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No products found
because this supplier's products are not listed.
Ann Logan, et al.,
bioRxiv - Immunology 2022
Quote:
... after which cells were permeabilised (0.1% Triton X100) and stained with an antibody recognising dengue virus envelope protein (Invitrogen MA1-27093, for DENV serotypes 1-3), pan flavivirus envelope (Millipore MAB10216 ...
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No products found
because this supplier's products are not listed.
Duyen Huynh, et al.,
bioRxiv - Biophysics 2024
Quote:
... and the virus envelope plasmid pMD2.G (Addgene #12259) using JetPRIME (Polyplus-transfection ...
-
No products found
because this supplier's products are not listed.
Michele Martins, et al.,
bioRxiv - Biochemistry 2020
Quote:
... it was screened for Dengue virus (DENV) using the Dengue NS1 Ag STRIP (Bio-Rad). The use of human blood was approved by the Fiocruz Ethical Committee (CAAE 53419815.9.0000.5248).
-
No products found
because this supplier's products are not listed.
Maria Vittoria Mancini, et al.,
bioRxiv - Microbiology 2020
Quote:
... Primary antibody for DENV was MAB8705 Anti-dengue virus complex antibody (Millipore); secondary antibody was the Goat anti-mouse Alexa Fluor 488 ...
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No products found
because this supplier's products are not listed.
Minyue Qiu, et al.,
bioRxiv - Microbiology 2022
Quote:
... sections were incubated with anti-dengue virus 1+2+3+4 primary antibodies (ab26837, Abcam, England) at 4°C overnight ...
-
No products found
because this supplier's products are not listed.
Juliet O. Obi, et al.,
bioRxiv - Biophysics 2024
Quote:
The first 70 nucleotides corresponding to SLA from the dengue virus serotype 2 genome (New Guinea C strain, GenBank no AF038403.1) was synthesized by GenScript in non-biotin and biotin-tagged forms ...
-
No products found
because this supplier's products are not listed.
Wei Zhang, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Adeno-associated virus (AAV) cell-lysates were produced using the AAVpro Purification Kit (All Serotypes) (Takara) with slight modifications ...
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No products found
because this supplier's products are not listed.
Jasmine Moshiri, et al.,
bioRxiv - Microbiology 2020
Quote:
... dengue protein NS2B/3/4A constructs were expressed in rabbit reticulocyte lysate (TNT coupled T7, Promega Bio Systems) programmed with 1 µg DNA/50 µL and labeled with 20 µCi of L-[35S]-methionine (EasyTag ...
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No products found
because this supplier's products are not listed.
René Platzer, et al.,
bioRxiv - Immunology 2023
Quote:
... coli serotype O55:B5 (Merck) for 24 to 48 hours to arrive at a mature BMDC culture or alternatively frozen to -80°C in 90% FCS (Biowest ...
-
No products found
because this supplier's products are not listed.
Michael J. Ricciardi, et al.,
bioRxiv - Immunology 2022
Quote:
... To sequence the envelope from serum virus we isolated viral RNA (Qiagen), synthesized cDNA ...
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No products found
because this supplier's products are not listed.
Toufic Kassouf, et al.,
bioRxiv - Cell Biology 2023
Quote:
... with virus packaging and envelope expressing plasmids using Fugene-6 (Roche). Medium was replaced with DMEM with 10% FCS the next day and supernatant was harvested 3 days post-transfection ...
-
No products found
because this supplier's products are not listed.
Déborah Lécuyer, et al.,
bioRxiv - Microbiology 2023
Quote:
... Envelope defective virus was pseudotyped with HU-1 SARS-CoV-2 Spike protein (pLV-Spike, Invivogen) and used for single round infection assays ...
-
No products found
because this supplier's products are not listed.
Carolyn Marquis, et al.,
bioRxiv - Cell Biology 2020
Quote:
... The time between nuclear envelope breakdown (NEB) and anaphase onset (AO ...
-
No products found
because this supplier's products are not listed.
Bolutife Fakoya, et al.,
bioRxiv - Microbiology 2021
Quote:
... cholerae serotype specific antisera (BD Difco) was performed using glass slides to confirm serotypes ...
-
No products found
because this supplier's products are not listed.
Fabiana Sassone, et al.,
bioRxiv - Pathology 2023
Quote:
... encoding the AAV serotype 8 capsid and pHelper (Agilent) encoding the AV helper functions ...
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No products found
because this supplier's products are not listed.
Matthias Höllerhage, et al.,
bioRxiv - Neuroscience 2024
Quote:
Adenoviral vectors of serotype 5 (BioFocus, Charles River Laboratories Nederland B.V. ...
