-
No products found
because this supplier's products are not listed.
Ferdinando M. Milazzo, et al.,
bioRxiv - Microbiology 2022
Quote:
... 1.0 μg/mL human ACE2 protein mouse Fc-tag (Sino Biological) was added and incubated for 1 h at 37 °C ...
-
No products found
because this supplier's products are not listed.
Renjing Wang, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... 2.4 µM human His6-UBE2T E2 enzyme (Fisher Scientific, E2-695), 5 mM adenosine triphosphate ...
-
No products found
because this supplier's products are not listed.
Julius Benicky, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... with C-terminal 6xHis tag produced in HEK293 cells and human recombinant PD-L1-Fc chimera protein produced in mouse NS0 cells were obtained from R&D Systems, Minneapolis ...
-
No products found
because this supplier's products are not listed.
Duyen Huynh, et al.,
bioRxiv - Biophysics 2023
Quote:
... and the virus envelope plasmid pMD2.G (Addgene #12259) using JetPRIME (Polyplus-transfection ...
-
No products found
because this supplier's products are not listed.
Silvia Kocanova, et al.,
bioRxiv - Cell Biology 2023
Quote:
... cells were grown for 3 days in phenol red-free media supplemented with 10% charcoal-stripped FCS (-E2) and subsequently treated with 100nM E2 (Sigma) for the indicated times.
-
No products found
because this supplier's products are not listed.
N Ogino, et al.,
bioRxiv - Cell Biology 2022
Quote:
... recombinant proteins Serpin E2 (BioLegend, 769002) and Serpin A3 (R&D systems ...
-
No products found
because this supplier's products are not listed.
Emily Speranza, et al.,
bioRxiv - Immunology 2021
Quote:
... Human Fc Block (BD) was added to all surface staining cocktails ...
-
No products found
because this supplier's products are not listed.
Apurva T. Prabhakar, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... E2 tag [5E11] 1:1000 (Abcam, catalog no. ab977).
-
No products found
because this supplier's products are not listed.
P.K Smitha, et al.,
bioRxiv - Molecular Biology 2022
Quote:
IgGFc: Human IgG Fc Sequence (Supplementary Material Figure S1) was codon optimized for HEK293 expression and synthesized from Genscript USA in pUC57 vector ...
-
No products found
because this supplier's products are not listed.
Chao Gao, et al.,
bioRxiv - Biochemistry 2020
Quote:
... human ACE2 (Fc-tag) were all purchased from Acrobiosystems (S1N-C52H3 ...
-
No products found
because this supplier's products are not listed.
Sarah A. Shelby, Thomas R. Shaw, Sarah L. Veatch,
bioRxiv - Biophysics 2022
Quote:
... VCAM-1/Fc chimeric proteins and anti-human Fc antibodies (recombinant human VCAM-1/Fc chimera protein, Fisher 862-VC-100; Fcγ-specific goat anti-human IgG, Jackson Immunoresearch 109-005-008) or poly-L-lysine (Sigma P4707).
-
No products found
because this supplier's products are not listed.
Ayijiang Yisimayi, et al.,
bioRxiv - Immunology 2023
Quote:
Human ACE2 with Fc tag was immobilized onto Protein A sensor chips using a Biacore 8K (GE Healthcare). Purified SARS-CoV-2 mutants RBD were prepared in serial dilutions ...
-
No products found
because this supplier's products are not listed.
Jianing Song, et al.,
bioRxiv - Biochemistry 2020
Quote:
... recombinant human UbcH5a/UBE2D1 (E2-616, Boston Biochem), Myc-ubiquitin (U-115 ...
-
No products found
because this supplier's products are not listed.
Anny Gravdal, et al.,
bioRxiv - Cell Biology 2020
Quote:
Human Embryonic Kidney 293 (HEK293) cells (Clontech Laboratories) were used for most experiments described in this study ...
-
No products found
because this supplier's products are not listed.
Déborah Lécuyer, et al.,
bioRxiv - Microbiology 2023
Quote:
... Envelope defective virus was pseudotyped with HU-1 SARS-CoV-2 Spike protein (pLV-Spike, Invivogen) and used for single round infection assays ...
-
No products found
because this supplier's products are not listed.
Magdalena Oroń, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Prostaglandin E2 10nM (Tocris) was added only to medium for normal tissue organoids.
-
No products found
because this supplier's products are not listed.
George N. Llewellyn, et al.,
bioRxiv - Microbiology 2023
Quote:
... mouse-anti-human IgG Fc-HRP (Southern Biotech) was diluted 1:2000 in blocking buffer and added and plates were incubated for 2 hours at 370C ...
-
No products found
because this supplier's products are not listed.
