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WB, IHC,ELISA
Cat# A5444, SKU# A5444-20ul,
20ul, $47.00
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Kristina A.M. Arendt, et al.,
bioRxiv - Cancer Biology 2020
Quote:
In vitro cell proliferation was determined using WST-8 [water soluble tetrazolium-8 or 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulphophenyl)-2H-teterazolium] assay (Bimake; Munich, Germany). For this ...
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No products found
because this supplier's products are not listed.
Andrew Barszczyk, et al.,
bioRxiv - Cell Biology 2023
Quote:
... alkaline phosphatase conjugated antibodies were detected colourometrically using BCIP/NBT (nitro-blue tetrazolium and 5- bromo-4-chloro-3’-indolyphosphate) Substrate Solution (#BE116.100ML; Bio Basic Canada Inc). For analysis of bands ...
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No products found
because this supplier's products are not listed.
Thomas W. Jackson, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... and 2H,2H,3H,3H-Perfluorooctane-1-sulfonate (6:2 FTSA, CAS 59587-39-2, purity ≥ 97%) were from Synquest Laboratories (Alachua, FL). HSA (CAS 70024-90-7 ...
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No products found
because this supplier's products are not listed.
Amada M. Abrego, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Rats were induced with 3-4% isoflurane (SomnoSuite, Kent Scientific) and all whiskers on the right facial pad were trimmed except B1 ...
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No products found
because this supplier's products are not listed.
Teng-Chieh Yang,
bioRxiv - Bioengineering 2023
Quote:
... One (1) μm yellow PSP and 6 μm red PSP were from Polyscience Inc ...
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No products found
because this supplier's products are not listed.
Stephen J. DeCamp, et al.,
bioRxiv - Biophysics 2020
Quote:
Polymerized polyacrylamide gels were first treated with 440 μL of a 1:50 (v/v) mixture of Sulfosuccinimidyl 6-(4'-azido-2'-nitrophenylamino)hexanoate (Sulfo-SANPAH) (ProteoChem) and 50 mM HEPES solution under a UV light for 10 minutes ...
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No products found
because this supplier's products are not listed.
Katelyn A. Bustin, et al.,
bioRxiv - Neuroscience 2023
Quote:
... (Bullet Blender, BBY24M model, Next Advance, Inc., speed 8, 3 min, 4 °C), samples were diluted in PBS (500 μL ...
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No products found
because this supplier's products are not listed.
M. Martinez, et al.,
bioRxiv - Microbiology 2023
Quote:
... at 4°C and loaded 3 times in a CellD disrupter (Constant Systems). The lysate was centrifuged for 15min at 12000 x g at 4°C to remove cell debris and the supernatant was centrifuged again for 1h at 100000 x g at 4°C ...
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No products found
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Jérémy Scutenaire, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... membranes were blocked in 5% milk PBS-T (1X PBS buffer with 0.1% Tween-20) and incubated overnight at 4°C with one of the following antibodies: anti-HA.11 (1:2,000; Eurogentec, Cat# MMS-101P-500), anti-Stm1 (1:10,000 ...
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No products found
because this supplier's products are not listed.
Kevin R. Trabbic, et al.,
bioRxiv - Immunology 2021
Quote:
β-1,3(1-6) glucan (Yeast Beta Glucan, Megazyme, Bray, Ireland; 3 mg) was suspended in 4.9 mL MilliQ H2O contaning 100 μL of 1 M NaOH ...
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No products found
because this supplier's products are not listed.
Martin Meschkat, et al.,
bioRxiv - Neuroscience 2020
Quote:
... one hemisphere was homogenized in 4 ml modified RIPA buffer using an T-10 basic Ultra-Turrax (IKA, IKA®-Werke GmbH & CO ...
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No products found
because this supplier's products are not listed.
Martineau Éric, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Muscles were incubated overnight at 4°C with a rat IgG2a anti-MAC-2 (Galectin-3) antibody (1:250; clone M3/38; Cedarlane, cat# CL8942AP), then 2h at RT with a rabbit IgG anti-S100β antibody (1:250 ...
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No products found
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Susanna Sauret-Güeto, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... and 5 Channel Spectral Scanhead (4 hybrid detectors and 1 PMT). For slides ...
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No products found
because this supplier's products are not listed.
Hiroshi Nishiyama, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Approximately 200-300 nl of virus was injected over 4-6 minutes using a Nanoject II injector (Drummond Scientific). This injection coordinates primarily label the interposed nucleus of the deep cerebellar nuclei ...
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No products found
because this supplier's products are not listed.
Carena Cornelssen, et al.,
bioRxiv - Bioengineering 2023
Quote:
... using 4% paraformaldehyde (Thomas Scientific) in 1x phosphate buffer saline (Dulbecco’s) ...
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No products found
because this supplier's products are not listed.
