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No products found
because this supplier's products are not listed.
Hari-Hara SK Potula, et al.,
bioRxiv - Microbiology 2019
Quote:
Borrelia burgdorferi sensu stricto low passage strain B31 was (obtained from the American Type Culture Collection Manassas, VA) used for MIC tests and all infection studies in C3H/HeN mice ...
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No products found
because this supplier's products are not listed.
Andrew C. Krusenstjerna, et al.,
bioRxiv - Microbiology 2022
Quote:
... burgdorferi B31 genomic DNA with Taq polymerase and TA cloned into pCR2.1 (Thermo Fisher). Constructs were sequenced to guarantee sequence fidelity.
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No products found
because this supplier's products are not listed.
Emily Petroni, et al.,
bioRxiv - Microbiology 2023
Quote:
... burgdorferi B31 (PA003) spirochetes were diluted into BSK-II or BSK-II +10mM spermidine (Sigma Aldrich, Catalog #S2626-1G) adjusted to pH 7.8 ...
-
No products found
because this supplier's products are not listed.
Justin A. Bosch, et al.,
bioRxiv - Genomics 2022
Quote:
... denatured proteins and Precision Plus Protein™ Dual Xtra Prestained Protein Standards (Biorad 1610377) were loaded into 16.5% Mini-PROTEAN® Tris-Tricine Gels (Biorad 4563066 ...
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No products found
because this supplier's products are not listed.
Michael J. Pereira, et al.,
bioRxiv - Microbiology 2021
Quote:
... burgdorferi B31-e2 expression strains using the DNeasy Blood and Tissue Kit (Qiagen). The open reading frame (lacking the putative lipoprotein signal sequence ...
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No products found
because this supplier's products are not listed.
Akihiro Fujikawa, et al.,
bioRxiv - Biochemistry 2019
Quote:
Protein extracts were precleaned with Protein G Sepharose (GE Healthcare) and subjected to immunoprecipitation with a combination of anti-FLAG M2 and Protein G Sepharose beads ...
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No products found
because this supplier's products are not listed.
Michael J. Pereira, et al.,
bioRxiv - Microbiology 2021
Quote:
... burgdorferi antibody (Abcam, ab20950) was added to two tubes ...
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No products found
because this supplier's products are not listed.
Bryan T. Murphy, et al.,
bioRxiv - Microbiology 2022
Quote:
... burgdorferi cells (62) using QuickLoad 2x Taq Master Mix (NEB, M0271) and primers 49 and 50 (Table 1) ...
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No products found
because this supplier's products are not listed.
Amy P Rapp, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Proteins were then digested with LysC at 1ug/100ug protein (Promega) for 3 h at room temperature (RT ...
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No products found
because this supplier's products are not listed.
Madhulika Singh, Aravind Penmatsa, Dipankar Nandi,
bioRxiv - Microbiology 2023
Quote:
Purified proteins and native protein standard (Supelco-Merck) were loaded in 4-15% gradient mini protean precast native gel (Bio-Rad ...
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No products found
because this supplier's products are not listed.
Adam E. Handel, et al.,
bioRxiv - Immunology 2021
Quote:
... and Protein block (Protein block (Dako) according to manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
L. Martin, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... F0425)/500 µg protein with protein A-agarose (Roche). Fished-out FGFR3 protein was eluted and then denatured at 95°C for 10 minutes in NuPAGE LDS sample buffer (Life Technologies ...
-
No products found
because this supplier's products are not listed.
Dharendra Thapa, et al.,
bioRxiv - Physiology 2019
Quote:
... Immunocaptured proteins were isolated using Protein-G agarose beads (Cell Signaling Technology), washed multiple times with CHAPS buffer ...
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No products found
because this supplier's products are not listed.
Ching-Yi Peng, Jia-Ping Wu,
bioRxiv - Molecular Biology 2020
Quote:
... 40μg of protein lysates will be incubated with anti-HDAC1 protein (from Santa Cruz, USA) followed by protein G-PLUS agarose precipitation ...
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No products found
because this supplier's products are not listed.
William K. Boyle, et al.,
bioRxiv - Microbiology 2020
Quote:
... burgdorferi dksA was commercially synthesized (GenScript, Piscataway, NJ, United States). The dksA gene was PCR amplified using primers listed in supplemental table 1 and cloned into the NheI site of the pMAL-C5X plasmid expression vector using a Gibson assembly kit (New England Biosciences ...
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No products found
because this supplier's products are not listed.
