-
No products found
because this supplier's products are not listed.
Yan Qi, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... and the human herpes simplex virus 5 puromycin resistance marker (Clontech).
-
No products found
because this supplier's products are not listed.
Drice Challal, et al.,
bioRxiv - Genetics 2023
Quote:
... we first deleted the ORF with a cassette containing the hygromycin resistance marker (hphRMX) fused to the herpes simplex virus thymidine kinase-encoding gene (HSV-TK) from the pFA6a-HyTkAX vector (Addgene plasmid # 73898 ...
-
No products found
because this supplier's products are not listed.
Erica Bello, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and a panel of ProcartaPlex Human Simplex (Invitrogen) beads ...
-
No products found
because this supplier's products are not listed.
Susanne Wolf, et al.,
bioRxiv - Immunology 2020
Quote:
The HSV-seropositivity status of donors completing the survey was confirmed by ELISA using the anti-herpes simplex virus type 1 and 2 IgG human ELISA kit (abcam, Cambridge, United Kingdom) according to manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Hadj S Aoued, et al.,
bioRxiv - Zoology 2019
Quote:
... The zygotes were cultured in potassium-supplemented simplex optimised medium (KSOM, EMD Millipore) at 37°C in 5% CO2 incubator and then injected using a microinjector setup (XenoWorks Micromanipulator and Digital Microinjector by Shutter Instruments) ...
-
No products found
because this supplier's products are not listed.
Wenqing Ma, et al.,
bioRxiv - Microbiology 2024
Quote:
... Herpes simplex virus 1 (ATCC-2011-9 strain; VR-1789) was obtained from American Type Culture Collection (ATCC).
-
No products found
because this supplier's products are not listed.
N.D. Carvalho, et al.,
bioRxiv - Microbiology 2022
Quote:
Total RNA was extracted from 200μL of VERO cells culture infected by herpes virus (treated or not with hemolymph) using the MagNA Pure extractor (Roche, Basel, Switzerland). The Herpes simplex virus quantitative real time PCR assay was performed was described by Read et al. ...
-
No products found
because this supplier's products are not listed.
Ashley M Ngo, et al.,
bioRxiv - Microbiology 2019
Quote:
... Dengue virus NS4B protein (Genetex, GTX124250) at 1:1000 ...
-
No products found
because this supplier's products are not listed.
Carolina Q. Sacramento, et al.,
bioRxiv - Microbiology 2020
Quote:
... Virus RNA virus was extracted by Qiamp viral RNA (Qiagen) and quantified using Qbit 3 Fluorometer (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Phillip Shults, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... These markers were amplified in standard simplex PCR conditions using a Bio-Rad T100 thermal cycler (Bio-Rad, Pleasanton, CA, USA). Each PCR reaction contained 2.0 μl of DNA ...
-
No products found
because this supplier's products are not listed.
Nipun Verma, et al.,
bioRxiv - Genetics 2024
Quote:
... Virus was concentrated using PEG virus precipitation (Promega # V3011). In brief ...
-
No products found
because this supplier's products are not listed.
Youjia Guo, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... Cumulus cells were removed by briefly culturing the zygotes in potassium simplex optimization medium (KSOM) (catalog no. MR-101-D, Merck) supplemented with 0.3 µg.µL-1 hyaluronidase (catalog no ...
-
No products found
because this supplier's products are not listed.
Brandon J. Beddingfield, et al.,
bioRxiv - Microbiology 2021
Quote:
... of SARS-CoV-2 spike protein to Angiotensin Converting Enzyme (ACE2) was assessed via the Surrogate Virus Neutralization Test (GenScript# L00847) using the included kit protocol modified per the following ...
-
No products found
because this supplier's products are not listed.
Yunlong Cao, et al.,
bioRxiv - Immunology 2022
Quote:
... G*ΔG-VSV virus (VSV G pseudotyped virus, Kerafast) is used to infect 293T cells (American Type Culture Collection [ATCC] ...
-
No products found
because this supplier's products are not listed.
Rebecca P. Sumner, et al.,
bioRxiv - Immunology 2023
Quote:
... Sendai virus was from Charles River Laboratories ...
-
No products found
because this supplier's products are not listed.
Angela Ma, et al.,
bioRxiv - Microbiology 2022
Quote:
... DFA kits were used to test for herpes simplex virus types 1 and 2 (D3 DFA HSV Identification and Typing kit, Quidel, San Diego, CA), varicella-zoster virus (Light Diagnostics ...
-
No products found
because this supplier's products are not listed.
Daniel Perez-Zsolt, et al.,
bioRxiv - Microbiology 2021
Quote:
... Virus particles were immunostained with rabbit anti-SARS-CoV-2 N protein (Sino Biological) or rabbit anti-GM1 Ab (Abcam ...
