Adithya Ramesh, et al.,bioRxiv - Synthetic Biology 2022
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... and 4 were cloned into the AarI digested pCas9yl-GW vector using the Gibson Assembly HiFi HC 1-step Master Mix (SGI-DNA). Library 2 was digested with AarI and cloned into pCas9yl-GW digested with AarI using Golden Gate assembly with T4 DNA ligase (NEB).
Kevin M. Tharp, et al.,bioRxiv - Cell Biology 2020
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... 1 μL of sample was separated over a 25 cm column packed with 1.9 μm Reprosil C18 particles (Dr. Maisch HPLC GmbH) by a nanoElute HPLC (Bruker) ...
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Human immature dendritic cells (imDCs) were obtained from peripheral blood mononuclear cells (PBMC) from HIV-1 seronegative donors using a Ficoll-Hypaque gradient (Alere Technologies AS). The monocyte population was selected by adherence on a T75cm2 flask for 1 hour ...