-
No products found
because this supplier's products are not listed.
Rachel J. Harding, et al.,
bioRxiv - Biochemistry 2019
Quote:
... HTT protein was eluted with 1 cell paste volume of buffer supplemented with 250 μg/mL 3xFLAG peptide (Chempep) run twice over the anti-FLAG resin ...
-
No products found
because this supplier's products are not listed.
Sheng Xiao, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Virus was injected using a manual volume displacement injector (Narishige International USA, MMO-220A) connected to a glass pipette (Drummond Scientific ...
-
No products found
because this supplier's products are not listed.
Eli Fritz McDonald, et al.,
bioRxiv - Cell Biology 2021
Quote:
... purified from B lymphocyte hybridoma cells using a Recombinant Protein G - Sepharose 4B Affinity Column on an ÄKTA start protein purification system (GE Life Science Product # 29022094). Antibodies were used in immunoblotting buffer (5% bovine serum albumin (BSA ...
-
No products found
because this supplier's products are not listed.
Amy R. Rappaport, et al.,
bioRxiv - Immunology 2021
Quote:
... The sequence of a E1 (578-3404 bp)/E3 deleted virus (2,125-31,825 bp) was assembled into pUC19 from VR-594-derived and synthetic (SGI-DNA) fragments ...
-
No products found
because this supplier's products are not listed.
Heather Swann, et al.,
bioRxiv - Biophysics 2020
Quote:
... CA) along with 1:1000 dilution of Anti-Membrane Protein (2019-nCoV) Polyclonal Antibody (NCV-M-005, eEnzyme, Gaithersburg, MD) and then immunoprobed with appropriate infrared secondary antibody ...
-
No products found
because this supplier's products are not listed.
Sarah C. Donnelly, et al.,
bioRxiv - Microbiology 2022
Quote:
... samples containing 1–3 mg/mL of protein were evaluated using ICP-MS (Biotron Analytical Services, Western University, London, Canada). Briefly ...
-
No products found
because this supplier's products are not listed.
Ayan Rajgarhia, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... and protein extraction was carried out using a total protein extraction Kit (BioChain Institute, Inc. Hayward, CA). Protein concentration was evaluated using the Modified Lowry Protein Assay (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Lihe Su, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... was cloned into mammalian cell expression vectors and transiently transfected into Expi293 cells (1 liter scale, Bon Opus Biosciences) or CHO cells (3 liter scale ...
-
No products found
because this supplier's products are not listed.
Petra Pfenninger, Laura Yerly, Jun Abe,
bioRxiv - Immunology 2021
Quote:
... cells were restimulated with 1 µM SIINFEKL (BAP-201, EMC Microcollections, Tübingen, Germany) and 5 µg/mL brefeldin A (B6542 ...
-
No products found
because this supplier's products are not listed.
Rebecca Kochanowsky, et al.,
bioRxiv - Microbiology 2022
Quote:
... Anti-Myc antibody (Protein Mods, Madison WI, USA) was used at 1:5,000 and the detecting anti-mouse antibody (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Andrew D. Weems, et al.,
bioRxiv - Cell Biology 2022
Quote:
VitroGel coffins were prepared by embedding MV3 or A375 cells in VitroGel 3D or RGD at 1:1 dilution according to manufacturer’s instructions (TheWell Biosciences, sku # TWG001). Briefly ...
-
No products found
because this supplier's products are not listed.
Kara Poole, et al.,
bioRxiv - Bioengineering 2024
Quote:
... Fixed cells were blocked with 1% bovine serum albumin fraction V (Equitech-Bio, Kerrville, TX) in PBS for 1 hour at room temperature ...
-
No products found
because this supplier's products are not listed.
Kai Sha, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Human cell lines were incubated with antibodies against CD166-FITC (3A6; Ancell, Stillwater, MN; 1:1250) and CD49f-Alexa Fluor® 647 (GoH3 ...
-
No products found
because this supplier's products are not listed.
Valerio Marino, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Protein photocrosslinking was performed by irradiating the cells with 365-nm UV for 15 minutes under a UV lamp (UVITEC LF-215.LS LAMP 365/254NM 2X15W 230V).
-
No products found
because this supplier's products are not listed.
Daniel García-Ruano, et al.,
bioRxiv - Cell Biology 2021
Quote:
Cell length at division was determined from Blankophor images (1 mg/mL Blankophor solution, A108741, Ambeed Inc.) using FIJI (National Institutes of Health ...
-
No products found
because this supplier's products are not listed.
Oh Sung Kwon, et al.,
bioRxiv - Cell Biology 2020
Quote:
... To repress protein synthesis 100 μg/ml of cycloheximide (TOKU-E) and 300 μM of puromycin (InVivoGen ...
-
No products found
because this supplier's products are not listed.
