-
No products found
because this supplier's products are not listed.
Federico Bertoglio, et al.,
bioRxiv - Biochemistry 2020
Quote:
... S1-His or RBD-His were labelled using Monolith NTTM His-Tag Labeling Kit RED-tris-NTA (Nanotemper) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
James P Bridges, et al.,
bioRxiv - Cell Biology 2021
Quote:
HEK293 cells were seeded in 384-well plates (Greiner, #781946) at 30 000 cells/well ...
-
No products found
because this supplier's products are not listed.
Jessica M Snyder, et al.,
bioRxiv - Pathology 2020
Quote:
... 7 Cyp26a1−/−b1−/− (3M, 4F); 7 Cyp26b1−/− (3M ...
-
No products found
because this supplier's products are not listed.
Cheng-Tsung Pan, et al.,
bioRxiv - Evolutionary Biology 2022
Quote:
... A mouse 6X His tag antibody (HRP) (GeneTex Inc.) was then used to recognize Gal3-His at room temperature for 1 hour ...
-
No products found
because this supplier's products are not listed.
Xi Zhang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... or plastic-bottom (Ibidi: DLD1, HEK293) 96-well plates at densities targeting 60-70% confluence at the time of transfection ...
-
No products found
because this supplier's products are not listed.
Brian Belardi, et al.,
bioRxiv - Cell Biology 2019
Quote:
... HEK293 cells were transfected with TransIT-293 (Mirus) according to manufacturer’s instructions with three plasmids ...
-
No products found
because this supplier's products are not listed.
Joseph de Rutte, et al.,
bioRxiv - Bioengineering 2021
Quote:
... or using flow cytometry (HEK293, CytoFlex, Beckman Coulter).
-
No products found
because this supplier's products are not listed.
Mark Borris D. Aldonza, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Fucosylated proteins were eluted twice with 50 uL AAL elution buffer B1 or 40 uL glycoprotein eluting solution for fucose-binding lectins (Vector Laboratories) at 4°C for 1 h per round ...
-
No products found
because this supplier's products are not listed.
Kerstin Voelkl, et al.,
bioRxiv - Neuroscience 2023
Quote:
... mouse anti-His (Dianova, DIA-900-100, 1:1,000), chicken anti-EGFP (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Zachary C. Elmore, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... samples were incubated with primary antibodies to either capsid (mouse monoclonal B1 hybridoma supernatant, 1:250, 65158; Progen), actin (mouse monoclonal anti-beta actin ...
-
No products found
because this supplier's products are not listed.
Sundary Sormendi, et al.,
bioRxiv - Immunology 2020
Quote:
BMDNs were obtained by crushing long bones the bones in 5% FCS using a mortar and either isolated by negative selection using the EasySep Mouse Neutrophil Enrichment Kit (Stemcell Technologies) or by positive selection using biotinylated antibodies (see supplemental data for more details).
-
No products found
because this supplier's products are not listed.
Priyabrata Mohanty, et al.,
bioRxiv - Microbiology 2021
Quote:
... 50 μL mouse monoclonal anti-6X His tagged primary antibody (Calbiochem) was added at a dilution of 1:150 in 0.5 % BSA in PBST in the respective wells and incubated for 2 hours without shaking at room temperature ...
-
No products found
because this supplier's products are not listed.
Vindhya Vijay, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... hnRNPA2/B1 and RALY were purchased from MyBiosource and Ray Biotech ...
-
No products found
because this supplier's products are not listed.
Sarah N. Zvornicanin, et al.,
bioRxiv - Biochemistry 2023
Quote:
HI-Control™ BL21(DE3) (Lucigen) cells were transformed with plasmid and single colonies were picked to start overnight cultures in LB medium supplemented with 50 μg/mL kanamycin at 37 °C ...
-
No products found
Wei Dong, et al.,
bioRxiv - Cell Biology 2020
Quote:
HEK293 cells were cultured in glass bottom dishes (In Vitro Scientific) and were transfected with DNA using X-treme Gene 9 DNA transfection reagent (Sigma Cat# 6365787001) ...
-
No products found
because this supplier's products are not listed.
Pablo Alcón, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... 20 µM His-tagged ubiquitin (Enzo Life Sciences) in a reaction buffer of 50 mM HEPES pH 7.5 ...
-
No products found
because this supplier's products are not listed.
Xinrui Wang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... His-tone H2A.Z (1:200, Active Motif #39013), Nkx2.5 (1:250 ...
-
No products found
because this supplier's products are not listed.
Can Bora Yildiz, et al.,
bioRxiv - Molecular Biology 2023
Quote:
Cells were stimulated with 5 µg/mL of either the recombinant ephrinA5-Fc (#374-EA, Biotechne) or Fc protein (#109-1103, Rockland) as control ...
