-
No products found
because this supplier's products are not listed.
Luis F. Garcia-Alles, et al.,
bioRxiv - Biochemistry 2019
Quote:
... protein tags were removed by incubation overnight at 25°C with turbo TEV protease (GenWay, 5 μg/mL final) in 50 mM Tris pH 8.0 / 300 mM NaCl /2 mM DTT/ 1 mM EDTA ...
-
No products found
because this supplier's products are not listed.
Rhett A. Snyder, et al.,
bioRxiv - Microbiology 2019
Quote:
... Quantification of c-di-GMP in sample extracts was determined using a standard curve generated from chemically synthesized c-di-GMP (AXXORA). The standard curve solutions were prepared using twofold serial dilutions of c-di-GMP (1.25 μM – 19 nM ...
-
No products found
because this supplier's products are not listed.
Tian Yu, et al.,
bioRxiv - Neuroscience 2020
Quote:
We utilized Mouse c-Fos antibody (1:1000, PhosphoSolutions; Cat# ...
-
No products found
because this supplier's products are not listed.
Eriko Ohgitani, et al.,
bioRxiv - Microbiology 2021
Quote:
... and RNA was extracted using TRI Reagent(C) LS (Molecular Research Center, Inc. ...
-
No products found
because this supplier's products are not listed.
Andrew I. Wong, et al.,
bioRxiv - Microbiology 2022
Quote:
... samples were separated on a Cogent Diamond Hydride Type C column (Microsolv Technologies). The mobile phase consisted of solvent A (ddH2O with 0.2% formic acid ...
-
No products found
because this supplier's products are not listed.
Amitabh Das, et al.,
bioRxiv - Immunology 2020
Quote:
... Serum C-terminal fragments of CTX were quantified using the RatLaps EIA assay (Immunodiagnostics Systems) according to the manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Genjiro Suzuki, et al.,
bioRxiv - Neuroscience 2020
Quote:
... for 60 minutes at 37°C or 50 μM fluorogenic substrate (boc-LRR-mca, Peptide Institute) for 10 minutes at 37°C ...
-
No products found
because this supplier's products are not listed.
Ryutaro Ariyoshi, et al.,
bioRxiv - Biochemistry 2023
Quote:
... and MTG mutant was purified with size exclusion chromatography using ProteoSEC-D 16/60 6-600 HR (Protein Ark) with PBS (pH 7.4 ...
-
No products found
because this supplier's products are not listed.
Rossana Girardello, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Cells were incubated for 30 min at 37°C in media containing 0.5 mM biotin phenol (Iris Biotech). After 2 washes in PBS ...
-
No products found
because this supplier's products are not listed.
Kellie A. Cotter, et al.,
bioRxiv - Genomics 2022
Quote:
... to reduce uncapped RNAs to 5′ hydroxyls and make them incapable of ligating to 5′ adaptor and Cap-Clip (catalog no. C-CC15011H; Cambio) to remove the 5′ cap of transcripts that had undergone guanylation and allow them to be incorporated into the library through 5′ adapter ligation ...
-
No products found
because this supplier's products are not listed.
Manuel Göpferich, et al.,
bioRxiv - Neuroscience 2020
Quote:
... human FGF (20 ng/μl, ReliaTech) and human EGF (Promokine) ...
-
No products found
because this supplier's products are not listed.
Emma G. Bouck, et al.,
bioRxiv - Pathology 2020
Quote:
... Cadherin-6 expression was quantified using Quantum Simply Cellular beads (Bangs Laboratories, Inc) to generate a standard curve of antibody binding sites as previously described.[10–12]
-
No products found
because this supplier's products are not listed.
Laura Zein, et al.,
bioRxiv - Cell Biology 2023
Quote:
Samples were loaded onto 75 µm x 15 cm fused silica capillaries (custom-made) packed with C18AQ resin (Reprosil-Pur 120, 1.9 µm, Dr. Maisch HPLC) using an Easy-nLC1200 liquid chromatography ...
