-
No products found
because this supplier's products are not listed.
Alice Melocchi, et al.,
bioRxiv - Bioengineering 2023
Quote:
... with 5% AB human serum (Access Biologicals, UC-CA) and 1% L-glutamine (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Thibault Scalvenzi, et al.,
bioRxiv - Genomics 2021
Quote:
... 20 and 5 μm filters (nylon 40 μm cell strainer from BIOLOGIX; 20 μm net ring from Pharmacia Fine chemicals ...
-
No products found
because this supplier's products are not listed.
Patricia A Blundell, et al.,
bioRxiv - Immunology 2019
Quote:
Native influenza B Hong-Kong 5/72 was obtained from Meridian Life Sciences. To determine the optimal virus-to-erythrocyte ratio ...
-
No products found
because this supplier's products are not listed.
Alessandro Dema, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 5% CO2 in a humidified tissue culture incubator and regularly tested for mycoplasma contamination (IDEXX BioResearch). H1299 cells and sublines were previously authenticated by STR profiling [7] ...
-
No products found
because this supplier's products are not listed.
Olga Puchta, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
Microarray imaging was performed in an imaging buffer solution containing fluorophore DFHBI-1T ((Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1Himidazol-5(4 H)-one) (excitation = 472 nm, emission = 507 nm)) from Lucerna Technologies Cat ...
-
No products found
because this supplier's products are not listed.
Ling Zhou, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
Each confocal dish was first filled with 1 mL of 5 g/mL collagen (Helena Laboratories, TX, USA) and left at 4 °C overnight ...
-
No products found
because this supplier's products are not listed.
Vipul T. Vachharajani, et al.,
bioRxiv - Biophysics 2023
Quote:
... 3-5 mm wide slits were cut with a razor blade into a piece of Parafilm M (Bemis) and sandwiched between a glass slide (1 mm thick ...
-
No products found
because this supplier's products are not listed.
Sudeshna Saha, et al.,
bioRxiv - Evolutionary Biology 2021
Quote:
... 5% CO2 and shaking at 200 rpm in presence or absence of 30 µM CMP-Neu5Ac (Nacalai USA. Inc.) until OD600 equivalent to 0.4– 0.5.
-
No products found
because this supplier's products are not listed.
Mahshid Gazorpak, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 5% of the KH-103 in Captisol/DMSO was formulated in 20% Solutol (GLPBIO) in 0.9% sterile saline (Moltox) (w:v ...
-
No products found
because this supplier's products are not listed.
Elizabeth B. Draganova, et al.,
bioRxiv - Biophysics 2023
Quote:
... Reactions incubated for 5 min at 20 °C before imaging in 96-well chambered coverglass (Brooks Life Science Systems). Samples were imaged using a Nikon A1R Confocal Microscope with a 60x oil immersion lens at the Imaging and Cell Analysis Core Facility at Tufts University School of Medicine ...
-
No products found
because this supplier's products are not listed.
Kishor Dnyaneshwar Ingole, et al.,
bioRxiv - Plant Biology 2020
Quote:
... The membrane was blocked with 5% non-fat skim milk and western blots performed with indicated primary antibodies [anti-SNC1 (Abiocode), anti-PR1 or anti-PR2 (Agrisera) ...
-
No products found
because this supplier's products are not listed.
Bridget L Evans, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... 5 µm sagittal sections were taken from each tissue at a consistent depth and stained with Masson’s Trichrome (IHC World Masson’s Trichrome Staining Protocol for Collagen Fibres ...
-
No products found
because this supplier's products are not listed.
Mubasher Mohammed, et al.,
bioRxiv - Microbiology 2022
Quote:
... Mosquito midguts were collected in 1.5 ml Eppendorf tubes with 500μl of RPMI and homogenized using a micro-tube homogenizer (F65000-0000, SP-Bel-Art) at short intervals for 30 seconds ...
-
No products found
Yuxin Duan, et al.,
bioRxiv - Biophysics 2022
Quote:
... (Waltham, MA) N-hydroxyl succinimide-5 kDa PEG-biotin (NHS-PEG-biotin, HE041024-5K) was purchased from Biochempeg (Watertown, MA). 3-Aminopropyl triethoxysilane (APTES ...
-
No products found
because this supplier's products are not listed.
