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Chikungunya Virus E2 Envelope Protein HEK293 Human Fc Tag
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Sino Biological
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... of the spike protein (S-RBD) of the SARS-CoV-2 virus (with human Fc as a tag for detection) was purchased from Sino Biological (Beijing). -
Thermo Fisher
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019Quote: ... 2.4 µM human His6-UBE2T E2 enzyme (Fisher Scientific, E2-695), 5 mM adenosine triphosphate ... -
R&D Systems
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2020, published in Journal of Proteome Research doi: 10.1021/acs.jproteome.0c00521Quote: ... with C-terminal 6xHis tag produced in HEK293 cells and human recombinant PD-L1-Fc chimera protein produced in mouse NS0 cells were obtained from R&D Systems, Minneapolis ... -
Addgene
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2021Quote: ... Vesicular Stomatitis Virus glycoprotein (VSV-G) envelope expression vector (pMD2.G; Addgene #12259), lentiviral packaging plasmid (psPax2 ... -
Millipore Sigma
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018Quote: ... monoclonal antibodies (mAbs) against VEEV envelope glycoproteins E1 (3B2A9) (22) or E2 (MAB 8767, EMD Millipore, Burlington, MA, USA) were used ... -
Becton, Dickinson and Company
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... Human Fc Block (BD) was added to all surface staining cocktails ... -
BioLegend
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019Quote: ... and vesicular stomatitis virus (VSV) tag (Biolegend UK Ltd, London, UK) followed by the appropriate secondary peroxidase-conjugated antibody (Sigma ... -
GenScript
No products found because this supplier's products are not listed.Cited in RGEN-SEQ FOR HIGHLY SENSITIVE AMPLIFICATION-FREE SCREEN OF OFF-TARGET SITES OF GENE EDITORSbioRxiv - Genomics 2021Quote: ... human Hek293 DNA was purchased from Genscript. S ... -
abcam
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... using a primary mouse monoclonal antibody against the YFV envelope protein (Abcam) and a secondary Alexa488 fluor-conjugated goat anti-mouse IgG antibody (Molecular Probes) ... -
Jackson ImmunoResearch
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: RE goat-anti-human IgG-Fc (Jackson Immunoresearch); PE anti-human HLA-A,B,C (clone W6/32 ... -
Genetex
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... Then cells were labeled with the monoclonal anti-CHIKV E2 envelope glycoprotein clone CHK-48 made in mouse (BEI Resources, Manassas, VA, USA) and the polyclonal anti-Zika virus envelope protein made in rabbit (GeneTex, Irvine, CA, USA). The antibodies used for immunofluorescence included Alexa Fluor 488 conjugated goat anti-rabbit IgG (Invitrogen ... -
Takara Bio
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2020Quote: Human Embryonic Kidney 293 (HEK293) cells (Clontech Laboratories) were used for most experiments described in this study ... -
Acrobiosystems
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2020Quote: ... human ACE2 (Fc-tag) were all purchased from Acrobiosystems (S1N-C52H3 ... -
GE Life Sciences
No products found because this supplier's products are not listed.Cited in Effect of SARS-CoV-2 spike mutations on animal ACE2 usage and in vitro neutralization sensitivitybioRxiv - Microbiology 2021Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ... -
Boston Biochem
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2020Quote: ... recombinant human UbcH5a/UBE2D1 (E2-616, Boston Biochem), Myc-ubiquitin (U-115 ... -
Bethyl
No products found because this supplier's products are not listed.Cited in Functional Enrichment and Analysis of Antigen-Specific Memory B Cell Antibody Repertoires in PBMCsbioRxiv - Immunology 2019, published in Frontiers in Immunology doi: 10.3389/fimmu.2019.01452Quote: ... anti-human IgA Fc or anti-human IgM Fc (Bethyl Labs, Montgomery, TX) at 2μg/ml in PBS ... -
Promega
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018Quote: ... and pMD.G (vesicular stomatitis virus glycoprotein [VSV-G] envelope plasmid) [53] at a ratio of 1:1:0.5 using FuGENE 6 (Promega) according to the manufacturer’s protocol ... -
American Type Culture Collection
No products found because this supplier's products are not listed.bioRxiv - Systems Biology 2020Quote: HEK293 (human, DMSZ, ACC 635) and U2OS (human, American Type Culture Collection [ATCC], HTB-96) cell were cultivated in DMEM (Gibco ... -
