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... membranes were stripped at 4°C in agitation using Antibody stripping buffer 1X for 10 minutes (Gene Bio-Application). Protein bands were quantified using ImageJ software ...
Ellen M. Rodberg, et al.,bioRxiv - Neuroscience 2022
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... and administered the analgesic/anti-inflammatory Metacam (1 mg/kg, IP, Henry Schein Animal) and antibiotic cefazolin (0.33mg, IM, McKesson, Irving TX) prior to surgery ...
Mark A. Skylar-Scott, et al.,bioRxiv - Bioengineering 2020
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Wild type PGP1-iPSCs were dissociated using EDR and seeded into anti-adhesive treated V-bottom 96-well plates (Globe Scientific, #120130) at a density of 1,000 cells/96-well well in EBCM + 10 μM Y-27632 for 12 h to generate organoid cores.