William J Dower, et al.,
bioRxiv - Immunology 2023
Quote:
... for 5-10 min at 25°C and the reaction was stopped using STOP solution (Surmodics Cat# LSTP-1000-01).
-
No products found
because this supplier's products are not listed.
Nobuyuki Oguri, et al.,
bioRxiv - Pathology 2023
Quote:
... 15 mg/kg n=5) was collected using 21-G needles into plastic syringes containing corn trypsin inhibitor (Molecular Innovations, Southfield ...
-
No products found
because this supplier's products are not listed.
Laurent Jacob, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Serial cross sections (5□µm thick) were immunostained with rabbit anti-mouse LYVE1 (1:100) polyclonal antibody (11-034, AngioBio Co). DAB (3,3′ -Diaminobenzidine ...
-
No products found
because this supplier's products are not listed.
W. Bai, et al.,
bioRxiv - Bioengineering 2020
Quote:
... or 5 mL of the gas phase was injected into a 15 mL helium-flushed septum-capped glass vial (Exetainer, Labco, Houston), using a HamiltonTM gas-tight syringe ...
-
No products found
because this supplier's products are not listed.
Minsuk Kong, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... Increasing ratios (multiplicities of incubation ranging from 20 to 150) of SSHELαHER2 (labeled with AlexaFluor647) were incubated with 5 × 105 cells/ml in siliconized tubes (G-tubes, Bio Plas Inc.) for 1 h at 37 °C with gentle inversion ...
-
No products found
because this supplier's products are not listed.
Laura Martinez-Ruiz, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Tumor pieces were digested using 5–10 mL of a 2.5 mg/mL Collagenase NB4 standard (S1745401 Nordmark Biochemicals, Uetersen, Germany) solution in PBS + 3 mM CaCl2 for 2 hours at 37°C ...
-
No products found
because this supplier's products are not listed.
Yana Roka-Moiia, et al.,
bioRxiv - Biochemistry 2023
Quote:
... 5 mM CaCl2) were incubated with 200 nM acetylated prothrombin and 100 pM factor Xa (Enzyme Research Laboratories, South Bend, IN) in 20 mM HEPES buffer (pH 7.4) ...
-
No products found
because this supplier's products are not listed.
Katherine J. Baxter, et al.,
bioRxiv - Microbiology 2023
Quote:
... the specimen was moved in the axial direction in 5 µm increments using a computer-controlled specimen stage (Optiscan III, Prior Scientific). Biofilms were incubated at 37°C with imaging performed at time 0 h and at 3 h intervals thereafter.
-
No products found
because this supplier's products are not listed.
Elizabeth C. Townsend, et al.,
bioRxiv - Microbiology 2023
Quote:
... the samples were stained with a PNA-FISH-TexasRed-5-conjugated universal bacterial (BacUni) 16s rRNA probe (AdvanDx, Woburn, MA, US), incubated and then counterstained with 3 µM 4′,6-diamidino-2-phenylindole (DAPI ...
-
No products found
because this supplier's products are not listed.
Chloe C. Ence, et al.,
bioRxiv - Microbiology 2024
Quote:
... The cultures were incubated at 37°C with 5% CO2 and routinely checked for mycoplasma contamination using the e-Myco Plus kit from Intron Biotechnology. Human hepatocellular carcinoma cells (HepG2 ...
-
No products found
because this supplier's products are not listed.
Sayak Mukhopadhyay, et al.,
bioRxiv - Microbiology 2024
Quote:
We washed the cell culture twice in motility buffer (MB) at 1500 g for 5 min in a centrifugation unit (Scilogex SCT412). The supernatant was discarded ...
-
Cat# F105,
3/pack, USD $939.00/Pack
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Suzanne M Johnson, et al.,
bioRxiv - Cell Biology 2020
Quote:
... was centrifuged in 2 tubes to remove cells (300 × g 5 minutes, × 2) and filtered using a double layered 5µm pore nylon Sieve (Fisher Scientific: BioDesign cat 12994257). The supernatant was collected and centrifuged at 2000 × g for 30 minutes and prepared for ISX analysis ...
-
No products found
because this supplier's products are not listed.
