-
No products found
because this supplier's products are not listed.
Doris Krauter, et al.,
bioRxiv - Neuroscience 2021
Quote:
... using a diamond knife (Histo HI 4317, Diatome). Afterwards sections were stained according to Gallyas52 and with Methylene blue/ Azur II for 1 min ...
-
No products found
because this supplier's products are not listed.
Julian Schöllkopf, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
N-terminally His-tagged Pasteurella multocida toxin (PMT) was coated at 10 µg/mL (500 ng/well ...
-
No products found
because this supplier's products are not listed.
Chafen Lu, et al.,
bioRxiv - Microbiology 2019
Quote:
... Secondary antibodies were rabbit polyclonal anti-His (Delta Biolabs), Horseradish peroxidase (HRP)-anti-rabbit and HRP-anti-mouse IgG (GE Healthcare ...
-
No products found
because this supplier's products are not listed.
Carina C D Joe, et al.,
bioRxiv - Bioengineering 2021
Quote:
Residual host-cell protein (HCP) was quantified using the HEK293 HCP ELISA kit (Cygnus Technologies) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Saejeong Park, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... HEK293 cells were washed with PBS and lysed in NP-40 lysis buffer (Boston Bioproducts) including protease and phosphatase inhibitor (Roche) ...
-
No products found
because this supplier's products are not listed.
Martina Oravcová, et al.,
bioRxiv - Cell Biology 2022
Quote:
Endogenous level of DNA damage in HEK293 cells was evaluated using the CometAssay kit (Trevigen) according the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Laura Sánchez-Caballero, et al.,
bioRxiv - Cell Biology 2019
Quote:
... HEK293 cells expressing inducible NDUFAF3-GFP were cultured in a Wilco dish (Intracel, Royston, UK), washed with phosphate-buffered saline (PBS) ...
-
No products found
because this supplier's products are not listed.
Hao-Ching Jiang, et al.,
bioRxiv - Animal Behavior and Cognition 2023
Quote:
... HEK293 cells were plated on 12 mm round German glass coverslips (Bellco Biotechnology, #1943-10012A) coated with poly-d-lysine hydrobromide (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Ye Qiu, et al.,
bioRxiv - Microbiology 2020
Quote:
... Mouse anti-His tag monoclonal antibody (Bioworld, USA, 1:5000) was used as the first antibody ...
-
No products found
because this supplier's products are not listed.
Julia A Alvarez, et al.,
bioRxiv - Microbiology 2024
Quote:
... 20% heat inactivated (HI) fetal bovine serum (FBS) (Omega Scientific), 1% penicillin-streptomycin (Life Technologies ...
-
No products found
because this supplier's products are not listed.
Ole A.W. Haabeth, et al.,
bioRxiv - Immunology 2021
Quote:
... 2 μg of the RBD-his mRNA or GFP mRNA (Trilink) was formulated in 6.6 μl of PBS pH 5.5 with 1.37 μl of 5mM CART and added to the cells ...
-
No products found
because this supplier's products are not listed.
Huang Zhu, Dan S. Kaufman,
bioRxiv - Immunology 2019
Quote:
... 15 % heat-inactivated human AB serum (Valley Biomedical, Catalog # HP1022 HI),1 % P/S ...
-
No products found
because this supplier's products are not listed.
Alexander J. Finney, et al.,
bioRxiv - Microbiology 2019
Quote:
... Nitrocellulose membranes were challenged with an anti-His-HRP antibody (Alpha Diagnostics) and a GeneGnome instrument (SynGene ...
-
No products found
because this supplier's products are not listed.
Andrew J. Stout, et al.,
bioRxiv - Bioengineering 2022
Quote:
... or Beefy-R (Hi-Def B8 supplemented with 0.4 mg/mL RPI). Beefy-9 and Beefy-R were prepared immediately before use ...
-
No products found
because this supplier's products are not listed.