-
No products found
because this supplier's products are not listed.
Qiwang Xiang, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... SARS-CoV-2 Envelope protein (Cell Signaling Technology, 74698S); furin (Proteintech ...
-
No products found
because this supplier's products are not listed.
Sofya A. Polyanskaya, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... and the envelope protein expressing plasmid (VSV-G) using polyethylenimine (PEI 25K™; Polysciences; Cat. No. 23966) transfection reagent ...
-
No products found
because this supplier's products are not listed.
Daniel Perez-Zsolt, et al.,
bioRxiv - Microbiology 2021
Quote:
... Virus particles were immunostained with rabbit anti-SARS-CoV-2 N protein (Sino Biological) or rabbit anti-GM1 Ab (Abcam ...
-
No products found
because this supplier's products are not listed.
David Brena, et al.,
bioRxiv - Developmental Biology 2020
Quote:
GST fusion proteins were generated using pGEX5x.3 (GE Healthcare) as vector ...
-
No products found
because this supplier's products are not listed.
Kyung-Tae Lee, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and 10 μg virus envelope plasmid (VSV-G) using PEIPro DNA transfection reagent (#115010, VWR, Radnor, PA, USA). GFP-α-arrestins lentiviral particles were harvested 40 hr-post transfections ...
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No products found
because this supplier's products are not listed.
Michelle A. Baird, et al.,
bioRxiv - Cell Biology 2023
Quote:
Cellular immunofluorescence for nuclear envelope proteins was performed using 1205Lu cells plated on cleaned coverslips (#1.5, 22 mm x 22 mm, Corning) for 24-72 hours depending on experimental conditions ...
-
No products found
because this supplier's products are not listed.
John P. Alao, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Myc (9E10) and pan 14-3-3 (K-19) proteins were from Santa Cruz Biotechnology (Heidelberg ...
-
No products found
because this supplier's products are not listed.
Jiejun Zhu, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 400nl of virus was delivered to 3 locations respectively by a microinjection system (Nanoliter 2010, WPI Ltd) through a craniotomy (<1 mm2) ...
-
No products found
because this supplier's products are not listed.
Peiyuan Chai, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... Siglec-3 Fc chimera protein (R&D Systems), Siglec-4 Fc chimera protein (R&D Systems) ...
-
No products found
because this supplier's products are not listed.
Chen-xi Zhang, et al.,
bioRxiv - Immunology 2020
Quote:
The serum levels of a total of 12 virus-related cytokines were measured by a bead-based immunoassay panel (Mouse Anti-Virus Panel, Cat No: 740622, Biolegend, USA). The BALF levels of a total of 12 inflammatory cytokines were measured by a bead-based immunoassay panel (Mouse Inflammation Panel ...
-
No products found
because this supplier's products are not listed.
Vanessa M. Doulames, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Rho-associated protein kinase inhibitor (3 uM; 1293823; PeproTech) was added to the media for 1 day post replating.
-
No products found
because this supplier's products are not listed.
Maria L. Bonatelli, et al.,
bioRxiv - Microbiology 2022
Quote:
... Proteins were removed by ultrafiltration (3 kDa Vivaspin; Sartorius, Göttingen, Germany). ACE and ANSA hydrolysis activities were quantified in discontinuous HPLC-based assays at 30°C in lysis buffer ...
-
No products found
because this supplier's products are not listed.
Michael B. Fessler, et al.,
bioRxiv - Immunology 2022
Quote:
... The virus sequence was confirmed by Illumina sequencing and did not differ from the parental virus ...
-
No products found
because this supplier's products are not listed.
Jamie C. Moffa, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Virus was removed from the upper chamber of the concentrator and stored in low protein binding tubes (Eppendorf #022431064) at -80°C.
-
No products found
because this supplier's products are not listed.
Jonathan Burnie, et al.,
bioRxiv - Microbiology 2021
Quote:
The quantification of HIV-1 p24 capsid protein was performed in captured virus lysates and virus-containing supernatants with the high-sensitivity AlphaLISA p24 detection kit following the manufacturer’s (PerkinElmer) instructions ...
-
No products found
because this supplier's products are not listed.
Jason Allen, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Virus was concentrated using ultracentrifugation (95000 g for 3 hr) and suspended using OPTI-MEM (Lonza). Viral transfections assays were performed by plating target 293T (ACE2+ve ...
-
No products found
because this supplier's products are not listed.
Juan Manuel García-Arcos, et al.,
bioRxiv - Biophysics 2022
Quote:
... 20 μM AACOCF3 inhibiting the nuclear envelope stretchsensitive enzyme cPLA2 (Tocris Bioscience #1462), 100 μM 2-APB blocking stretch-activated inositol triphosphate receptors (InsP3Rs ...
