-
No products found
because this supplier's products are not listed.
Alexis Bouin, et al.,
bioRxiv - Microbiology 2022
Quote:
... Cells were incubated with anti-VP1 antibodies (Dako, anti-enterovirus clone5-D8/1) diluted 1:500 in blocking solution for detection of viral protein VP1 or anti dsRNA antibody (SCICONS ...
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No products found
because this supplier's products are not listed.
Hongbing Jiang, et al.,
bioRxiv - Microbiology 2019
Quote:
... enterovirus D68 Ab (GeneTex), Anti-HA (ThermoFisher) ...
-
No products found
because this supplier's products are not listed.
Janine Vetter, et al.,
bioRxiv - Microbiology 2023
Quote:
... or pcDNA-V5-VP1 and 72 µl of Lipofectamine 2000 (Invitrogen) following the manufacturer instructions ...
-
No products found
because this supplier's products are not listed.
Stephanie Jost, et al.,
bioRxiv - Immunology 2023
Quote:
JCPyV capsid protein VP1 was obtained from Abcam (AB74569). Endpoint titer dilution ELISA was performed to determine the JCPyV serostatus of all healthy donors included in our study as previously published [73] ...
-
No products found
because this supplier's products are not listed.
Sallieu Jalloh, et al.,
bioRxiv - Immunology 2022
Quote:
... anti-dsRNA (Pan-Enterovirus Reagent, clone 9D5, Light Diagnostics, Millipore) antibody was used 1:2 overnight and anti-mouse-AF488 (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Mareike D. Hoffmann, et al.,
bioRxiv - Biochemistry 2023
Quote:
... or only VP1 (anti-AAV VP1 mouse monoclonal, A, lyophilized, purified, Progen) was diluted 1:250 in 5 % skim milk solution in TBS-T and incubated overnight at 4 °C ...
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No products found
because this supplier's products are not listed.
Pranav N.M. Shah, et al.,
bioRxiv - Biophysics 2020
Quote:
... The anti-VP1 monoclonal antibody (one of two monoclonal antibodies in a pan-Enterovirus ELISA detection kit) was obtained from Quidel (Athens, Ohio).
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No products found
because this supplier's products are not listed.
Chihong Song, et al.,
bioRxiv - Pathology 2019
Quote:
... the cells were incubated with anti- MNV VP1 rabbit polyclonal antibody labeled by Dylight 488 (LNK221D488, BioRad) for 30 min at 4°C ...
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No products found
because this supplier's products are not listed.
Damon H. May, et al.,
bioRxiv - Immunology 2024
Quote:
... Parvovirus B19 VLP/VP1/VP2 Co-Capsid Recombinant protein was purchased from Raybiotech) were biotinylated at optimized biotin-to-protein ratios that generated biotinylated proteins with 1-3 biotin(s ...
-
No products found
because this supplier's products are not listed.
Hongxia Zhang, et al.,
bioRxiv - Microbiology 2020
Quote:
... VP1 and MAT1 genes were cloned into Vector pGBKT7 (Clontech, USA). We generated pGBKT7-VP1 and pGADT7-MAT1 plasmids to map the regions in VP1 that were required for their interaction in the yeast-two hybrid system ...
-
No products found
because this supplier's products are not listed.
Li Chen Cheah, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... VP1 was excised by NotI and NheI (NEB) digestion and patched with a single-stranded DNA oligonucleotide ...
-
No products found
because this supplier's products are not listed.
Prashant Kumar, et al.,
bioRxiv - Immunology 2023
Quote:
... VP1-3 proteins were then bound to the S-Trap column and digested with trypsin (Promega Corporation) for 2 hrs at 47°C ...
-
No products found
because this supplier's products are not listed.
Lili Zhang, Khashayar Shahin, Ran Wang,
bioRxiv - Microbiology 2021
Quote:
... followed by incubation with a rabbit polyclonal anti-VP1 antibody (Ma, et al., 2020) or anti-guanylate binding protein (GBP) 1–5 (Santa Cruz Biotechnology, Santa Cruz, CA, USA). After washing ...
-
No products found
because this supplier's products are not listed.
Li Chen Cheah, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... ΔVP1 was codon-optimised for yeast and synthesised by GenScript. All other synthetic genes were manually codon-optimised for S ...
-
No products found
because this supplier's products are not listed.
Zhetao Zheng, et al.,
bioRxiv - Bioengineering 2021
Quote:
... were coated with 100 mM bicarbonate/carbonate buffer (pH 9.6) containing 0.03 μg/mL of recombinant proteins (VP1) from homologous wild-type viruses (Sino Biological Inc., Beijing, China) overnight at 4 °C ...