Mariska S. Simpson, et al.,
bioRxiv - Cell Biology 2023
Quote:
... His-tagged proteins were affinity purified by binding to cobalt resin (GoldBio) equilibrated in 1X PBS ...
-
No products found
because this supplier's products are not listed.
Camden R. Bair, et al.,
bioRxiv - Microbiology 2021
Quote:
... supplemented with 8% heat-inactivated newborn calf serum (HI-NBCS, Rocky Mountain Biologicals), 1.5 g/ml Na2CO3 (Amresco) ...
-
No products found
because this supplier's products are not listed.
Wanda M. Figueroa-Cuilan, et al.,
bioRxiv - Microbiology 2022
Quote:
... Hi-FBS was achieved by placing the FBS (Atlas Biologicals, FP-0500-A) in a water bath at ∼56 °C for 30 minutes ...
-
No products found
because this supplier's products are not listed.
Sumona P. Dhara, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Reactions were quenched in heat inactivated fetal calf serum (HI-FCS; Gemini Bio-Products) in DMEM/F12 (Gibco ...
-
No products found
because this supplier's products are not listed.
Neil J Rzechorzek, et al.,
bioRxiv - Biochemistry 2020
Quote:
... HEK293-F cells were grown in 400 mL batches in 2 L non-baffled Erlenmeyer flasks (TriForest Enterprises Inc, #FPC2000S) to a cell density of ∼1×106 cells/mL.
-
No products found
because this supplier's products are not listed.
Maria Steene Eriksen, et al.,
bioRxiv - Biochemistry 2019
Quote:
Fluorescently tagged Arc and StrepII tagged Arc were coexpressed in HEK293 cells and purified using Strep-Tactin Sepharose (IBA Lifesciences). Formation of complexes between the indicated Arc constructs was analyzed by immunoblotting following SDS electrophoresis ...
-
No products found
because this supplier's products are not listed.
Justine Creff, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 200 µg for HEK293) were incubated with 3 µg of the indicated antibodies and 12 µL protein sepharose beads (IPA300, Repligen) at 4°C for 4 h ...
-
No products found
because this supplier's products are not listed.
Peng Zhang, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Keigi Fujiwara (University of Texas MD Anderson Cancer Center, Houston, TX) and IP3R1-null HEK293 cells (Kerafast, Inc., Boston, MA) (Alzayady ...
-
No products found
because this supplier's products are not listed.
Jordan A. Stinson, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... stable HEK293-F cell lines for each cytokine were prepared through cloning into the expression cassette of PiggyBac (System Biosciences) transposon vector ...
-
No products found
because this supplier's products are not listed.
Shanshan Lang, et al.,
bioRxiv - Bioengineering 2019
Quote:
... The recombinant extracellular domain of CD38 and PD-L1 was produced as a 6-His and AviTag™ fusion in HEK293 cells and purified by Ni-NTA followed by in vitro biotinylation by BirA biotin ligase (Avidity).
-
No products found
because this supplier's products are not listed.
Anne Rehkamp, et al.,
bioRxiv - Biochemistry 2020
Quote:
LC separation of peptides originating from HEK293 cell membranes was performed via selfpacked C18 columns (PicoFrits, 75 μm x 50 cm, 15 μm tip, New Objective, self-packed with ReproSil-Pur 120 C18-AQ ...
-
No products found
because this supplier's products are not listed.
Katrin Manske, et al.,
bioRxiv - Bioengineering 2023
Quote:
20,000 Human Embryonic Kidney cells (HEK293-CD19+) expressing the CD19 antigen were seeded on a 96-well E-plate (ACEA Biosciences) over night ...
-
No products found
because this supplier's products are not listed.