-
No products found
because this supplier's products are not listed.
Jeremy Krohn, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Primary antibodies were incubated overnight at 4°C in 2% BSA + 10% Fetal Calf Serum (FCS) + 0.3% Triton-X-100: mouse anti GFAP (GFAP; 1:4000; Synaptic Systems Cat# 173 011, RRID:AB_2232308), rabbit anti Mover (Mover ...
-
No products found
because this supplier's products are not listed.
Frederike Klimm, Thomas Speck, Marc Thielen,
bioRxiv - Plant Biology 2023
Quote:
... and any remaining parenchyma tissue material stuck to the lignified structures was gently removed using a brush. The isolated lignified structure was photographed (cf. fig. 4 b1 and d1) (stereomicroscope SZX9, Olympus, Tokio ...
-
No products found
because this supplier's products are not listed.
Simon J. Cleary, et al.,
bioRxiv - Immunology 2024
Quote:
... These sequences were codon-optimized and antibodies were expressed as chimeric hIgG1 with or without Fc point mutations in a HEK293 cell system and purified using protein A and buffer exchange (Absolute Antibody).
-
No products found
because this supplier's products are not listed.
Carolin Fritz, et al.,
bioRxiv - Cell Biology 2024
Quote:
... His (MP Biomedicals) plates ...
-
No products found
because this supplier's products are not listed.
Lei Chang, et al.,
bioRxiv - Genomics 2019
Quote:
... STORM imaging of lamin B1 was done on N-STORM (Nikon, Japan)
-
No products found
because this supplier's products are not listed.
Jia Xuan Leong, et al.,
bioRxiv - Plant Biology 2021
Quote:
... Recombinant His-UBA1 and His-UBC8 were purified using Ni-Ted resin (Macherey-Nagel). Purified proteins were used for in vitro ubiquitination assays ...
-
No products found
because this supplier's products are not listed.
Rose Blake, et al.,
bioRxiv - Microbiology 2023
Quote:
... anti-His monoclonal antibody (Raybiotech) and goat anti-rabbit IgG were used as primary and secondary antibodies (Supplementary Table 1) ...
-
No products found
because this supplier's products are not listed.
Miriam R. Fein, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Fc receptor blocker (Innovex Biosciences), and finally avidin/biotin blocking buffer (Vector Laboratories ...
-
No products found
because this supplier's products are not listed.
Danila Boytsov, et al.,
bioRxiv - Biophysics 2022
Quote:
... the FCS unit (Confocor3, Carl Zeiss, Jena, Germany) of a commercial laser scanning microscope (LSM 510 ...
-
No products found
because this supplier's products are not listed.
Heng Lin, et al.,
bioRxiv - Neuroscience 2023
Quote:
... the immobilized FLAG-NBR1 was incubated with 1.5 μg His-MAPT and 1 μg GST or GST-BAG3 (Abnova H00009531-P01) in the BAG buffer (25 mM HEPES ...
-
No products found
because this supplier's products are not listed.
Eleni Kafkia, et al.,
bioRxiv - Systems Biology 2020
Quote:
... mouse anti-lamin B1 (1:500, Atlas Antibodies, AMAb91251) and anti-mouse Alexa Fluor 555 (1:1000 ...
-
No products found
because this supplier's products are not listed.
Viktória Szentgyörgyi, et al.,
bioRxiv - Neuroscience 2024
Quote:
Fumonisin B1 (FB1, Tocris), deoxynivalenol (DON ...
-
No products found
because this supplier's products are not listed.
Arun Prasath Damodaran, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... mouse anti-His tag (HIS.H8 / EH158, Covalab, 1:2500), mouse anti-FLAG tag (clone M2-F1804 ...
-
No products found
because this supplier's products are not listed.
Li Xu, et al.,
bioRxiv - Bioengineering 2020
Quote:
... Total RNA was extracted from mouse tissues and HEK293 cells using Quick-RNA MiniPrep Kit (ZYMO Research, Irvine, CA). Five μg of treated RNA was used as template for first-strand cDNA synthesis by using RevertAid RT Reverse Transcription Kit (Life Technologies ...
-
No products found
because this supplier's products are not listed.
Pablo Canales-Herrerias, et al.,
bioRxiv - Immunology 2023
Quote:
... Sections were stained with primary antibodies: Goat anti-mouse IgD (goat polyclonal GAM/IgD, FC/7S, Cedarlane Labs), biotin anti mouse GL7 (Biolegend) ...
-
No products found
because this supplier's products are not listed.