Fang Tian, et al.,
bioRxiv - Biophysics 2021
Quote:
... and Fc-tag ACE2 protein was purified using a protein affinity A column (Qiagen). Proteins were further purified by gel filtration (Superdex™ 200 Increase 10/30GL ...
-
No products found
because this supplier's products are not listed.
Yun-Fei Ma, et al.,
bioRxiv - Immunology 2024
Quote:
... FC tags on the S and RBD proteins were removed through treatment with Factor Xa Proteinase (Promega).
-
No products found
because this supplier's products are not listed.
Marco Brustolin, et al.,
bioRxiv - Microbiology 2021
Quote:
... Then cells were labeled with the monoclonal anti-CHIKV E2 envelope glycoprotein clone CHK-48 made in mouse (BEI Resources, Manassas, VA, USA) and the polyclonal anti-Zika virus envelope protein made in rabbit (GeneTex, Irvine, CA, USA). The antibodies used for immunofluorescence included Alexa Fluor 488 conjugated goat anti-rabbit IgG (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Eric Waltari, et al.,
bioRxiv - Immunology 2019
Quote:
... anti-human IgA Fc or anti-human IgM Fc (Bethyl Labs, Montgomery, TX) at 2μg/ml in PBS ...
-
No products found
because this supplier's products are not listed.
Fynn M. Hansen, et al.,
bioRxiv - Systems Biology 2020
Quote:
HEK293 (human, DMSZ, ACC 635) and U2OS (human, American Type Culture Collection [ATCC], HTB-96) cell were cultivated in DMEM (Gibco ...
-
No products found
because this supplier's products are not listed.
Toufic Kassouf, et al.,
bioRxiv - Cell Biology 2023
Quote:
... with virus packaging and envelope expressing plasmids using Fugene-6 (Roche). Medium was replaced with DMEM with 10% FCS the next day and supernatant was harvested 3 days post-transfection ...
-
No products found
because this supplier's products are not listed.
Irina Buckle, et al.,
bioRxiv - Immunology 2022
Quote:
... human IL-12p70 (HEK293) (Peprotech) and 50ng/ml recombinant human IL-18 (MBL International ...
-
No products found
because this supplier's products are not listed.
Amy Lewis, Philip Michael Elks,
bioRxiv - Immunology 2019
Quote:
... Exogenous prostaglandin E2 (Cayman Chemicals) and 15-keto prostaglandin E2 (Cayman Chemicals ...
-
No products found
because this supplier's products are not listed.
Vanessa B. Sanchez, Saima Ali, Math P. Cuajungco,
bioRxiv - Cell Biology 2019
Quote:
... protein tag or peptide tag (Myc-DDK; OriGene Technologies). Images of the mCherry-tagged transfected cells were taken 24 hours posttransfection ...
-
No products found
because this supplier's products are not listed.
Tomoyuki Nishikawa, et al.,
bioRxiv - Immunology 2021
Quote:
... 96-well plates were coated with human ACE2 recombinant protein (1μg/ml, mFc tag; #83986, Cell Signaling Technology), and then blocked using PBS containing 5% skim milk for 2 h at room temperature ...
-
No products found
because this supplier's products are not listed.
Judith F. Ashouri, et al.,
bioRxiv - Immunology 2019
Quote:
... Human Fc blocking reagent (Miltenyi Biotec) and 2.4G2 mouse blocking reagent were used in all human and mouse stains respectively.
-
No products found
because this supplier's products are not listed.
Rinshi S. Kasai, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Human β2AR was fused at its N-terminus to the ACP- or Halo7-tag protein (NEB and Promega, respectively) at the cDNA level ...
-
No products found
because this supplier's products are not listed.
Rebecca S. Brown, et al.,
bioRxiv - Microbiology 2020
Quote:
... E2 signal was imaged and quantified using the Odyssey Fc Imaging System (LI-COR Biosciences) to determine particle number ...
-
No products found
because this supplier's products are not listed.
Wei-Li Ling, Samuel Ken-En Gan,
bioRxiv - Immunology 2022
Quote:
KD measurements of Fc-tagged Human FcμR (Cat: 13556-H02H, SinoBiological) immobilised on Anti-Human IgG Fc (AHC) (Cat: 18-5060, Sartorius) biosensors were performed on the Octet Red96® system with the loading threshold set at 1.0 nm and utilizing the recombinant Pertuzumab and Trastuzumab IgM VH whole antibodies variants in five serial diluted concentrations (12.5 to 200nM ...
-
No products found
because this supplier's products are not listed.
Natalia Vydra, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... 17β-estradiol (E2; Merck KGaA) was added 48 hours later to a final concentration of 10 nM (an equal volume of ethanol was added as vehicle control ...
-
No products found
because this supplier's products are not listed.
Yadira M. Soto-Feliciano, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Human HEK293 cells were maintained in DMEM (Corning) supplemented with 10% FBS (Gibco) ...