Qiagen
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2021Quote: ... and Fc-tag ACE2 protein was purified using a protein affinity A column (Qiagen). Proteins were further purified by gel filtration (Superdex™ 200 Increase 10/30GL ... -
New England Biolabs
No products found because this supplier's products are not listed.Cited in Fascetto Interacting Protein (FIP) Regulates Fascetto (PRC1) to Ensure Proper Cytokinesis and PloidybioRxiv - Cell Biology 2018Quote: Nuclear envelope breakdown (NEB) to anaphase timing was determined based on a sudden change in the nuclear outline and the first detectable separation of sister chromatids respectively ... -
SouthernBiotech
No products found because this supplier's products are not listed.bioRxiv - Immunology 2018, published in Journal of Virology doi: 10.1128/jvi.01495-18Quote: ... alkaline phosphatase conjugated anti-human IgG Fc antibody (Southern Biotech) at 1:2,000 dilution in 1×PBS was added after 4 times washing ... -
Cell Signaling Technology
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... the 96 well plate was coated with human ACE2 recombinant protein (1μg/ml, mFc tag; Cell Signaling Technology). The plate was blocked with PBS containing 5% skim milk for 2 h at RT ... -
Cayman Chemical
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019Quote: ... Exogenous prostaglandin E2 (Cayman Chemicals) and 15-keto prostaglandin E2 (Cayman Chemicals ... -
Invivogen
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... Anti-human IgG Fc Capture (AHC) Biosensors tips were initially loaded with Dectin-1A:FC fusion protein (Invivogen, #fc-hdec1a) at 13 ug/ml ... -
Origene Technologies
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019, published in Archives of Biochemistry and Biophysics doi: 10.1016/j.abb.2019.108166Quote: ... protein tag or peptide tag (Myc-DDK; OriGene Technologies). Images of the mCherry-tagged transfected cells were taken 24 hours posttransfection ... -
Merck
No products found because this supplier's products are not listed.Cited in Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancerbioRxiv - Cancer Biology 2021Quote: ... 17β-estradiol (E2; Merck KGaA) was added 48 hours later to a final concentration of 10 nM (an equal volume of ethanol was added as vehicle control ... -
Tocris
No products found because this supplier's products are not listed.bioRxiv - Immunology 2018Quote: ... Prostaglandin E2 (PGE2) from Tocris Bioscience and (±)-Propranolol hydrochloride from Sigma-Aldrich. -
Peprotech
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019, published in Stem Cell Reports doi: 10.1016/j.stemcr.2019.12.007Quote: ... 2 ng ml−1 recombinant human TGFβ1 (112 amino acid, HEK293-derived, Peprotech, 100-21). Cells were routinely maintained in E8 medium on 1:800 diluted growth factor reduced Matrigel (see below) ... -
LI-COR
No products found because this supplier's products are not listed.Cited in Multiple Capsid Protein Binding Sites Mediate Selective Packaging of the Alphavirus Genomic RNAbioRxiv - Microbiology 2020Quote: ... E2 signal was imaged and quantified using the Odyssey Fc Imaging System (LI-COR Biosciences) to determine particle number ... -
Miltenyi Biotec
No products found because this supplier's products are not listed.Cited in Reporters of TCR signaling identify arthritogenic T cells in murine and human autoimmune arthritisbioRxiv - Immunology 2019, published in Proceedings of the National Academy of Sciences doi: 10.1073/pnas.1904271116Quote: ... Human Fc blocking reagent (Miltenyi Biotec) and 2.4G2 mouse blocking reagent were used in all human and mouse stains respectively. -
Roche
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2021Quote: ... The His6-tag fusion proteins were purified with a cOmplete™ His-tag purification resin (Roche, Merck). The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich ... -
RayBiotech
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... Human recombinant ACE2-Fc-tag (Raybiotech) was then added at 1 μg/mL and incubated overnight at 4 °C ... -
Agilent
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... human embryonic kidney cells 293 (HEK293; Agilent #240073) were calcium phosphate-transfected with the recombinant AAV2 plasmid and a 3-helper system ... -
Mirus Bio