-
No products found
because this supplier's products are not listed.
Thekla Cordes, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... The cell suspension was centrifuged at 20,000 g for 10 min at 4 °C and 20 µl of the supernatant was used for protein quantification using BCA protein assay kit (Cat. #G1002, Lamda Biotech. Inc) as per manufacturer’s instruction.
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No products found
because this supplier's products are not listed.
Ali Khateb, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... The plates were briefly centrifuged at 1000 rpm and incubated at 37°C with 5% CO2 for an additional 6 days using MicroClime Environmental lids (Labcyte, San Jose, CA). Plates were placed at room temperature for 30 min to equilibrate ...
-
No products found
because this supplier's products are not listed.
Xiaoke Chi, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Membranes were pelleted by centrifugation at 13000g for 10 min and cytochrome c release was analyzed by immunoblotting using a sheep anti-cytochrome c antibody (Capralogics). Mitochondria from embryonic mouse brains for BH3 profiling experiments were prepared from ~20 mouse embryos ...
-
No products found
because this supplier's products are not listed.
Julie Van Coillie, et al.,
bioRxiv - Immunology 2022
Quote:
... IL-6 levels in the supernatant were measured by enzyme-linked immunosorbent assay (ELISA) using IL-6 CT205-c and CT205-d antibody pair (U-CyTech, Utrecht, the Netherlands) as described previously (1).
-
No products found
because this supplier's products are not listed.
Leslie Duplaquet, et al.,
bioRxiv - Cancer Biology 2024
Quote:
The following chemicals (stored at −20°C or −80°C) were added to cell culture where indicated: BVdU was purchased from Chem-Impex International Inc. ...
-
No products found
because this supplier's products are not listed.
Mia P. Sams, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Samples were denatured at 100°C and separated on 10% SDS-PAGE gels with BLUeye Prestained Protein Ladder (FroggaBio, Concord ON). Protein was then semi-dry transferred to a PVDF membrane and incubated in Li-Cor Intercept® Blocking Buffer (Cedarlane ...
-
No products found
because this supplier's products are not listed.
Irene Pereira de Sousa, et al.,
bioRxiv - Bioengineering 2019
Quote:
... 50 mg of dried MSPs were degassed for 1 h at 100 °C with nitrogen and then analyzed by nitrogen sorption at −196 °C (TriStar 3000, Micromeritics, Norcross, GA, USA). The morphology of MSPs was assessed by SEM (Quanta 200F ...
-
No products found
because this supplier's products are not listed.
Changyang Linghu, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
The DNAs encoding the protein motifs used in this study were mammalian-codon optimized and synthesized by Epoch Life Science and then cloned into mammalian expression backbones ...
-
No products found
because this supplier's products are not listed.
Nidhi Nandu, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... NATtrol MTB verification panel was purchased from ZeptoMetrix (Cat# NATMTBP-C).
-
No products found
because this supplier's products are not listed.
Gabriela Zurawska, et al.,
bioRxiv - Cell Biology 2023
Quote:
... mice received i.v.solution of liposomes containing clodronic acid (LIPOSOMA, #C-SUV-005) (5 ml/kg ...
-
No products found
because this supplier's products are not listed.
Yejin Jang, et al.,
bioRxiv - Microbiology 2020
Quote:
... Oseltamivir carboxylate (OSV-C) was purchased from United States Biological (Swampscott, MA, USA). Marine microalgae-derived sulfated polysaccharide p-KG03 was provided and characterized by Dr ...
-
No products found
because this supplier's products are not listed.
Elisangela Bressan, et al.,
bioRxiv - Genomics 2023
Quote:
... Primary antibodies were applied overnight at 4°C and included TH (Pel-Freez Biologicals #P40101 and Merck Millipore #AB9702 ...
-
No products found
because this supplier's products are not listed.
