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No products found
because this supplier's products are not listed.
Seraphine Kamayirese, et al.,
bioRxiv - Biochemistry 2023
Quote:
(His)6-14-3-3ε was commercially obtained from Novus Biologicals (Centennial CO, USA), and 14-3-3ε-(His)12 was expressed in our laboratory (see details on protein expression in the supporting information) ...
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No products found
because this supplier's products are not listed.
Maryna V. Ivanchenko, et al.,
bioRxiv - Neuroscience 2023
Quote:
Total RNA was extracted from transduced HEK 293 cells using the Zymo Quick-RNA Microprep Kit (Zymo Research, #R1050). Reverse transcription was performed with Invitrogen’s SuperScript IV VILO Master Mix with ezDNAse enzyme (Invitrogen ...
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No products found
because this supplier's products are not listed.
Marina Vortmeyer-Krause, et al.,
bioRxiv - Cell Biology 2020
Quote:
... with the respective lamin containing or empty (Mock) vectors into HEK 293-TN cells using PureFection nanotechnology-based transfection reagent (System Biosciences). The medium was exchanged the next day and replaced by antibiotics-free DMEM containing 10% FCS ...
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No products found
because this supplier's products are not listed.
Federico Bertoglio, et al.,
bioRxiv - Biochemistry 2020
Quote:
... S1-His or RBD-His were labelled using Monolith NTTM His-Tag Labeling Kit RED-tris-NTA (Nanotemper) according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Evan R. Buechel, Heather W. Pinkett,
bioRxiv - Biochemistry 2023
Quote:
... pAG-MNase (EpiCypher 14-1048) and 100mM CaCl2 was added to the immobilized cells and incubated for 2h at 4°C to digest chromatin DNA ...
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No products found
because this supplier's products are not listed.
Isabel Soto, et al.,
bioRxiv - Neuroscience 2024
Quote:
... and KO (n=14; (Envigo)) rats ...
-
No products found
because this supplier's products are not listed.
Gihoon Lee, Tom W. Muir,
bioRxiv - Biochemistry 2023
Quote:
... Doxycycline (Cas No.24390-14-5) from Stem Cell Technologies, and SGK1 inhibitor GS-9007 (Cas No.1426214-51-8 ...
-
No products found
because this supplier's products are not listed.
Alessio D. Nahmad, et al.,
bioRxiv - Immunology 2020
Quote:
... or anti-mouse IgG or anti-mouse IgG1 or anti-mouse IgM (Jackson ImmunoResearch) at 2μg/ml in PBST and were incubated for two hours ...
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No products found
because this supplier's products are not listed.
Rebekah C. Gullberg, Judith Frydman,
bioRxiv - Molecular Biology 2022
Quote:
... HEK-293 cells were maintained in 10% fetal bovine serum (Atlanta Biologicals) in Dulbecco’s modified Eagle’s medium (DMEM ...
-
No products found
because this supplier's products are not listed.
Cary R. Boyd-Shiwarski, et al.,
bioRxiv - Cell Biology 2022
Quote:
... The homology arms were amplified from extracted HEK-293 cell genomic DNA (QuickExtract DNA extraction solution, Lucigen). The fidelity of all constructs was confirmed by Sanger sequencing prior to use.
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No products found
because this supplier's products are not listed.
Magda Krakowczyk, et al.,
bioRxiv - Molecular Biology 2023
Quote:
HEK-293 cells (60%-70% of confluency) were fixed by 2.5% glutaraldehyde and 2% paraformaldehyde (both from Electron Microscopy Science, Inc.) solution for 1 h at 4°C ...
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No products found
because this supplier's products are not listed.
Cheng-Tsung Pan, et al.,
bioRxiv - Evolutionary Biology 2022
Quote:
... A mouse 6X His tag antibody (HRP) (GeneTex Inc.) was then used to recognize Gal3-His at room temperature for 1 hour ...
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No products found
because this supplier's products are not listed.
Kerstin Voelkl, et al.,
bioRxiv - Neuroscience 2023
Quote:
... mouse anti-His (Dianova, DIA-900-100, 1:1,000), chicken anti-EGFP (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Ao Dong, et al.,
bioRxiv - Neuroscience 2020
Quote:
... His (Tocris), Epi (Sigma-Aldrich) ...
-
No products found
because this supplier's products are not listed.
Brian L. Hie, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
-
No products found
because this supplier's products are not listed.
Vishant Boradia, et al.,
bioRxiv - Microbiology 2022
Quote:
... Blots were probed with mouse α-His antibody followed by IRDye® 680RD Goat α-Mouse IgG Secondary Antibody (LI-COR) to detect the His tagged PE15 ...
-
No products found
because this supplier's products are not listed.