-
No products found
because this supplier's products are not listed.
Fabian Blombach, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... using the Vaccinia virus Capping enzyme (NEB) and biotinylated RNA species were subsequently enriched by affinity purification using streptavidin beads yielding 0.6 to 1.3% of the sRNA preparation ...
-
No products found
because this supplier's products are not listed.
Kristina Lanko, et al.,
bioRxiv - Microbiology 2020
Quote:
Viral RNA was isolated from virus stocks with Nucleospin RNA virus (Macherey-Nagel) according to manufacturer protocol ...
-
No products found
because this supplier's products are not listed.
Ronnie Blazev, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Virus was purified by heparin affinity chromatography on an Akta FPLC (HiTrap, GE Healthcare), ultra-centrifuged overnight and resuspended in sterile Ringer’s solution ...
-
No products found
because this supplier's products are not listed.
Joseph M Gibbons, et al.,
bioRxiv - Microbiology 2019
Quote:
... and the vesicular stomatitis virus G protein (VSV-G) expression vector pVPack-VSV-G (Stratagene). After 72 hours ...
-
No products found
because this supplier's products are not listed.
Kelly Mitchell, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and virus was concentrated with PEG-it Virus Precipitation solution (System Biosciences). SORE6-GFP virus was added to CSCs plated on Geltrex ...
-
No products found
because this supplier's products are not listed.
Chen-xi Zhang, et al.,
bioRxiv - Immunology 2020
Quote:
The serum levels of a total of 12 virus-related cytokines were measured by a bead-based immunoassay panel (Mouse Anti-Virus Panel, Cat No: 740622, Biolegend, USA). The BALF levels of a total of 12 inflammatory cytokines were measured by a bead-based immunoassay panel (Mouse Inflammation Panel ...
-
No products found
because this supplier's products are not listed.
Eleonora Sementino, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Then virus particles and polybrene (sc-134220; Santa Cruz) at a concentration of 2 μg/ml were added to the plate ...
-
No products found
because this supplier's products are not listed.
Gloria P. Larson, et al.,
bioRxiv - Microbiology 2019
Quote:
Multicycle replication infections were performed by inoculating A549 cells at a multiplicity of infection (MOI) of 0.01 using virus diluted in virus growth media (VGM) (DMEM supplemented with penicillin/streptomycin [Corning cellgro] ...
-
No products found
because this supplier's products are not listed.
Jonathan Burnie, et al.,
bioRxiv - Microbiology 2021
Quote:
The quantification of HIV-1 p24 capsid protein was performed in captured virus lysates and virus-containing supernatants with the high-sensitivity AlphaLISA p24 detection kit following the manufacturer’s (PerkinElmer) instructions ...
-
No products found
because this supplier's products are not listed.
Alexis S. Gorin, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Virus was injected iontophoretically (Nanoject II, Drummond Scientific) via a glass micropipette (tip diameter ∼15-20 μm ...
-
No products found
because this supplier's products are not listed.
Jiang-Hui Wang, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... The collected virus was filtered (0.45 μm filter, Sartorius) and concentrated using PEG-it overnight at 4°C (SBI Integrated Sciences ...
-
No products found
because this supplier's products are not listed.
G. Scott, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... Reactions were run in simplex at 1 and 10-fold dilutions on a Mastercycler® Nexus Gradient (Eppendorf, Germany). Optimised PCR Ta were compared to Ta=50°C as used by Ollivier et al ...
-
No products found
because this supplier's products are not listed.
S.N. Freda, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Virus was injected using a microsyringe pump (World Precision Instruments); >5 minutes elapsed before the pipette was moved from the ventral injection site to the more dorsal injection site ...
-
No products found
because this supplier's products are not listed.
Olesya N Gusachenko, et al.,
bioRxiv - Microbiology 2021
Quote:
Virus injections of pupae were performed with insulin syringes (BD Micro Fine Plus ...
-
No products found
because this supplier's products are not listed.
Yang Yang, et al.,
bioRxiv - Microbiology 2019
Quote:
... Virus titers were measured using p24 enzyme-linked immunosorbent assays (ELISA) (R&D Systems, MN, USA). CD4+ Jurkat T cells were incubated with NL4-3 or NL4-3dNef viruses at MOI ...
-
No products found
because this supplier's products are not listed.
Kristen Zarrella, et al.,
bioRxiv - Microbiology 2023
Quote:
... Cells were infected (MOI=1) with WT or UL138STOP virus for 48 hours and then processed for CUT&RUN (Cell Signaling Technologies) as per manufacturer’s recommended protocol ...
-
No products found
because this supplier's products are not listed.
Laia Bassaganyas, et al.,
bioRxiv - Cell Biology 2019
Quote:
... the medium was refreshed and after additional 24 h virus was collected and filtered through a 0.45μm low protein binding membrane (VWR International, Radnor, PA, #28145-479). For dCas9-KRAB fusion protein expression and CRISPRi-mediated knockdown ...