Abishek Chandrashekar, et al.,
bioRxiv - Microbiology 2022
Quote:
... or B.1.1.529 (Omicron; GISAID ID: EPI_ISL_7358094.2) Spike proteins (21st Century Biochemicals). 106 peripheral blood mononuclear cells well were re-suspended in 100 µL of R10 media supplemented with CD49d monoclonal antibody (1 µg/mL ...
-
No products found
because this supplier's products are not listed.
Robert S. Kellar, et al.,
bioRxiv - Bioengineering 2020
Quote:
... The proteins were dissolved into 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP, Oakwood Chemical, Estill, SC). The biomimetic WHDs were prepared using a ratio of 9:1 collagen to rhTE ...
-
No products found
because this supplier's products are not listed.
Devika Prasanth, et al.,
bioRxiv - Biophysics 2019
Quote:
Simulated microgravity conditions were produced using a Rotary Cell Culture System (RCCS™) (Fig. 1) developed at the Johnson Space Center by NASA and made commercially available by Synthecon® Inc (Houston ...
-
No products found
because this supplier's products are not listed.
Maia Kinnebrew, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Dhcr7-/- and Dhcr24-/- NIH/3T3 cells were cultured for 1 week prior to experiments in supplemented DMEM containing 5% Lipoprotein-Depleted Serum (LDS) (Kalen Biomedical, LLC). To induce ciliation and test for SHH responsiveness ...
-
No products found
because this supplier's products are not listed.
Elva Vidya, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... Two mg of proteins were used in four rounds of rabbit injection (Capralogics, Cambridge Massachusetts) and a total of three bleeds were collected and characterized.
-
No products found
because this supplier's products are not listed.
Elodie Billey, et al.,
bioRxiv - Plant Biology 2020
Quote:
... Antibodies against the LARP6C protein were custom made (at Agro-Bio, La Ferté St Aubin, France) through immunization of rabbits with peptide “KRTSQFTDRDREELGQ” (amino acids 220-235) ...
-
No products found
because this supplier's products are not listed.
Aniuska Becerra-Artiles, et al.,
bioRxiv - Immunology 2022
Quote:
... and MHC proteins were removed using a Vydac C18 microspin column (The Nest Group, Ipswich, MA). The mixture of MHC and peptides were bound to the column ...
-
No products found
because this supplier's products are not listed.
Machmouchi Dana, et al.,
bioRxiv - Microbiology 2024
Quote:
... ZIKV infectivity was assessed using the mouse anti-E protein mAb 4G2 (RD-Biotech, Besançon, France). Antibody donkey anti-mouse Alexa Fluor 488 IgG (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Anton Petcherski, et al.,
bioRxiv - Bioengineering 2023
Quote:
... were diluted 1:500 – 1:1000 times in tris buffer electrolyte (IZON Sciences) prior to running samples according to manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Krissie Tellez, et al.,
bioRxiv - Physiology 2019
Quote:
... Plasma GLP-1 levels were quantified with an active GLP-1 ELISA (Eagle Biosciences). Plasma amino acid levels were determined using the L-Amino Acid Quantitation Kit (Sigma Aldrich ...
-
No products found
because this supplier's products are not listed.
Jennifer B. Phillips, et al.,
bioRxiv - Neuroscience 2023
Quote:
... α-PCDH15: 1:200 (NSJ Bioreagents); Alexafluor Goat anti-rabbit 488 or 568 (Thermo Fisher).
-
No products found
because this supplier's products are not listed.
Miguel Alejandro Lopez-Ramirez, et al.,
bioRxiv - Biochemistry 2019
Quote:
... 2-hydroxy-1-naphthaldehyde (Ark Pharm); and 2-amino-N-(1-phenylethyl)benzamide (Enamine).
-
No products found
because this supplier's products are not listed.
Enikő Lázár, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... 1 U/µl RiboProtect (BLIRT, RT35), 1.0 U/µl T4 Rnl2 (NEB ...
-
No products found
because this supplier's products are not listed.
Takefumi Kimura, et al.,
bioRxiv - Molecular Biology 2023
Quote:
LX-2 cells were obtained from Sigma-Aldrich and HepG2 cells were procured from Cellular Engineering Technologies ...
-
No products found
because this supplier's products are not listed.
Rahul Sharma, Martin W. Hetzer,
bioRxiv - Cell Biology 2022
Quote:
... mouse Nesprin3 (Nordic-MUBio # MUB1317P,1:250); rabbit Emerin D3B9G XP (CST # 30853) ...
-
No products found
because this supplier's products are not listed.
Hyeong-Min Lee, et al.,
bioRxiv - Cancer Biology 2023
Quote:
Non-homologous end joining (NHEJ) in BE2C and GIMEN neuroblastoma cell lines was visualized by using a custom cell-based kit for NHEJ (Topogen, Cat # DR5000A). Following the manufacturer’s guide ...
-
No products found
because this supplier's products are not listed.
Katherine L. Leiby, et al.,
bioRxiv - Bioengineering 2022
Quote:
Primary rat lung microvascular endothelial cells (RLMVEC, VEC Technologies) were cultured on fibronectin (1 μg/cm2 ...