Ronan Shaughnessy, et al.,
bioRxiv - Cell Biology 2023
Quote:
... lentivi-ral particles were produced in the HEK293 FT packaging cells transfected with MiniBAR constructs and the Excelenti LTX Lentivirus Packaging mix (Oxford Genetics) using lipo-fectamine following manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Meenakshi Basu Shrivastava, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 500 ng human recombinant His-tagged ubiquitin-conjugating enzyme (E2) Ube2d3 (from BIOMOL International, #U0880), in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Elena Arutyunova, et al.,
bioRxiv - Biophysics 2021
Quote:
... The fusion protein was subsequently digested with His-tagged SUMO protease (McLab, South San Francisco, CA) at 4 °C for 1–2 h to remove the SUMO tag and the resulting cleavage mixture was then passed through Ni-NTA resin column ...
-
No products found
because this supplier's products are not listed.
J. Cody Herron, et al.,
bioRxiv - Systems Biology 2022
Quote:
... supplemented with 2% HI-FBS and 20 μM Rho kinase inhibitor Y-27632 (Hello Bio, HB2297) for 25 min of inhibition during frustrated phagocytosis ...
-
No products found
because this supplier's products are not listed.
Bjoern Traenkle, et al.,
bioRxiv - Immunology 2021
Quote:
... an alpaca (Vicugna pacos) was immunized with the purified extracellular domains of human CD4 (aa26-390) recombinantly produced in HEK293 cells (antibodies-online GmbH, Germany). After initial priming with 1 mg ...
-
No products found
because this supplier's products are not listed.
Aditi Deshpande, et al.,
bioRxiv - Microbiology 2022
Quote:
... library preparation and Hi-Seq 2 x150 paired-end sequencing were performed by GENEWIZ (New Jersey, USA).
-
No products found
because this supplier's products are not listed.
Nikhil J. Parekh, et al.,
bioRxiv - Immunology 2019
Quote:
IFN-β protein was detected using the mouse Hi-Sensitivity IFN-β ELISA kit (PBL Assay Science). CCL4 protein was detected using the mouse CCL4/MIP-1 beta Quantikine ELISA kit (R&D Systems) ...
-
No products found
because this supplier's products are not listed.
Anna K. Hundsdoerfer, et al.,
bioRxiv - Genomics 2023
Quote:
... euphorbiae (from one head each; the same individuals used for the PacBio genome and Hi-C sequencing) and neural tissue from the internal reference standard Acheta domesticus (female ...
-
No products found
because this supplier's products are not listed.
Mohd Farid Abdul Halim, et al.,
bioRxiv - Microbiology 2023
Quote:
... maripaludis MM1265 or Δfdh1 Δfdh2 expressing fdh2-His were grown in 1-liter anaerobic Schott bottles (Chemglass) containing McCas-formate medium with a N2:CO2 (80:20 ...
-
Talacotuzumab (Anti-IL-3Ra / CD123) is a humanized IgG1 monoclonal antibody against CD123...
Cat# A2431, SKU# A2431-1mg*25,
1mg*25, $5070.00
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Wei Li, et al.,
bioRxiv - Molecular Biology 2020
Quote:
Loss-of-function of HIS was performed by inhibiting the enhancer components with JQ1 (Selleck Chemicals Inc., S7110) as previously described (Suzuki et al. ...
-
No products found
because this supplier's products are not listed.
Jonathan M Downie, et al.,
bioRxiv - Genetics 2019
Quote:
... HEK293T cells were incubated in high glucose Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% heat inactivated fetal bovine serum (HI FBS, Genesee Scientific), and 1% pen/strep (P/S ...
-
No products found
Alex M. Jaeger, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... 100-200 mg of tumor bearing lung tissue was then thoroughly minced with Noyes scissors, prior to incubation with digestion buffer (HBSS supplemented with 5% HI FBS, 125 U/mL collagenase IV (Worthington) and DNase (Roche ...
-
No products found
because this supplier's products are not listed.
Raveen Rathnasinghe, et al.,
bioRxiv - Microbiology 2021
Quote:
... were maintained in Dulbecco’s Modified Eagle Medium (DMEM) complemented with 10% heat-inactivated Fetal Bovine Serum (HI-FBS; PEAK serum), penicillin-streptomycin (Gibco ...