Hitika Gulabani, et al.,
bioRxiv - Plant Biology 2021
Quote:
... rinsed and incubated overnight at 4°C with indicated primary antibodies [anti-SNC1 (Abiocode; R3588-1), anti-PR1 ...
-
No products found
because this supplier's products are not listed.
Maiken Engelbrecht Petersen, et al.,
bioRxiv - Microbiology 2023
Quote:
... Compounds that incorporated this or a similar motif were purchased from Enamine Ltd (Kyiv ...
-
No products found
because this supplier's products are not listed.
Chuan Xu, et al.,
bioRxiv - Immunology 2023
Quote:
Rabbit polyclonal antibodies against human or murine IFNε proteins were generated by using peptides derived from IFNε protein sequences (Lampire Biological Laboratories (Pipersville, PA). The specificity of antibodies was determined by western blot analysis ...
-
No products found
because this supplier's products are not listed.
Benjamin L. Woods, et al.,
bioRxiv - Cell Biology 2020
Quote:
Cells were grown to mid-log phase at 24°C in Complete Synthetic Medium (CSM, Sunrise Science Products) supplemented with 0.67% yeast nitrogen base ...
-
No products found
because this supplier's products are not listed.
Sami Saarenpää, et al.,
bioRxiv - Genomics 2022
Quote:
The leaves stored at -80°C were immersed in 50% Optimal Cutting Compound (OCT, Sakura) in PBS (Medicago). Embedded samples were frozen in a cryotome (Cryostar NX70 ...
-
No products found
because this supplier's products are not listed.
Logan T. Blancett, et al.,
bioRxiv - Microbiology 2023
Quote:
... Fc receptors were blocked for 10 minutes with CD32/CD16 mAb (Leinco Technologies, Inc.). Cells were incubated with Zombie UV™ Fixable Viability Dye (BioLegend ...
-
No products found
because this supplier's products are not listed.
Hong Zheng, et al.,
bioRxiv - Microbiology 2021
Quote:
... the cells were labeled with 1:500 rabbit anti-CSP overnight at 4°C and 1:100 Red donkey anti-rabbit secondary antibody (Abbkine) for 1 h at room temperature ...
-
No products found
because this supplier's products are not listed.
Huibao Feng, et al.,
bioRxiv - Genetics 2022
Quote:
... for 1 h at 37°C for recovery and then plated onto 37 Φ150 LB agar plates containing kanamycin with an EasySpiral Pro (Interscience). Generally ...
-
No products found
because this supplier's products are not listed.
Saki Kiuchi, et al.,
bioRxiv - Biochemistry 2024
Quote:
RAW 264.7 cells were grown at 37 °C in DMEM (Dulbecco’s modified Eagle medium) supplemented with 10% EquaFETAL (Atlas Biologicals, Inc.) in a humidified 5% CO2 atmosphere ...
-
No products found
because this supplier's products are not listed.
Lina Freage, et al.,
bioRxiv - Biochemistry 2020
Quote:
... while OSJ-T3-OMe was synthesized by using the 2’-OMe-Ac-C-CE and 2’-OMe-U-CE phosphoramidites (Glen Research) to modify the 40th and 41st bases (Glen Research) ...
-
No products found
because this supplier's products are not listed.
Oscar Solis, et al.,
bioRxiv - Immunology 2022
Quote:
... Sections were rinsed and incubated overnight at 4°C in the secondary antibody Nanogold-Fab’ goat anti-rat-IgG (1:100, Nanoprobes, #2008) for ERα protein detection ...
-
No products found
because this supplier's products are not listed.
Stephanie Braun-Galleani, et al.,
bioRxiv - Genetics 2019
Quote:
... The plate was incubated for 4 hours at 30 °C to allow asci to swell and become more visible under the 40X lens of a SporePlay+ dissection microscope (Singer Instruments). Whole asci were picked up and placed individually on the YPD plate ...
