's products are not listed.
Paresh P Kulkarni, et al.,
bioRxiv - Cell Biology 2023
Quote:
Protein C activity in WT and Apoh-/- plasma was performed using the Chromogenix Coamatic Protein C activity kit (Diapharma). Briefly ...
-
No products found
because this supplier's products are not listed.
Marina Chan, et al.,
bioRxiv - Systems Biology 2021
Quote:
... Recombinant purified N-terminal domain (NTD) of the SARS-CoV-2 was obtained from Leinco Technologies Inc (Cat #S853 ...
-
No products found
because this supplier's products are not listed.
MJ Mahesh Kumar, et al.,
bioRxiv - Physiology 2022
Quote:
... 5 μl blood was collected from tail bleed and measured by using HbA1c Nycocard 24T kit according to manufacturer’s protocol and monitored in Nyco Card reader II (Alere Technologies AS ...
-
No products found
because this supplier's products are not listed.
Huilei Wang, et al.,
bioRxiv - Physiology 2023
Quote:
Hypertension was induced by Angiotensin II infusion (Ang II; 1000 ng/kg/min) via osmotic mini pumps (Durect Corp) in male and female WT and Loxl2+/-mice (Fig 1A ...
-
No products found
because this supplier's products are not listed.
Jessica Moretti, et al.,
bioRxiv - Neuroscience 2022
Quote:
... rabbit anti-c-Fos (1:2000, EnCor Biotechnology Inc., RPCA-c-Fos), 3% normal goat serum ...
-
No products found
because this supplier's products are not listed.
Yilin Wang, et al.,
bioRxiv - Immunology 2021
Quote:
... Mice were immunized with type II bovine collagen (MD Biosciences) emulsified in complete Freund’s adjuvant (CFA ...
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No products found
because this supplier's products are not listed.
Thekla Cordes, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... The cell suspension was centrifuged at 20,000 g for 10 min at 4 °C and 20 µl of the supernatant was used for protein quantification using BCA protein assay kit (Cat. #G1002, Lamda Biotech. Inc) as per manufacturer’s instruction.
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No products found
because this supplier's products are not listed.
Piyakarn Boontem, Tetsumori Yamashima,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... rabbit anti-human activated μ-calpain antibody (PEPTIDE Institute, Japan) at 1:250 overnight ...
-
No products found
because this supplier's products are not listed.
Mohammed N.A. Siddiquey, et al.,
bioRxiv - Microbiology 2020
Quote:
... and human embryonic kidney 293T cells were purchased from Genhunter Corp ...
-
No products found
because this supplier's products are not listed.
Andrew LaPoint, et al.,
bioRxiv - Physiology 2023
Quote:
... and cholesterol (2%) (HFHF-C, Research Diets, D09100310) diet or a matched sucrose ...
-
No products found
because this supplier's products are not listed.
Soumya Mukherjee, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Commercial pooled human plasma was purchased from Affinity Biologicals (Ancaster, ON). Purified human neutrophils ...
-
No products found
because this supplier's products are not listed.
Zhenghao Chen, et al.,
bioRxiv - Systems Biology 2021
Quote:
... Samples were genotyped using the 143,259-probe GigaMUGA array from the Illumina Infinium II platform (Morgan et al., 2015) by NeoGen Corp ...
-
No products found
because this supplier's products are not listed.
James Young, et al.,
bioRxiv - Microbiology 2019
Quote:
... or 12 h light/12 h dark cycle in ASPII N+ or ASP II N- (nitrogen-containing or nitrogen-free media) were transferred into a 20-ml glass serum bottle (Wheaton) respectively ...
-
No products found
because this supplier's products are not listed.
Colin K.W. Lim, et al.,
bioRxiv - Bioengineering 2022
Quote:
Harvested cells or nuclei were strained using a 35 μm filter and sorted using a BD FACSAria II Cell Sorter (Roy J. Carver Biotechnology Center Flow Cytometry Facility ...
-
No products found
because this supplier's products are not listed.
Yuxin Lin, et al.,
bioRxiv - Biophysics 2024
Quote:
... The sample was then positioned onto a heated stage (37 °C. Heating elements: Bioscience Tools, TC-E35 ...
-
No products found
because this supplier's products are not listed.
Eva Perez-Martin, et al.,
bioRxiv - Zoology 2021
Quote:
A commercial kit (Life Diagnostics, Inc) specific for bovine and based on a sandwich ELISA was used for the quantitative determination of haptoglobin in buffalo serum following the instructions ...
-
No products found
because this supplier's products are not listed.
