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One μl of anti-melanopsin conjugated with saporin (Melanopsin-SAP, 400 μg/ml, Advance Targeting System, San Diego, CA) was injected into the vitreous of the right eye of Tbr2TauGFP/+ mice using a 33-gauge NanoFil system (World Precision Instruments ...
Joseph de Rutte, et al.,bioRxiv - Bioengineering 2021
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... antibody atezolizumab and the anti-interleukin 8 receptor beta (IL-8Rb) antibody 10H2 were cloned into the gwiz mammalian expression vector (Genlantis) and used for transient transfection of HEK 293T cells loaded into nanovials ...
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... Cells that bound the non-competing anti-GP38 antibodies were sorted and plated on complete minimal media glucose agar plates minus tryptophan (Teknova). For each library ...
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... LaG-16 anti-GFP nanobodies32 were recombinantly produced in house and labeled with α-Biotin-ω-(succinimidyl propionate) 24(ethylene glycol) (Iris Biotech) as previously described33,34 ...
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Calpain-2 immunostaining was performed using a rabbit anti-human calpain-2 (10 µg/ml, catalog No. RP-3 Calpain-2; Triple Point biologics, Forest Grove, OR). CD68 immunostaining was performed using a rabbit anti-human CD68 antibody (1:200 ...