Laura Ohl, et al.,bioRxiv - Molecular Biology 2023
Quote:
... Tissue was mixed with 1 mL 40:40:20 methanol:acetonitrile:water (extraction solvent) and homogenized with a benchtop lyser (SciLogex OS20-S) at 1600 mhZ for approximately 15 sec ...
Adithya Ramesh, et al.,bioRxiv - Synthetic Biology 2022
Quote:
... and 4 were cloned into the AarI digested pCas9yl-GW vector using the Gibson Assembly HiFi HC 1-step Master Mix (SGI-DNA). Library 2 was digested with AarI and cloned into pCas9yl-GW digested with AarI using Golden Gate assembly with T4 DNA ligase (NEB).
Kathleen L. Arnolds, et al.,bioRxiv - Microbiology 2023
Quote:
Mesocosm experiments were conducted in 3D-printed pots (S. Fig. 1) that have 18 sampling ports which are sealed with neoprene stoppers (Eisco Labs, Victor, NY). A perforated base allows for root development ...
Quote:
... Those samples were submitted for Lyme disease serology and subjected to two-tiered testing consisting of (Tier 1) a C6 peptide screen (Immunetics®; C6 Lyme ELISA™) or Enzyme Linked Fluorescent Assay (ELFA ...