Hailey I. Edelstein, et al.,
bioRxiv - Synthetic Biology 2024
Quote:
... UltraRainbow Calibration Particles (Spherotech #URCP-100-2H) were run with each flow cytometry experiment ...
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No products found
because this supplier's products are not listed.
Xueqin Li, et al.,
bioRxiv - Cell Biology 2019
Quote:
... DNA (3 μg) was sonicated at intensity 4 for 200 cycles per burst for 55 s (Covaris S2), and DNA fragments were end-repaired ...
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No products found
because this supplier's products are not listed.
Lauren Kane, et al.,
bioRxiv - Genetics 2021
Quote:
... and Chroma #89014ET (3 colour) or #89000ET (4 colour) single excitation and emission filters (Chroma Technology Corp., Rockingham, VT) with the excitation and emission filters installed in Prior motorised filter wheels ...
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No products found
because this supplier's products are not listed.
Sutanuka Chakraborty, et al.,
bioRxiv - Microbiology 2020
Quote:
All the flow cytometry data analyses were performed using FCS Express 4 and 6 versions (De Novo Software, Los Angeles, CA).
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No products found
because this supplier's products are not listed.
Alexis S Mobley, et al.,
bioRxiv - Immunology 2021
Quote:
... and APC-IL-4 (Tonbo Bioscience) for 30 minutes at 4◦C ...
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No products found
because this supplier's products are not listed.
Ariane Zutz, et al.,
bioRxiv - Biochemistry 2020
Quote:
... The isolated fractions were separated on SDS-PAGE (RunBlue 4-20 %, Expedeon; NuPAGE®Bis-Tris gel 4-12%, Invitrogen) and analysed by InstantBlue staining (Expedeon ...
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No products found
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Yuta Kudo, et al.,
bioRxiv - Biochemistry 2020
Quote:
... in 250 ml Erlenmeyer flasks containing a stainless steel spring and grown for 3 or 4 days at 28°C with shaking at 230 RPM (New Brunswick Innova 2300).
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No products found
because this supplier's products are not listed.
Ding Xiong, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 2 to 3×106 cells were seeded in one 35mm glass bottom culture dishes (MatTek) after transient transfection ...
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No products found
because this supplier's products are not listed.
W. Grant Ludlam, et al.,
bioRxiv - Biochemistry 2019
Quote:
The BBS2-7-9 complex was purified at 4°C by affinity purification using 4 mL of Strep-Tactin resin (IBA Life Sciences) packed in a 2 cm diameter column and equilibrated with equilibration buffer (20 mM HEPES pH 7.5 and 20 mM NaCl) ...
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No products found
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Alex Matsuda, et al.,
bioRxiv - Microbiology 2022
Quote:
... 4 µl of 16× SAH-d2 conjugate solution (Cisbio) was added ...
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No products found
because this supplier's products are not listed.
Leland B. Hyman, et al.,
bioRxiv - Bioengineering 2020
Quote:
We subcultured MOLT-4 cells (American Type Culture Collection) in a 1:8 ratio every two days in RPMI-1640 Medium (Gibco ...
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No products found
because this supplier's products are not listed.
Qi Feng, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... and incubated overnight at 4°C with primary antibodies against DCBLD1 (1:30, Atlas antibody, Bromma, Sweden) and CD31 (1:100 ...
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Cat# 13735-13-2,
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Alexandra Gros, et al.,
bioRxiv - Neuroscience 2023
Quote:
... These rats also received one intraperitoneal injection of 5-Bromo-2’-deoxyuridine (BrdU, 200 mg/kg in 0.9% NaCl, BOC Sciences 59-14-3) 24h before sacrifice to study adult newborn cell proliferation after several days of simulated microgravity exposure and to reduce the number of animals used for this study.
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No products found
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Shivneet K. Gill, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Dilutions of human serum (Human Serum age 4-6, Innovative Research) were performed in TBSM ...
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No products found
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Tanja Bhuiyan, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... cells were incubated in 2 μg/mL 4′,6-diamidino-2-phenylindole (DAPI, GeneCopoeia, C002) in PBS for 5 min at RT ...
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No products found
because this supplier's products are not listed.
Yuan Wang, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... 4-1BB (Acrobiosystems) or BSA (Solarbio ...
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No products found
because this supplier's products are not listed.
Farhan Ali, et al.,
bioRxiv - Neuroscience 2019
Quote:
... A bundle of stainless steel wires (3-4 wires per bundle) (790500, A-M Systems) was inserted into RSC ...
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No products found
because this supplier's products are not listed.
Mikael G. Pezet, et al.,
bioRxiv - Cell Biology 2023
Quote:
... hPSCs were passaged every 3-4 days with Accutase (Innovative Cell Technologies, San Diego, CA) at least two times before differentiation ...