-
No products found
because this supplier's products are not listed.
Andrii Slonchak, et al.,
bioRxiv - Microbiology 2021
Quote:
... Virus replication foci were counted using the Image Studio Lite software (v5.2.5, LI-COR, USA), and titres were determined based on dilution factors and expressed as focus forming units per mL (FFU mL^−1).
-
No products found
because this supplier's products are not listed.
Nathaniel Kastan, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Ad-RFP virus (Vector Biolabs) at the same titer were used as a control.
-
No products found
because this supplier's products are not listed.
Déborah Lécuyer, et al.,
bioRxiv - Microbiology 2023
Quote:
... Envelope defective virus was pseudotyped with HU-1 SARS-CoV-2 Spike protein (pLV-Spike, Invivogen) and used for single round infection assays ...
-
No products found
because this supplier's products are not listed.
Lok R. Joshi, et al.,
bioRxiv - Immunology 2021
Quote:
... ultra-centrifugation of virus culuture supernatant and the virus pellet in 30% sucrose cushion gradient were performed using Optima-L 100K ultracentrifuge (Beckman Coulter) at 18,000 RPM for 1.5 hours ...
-
No products found
because this supplier's products are not listed.
Sanjib Saha, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Protein expression was normalized to total protein (DM-TP01, Protein Simple) and compared between cell types.
-
No products found
because this supplier's products are not listed.
Jingyi Guo Fuglstad, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Fluorescent signal from the virus was amplified by immunostaining against Red Fluorescent Protein (RFP) (catalog no.5F8, Chromotek GmbH, Germany), followed by secondary antibody-staining with Alexa 546-tagged Goat Anti-rat IgG (catalog no ...
-
No products found
because this supplier's products are not listed.
Michael B. Fessler, et al.,
bioRxiv - Immunology 2022
Quote:
... The virus sequence was confirmed by Illumina sequencing and did not differ from the parental virus ...
-
No products found
because this supplier's products are not listed.
Wenwei Li, et al.,
bioRxiv - Microbiology 2021
Quote:
... virus containing supernatants were clarified using a 0.45 µM PDVF filter (Pall Corp, NY, USA # 4614) and pelleted by ultracentrifugation on a 15% sucrose cushion before resuspension in culture media to achieve a 20X concentration over the original volume ...
-
No products found
because this supplier's products are not listed.
Filippo Bianchini, et al.,
bioRxiv - Immunology 2022
Quote:
... Avi-tagged SARS-CoV-2 RBD and SD1-RBD (both corresponding to SARS-CoV-2 ancestral virus) were biotinylated using the Biotin-Protein Ligase-BIRA kit according to manufacturer’s instructions (Avidity). Ovalbumin (Sigma ...
-
No products found
because this supplier's products are not listed.
Takanobu Tagawa, et al.,
bioRxiv - Microbiology 2022
Quote:
... The virus pellet was resuspended to EBM2 (Lonza) media ...
-
No products found
because this supplier's products are not listed.
Camilla Schinner, et al.,
bioRxiv - Cell Biology 2021
Quote:
... virus particle containing supernatant was collected and enriched using LentiConcentrator (OriGene). Cells were transduced with the respective concentrated virus particles using 10 µg/mL polybrene (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Paula Lagan, et al.,
bioRxiv - Microbiology 2023
Quote:
... Successful virus isolation was confirmed either by haemagglutination of 0.5% chicken blood cells (Envigo #S.B-0008) or by RRT-PCR.
-
No products found
because this supplier's products are not listed.
Marion Thépaut, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Proteins were detected using InstantBlue protein stain (Expedeon) according to the supplier’s instructions.
-
No products found
because this supplier's products are not listed.
May Daher, et al.,
bioRxiv - Immunology 2020
Quote:
... Cas9 protein (PNA bio) and gRNA were incubated at room temperature for 15 min in a 3:1 reaction with 15μg Cas9 and 5000 ng gRNA (2500ng of sgRNA#1 and 2500ng of sgRNA#2).47 The incubation product (gRNA and Cas9 complex ...
-
No products found
because this supplier's products are not listed.
Qiuli Shi, et al.,
bioRxiv - Microbiology 2021
Quote:
Proteins were labeled using the Monolith NT Protein Labeling Kit RED (NanoTemper Technologies) according to the manufacturer’s protocol and using a buffer consisting of 20 mM HEPES ...
-
No products found
because this supplier's products are not listed.
David Gonzalez-Perez, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Proteins were separated by SDS-PAGE (12% Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) and transferred to PVDF membranes using an iBlot2 Gel Transfer Device (Thermo Fisher Scientific) ...