-
No products found
because this supplier's products are not listed.
Aman Y. Husbands, et al.,
bioRxiv - Plant Biology 2022
Quote:
... Membranes were washed three times with PBS-T and incubated with 1:2000 anti-His-tag antibody (Abiocode M0335-1) for 1h with gentle shaking at RT ...
-
No products found
because this supplier's products are not listed.
MEENAKSHI TETORYA, et al.,
bioRxiv - Plant Biology 2023
Quote:
... The His-tagged peptides (GMA4CG_WT and GMA4CG_V6) phospholipid binding assays were performed using the PIP StripsTM (Echelon Biosciences Inc., CA) as previously described (Islam et al. ...
-
No products found
because this supplier's products are not listed.
Rui Fujii, et al.,
bioRxiv - Cell Biology 2024
Quote:
Sch91–183–6xHis was detected using mouse monoclonal anti-His antibody (AB10597733, cat. # D291–3; MBL International, Woburn, MA, USA). A C-terminal fragment of Sch9–5HA was detected using a mouse monoclonal anti-HA antibody (AB2565007 ...
-
No products found
because this supplier's products are not listed.
Nirupa Nagaratnam, et al.,
bioRxiv - Biochemistry 2022
Quote:
... The supernatant containing detergent solubilized His-tagged FopA was filtered through a 0.22 μm cellulose acetate membrane filter (Foxx Life Sciences) and loaded at 1 mL/min onto a Ni-NTA column (HisTrap HP ...
-
No products found
because this supplier's products are not listed.
Asha A. Philip, John T. Patton,
bioRxiv - Microbiology 2022
Quote:
... A puc19 plasmid containing a RIX/NSP3-2A-P-His insert under the control of a T7 transcription promoter (puc19/T7/ RIX/NSP3-2A-P-His) was purchased from Bio Basic Canada Inc ...
-
No products found
because this supplier's products are not listed.
Heng Lin, et al.,
bioRxiv - Neuroscience 2023
Quote:
... the immobilized FLAG-NBR1 was incubated with 1.5 μg His-MAPT and 1 μg GST or GST-BAG3 (Abnova H00009531-P01) in the BAG buffer (25 mM HEPES ...
-
No products found
because this supplier's products are not listed.
Yunlong Li, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... the full-length codon optimized gene with N-terminal 6x-His-sequnce and NdeI-XhoI sites was synthesized (IDT DNA Inc.) (Table S2) ...
-
No products found
because this supplier's products are not listed.
Oskar Staufer, et al.,
bioRxiv - Immunology 2022
Quote:
... beads were incubated with varying amount of the recombinant his-tagged proteins and corresponding MFIs were compared to reference beads of know AlexFluor molecule densities (Bangs Laboratories, IN).
-
No products found
because this supplier's products are not listed.
Boyang Zhao, et al.,
bioRxiv - Biochemistry 2023
Quote:
... and σNS-ΔN17 were subcloned into the bacterial expression vector pET28 with an N-terminal His tag and a TEV protease cleavage site (Epoch Life Science). Escherichia coli DE3 cells (Novagen ...
-
No products found
because this supplier's products are not listed.
Erica Misner, Min Zhang, Eva Sapi,
bioRxiv - Microbiology 2022
Quote:
... PCR products were analyzed by standard agarose gel electrophoresis in a 1% gel referenced against a Hi-Lo DNA ladder (Bionexus, Inc., Oakland, CA, USA; BN2050). An infected zebrafish sample which was positive for B ...
-
No products found
because this supplier's products are not listed.
Camilla Kjeldgaard Larsen, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... The cleaved proteins were incubated with Ni-NTA beads for revers his-purification and cleaved proteins were collected in the flow through and visualized on SDS-PAGE (Mini Protean Tris-Tricine Precast gels, BioRad).