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No products found
because this supplier's products are not listed.
Miriam Pagin, et al.,
bioRxiv - Genetics 2021
Quote:
... wild-type and Sox2-deleted neurospheres were grown for 2-3 passages (3-4 days each) in FM supplemented with bFGF (10ng/ml, Tebu-bio) and EGF (10 ng/ml ...
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No products found
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Coraline Mercier, et al.,
bioRxiv - Microbiology 2022
Quote:
... DNA extraction, library preparation, sequencing (Pacific Biosciences Sequel, 4 samples on one SMRT cell) and assembly (‘Microbial Assembly’ function in PacBio SMRT® Link v8.0 for ACAM34_DSMZ and flye assembly tool v2.6 [65] for ACAM34_UNSW ...
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No products found
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Zhaoquan Wang, et al.,
bioRxiv - Immunology 2023
Quote:
... and harvested brains were submerged in 4% formaldehyde (StatLab, 28530-1) for 24-48 hours at 4°C ...
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No products found
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Christina Strauch, et al.,
bioRxiv - Neuroscience 2024
Quote:
... and nuclei were visualized using 4’,6-diamidino-2-phenylindole (DAPI) in mounting medium (SCR-038448; Dianova, Hamburg, Germany).
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No products found
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Alejandro Villalba, et al.,
bioRxiv - Immunology 2023
Quote:
... were stimulated as described [6] in flat-bottom 96 well plates with a soluble anti-CD3 IgE subclass (T3/4.E, Sanquin, Amsterdam ...
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RatCol® for 2D coatings is 20 mL of 4 mg/ml, type I rat tail telocollagen solution for 2D...
Cat# 5056-20ML,
20 mL, USD $175.0
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Rezvan Parvizi, et al.,
bioRxiv - Genomics 2024
Quote:
... which is a mix of one part collagen (Advanced BioMatrix, 6 mg/mL), one part HEPES (pH 8) ...
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No products found
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Sangderk Lee, et al.,
bioRxiv - Neuroscience 2023
Quote:
... plated into a single well of a 6 well dish that was precoated with Matrigel solution (.4% dissolved in complete astrocyte medium; ScienCell #254277) and grown for two days at 37°C ...
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No products found
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Siyuan Zhao, et al.,
bioRxiv - Neuroscience 2021
Quote:
... (4) Spin-coating LOR3A photoresist (MicroChem) at 4000 rpm ...
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No products found
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Anqi Zhang, et al.,
bioRxiv - Bioengineering 2023
Quote:
4-0 silk sutures (Fine Science Tools) were used to ligate the ECA and temporarily stop blood flow in CCA and posterior auricular artery (PAA) ...
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No products found
because this supplier's products are not listed.
Desirée Böck, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Brain sections were incubated with primary antibodies overnight at 4°C (rabbit-NeuN, 1:1’000, abcam 177487; mouse-TH; 1:1’000, Immunostar 22941 ...
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No products found
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Bibiana Rius, et al.,
bioRxiv - Biochemistry 2020
Quote:
... 1.6 mM BTTAA ligand (2-(4-((bis((1-tert-butyl-1H-1,2,3-triazol-4-yl)methyl)amino)methyl)−1 H-1,2,3-triazol-1-yl)acetic acid) (Click Chemistry Tools Scottsdale, Az), and 5 mM sodium ascorbate ...
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No products found
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Angela M. Phillips, et al.,
bioRxiv - Evolutionary Biology 2022
Quote:
... and 4% polyethylene glycol 200 (Hampton Research, #HR2-084), respectively ...
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No products found
because this supplier's products are not listed.
Diego Giraldo, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 20μL per paper) and the papers were inserted into 3cm segments of 1/4″ vinyl tubing (06405-02, Cole-Parmer). The cartridge was capped on both ends with parafilm until ready to use ...
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No products found
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Diego J. Páez-Moscoso, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... and separated on a 4% (v/v) polyacrylamide (29:1) gel containing 8 M urea and transferred to Biodyne nylon membrane (Pall Corporation) at 400 mA for 1 hour in 0.5× TBE buffer ...
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No products found
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Natsumi Takei, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... eluted with elution buffer (4% BrdU/DMSO solution in nuclease-free PBS; MBL International), and subjected to SDS-PAGE ...
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No products found
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Lucia Grenga, et al.,
bioRxiv - Microbiology 2020
Quote:
... In each replicate the samples were labelled with an iTRAQ 4 plex kit (Sciex) as follows ...
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No products found
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Emily G. Kuiper, et al.,
bioRxiv - Biochemistry 2019
Quote:
... was monitored during unfolding over a linear temperature gradient (35-95 °C) over 3 minutes on a Tycho NT.6 instrument (NanoTemper). The temperature at which a transition occurs in a plot of the first derivative of the fluorescence ratio (350/330 nm) ...