-
No products found
because this supplier's products are not listed.
Awadalkareem Adam, et al.,
bioRxiv - Immunology 2021
Quote:
... Human recombinant ACE2-Fc-tag (Raybiotech) was then added at 1 μg/mL and incubated overnight at 4 °C ...
-
No products found
because this supplier's products are not listed.
Tai L. Ng, et al.,
bioRxiv - Systems Biology 2021
Quote:
... torque teno virus hypothetical protein or human respirovirus 3 D protein were transfected into the cells using TransIT-LT1 (Mirus Bio) according to manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Jérôme Wahis, et al.,
bioRxiv - Neuroscience 2020
Quote:
... human embryonic kidney cells 293 (HEK293; Agilent #240073) were calcium phosphate-transfected with the recombinant AAV2 plasmid and a 3-helper system ...
-
No products found
because this supplier's products are not listed.
Jingwei Zeng, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... goat anti-human IgG Fc (Biorad; 5211-8004; 1:2000) and rabbit anti-Vinculin (EPR8185 ...
-
No products found
because this supplier's products are not listed.
Jaehyoun Lee, et al.,
bioRxiv - Biochemistry 2020
Quote:
... anti-E2 (Santa Cruz Biotechnology, sc-271534), anti-E3 (Santa Cruz Biotechnology ...
-
No products found
because this supplier's products are not listed.
Masaya Yamaguchi, et al.,
bioRxiv - Microbiology 2019
Quote:
Human TLR2/NF-κB/SEAP stably transfected HEK293 cells and human TLR4/MD-2/CD14/NF-κB/SEAP stably transfected HEK293 cells (Novus Biologicals, Centennial ...
-
No products found
because this supplier's products are not listed.
Laura Alonso-Herranz, et al.,
bioRxiv - Immunology 2020
Quote:
HEK293 cells (Lonza) were cultured in DMEM (Lonza ...
-
No products found
because this supplier's products are not listed.
Simona Selberg, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Recombinant human FTO protein was labeled with His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies GmbH; MO-L008). The labelled FTO protein (target ...
-
No products found
because this supplier's products are not listed.
Sofya A. Polyanskaya, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... and the envelope protein expressing plasmid (VSV-G) using polyethylenimine (PEI 25K™; Polysciences; Cat. No. 23966) transfection reagent ...
-
No products found
because this supplier's products are not listed.
Gary Baisa, et al.,
bioRxiv - Immunology 2020
Quote:
... an RBD-Fc fragment (319-541) with a C-terminal human Fc-tag was purchased from BPS Bioscience (San Diego, CA). Recombinant biotinylated ACE2 was purchased from Acro Biosystems (Newark ...
-
No products found
because this supplier's products are not listed.
Peng He, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... cDNA libraries were then tagmented using the Illumina/Nextera DNA prep kit (FC 121-1030) with index tags from Illumina (FC 121-1031), cleaned up with Ampure XP beads ...
-
No products found
because this supplier's products are not listed.
Derek D.C. Ireland, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Primary antibodies used include: human polyclonal anti-Zika virus antibody (Kerafast EVU302, MA) or mouse anti-ZikV E protein mAb (Fitzgerald ...
-
No products found
because this supplier's products are not listed.
Anna Balcerak, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Purified HAX1 protein (Proteintech, Ag27244, fused with His-tag) was labeled with RED-NHS 2nd Generation dye according to the supplied labeling protocol Monolith NT™ Protein Labeling Kit ...
-
No products found
because this supplier's products are not listed.
Renée M. van der Sluis, et al.,
bioRxiv - Immunology 2021
Quote:
... the SARS-CoV-2 envelope (E) protein (ABclonal RP01263) or the SARS-CoV-2 spike (S ...
-
No products found
because this supplier's products are not listed.
Kyung-Tae Lee, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and 10 μg virus envelope plasmid (VSV-G) using PEIPro DNA transfection reagent (#115010, VWR, Radnor, PA, USA). GFP-α-arrestins lentiviral particles were harvested 40 hr-post transfections ...
-
No products found
because this supplier's products are not listed.
Jack M Shireman, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... lentivirus particles were made using HEK293 cells (ATCC) transfected with 2nd generation packaging/envelope plasmids (Dr. Yasuhiro Ikeda, Mayo Clinic) and shRNA clones (GeneCopoeia). U251 cells were obtained from American Type Cell Culture and maintained for in vitro experiments in DMEM supplemented with 10% FBS and 1% Antibiotic Antimycotic Solution ...
-
No products found
because this supplier's products are not listed.
Javed Akhter, et al.,
bioRxiv - Microbiology 2020
Quote:
... The recombinant Envelope protein was obtained from MyBioSource (Cat#MBS8309649). All other reagents were from Sigma Aldrich.