Xiaoquan Zhu, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and concentrated using ViraTrap lentivirus purification kit (Biomiga). NIH3T3 or RWPE-1 cells or PC3 or LNCaP at 90% confluence were infected with Lenti-FOXP2 ...
-
No products found
because this supplier's products are not listed.
Roie Cohen, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Samples were then incubated overnight at 4°C in the appropriate primary antibody diluted in antibody diluent buffer (GBI labs cat: E09-300). Following 3 washes in PBS ...
-
No products found
because this supplier's products are not listed.
Saulius Vainauskas, et al.,
bioRxiv - Biochemistry 2021
Quote:
... and a C-terminal fluorescein amidite group (FAM) was obtained through custom synthesis (Bio-Synthesis Inc., Lewisville, TX). To generate a glycopeptide substrate containing asialylated TF-antigen (Gal-β1,3-GalNAc) ...
-
No products found
because this supplier's products are not listed.
Marc Sunden, et al.,
bioRxiv - Biochemistry 2023
Quote:
A peptide (NH2-KKKYPGGSTPVSSANMM-COOH) containing an O-GlcNAcylation site of human Casein kinase II subunit alpha (underlined sequence) was custom synthesized by Nordic BioSite. A mixture of 6 mM glutaraldehyde ...
-
No products found
because this supplier's products are not listed.
Kathleen M. Mulvaney, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... the N-terminal tag was removed by incubation with HRV 3C protease (AG Scientific) and further purified by Superose 6 column ...
-
No products found
because this supplier's products are not listed.
Min Pan, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Cas9 activity was tested using the CRISPRtest™ Functional Cas9 Activity Kit for Human Cells (Cellecta, #CRTEST). The clone with normal viability and high Cas9 activity (> 95% ...
-
No products found
because this supplier's products are not listed.
Grace Y. Liu, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Micropropagation Agar-Type II from Caisson Laboratories; rapamycin from LC Laboratories ...
-
No products found
because this supplier's products are not listed.
Barbara Ciralli, et al.,
bioRxiv - Neuroscience 2023
Quote:
... cannabinol (Cerilliant C-046) and CBD (Cerilliant C-045 ...
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Cat# SC22000,
Coupling buffer+ Washing buffer + EDC, USD $76.00/KIT
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Alexandra Grubman, et al.,
bioRxiv - Neuroscience 2019
Quote:
... were transfected for 48 h with dox-inducible GFP-tagged Gateway-generated Piggybac expression constructs with inserted cDNA encoding human HIF1A and/or ELF3 open reading frames using Glial Mag transfection kit (Oz Biosciences, #GL00500) according to manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Miguel A. Olivencia, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
25-hydroxyvitamin D and testosterone levels were measured in rat citrated plasma and human EDTA plasma using a General 25-Hydroxyvitamin D3 (HVD3) ELISA Kit (Reddot Biotech Inc., Kelowna, Canada) and Testosterone ELISA DE1559 (Demeditec ...
-
No products found
because this supplier's products are not listed.
Ainelen Piazza, et al.,
bioRxiv - Microbiology 2022
Quote:
The quantification of c-di-GMP levels was performed using the Cyclic-di-GMP Assay Kit from Lucerna (Catalog Number: 200-100). Cultures of the strains were adjusted to OD600 = 0.2 and set up for the assay in 30-µl aliquots along with assay reagents and serially diluted c-di-GMP standards ...
-
No products found
because this supplier's products are not listed.
Vaibhav Sidarala, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Anti-human TFAM (1:1000, PhosphoSolutions, Catalog# 1999-hTFAM), Anti-mouse TFAM (1:1000 ...
-
No products found
because this supplier's products are not listed.
Takahiro Sanada, et al.,
bioRxiv - Microbiology 2022
Quote:
... AB human serum (Pel-Freez Biologicals, Rogers, AR, USA) was used as control serum.
-
No products found
because this supplier's products are not listed.
Galit H. Frydman, et al.,
bioRxiv - Immunology 2019
Quote:
... 10 µL of cells were then imaged on a disposable C-Chip hemocytometer (In Cyto, SKC, Inc. C-Chip) using a 10X and 20X objective on a Nikon TiE fluorescent microscope ...
-
No products found
because this supplier's products are not listed.
Wu Liu, et al.,
bioRxiv - Microbiology 2019
Quote:
... equipped with a ProScan II motorized flat top stage (Prior Scientific, USA). With this setup ...
-
No products found
because this supplier's products are not listed.
Wei Zhang, et al.,
bioRxiv - Cell Biology 2023
Quote:
... the human multimeric vitronectin solution (Molecular Innovations Inc, HVN-U) was adjusted to a concentration of 1 mg/mL and then its buffer was exchanged to 0.1 M sodium bicarbonate using a Zeba spin desalting column (Thermo Scientific ...