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No products found
because this supplier's products are not listed.
Alexander G. Shaw, et al.,
bioRxiv - Microbiology 2020
Quote:
Amplified poliovirus VP1 products from nested PCR were purified using QIAquick PCR purification kit (Qiagen) and sequenced using an ABI Prism 3130 genetic analyser (Applied Biosystems ...
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No products found
because this supplier's products are not listed.
Akihiro Fujikawa, et al.,
bioRxiv - Biochemistry 2019
Quote:
Protein extracts were precleaned with Protein G Sepharose (GE Healthcare) and subjected to immunoprecipitation with a combination of anti-FLAG M2 and Protein G Sepharose beads ...
-
No products found
because this supplier's products are not listed.
L. Martin, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... F0425)/500 µg protein with protein A-agarose (Roche). Fished-out FGFR3 protein was eluted and then denatured at 95°C for 10 minutes in NuPAGE LDS sample buffer (Life Technologies ...
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No products found
because this supplier's products are not listed.
Madhulika Singh, Aravind Penmatsa, Dipankar Nandi,
bioRxiv - Microbiology 2023
Quote:
Purified proteins and native protein standard (Supelco-Merck) were loaded in 4-15% gradient mini protean precast native gel (Bio-Rad ...
-
No products found
because this supplier's products are not listed.
Dharendra Thapa, et al.,
bioRxiv - Physiology 2019
Quote:
... Immunocaptured proteins were isolated using Protein-G agarose beads (Cell Signaling Technology), washed multiple times with CHAPS buffer ...
-
No products found
because this supplier's products are not listed.
Elle A. Campbell, et al.,
bioRxiv - Microbiology 2019
Quote:
To visualise PBG98-VP1-GFP11 virus infection, DF1 cells were seeded onto coverslips (TAAB, UK) in 24-well plates (Falcon, Corning, UK) at a density of 1.6×105 per well and transfected with GFP1-10 using lipofectamine 2000 24 hours prior to infection with the PBG98-VP1-GFP11 virus ...
-
No products found
because this supplier's products are not listed.
Sanjib Saha, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Protein expression was normalized to total protein (DM-TP01, Protein Simple) and compared between cell types.
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No products found
because this supplier's products are not listed.
Tamara Weiss, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Protein extracts were stored in Protein LoBind tubes (Eppendorf) at −80°C ...
-
No products found
because this supplier's products are not listed.
Justin A. Peruzzi, et al.,
bioRxiv - Bioengineering 2023
Quote:
... purified FLAG tagged protein (recombinant p53 protein, R&D Systems) was run with vesicles to quantify loading ...
-
No products found
because this supplier's products are not listed.
Kylene M. Harold, et al.,
bioRxiv - Physiology 2023
Quote:
... Total protein was stained (Revert Total Protein Stain, LI-COR) and imaged (Odyssey CLx ...
-
No products found
because this supplier's products are not listed.
Rachel Baum, et al.,
bioRxiv - Cell Biology 2022
Quote:
A His-tagged mouse Capping Protein (F-actin-capping protein subunit alpha-1 and F-actin-capping protein subunit beta) plasmid was obtained from Addgene as described above (Plasmid #89950) ...
-
No products found
because this supplier's products are not listed.
Mohamed F. Hamed, et al.,
bioRxiv - Microbiology 2022
Quote:
... Housekeeping protein β-actin (a cytoskeleton protein; dilution, 1:5,000; BD Biosciences) was used as loading controls ...
-
No products found
because this supplier's products are not listed.
Matthew D. Lauver, et al.,
bioRxiv - Immunology 2022
Quote:
... Bound virus was detected with rabbit polyclonal VP1 antibody (1:10000) followed by an anti-rabbit secondary (Biolegend, 1:200). Samples were acquired on an LSRFortessa flow cytometer (BD Biosciences ...
-
No products found
because this supplier's products are not listed.
Marion Thépaut, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Proteins were detected using InstantBlue protein stain (Expedeon) according to the supplier’s instructions.
-
No products found
because this supplier's products are not listed.
May Daher, et al.,
bioRxiv - Immunology 2020
Quote:
... Cas9 protein (PNA bio) and gRNA were incubated at room temperature for 15 min in a 3:1 reaction with 15μg Cas9 and 5000 ng gRNA (2500ng of sgRNA#1 and 2500ng of sgRNA#2).47 The incubation product (gRNA and Cas9 complex ...
-
No products found
because this supplier's products are not listed.