Carole Seguin-Devaux, et al.,
bioRxiv - Immunology 2024
Quote:
... or rabbit anti-His (Bethyl, ImTec Diagnostic NV ...
-
No products found
because this supplier's products are not listed.
Ge Song, et al.,
bioRxiv - Immunology 2020
Quote:
... Proteins without His tags were purified with GNL columns (Vector Labs). The bound proteins were washed with PBS and then eluted with 50 mL of 1M Methyl α-D-mannopyranoside (Sigma M6882-500G ...
-
No products found
because this supplier's products are not listed.
Mayuka Katagiri, et al.,
bioRxiv - Immunology 2024
Quote:
... PE anti-mouse CD62L (clone MEL-14, Tonbo Biosciences, 1:100), FITC anti-mouse CD86 (clone GL-1 ...
-
No products found
because this supplier's products are not listed.
Priyabrata Mohanty, et al.,
bioRxiv - Microbiology 2021
Quote:
... 50 μL mouse monoclonal anti-6X His tagged primary antibody (Calbiochem) was added at a dilution of 1:150 in 0.5 % BSA in PBST in the respective wells and incubated for 2 hours without shaking at room temperature ...
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No products found
because this supplier's products are not listed.
Xuanwen Li, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Recombinant mouse HEXB protein (His Tag) was obtained from MyBioSource (San Diego, CA). Recombinant proteins alcohol dehydrogenase (ADH ...
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No products found
because this supplier's products are not listed.
Carolin Fritz, et al.,
bioRxiv - Cell Biology 2024
Quote:
... His (MP Biomedicals) plates ...
-
No products found
because this supplier's products are not listed.
Jia Xuan Leong, et al.,
bioRxiv - Plant Biology 2021
Quote:
... Recombinant His-UBA1 and His-UBC8 were purified using Ni-Ted resin (Macherey-Nagel). Purified proteins were used for in vitro ubiquitination assays ...
-
No products found
because this supplier's products are not listed.
Rose Blake, et al.,
bioRxiv - Microbiology 2023
Quote:
... anti-His monoclonal antibody (Raybiotech) and goat anti-rabbit IgG were used as primary and secondary antibodies (Supplementary Table 1) ...
-
No products found
because this supplier's products are not listed.
Li-Ying Yu, et al.,
bioRxiv - Neuroscience 2023
Quote:
METTL3/14 complex (26342, Cayman Chemical) was labelled via His-tag with Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies GmbH) ...
-
No products found
because this supplier's products are not listed.
Julienne LaChance, Kevin Suh, Daniel J. Cohen,
bioRxiv - Systems Biology 2021
Quote:
... and a Qi2 sCMOS camera (Nikon Instruments, 14-bit). An automated XY stage ...
-
No products found
because this supplier's products are not listed.
Nikoline S. Olsen, et al.,
bioRxiv - Microbiology 2020
Quote:
Prepare two large (Ø 14 cm e.g. 82.1184.500, Sarstedt, Nürnbrecht DE or at least 12 × 8 cm e.g ...
-
No products found
because this supplier's products are not listed.
Olaf Klingbeil, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... MARK3 and 14-3-3ε were purchased (Active Motif, 81209 ...
-
No products found
because this supplier's products are not listed.
Pablo Alcón, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... 20 µM His-tagged ubiquitin (Enzo Life Sciences) in a reaction buffer of 50 mM HEPES pH 7.5 ...
-
No products found
because this supplier's products are not listed.
Patrick Blatt, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Rabbit anti-His (1:000, Rockland Inc., 600-401-382), Mouse anti-Fibrillarin (1:25 ...
-
No products found
because this supplier's products are not listed.
Weisong Zhao, et al.,
bioRxiv - Biophysics 2022
Quote:
... equipped with an objective (100×/1.7 HI oil, APON, Olympus)and a sCMOS (Flash 4.0 V3 ...
-
No products found
because this supplier's products are not listed.
Hisako Kaneda, et al.,
bioRxiv - Biochemistry 2020
Quote:
... or anti-human caspase 14 antibody (H00023581-M01; Abnova, Taipei, Taiwan; 0.6 μg/ml PBS) at 4 °C for overnight ...
-
No products found
because this supplier's products are not listed.
Camila de Britto Pará de Aragão, et al.,
bioRxiv - Neuroscience 2020
Quote:
... mouse anti-mouse Gephyrin (1:1000, Synaptic Systems, catalog #147021), Neuroligin-1 (1:300 ...
-
No products found
because this supplier's products are not listed.
Raphael Fettig, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... NIH-3T3 fibroblasts and HEK-293 cells (all from ATCC, LGC Standards GmbH, Wesel, Germany) were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
-
No products found
because this supplier's products are not listed.