Xin Luo, et al.,
bioRxiv - Immunology 2024
Quote:
... of S protein) and recombinant S protein (Sino Biological) were used for stimulation at a final concentration of 2 μg/mL ...
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No products found
because this supplier's products are not listed.
Justin A. Peruzzi, et al.,
bioRxiv - Bioengineering 2023
Quote:
... purified FLAG tagged protein (recombinant p53 protein, R&D Systems) was run with vesicles to quantify loading ...
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No products found
because this supplier's products are not listed.
Kylene M. Harold, et al.,
bioRxiv - Physiology 2023
Quote:
... Total protein was stained (Revert Total Protein Stain, LI-COR) and imaged (Odyssey CLx ...
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No products found
because this supplier's products are not listed.
Yoshimi Kawamura, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Protein concentrations were measured using BCA Protein Assay Kit (TaKaRa). The samples were subjected to SDS-PAGE ...
-
No products found
because this supplier's products are not listed.
Rachel Baum, et al.,
bioRxiv - Cell Biology 2022
Quote:
A His-tagged mouse Capping Protein (F-actin-capping protein subunit alpha-1 and F-actin-capping protein subunit beta) plasmid was obtained from Addgene as described above (Plasmid #89950) ...
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No products found
because this supplier's products are not listed.
Tamara Weiss, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Protein extracts were stored in Protein LoBind tubes (Eppendorf) at −80°C ...
-
No products found
because this supplier's products are not listed.
Mohamed F. Hamed, et al.,
bioRxiv - Microbiology 2022
Quote:
... Housekeeping protein β-actin (a cytoskeleton protein; dilution, 1:5,000; BD Biosciences) was used as loading controls ...
-
No products found
because this supplier's products are not listed.
Sanjib Saha, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Protein expression was normalized to total protein (DM-TP01, Protein Simple) and compared between cell types.
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No products found
because this supplier's products are not listed.
Lalitha Yadhanapudi, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Proteins were labelled for 5 mins (for soluble proteins) or 15 mins (for membrane proteins) with 30 µCi of Express Protein Labelling Mix (Perkin Elmer) per sample at their restrictive temperature ...
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No products found
because this supplier's products are not listed.
Izumi Yanatori, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Equal amounts of extract protein were incubated with GFP-binding protein-conjugated agarose (Chromotek) or anti-RFP antibody (Anti-RFP RTU - Rockland Biosciences ...
-
No products found
because this supplier's products are not listed.
N Ogino, et al.,
bioRxiv - Cell Biology 2022
Quote:
... recombinant proteins Serpin E2 (BioLegend, 769002) and Serpin A3 (R&D systems ...
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No products found
because this supplier's products are not listed.
Stefan Kol, et al.,
bioRxiv - Bioengineering 2019
Quote:
... protein A biosensors (Pall) were equilibrated in sample diluent buffer (PBS containing 0.1% BSA and 0.02% Tween-20) ...
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No products found
because this supplier's products are not listed.
Qiuli Shi, et al.,
bioRxiv - Microbiology 2021
Quote:
Proteins were labeled using the Monolith NT Protein Labeling Kit RED (NanoTemper Technologies) according to the manufacturer’s protocol and using a buffer consisting of 20 mM HEPES ...
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No products found
because this supplier's products are not listed.
Alexandra C. Walls, et al.,
bioRxiv - Immunology 2020
Quote:
... Protein A biosensors (FortéBio/Sartorius) were first hydrated for 10 minutes in Kinetics buffer ...
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No products found
because this supplier's products are not listed.
David Gonzalez-Perez, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Proteins were separated by SDS-PAGE (12% Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) and transferred to PVDF membranes using an iBlot2 Gel Transfer Device (Thermo Fisher Scientific) ...
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No products found
because this supplier's products are not listed.
Panga Jaipal. Reddy, et al.,
bioRxiv - Biochemistry 2023
Quote:
Log phase B31 and MM1 Borrelia samples were analyzed using 5600+ Triple-TOF mass spectrometry (ABSciex, USA) coupled with Eksigent 400 nano-HPLC (Sciex, USA). Peptides from the 2 isolates were run separately by loading on trap column (200 μm × 0.5 mm ...
-
No products found
because this supplier's products are not listed.
Michał A. Surma, et al.,
bioRxiv - Biochemistry 2021
Quote:
... healthy diets differing by protein content: Teklad global 14% protein (Envigo #2014S, low protein) and Teklad global 18% protein (Envigo #2018S ...
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No products found
because this supplier's products are not listed.
Marion Thépaut, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Proteins were detected using InstantBlue protein stain (Expedeon) according to the supplier’s instructions.