-
No products found
because this supplier's products are not listed.
Yusa Akiba, et al.,
bioRxiv - Microbiology 2024
Quote:
The virus solutions were centrifuged at 106800 × g for 3 hours at 4 °C using a SW32Ti rotor (Beckman Coulter, Fullerton, CA, USA). The virus pellets were suspended in phosphate-buffered saline (PBS) ...
-
No products found
because this supplier's products are not listed.
Brooke K. Hayes, et al.,
bioRxiv - Microbiology 2023
Quote:
... 3 μL of 3 mg/mL of protein was added to glow discharged UltrAuFoil holey gold grid (Quantifoil GmbH) using a Vitrobot Mark IV (Thermofisher ...
-
No products found
because this supplier's products are not listed.
Kyoko Tossell, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Stereotaxic virus injections were performed using an Angle Two apparatus (Leica) linked to a digital brain atlas (Leica Biosystems Richmond ...
-
No products found
because this supplier's products are not listed.
Dipti Rai, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 2-3 μl of protein was applied to glow-discharged carbon-coated copper grids (EMS; CF-400-Cu) and incubated for 45 s at room temperature ...
-
No products found
because this supplier's products are not listed.
Joshua J. Sims, et al.,
bioRxiv - Genetics 2021
Quote:
... We measured reporter virus transduction activity on a luminometer (BioTek) using the Renilla Glo Kit (Promega ...
-
No products found
because this supplier's products are not listed.
Annie M Goettemoeller, et al.,
bioRxiv - Neuroscience 2024
Quote:
... the membranes were washed 3 times and biotinylated proteins were detected on Odyssey Infrared Imaging System (LI-COR Biosciences). In parallel ...
-
No products found
because this supplier's products are not listed.
Varun Tiwari, et al.,
bioRxiv - Biophysics 2020
Quote:
Purified GLIC protein was reconstituted into liposomes formed with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (PE, Avanti Polar Lipids) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1’-rac-glycerol ...
-
No products found
because this supplier's products are not listed.
Judit Vágó, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Equal amounts of protein (3 µg) were loaded into 12–230 kDa separation modules (Protein Simple, Bio-Techne ...
-
No products found
because this supplier's products are not listed.
Farès Ousalem, et al.,
bioRxiv - Microbiology 2024
Quote:
Protein samples were injected onto a Yarra 3 µm SEC-2000 (Phenomenex) running at RT in 150 mM NaCl ...
-
No products found
because this supplier's products are not listed.
Deike J. Omnus, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... The final protein sample was concentrated (Pall Advance Centrifugal Device, MWCO 3 kDa), adjusted to 100 µM and stored in aliquots at −80°C ...
-
No products found
because this supplier's products are not listed.
Timothy P. Sheahan, et al.,
bioRxiv - Microbiology 2020
Quote:
... nanoluciferase expression as a surrogate for virus replication was quantitated on a Spectramax (Molecular Devices) plate reader according to the manufacturer’s instructions (Promega ...
-
No products found
because this supplier's products are not listed.
Ana Lima, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... and 3 μM GSK-3 inhibitor CHIR9902 (Cayman Chemicals) for 2 days at 37°C in 5% CO2 incubator ...
-
No products found
because this supplier's products are not listed.
Romina Marone, et al.,
bioRxiv - Bioengineering 2023
Quote:
... containing 300 cells and with 10ng/ml IL-3 (+IL-3 treatment) or without IL-3 (-IL-3 treatment) were plated in a well of a SmartDish (StemCell Technologies, Seattle, WA, USA) in duplicates ...
-
No products found
because this supplier's products are not listed.
Casey L. Mahoney-Crane, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Sections were developed using ImmPACT-3 3’-diaminobenzidine (DAB; Vector Labs), sequentially dehydrated as previously described (Stoyka et al. ...
-
No products found
because this supplier's products are not listed.
Jingyi Guo Fuglstad, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Fluorescent signal from the virus was amplified by immunostaining against Red Fluorescent Protein (RFP) (catalog no.5F8, Chromotek GmbH, Germany), followed by secondary antibody-staining with Alexa 546-tagged Goat Anti-rat IgG (catalog no ...
-
No products found
because this supplier's products are not listed.
John K. Mich, et al.,
bioRxiv - Neuroscience 2023
Quote:
Virus injection and optic fiber implantation surgery was performed in C57BL/6J mice (The Jackson Laboratory, #000664) at around P60 ...
-
No products found
because this supplier's products are not listed.
Vidur Garg, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... + 3% donkey serum (Jackson ImmunoResearch)] at room temperature for 15 mins ...