Hongbing She, et al.,
bioRxiv - Genomics 2020
Quote:
... The PCR was performed in a total reaction volume of 10 μL containing 5 μL 2×Taq Master Mix (CoWin Biosciences, China), 0.25 μL forward and reverse primer ...
-
No products found
because this supplier's products are not listed.
Cassandra M. Stawicki, et al.,
bioRxiv - Bioengineering 2021
Quote:
... Aliquots of 2-5 µl of sample were added to 700 µl of 2% PBS and loaded into a cuvette (BrandTech Scientific, 759150). Zeta potential was measured in Folded Capillary Cell cuvettes (Malvern ...
-
No products found
because this supplier's products are not listed.
Glory Nasseri, et al.,
bioRxiv - Neuroscience 2021
Quote:
... resuspended proteins were PEGylated for 1 hr at RT to label newly exposed cysteine thiols with the 5 kDa mass tag reagent (mPEG-5k, Badrilla SiteCounter™). Approximately 20 μl of each supernatant was saved as the “total input.” For immunoblot analysis ...
-
No products found
because this supplier's products are not listed.
Sang-Hee Lee, Seunghyung Lee,
bioRxiv - Cell Biology 2019
Quote:
... The membranes were then washed three times with TBS-T each 5 min and incubated with secondary antibodies conjugated horseradish peroxidase and visualized using the West Save Enhanced Chemiluminescence kit (AbFrontier, Austin, TX). Protein expression was measured using the EZ-Capture II system (ATTO ...
-
No products found
because this supplier's products are not listed.
Whasil Lee, et al.,
bioRxiv - Molecular Biology 2020
Quote:
Paraffin-embedded tissue sections (15 µm) of human articular cartilage from healthy (n=5) and OA-positive (n=6) anonymized donors were used (Articular Engineering, IL). Sections were immunolabeled with Piezo1-antibody (NBP1-78537 ...
-
No products found
because this supplier's products are not listed.
Ana Belen Lopez-Rodriguez, et al.,
bioRxiv - Neuroscience 2022
Quote:
... mice (n=6) underwent stereotaxic surgery to implant MBR-5 intracerebral guide cannulae (ID 457μm, OD 635 μm; BASi Research Products, USA) into the striatum ...
-
No products found
because this supplier's products are not listed.
Devin Rocks, et al.,
bioRxiv - Neuroscience 2023
Quote:
... n=5/sex/group) were rinsed with distilled water and underwent the Golgi-Cox staining procedure (Golgi-Cox OptimStain Kit, Hitobiotec Inc. #HTKNS1125) following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Albéric A. de Lajarte, et al.,
bioRxiv - Biochemistry 2024
Quote:
... adding a T7 promoter sequence at the forward primer (5′ TAATACGACTCACTATAG 3′) using a 2X PCR PreMix (Syd Labs, Cat. MB067-EQ2N), human cDNA (ZYAGEN ...
-
No products found
because this supplier's products are not listed.
Le Xu, et al.,
bioRxiv - Cell Biology 2024
Quote:
... Primary antibodies with final concentrations used for immunofluorescence staining: rabbit anti-SCGB1A1 polyclonal antibody [5 mg/ml] (WRAB-3950, Seven Hills Bioreagents), mouse anti-FOXJ1 monoclonal antibody [8 mg/ml] (14-9965-80 ...
-
No products found
because this supplier's products are not listed.
James M. Fulcher, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 50-cm length) were slurry-packed with C2 packing material (5 µm and 3 µm for trap/analytical respectively, 300 Å, Separation Methods Technology). Samples were loaded into a 5 µL loop ...
-
No products found
because this supplier's products are not listed.
Valerija Dobricic, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Hybridization buffers containing 5’ HEX-labeled probes were used to incubate slices in an in-situ hybridization apparatus (Boekel Slide Moat, Feasterville, Pennsylvania) at 52 °C under the condition of heat preservation and humidification ...
-
No products found
because this supplier's products are not listed.
Angus M Sidore, et al.,
bioRxiv - Synthetic Biology 2019
Quote:
... The individual wells were then washed >5 times using 2X Bind & Wash Buffer and a 384-Well Post Magnetic Plate (Permagen Labware, Peabody, MA). After washing ...
-
No products found
because this supplier's products are not listed.
