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One μl of anti-melanopsin conjugated with saporin (Melanopsin-SAP, 400 μg/ml, Advance Targeting System, San Diego, CA) was injected into the vitreous of the right eye of Tbr2TauGFP/+ mice using a 33-gauge NanoFil system (World Precision Instruments ...
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... Cells that bound the non-competing anti-GP38 antibodies were sorted and plated on complete minimal media glucose agar plates minus tryptophan (Teknova). For each library ...
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... LaG-16 anti-GFP nanobodies32 were recombinantly produced in house and labeled with α-Biotin-ω-(succinimidyl propionate) 24(ethylene glycol) (Iris Biotech) as previously described33,34 ...