No products found because this supplier's products are not listed.bioRxiv - Systems Biology 2021Quote: ... torque teno virus hypothetical protein or human respirovirus 3 D protein were transfected into the cells using TransIT-LT1 (Mirus Bio) according to manufacturer’s protocol ... -
Lonza
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: HEK293 cells (Lonza) were cultured in DMEM (Lonza ... -
Corning
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2021Quote: ... Human HEK293 cells were maintained in DMEM (Corning) supplemented with 10% FBS (Gibco) ... -
Bio-Rad
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2020Quote: ... goat anti-human IgG Fc (Biorad; 5211-8004; 1:2000) and rabbit anti-Vinculin (EPR8185 ... -
Santa Cruz
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2020Quote: ... anti-E2 (Santa Cruz Biotechnology, sc-271534), anti-E3 (Santa Cruz Biotechnology ... -
Polysciences
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2021Quote: ... and the envelope protein expressing plasmid (VSV-G) using polyethylenimine (PEI 25K™; Polysciences; Cat. No. 23966) transfection reagent ... -
Novus Biologicals
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... rabbit polyclonal antibody to WNV envelope protein (1:800, Novus Biologicals #NB100-56744), and rabbit polyclonal antibody to Iba-1 (1:200 ... -
Illumina
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2020, published in Nature doi: 10.1038/s41586-020-2536-xQuote: ... cDNA libraries were then tagmented using the Illumina/Nextera DNA prep kit (FC 121-1030) with index tags from Illumina (FC 121-1031), cleaned up with Ampure XP beads ... -
BPS Bioscience
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... an RBD-Fc fragment (319-541) with a C-terminal human Fc-tag was purchased from BPS Bioscience (San Diego, CA). Recombinant biotinylated ACE2 was purchased from Acro Biosystems (Newark ... -
Alpha Diagnostic
No products found because this supplier's products are not listed.Cited in Integrated technology platform for accelerated discovery of antiviral antibody therapeuticsbioRxiv - Immunology 2020Quote: A modified protocol using a commercially available human anti-zika virus Envelope protein (ZIKV-Env) IgG kit (Alpha Diagnostics International) was used to quantify ZIKV-117 mAb levels in NHP serum samples ... -
NanoTemper
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2021Quote: ... Recombinant human FTO protein was labeled with His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies GmbH; MO-L008). The labelled FTO protein (target ... -
GeneCopoeia
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2020Quote: ... lentivirus particles were made using HEK293 cells (ATCC) transfected with 2nd generation packaging/envelope plasmids (Dr. Yasuhiro Ikeda, Mayo Clinic) and shRNA clones (GeneCopoeia). U251 cells were obtained from American Type Cell Culture and maintained for in vitro experiments in DMEM supplemented with 10% FBS and 1% Antibiotic Antimycotic Solution ... -
Proteintech
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2022Quote: ... Purified HAX1 protein (Proteintech, Ag27244, fused with His-tag) was labeled with RED-NHS 2nd Generation dye according to the supplied labeling protocol Monolith NT™ Protein Labeling Kit ... -
PerkinElmer
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018, published in BMC Biology doi: 10.1186/s12915-018-0503-xQuote: ... 35S-labelled cysteine and methionine Easy Tag Express protein labelling mix (Perkin Elmer) were added to cells for the final 10 minutes of the time course before addition of 20 µg/ml cycloheximide and incubation on ice ... -
Mabtech
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 S2 N defined peptide pool contains 41 synthetic peptides binding to human HLA derived from the S and N proteins of the SARS-CoV-2 virus (#3620-1, Mabtech AB)58. -
MyBioSource
No products found because this supplier's products are not listed.Cited in The combination of Bromelain and Acetylcysteine (BromAc) synergistically inactivates SARS-CoV-2bioRxiv - Microbiology 2020Quote: ... The recombinant Envelope protein was obtained from MyBioSource (Cat#MBS8309649). All other reagents were from Sigma Aldrich. -
Greiner
No products found because this supplier's products are not listed.bioRxiv - Immunology 2022Quote: ... Anti-Human Fc biosensors (ForteBio, catalog #18-0015) were submerged in wells of black 96-well microplates (Greiner, catalog #655209) containing 250 μL of kinetics buffer (PBS ...