-
No products found
because this supplier's products are not listed.
Julia L. Daiß, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Top2a was obtained from Inspiralis (c/n HT210). Proteins were incubated together in pulldown buffer (25 mM TrisHCl pH7.9 ...
-
No products found
because this supplier's products are not listed.
Xiao Wu, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... anti-cytochrome c (1:1000, Bioworld Technology, China). The blots were thoroughly washed with TBST and incubated at 37 °C with the secondary antibody in TBST containing 5% skimmed milk powder for 1 h ...
-
No products found
because this supplier's products are not listed.
Cinzia Klemm, Gudjon Olafsson, Peter H Thorpe,
bioRxiv - Cell Biology 2023
Quote:
3xFLAG-tagged Mif2CENP-C cells were harvested after 4 hours of growth at restrictive (37°C) or permissive (23°C) temperature and whole cell lysates were prepared using glass beads and a cell disrupter (Scientific Industries Inc.) in Laemmeli buffer containing an EDTA-free protease inhibitor cocktail (Thermo Fisher) ...
-
No products found
because this supplier's products are not listed.
Jeanette M. Metzger, et al.,
bioRxiv - Neuroscience 2022
Quote:
GFP-expressing human embryonic kidney cells (i.e., GFP-HEK cells, GenTarget Inc.) were used as an RNP delivery cell model ...
-
No products found
because this supplier's products are not listed.
Whasil Lee, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... then next the Transcription factor (TF) Activation Profiling Plate Array II (FA-1002, Signosis, CA) was used to monitor 96 TFs simultaneously ...
-
No products found
because this supplier's products are not listed.
Ghulam Destgeer, et al.,
bioRxiv - Bioengineering 2020
Quote:
... particles were incubated with varying concentrations of human recombinant NT-proBNP (HyTest, Finland) for 1 hr with subsequent washing ...
-
No products found
because this supplier's products are not listed.
Margaret M. McDaniel, Vitaly V. Ganusov,
bioRxiv - Immunology 2019
Quote:
... We fitted either recirculation model (panels A&C, see eqns. (3)–(9) ...
-
No products found
because this supplier's products are not listed.
Fangyuan Ding, et al.,
bioRxiv - Systems Biology 2020
Quote:
... were coated with 5 ug/ml Human Fibronectin (Oxford Biomedical Research, Rochester Hills, MI) in PBS buffer for 1hr at room temperature ...
-
No products found
because this supplier's products are not listed.
Jan Stephan Wichers, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... secondary IgG antibody (goat anti-human IgG QDot™800, Grace Bio-Labs #110635), secondary IgM antibody (biotin-SP-conjugated goat anti-human IgM ...
-
No products found
because this supplier's products are not listed.
Xian Zhou, et al.,
bioRxiv - Immunology 2020
Quote:
... Human B cell proliferation was measured by 3[H]-thymidine incorporation (1 μCi/mL) (American Radiolabeled Chemicals).
-
No products found
because this supplier's products are not listed.
Xin Wang, et al.,
bioRxiv - Immunology 2022
Quote:
Mouse trachea and human ALI culture samples were harvested and fixed with 4% PFA (BM-155, Boston BioProducts) overnight at 4 °C ...
-
No products found
because this supplier's products are not listed.
Karsten Eichholz, et al.,
bioRxiv - Immunology 2021
Quote:
... 7-aminoactinomycin D (7-AAD) (Becton-Dickinson Pharmigen) and analyzed on a FACS Canto II (Becton-Dickinson Pharmigen) or NovoCyte (ACEA Biosciences) flow cytometer.
-
No products found
because this supplier's products are not listed.
Embriette R. Hyde, Hiram Lozano, Steven Cox,
bioRxiv - Microbiology 2020
Quote:
... Chilled (4°C) and pre-reduced anaerobically sterilized (PRAS) dilution blank medium (AS-9183, Anaerobe Systems) was added at a volume of 2:1 (2mL per gram ...
-
No products found
because this supplier's products are not listed.
Luca Valzania, Aya Alami, Pierre Léopold,
bioRxiv - Developmental Biology 2023
Quote:
... using the 20-Hydroxyecdysone ELISA kit (Bertin bioreagent, #A05120) as recommended by the manufacturer ...
-
No products found
because this supplier's products are not listed.
Yuka Sakata, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Commercially-available clinical-grade enzyme-linked immunosorbent assay (ELISA) kits (DRG International) were used to detect pregnenolone (EIA-4170) ...