Henriette Hoffmann-Veltung, et al.,
bioRxiv - Immunology 2022
Quote:
... 100 µL of either goat anti-mouse IgG (for 6E2 hybridoma cells) or mouse anti-human IgG (for EBV-immortalized human B cells) specific for the Fc region (Mabtech) diluted in sterile PBS at 15 µg/mL were added to wells followed by incubation at 4°C for 24 hours ...
-
No products found
because this supplier's products are not listed.
Prashant P. Damke, et al.,
bioRxiv - Microbiology 2022
Quote:
... using a microplate reader (BioTek Synergy HI) to record the absorbance at 650 nm ...
-
No products found
because this supplier's products are not listed.
Mukundan Attur, et al.,
bioRxiv - Cell Biology 2019
Quote:
... supplemented with 10% FCS (Atlanta Biologicals). Cells at 50-70% confluency were transfected by overnight incubation with the indicated cDNAs in complete growth medium using TransIT®-LT1 - Transfection Reagent according to the manufacturer’s instructions (Mirus Bio ...
-
No products found
because this supplier's products are not listed.
Satyaveni Malasala, et al.,
bioRxiv - Biochemistry 2024
Quote:
... and His-DBS (DH/PH domain) were purchased from Cytoskeleton, Inc ...
-
No products found
because this supplier's products are not listed.
Niko Schwenzer, et al.,
bioRxiv - Cell Biology 2024
Quote:
HEK293 cells with constitutive expression of CaV subunits β3 and α2δ1 and inducible expression of α1D (Charles River Laboratories CT6232) were cultured in DMEM/F12 medium containing selection antibiotics and 0.6 µM isradipine (Sigma I6658) ...
-
No products found
because this supplier's products are not listed.
Tara A. Gleeson, et al.,
bioRxiv - Immunology 2024
Quote:
... mouse-anti-mouse NLRP3 (1/1000 dilution; Cryo2, Adipogen, AG-20B-0014), rabbit-anti-mouse gasdermin-D (1/1000 dilution ...
-
No products found
because this supplier's products are not listed.
Mitsugu Shimobayashi, et al.,
bioRxiv - Physiology 2020
Quote:
Plasma Leptin levels were measured by mouse mouse Leptin ELISA kit (Crystal Chem) according to the manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Sabira Mohammed, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... mouse HMGB1(high mobility group protein B1) ELISA Kit from Elabscience (Houston, TX). The optical density readings obtained were used to generate a four-parameter logistic curve and the concentration of the analytes in the samples were calculated by comparing to the standard curve generated.
-
No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... 5 μg of His-tagged H2A:H2B or His-H3:Flag-H4 (Diagenode) and 2 μg of Flag-hSpt16 were added to 250 μl of binding buffer (50 mM Tris-HCl pH 7.4 ...
-
No products found
Kirandeep K. Deol, et al.,
bioRxiv - Biochemistry 2020
Quote:
HEK293 Expressing Rpn11-HTBH (Applied Biological Materials, T6007)
-
No products found
because this supplier's products are not listed.
Mirren Charnley, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Fc-DL4 (86 nM; BioVision, P1163), Fc-VCAM-1 (19 nM ...
-
No products found
because this supplier's products are not listed.
Amrita Sule, et al.,
bioRxiv - Cancer Biology 2021
Quote:
YFP-ATM was immunoprecipitated from stably transfected HEK293 cells by GFP-TRAP (Chromotek). The immunoprecipitates were suspended in kinase assay buffer containing 2 μCi of [γ-32P] ATP ...
-
No products found
because this supplier's products are not listed.
Erik Procko,
bioRxiv - Biochemistry 2020
Quote:
Hydrated anti-human IgG Fc biosensors (Molecular Devices) were dipped in expression medium containing RBD-IgG1 for 60 s ...
-
No products found
because this supplier's products are not listed.
Peng Zhang, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Keigi Fujiwara (University of Texas MD Anderson Cancer Center, Houston, TX) and IP3R1-null HEK293 cells (Kerafast, Inc., Boston, MA) (Alzayady ...
-
No products found
because this supplier's products are not listed.
Tamina Lebek, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... and resuspended in 10% FCS in PBS with either DAPI (1 μM; Biotium, #40043) or DRAQ7 (300 nM ...
-
No products found
because this supplier's products are not listed.
Nuno Apóstolo, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Mice were placed in a mouse stereotact (KOPF) equipped with a neonatal mouse adaptor (Stoelting). During the rest of the procedure 2.5% isoflurane was constantly administered ...
-
No products found
because this supplier's products are not listed.
Ryan J. Duchatel, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... A Mouse C-peptide ELISA (ALPCO), was then used to quantify C-peptide levels in the plasma ...