-
No products found
because this supplier's products are not listed.
W. Coles Keeter, et al.,
bioRxiv - Immunology 2023
Quote:
... 8-12-week-old male mice were placed on a high fat diet with 0.15% added cholesterol (HFD-C, fat: 60% kcal; carbohydrate: 26% kcal, Bio-Serv F3282) for 28 weeks until sacrifice.
-
No products found
because this supplier's products are not listed.
Chloe C. Ence, et al.,
bioRxiv - Microbiology 2024
Quote:
... The cultures were incubated at 37°C with 5% CO2 and routinely checked for mycoplasma contamination using the e-Myco Plus kit from Intron Biotechnology. Human hepatocellular carcinoma cells (HepG2 ...
-
No products found
because this supplier's products are not listed.
Zainul S. Hasanali, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... 100μL of cell suspension was removed to a 1.2mL eppendorf tube and placed at -20°C for subsequent pathogen testing (IDEXX -hIMPACT panel). Remaining cells were spun down and supernatant removed ...
-
No products found
because this supplier's products are not listed.
Jiao Wang, et al.,
bioRxiv - Immunology 2020
Quote:
... (6) was purchased from GenTarget Inc.
-
No products found
because this supplier's products are not listed.
Pranjal Biswas, Dennis J. Stuehr,
bioRxiv - Cell Biology 2022
Quote:
... the reported half-life of NOC-18 at pH 7 at 37 °C was 13 h (Dojindo Molecular Technologies Inc. website for NOC-18).
-
No products found
because this supplier's products are not listed.
Chao Ye, et al.,
bioRxiv - Systems Biology 2023
Quote:
... Genomic DNA extraction was done by either boiling cells in 25 μL of 20 mM NaOH at 95 °C for 15 min or by using the Direct DNA Extraction Kit (Bacteria) (Norgen Biotek, Thorold, ON, Canada) following the manufacturer’s manual.
-
No products found
because this supplier's products are not listed.
Jack P. K. Bravo, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and nanoPOP-6 polymer (Molecular Cloning Laboratories). The oven temperature was set to 65°C ...
-
No products found
because this supplier's products are not listed.
Ashley Maynard, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... + 6% FBS (Omega Scientific, Inc, FB-11) and spun in the centrifuge at 500xg for 5 minutes ...
-
No products found
because this supplier's products are not listed.
Maitreyi S. Joshi, et al.,
bioRxiv - Systems Biology 2022
Quote:
... atto-590azide (6 mM, ATTO-TEC GmbH, Siegen, Germany) dissolved in DMSO was mixed with aqueous solution of click-chemistry grade CuSO4 (40mM ...
-
No products found
because this supplier's products are not listed.
Yuichi Mitsui, et al.,
bioRxiv - Immunology 2022
Quote:
Human PBMCs were purchased from Precision for Medicine, Inc ...
-
Cat# SC22000,
Coupling buffer+ Washing buffer + EDC, USD $76.00/KIT
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Tamadher A. Alghamdi, et al.,
bioRxiv - Physiology 2022
Quote:
Enzymatic X-gal staining of pancreas cryosections (5 μm thickness) was performed using an X-Gal Staining Kit (Cat#GX10003, Oz Biosciences INC, San Diego, CA, US) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Ava P. Soleimany, et al.,
bioRxiv - Bioengineering 2020
Quote:
Fresh frozen human PCa TMAs (BioChain Institute, Inc.; T6235201) were air dried and fixed in ice-cold acetone for 10 minutes ...
-
No products found
because this supplier's products are not listed.
Pedro A. Avila-Lopez, et al.,
bioRxiv - Genomics 2024
Quote:
... The cells were cross- linked with 6 mM disuccinimidyl glutarate (DSG; ProteoChem) for 30 min followed by crosslinking with 1% formaldehyde for an additional 10 min at room temperature and quenched with 125 mM glycine (Fisher Scientific ...