-
No products found
because this supplier's products are not listed.
Xilin Wu, et al.,
bioRxiv - Microbiology 2021
Quote:
... the protein was coated to high protein-binding ELISA plates (Corning) at a concentration of 0.5 μg/ml ...
-
No products found
because this supplier's products are not listed.
May Daher, et al.,
bioRxiv - Immunology 2020
Quote:
... Cas9 protein (PNA bio) and gRNA were incubated at room temperature for 15 min in a 3:1 reaction with 15μg Cas9 and 5000 ng gRNA (2500ng of sgRNA#1 and 2500ng of sgRNA#2).47 The incubation product (gRNA and Cas9 complex ...
-
No products found
because this supplier's products are not listed.
Muaz Nik Rushdi, et al.,
bioRxiv - Immunology 2022
Quote:
... The purified proteins were biotinylated using biotin protein ligase (Avidity); excess biotin and ligase were removed with a Superdex 200 column (GE Healthcare) ...
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No products found
because this supplier's products are not listed.
Katarzyna Bogucka-Janczi, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Cdc42 and ARP3 proteins or ARP2/3 protein complex (Cytoskeleton) were used as bait and full-length GST-ERK3 (SignalChem ...
-
No products found
because this supplier's products are not listed.
Lorna Cryan, et al.,
bioRxiv - Biochemistry 2019
Quote:
... matrix protein (Rockland Immunochemicals ...
-
No products found
because this supplier's products are not listed.
Juliana Del Sarto, et al.,
bioRxiv - Microbiology 2021
Quote:
... non-structural protein 1 (NS1) and matrix protein 1 (M1) (Genetex) or cellular proteins such as tubulin ...
-
No products found
because this supplier's products are not listed.
Qiuling Wang, Suiwen Hou,
bioRxiv - Plant Biology 2022
Quote:
... Protein concentration was determined using BCA protein assay kit (Solarbio, PC0021).
-
No products found
because this supplier's products are not listed.
Eli Isael Maciel, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Protein concentrations were determined using a Bradford protein assay (VWR, E530-1L). In a 1.5mL tube ...
-
No products found
because this supplier's products are not listed.
Pei Xuan Lee, et al.,
bioRxiv - Microbiology 2020
Quote:
... or OVA protein (InvivoGen) was administered iv to the infected mice.
-
No products found
because this supplier's products are not listed.
Jürgen G. Okun, et al.,
bioRxiv - Physiology 2020
Quote:
... or protein-enriched (80%E protein, 10% carbohydrate, 10% fat; D16080303, Research Diets, USA) diets for a period of 3 weeks with various metabolic phenotyping tests ...
-
No products found
because this supplier's products are not listed.
Jiao Guo, et al.,
bioRxiv - Microbiology 2021
Quote:
... LASV GP1-Fc protein was incubated overnight with protein A+G Sepharose beads (ProteinTech) at 4°C and then washed five times with NETI buffer (50 mM Tris-HCl ...
-
No products found
because this supplier's products are not listed.
C Ferreras, et al.,
bioRxiv - Immunology 2020
Quote:
... and the complete sequence of the nucleocapsid phosphoprotein N and the membrane glycoprotein M (GenBank MN908947.3, Protein QHD43416.1, Protein QHD43423.2, Protein QHD43419.1) (Miltenyi Biotec, Germany). After 5 hours of stimulation ...
-
No products found
because this supplier's products are not listed.
Lei Zhang, José M Jiménez-Gómez,
bioRxiv - Plant Biology 2019
Quote:
... Total protein was extracted using PEB protein extraction buffer (Agrisera Antibodies) according to the manufacturer‘s instructions ...
-
No products found
because this supplier's products are not listed.
Stefanie Krug, et al.,
bioRxiv - Immunology 2021
Quote:
... Protein concentrations were determined by CB-X protein assay (G-Biosciences) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
How-Wing Leung, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Total protein was isolated using an RNA-protein extraction kit (Macherey-Nagel), as specified by the manufacturer ...
-
No products found
because this supplier's products are not listed.
Elaine C. Chen, et al.,
bioRxiv - Immunology 2021
Quote:
... Expressed protein was incubated with BioLock (IBA Lifesciences) and then isolated by Strep-tag affinity chromatography on StrepTrap HP columns (GE Healthcare) ...
-
No products found
because this supplier's products are not listed.
Yuan Gao, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 0.1ug recombinant human MUL1 Protein (H00079594-P01, Novus Biologicals). Bacterial expressed His-PABPC1/mutated PABPC1 was used as substrate.