Lalitha Yadhanapudi, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Proteins were labelled for 5 mins (for soluble proteins) or 15 mins (for membrane proteins) with 30 µCi of Express Protein Labelling Mix (Perkin Elmer) per sample at their restrictive temperature ...
-
No products found
because this supplier's products are not listed.
Qiuli Shi, et al.,
bioRxiv - Microbiology 2021
Quote:
Proteins were labeled using the Monolith NT Protein Labeling Kit RED (NanoTemper Technologies) according to the manufacturer’s protocol and using a buffer consisting of 20 mM HEPES ...
-
No products found
because this supplier's products are not listed.
Izumi Yanatori, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Equal amounts of extract protein were incubated with GFP-binding protein-conjugated agarose (Chromotek) or anti-RFP antibody (Anti-RFP RTU - Rockland Biosciences ...
-
No products found
because this supplier's products are not listed.
Julia Manzetti, et al.,
bioRxiv - Microbiology 2020
Quote:
... mouse monoclonal IgG1 anti-BKPyV Vp1 (MAB3204-M19, Abnova), polyclonal rabbit anti-SV40 Vp2/Vp3 (ab53983 ...
-
No products found
because this supplier's products are not listed.
Alexandra I. Wells, et al.,
bioRxiv - Microbiology 2020
Quote:
The following antibodies were used-anti-VP1 (NCL-ENTERO, clone 5-D8/1, Leica Biosystems) and cleaved caspase 3 (Asp175 ...
-
No products found
because this supplier's products are not listed.
Michał A. Surma, et al.,
bioRxiv - Biochemistry 2021
Quote:
... healthy diets differing by protein content: Teklad global 14% protein (Envigo #2014S, low protein) and Teklad global 18% protein (Envigo #2018S ...
-
No products found
because this supplier's products are not listed.
Wei-Siang Liau, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Protein identification was carried out using Protein Pilot 5.0 (SCIEX) for database searching.
-
No products found
because this supplier's products are not listed.
Muaz Nik Rushdi, et al.,
bioRxiv - Immunology 2022
Quote:
... The purified proteins were biotinylated using biotin protein ligase (Avidity); excess biotin and ligase were removed with a Superdex 200 column (GE Healthcare) ...
-
No products found
because this supplier's products are not listed.
Katarzyna Bogucka-Janczi, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Cdc42 and ARP3 proteins or ARP2/3 protein complex (Cytoskeleton) were used as bait and full-length GST-ERK3 (SignalChem ...
-
No products found
because this supplier's products are not listed.
Stefanie Krug, et al.,
bioRxiv - Immunology 2021
Quote:
... Protein concentrations were determined by CB-X protein assay (G-Biosciences) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Qiuling Wang, Suiwen Hou,
bioRxiv - Plant Biology 2022
Quote:
... Protein concentration was determined using BCA protein assay kit (Solarbio, PC0021).
-
No products found
because this supplier's products are not listed.
Eli Isael Maciel, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Protein concentrations were determined using a Bradford protein assay (VWR, E530-1L). In a 1.5mL tube ...
-
No products found
because this supplier's products are not listed.
Stefan Kol, et al.,
bioRxiv - Bioengineering 2019
Quote:
... protein A biosensors (Pall) were equilibrated in sample diluent buffer (PBS containing 0.1% BSA and 0.02% Tween-20) ...
-
No products found
because this supplier's products are not listed.
Alexandra C. Walls, et al.,
bioRxiv - Immunology 2020
Quote:
... Protein A biosensors (FortéBio/Sartorius) were first hydrated for 10 minutes in Kinetics buffer ...
-
No products found
because this supplier's products are not listed.
David Gonzalez-Perez, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Proteins were separated by SDS-PAGE (12% Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) and transferred to PVDF membranes using an iBlot2 Gel Transfer Device (Thermo Fisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Lorna Cryan, et al.,
bioRxiv - Biochemistry 2019
Quote:
... matrix protein (Rockland Immunochemicals ...
-
No products found
because this supplier's products are not listed.
How-Wing Leung, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Total protein was isolated using an RNA-protein extraction kit (Macherey-Nagel), as specified by the manufacturer ...
-
No products found
because this supplier's products are not listed.
Hui Chen, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... The proteins were first purified using CaptivA Protein A affinity resin (Repligen), unless otherwise specified ...
-
No products found
because this supplier's products are not listed.
Pei Xuan Lee, et al.,
bioRxiv - Microbiology 2020
Quote:
... or OVA protein (InvivoGen) was administered iv to the infected mice.
-
No products found
because this supplier's products are not listed.
Yarui Zhang, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... and CDK6 proteins (Origene; 0.2 μg), respectively ...