Inês Caldeira Brás, et al.,
bioRxiv - Neuroscience 2021
Quote:
Human embryonic kidney (HEK) 293 cells (ATTC, VA, USA) were maintained in DMEM medium (PAN Biotech, Aidenbach, Germany) supplemented with 10% FBS (Anprotec ...
-
No products found
because this supplier's products are not listed.
Maike Pohlers, et al.,
bioRxiv - Cancer Biology 2023
Quote:
For automated dual luciferase reporter assay 2-2.5 x 104 HEK 293 T cells were seeded per 96-well by the liquid handling system epMotion 5075 (Eppendorf, Hamburg, Germany). HEK 293 T cells were transfected with 50 ng/well reporter vector with or without 3′UTR and 200 ng/well pSG5 empty vector or pSG5-miR-142-5p expression plasmid ...
-
No products found
because this supplier's products are not listed.
Maxime Lorenzini, et al.,
bioRxiv - Physiology 2020
Quote:
Whole-cell NaV currents were recorded at room temperature from transiently transfected HEK-293 cells using an Axopatch 200A amplifier (Axon Instruments, Molecular Devices, San Jose, CA) 48 hours after transfection ...
-
No products found
because this supplier's products are not listed.
Mitchell G. Thompson, et al.,
bioRxiv - Microbiology 2023
Quote:
... Plants were maintained in Sunshine Mix #4 soil (Sungro) supplemented with Osmocote 14-14-14 fertilizer (ICL) at 5mL/L and agroinfiltrated 29 days after seed sowing.
-
No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... 5 μg of His-tagged H2A:H2B or His-H3:Flag-H4 (Diagenode) and 2 μg of Flag-hSpt16 were added to 250 μl of binding buffer (50 mM Tris-HCl pH 7.4 ...
-
No products found
because this supplier's products are not listed.
Luise Linsenmeier, et al.,
bioRxiv - Neuroscience 2021
Quote:
Murine neuroblastoma cells (N2a; ACC148, DSMZ Germany) and the embryonic mouse hippocampal cell line mHippoE-14 (CLU198; CELLutions Biosystems Inc.) were cultured at 37°C in an atmosphere of 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM ...
-
No products found
because this supplier's products are not listed.
James E. Dennis, Taylor Splawn, Thomas J. Kean,
bioRxiv - Cell Biology 2019
Quote:
... Pseudolentiviral particles were made in HEK-293Ta cells (GeneCopoeia) by co-transfection of psPAX2 ...
-
No products found
because this supplier's products are not listed.
Dong V Wang,
bioRxiv - Neuroscience 2020
Quote:
... A sugar pellet (14 mg; Bio-Serv) was delivered by a food dispenser (Med ...
-
A dialyzed, lyophilized powder.
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Su Yeon Kim, et al.,
bioRxiv - Neuroscience 2022
Quote:
... and incubated in HBSS containing papain (Worthington; 14 U/mL) and DNase I (100 μg/mL ...
-
No products found
because this supplier's products are not listed.
Clémentine Villeneuve, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... guinea-pig anti-Keratin 14 (Progen, GP-CK14; 1:300), rabbit anti-Collagen IV (Abcam ...
-
No products found
because this supplier's products are not listed.
Muhammad Rizwan, et al.,
bioRxiv - Bioengineering 2022
Quote:
... methyl cellulose dialysis membrane 12-14 kDa cut-off (Spectrum Laboratories), Jag1 (188-204 (Cedarlane Labs) ...
-
No products found
because this supplier's products are not listed.
Satyaveni Malasala, et al.,
bioRxiv - Biochemistry 2024
Quote:
... and His-DBS (DH/PH domain) were purchased from Cytoskeleton, Inc ...
-
No products found
because this supplier's products are not listed.
Constanza Feliziani, et al.,
bioRxiv - Cell Biology 2020
Quote:
Triple IP3R knockout human embryonic kidney cell line (TKO-HEK) from Kerafast (Boston, MA, USA; #EUR030) was grown in DMEM (#11995-065 ...
-
No products found
because this supplier's products are not listed.
Engin Demirdizen, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... n=14 for survival) were intracranially injected using a fixed stereotactic apparatus (Stoelting). Mice were weighed and injected with 5 mg/kg Carprofen subcutaneously ...
-
No products found
because this supplier's products are not listed.
Tara A. Gleeson, et al.,
bioRxiv - Immunology 2024
Quote:
... mouse-anti-mouse NLRP3 (1/1000 dilution; Cryo2, Adipogen, AG-20B-0014), rabbit-anti-mouse gasdermin-D (1/1000 dilution ...
-
No products found
because this supplier's products are not listed.
Mitsugu Shimobayashi, et al.,
bioRxiv - Physiology 2020
Quote:
Plasma Leptin levels were measured by mouse mouse Leptin ELISA kit (Crystal Chem) according to the manufacturer’s instructions.