-
No products found
because this supplier's products are not listed.
Stephen D. Carter, et al.,
bioRxiv - Cell Biology 2021
Quote:
... The bead suspension was made by a 1:1 dilution of 500 nm blue (345/435 nm) polystyrene fluorospheres (Phosphorex) with a 3:1 concentrated solution of 20 nm:5 nm colloidal gold (Sigma Aldrich ...
-
No products found
because this supplier's products are not listed.
Frank M. Mason, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... telomere (PNA, TelC-A647, 1:500), Acro-P (Cytocell, LPE NOR, undiluted) or rDNA (Empire Genomics, RPCI23-225M6, 1:5) FISH probes were diluted in hybridization solution (10mM Tris-HCl pH7.2 ...
-
No products found
because this supplier's products are not listed.
Sean K. Wang, Yunlu Xue, Constance L. Cepko,
bioRxiv - Neuroscience 2021
Quote:
... or 1:500 of anti-SIRPα (QED Bioscience) in blocking solution overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
J.I. Hoare, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... All cell lines underwent 16 locus STR verification (DNA Diagnostics Centre ...
-
No products found
because this supplier's products are not listed.
Jared R. Bagley, et al.,
bioRxiv - Animal Behavior and Cognition 2021
Quote:
... The back wall of the box is attached to the mouse cage (7 1/2” × 11 1/2” × 5”, N10 Polycarbonate Mouse Cage, Ancare, NY USA) via thumbscrews or hinges attached with thumb screws for the servo access-control version ...
-
No products found
because this supplier's products are not listed.
Rebeca San Martin, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... These cell lines were grown in HPC1 media (Athena Enzyme Systems) supplemented with 10% FBS ...
-
No products found
because this supplier's products are not listed.
Jakub Gemperle, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... and cells imaged in 1x Opti-Klear medium (Marker Gene Technologies Inc) supplemented with 10% (v/v ...
-
No products found
because this supplier's products are not listed.
Jorge Antolio Domínguez-Bautista, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... 2% bovine serum albumin suitable for cell culture (RMBIO BSA-BAF-25G), and antibiotic/antimycotic (Life Technologies ...
-
No products found
because this supplier's products are not listed.
Sophie E. Cousineau, et al.,
bioRxiv - Microbiology 2022
Quote:
... mouse anti-HCV core (clone B2, Anogen MO-I40015B, 1:7,500); mouse anti-JFH-1 NS5A (clone 7B5 ...
-
No products found
because this supplier's products are not listed.
Amanda Morgan, et al.,
bioRxiv - Neuroscience 2022
Quote:
... DAGL inhibitor DO34 (50 mg kg-1, Glixx Laboratories Inc., MA) was administered in a vehicle mixture of ethanol (Pharmco ...
-
No products found
because this supplier's products are not listed.
Helen J. von Richthofen, et al.,
bioRxiv - Immunology 2022
Quote:
... Cells were treated 2h at 37°C with neutrophil elastase (Elastin Products Company), cathepsin G (Biocentrum) ...
-
No products found
because this supplier's products are not listed.
Jun Yu Ip, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... with absorbance at 254 nm monitored using a Triax Flow Cell (Biocomp Instruments). Monosome fractions were pooled and treated with 200 μg/mL Proteinase K (Roche ...
-
No products found
because this supplier's products are not listed.
Lewis D. Evans, et al.,
bioRxiv - Neuroscience 2020
Quote:
Genomic DNA was extracted from cell pellets using QuickExtract DNA Extraction Solution (Cambio; QE09050) as per the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Rocío del M. Saavedra-Peña, et al.,
bioRxiv - Physiology 2022
Quote:
... Cells were then stained with anti-BrdU antibody (Alexa Fluor 647; Phoenix Flow Systems; AX647) at 1:30 in HBSS with 3% BSA overnight in the dark at 4C ...
-
No products found
because this supplier's products are not listed.
Tongcui Ma, et al.,
bioRxiv - Immunology 2021
Quote:
... Cells were then washed three times with CyFACS buffer and stained with Maleimide DOTA (Macrocyclics) for 30 min on ice ...
-
No products found
because this supplier's products are not listed.
Alina Sigaeva, et al.,
bioRxiv - Cell Biology 2023
Quote:
Human colon adenocarcinoma HT-29 cells were routinely cultured in Dulbecco’s Modified Eagle’s Medium (Cellutron Life Technologies, USA) with a high glucose concentration ...
-
No products found
because this supplier's products are not listed.
Michael Rale, et al.,
bioRxiv - Biochemistry 2022
Quote:
... we melted a powder mix of 9 parts HO-PEG-NH2 and 1 part biotin-CONH-PEG-NH2 by weight (cat. #103000-20 and #133000-25-20, Rapp Polymere, Tübingen, Germany) at 75°C ...