Yongle Chen, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... were pretreated by overnight incubation at 37°C with a 10 µL mixture of 5 ng/µL dodecyl-beta-D-maltoside (DDM, J&K Scientific, China) and 1 ng/µL trypsin (Promega ...
-
No products found
because this supplier's products are not listed.
Rebecca C.S. Edgar, et al.,
bioRxiv - Microbiology 2023
Quote:
... Venous blood (5 mL) was collected by venipuncture and after removal of host white blood cells using Plasmodipur filters (EuroProxima B.V., The Netherlands), packed infected red blood cells (iRBCs ...
-
No products found
because this supplier's products are not listed.
Tianyi Zhang, et al.,
bioRxiv - Immunology 2023
Quote:
... day 0 and day 21) with 5 μg/dose of recombinant SARS-CoV-2 Spike protein (S1+S2 extracellular domain) (Bon Opus Biosciences, #BP040) adjuvanted with 100 μg/dose alhydrogel adjuvant 2% (InvivoGen ...
-
No products found
because this supplier's products are not listed.
Max F. Moescheid, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 10 µM of 5’amine C6 primers (IDT, Coralville, IA) were incubated with 1 mM Bis-PEG10-NHS ester (BroadPharm, San Diego, CA) in borate buffer (Thermo Fisher ...
-
No products found
because this supplier's products are not listed.
Sara Meril, et al.,
bioRxiv - Cancer Biology 2023
Quote:
0.5 μg RNA was mixed with 4 μL 5X reaction buffer and 1 μL RTase (AzuraQuant cDNA synthesis kit, Azura Genomics cat: AZ1996). Reaction mix was incubated at 42°C for 30 min and denatured at 85°C for 10 min ...
-
Cat# AB-T080,
50 microliters,USD $330.0
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Joël S. Bloch, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... the biotinylated samples were desalted into PBS and were mixed at a concentration of 5 μM with a 2.4-fold molar excess of Streptavidin-ZAP (Advanced Targeting Systems, Carlsbad, CA, USA). The mixture was incubated for 1h on ice before diluting it in PBS to a working concentration of 500 nM.
-
No products found
because this supplier's products are not listed.
Waja Wegner, et al.,
bioRxiv - Neuroscience 2021
Quote:
... The white light was spectrally filtered for green light with a bandpass filter (BP1, BrightLine HC 520/5, Semrock, IDEX Health & Science, Rochester, NY) for selective excitation of EYFP or Citrine at 520 nm (Exc2 ...
-
No products found
because this supplier's products are not listed.
Roman Mylonas, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Entire lanes were cut into 45-47 identical gel slices using a gridcutter tool (MEE1-5-50, The Gel Company, San Francisco, CA, USA) (Supplementary Fig ...
-
No products found
because this supplier's products are not listed.
Cameron V. Fili, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... mice were placed in individually ventilated caging at a housing density of 5 mice per cage on corncob bedding (Shepherd Specialty Papers, Watertown, TN) with a 12:12 light ...
-
No products found
because this supplier's products are not listed.
Jian Zhang, et al.,
bioRxiv - Immunology 2022
Quote:
... were cocultured with autologous memory B cells (5×104 cells) in the presence of 100 ng/ml staphylococcal enterotoxin B (SEB) (Toxin Technology, Sarasota, FL, USA) and RPMI 1640 medium supplemented with 10% FBS in 96-well U-bottom plates for 6 days ...
-
No products found
because this supplier's products are not listed.
Oluwaseun M. Ajayi, et al.,
bioRxiv - Physiology 2022
Quote:
... Mosquito eggs were produced from 4-5 weeks old females through artificial feeding (Hemotek, Blackburn, United Kingdom) with chicken or rabbit blood (Pel-Freez Biologicals, Rogers, AZ, USA). Upon egg hatching ...
-
No products found
because this supplier's products are not listed.
Lisanne de Vor, et al.,
bioRxiv - Microbiology 2021
Quote:
... antibodies were buffer exchanged into conjugation buffer and incubated at 37°C for 1.5 h with a 5-fold molar excess of bifunctional CHXA” (Macrocyclics, prepared less than 24 h before use). The mAb-CHXA” conjugate was then exchanged into 0.15 M ammonium acetate buffer to remove unbound CHXA” and subsequently incubated with approximately 150 kBq 111In (purchased as [111In]InCl3 from CuriumPharma ...