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Building Block
Sold for research purposes only.
Cat# 1284.0, SKU# 1284-1000 mg,
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Isabel R.K. Kuebler, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... The MCH receptor antagonist 6-(4-Chloro-phenyl)-3-[3-methoxy-4-(2-pyrrolidin-1-yl-ethoxy)-phenyl]-3H-thieno[3,2-d]pyrimidin-4-one (GW803430) (Axon Medchem, Groningen, Netherlands) was freshly prepared the day of the treatment ...
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No products found
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Morgan E. Mouchka, et al.,
bioRxiv - Evolutionary Biology 2019
Quote:
... Colonies were screened on LB plates with 50 μg/mL ampicillin and 9.5 mg/mL X-gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside; APExBIO) and Sanger sequencing of the J was used to confirm cloning and successful transformation of the ancestral λ sequence.
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No products found
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Pazhanichamy Kalailingam, et al.,
bioRxiv - Pathology 2024
Quote:
... then incubated overnight at 37°C with staining buffer (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside [X-gal, Bioline, London ...
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No products found
because this supplier's products are not listed.
Subash Godar, et al.,
bioRxiv - Biophysics 2021
Quote:
... We detected the bound alkaline phosphatase labeled antibodies by incubating the membrane in BCIP/NBT (5-bromo, 4-chloro, 3- indolylphosphate/nitro-blue tetrazolium, AMRESCO, LLC.) substrate for 30–45 minutes ...
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No products found
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Taylor Hart, et al.,
bioRxiv - Neuroscience 2022
Quote:
... 95% 4-methyl-3-hexanol was purchased from Enamine (CAS# 615-29-2), and paraffin oil from Hampton Research (cat ...
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No products found
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Stephen J. DeCamp, et al.,
bioRxiv - Biophysics 2020
Quote:
Polymerized polyacrylamide gels were first treated with 440 μL of a 1:50 (v/v) mixture of Sulfosuccinimidyl 6-(4'-azido-2'-nitrophenylamino)hexanoate (Sulfo-SANPAH) (ProteoChem) and 50 mM HEPES solution under a UV light for 10 minutes ...
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No products found
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Kazuhiro Hayashi, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 25mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)(Research Products International) or acidic pH 6.5 media (26mM NaHCO3 ...
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No products found
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Trinovita Andraini, et al.,
bioRxiv - Neuroscience 2021
Quote:
... 3-(2,4-Dichloro-5-methoxyphenyl)-2-sulfanyl-4(3H)-quinazolinone (BML-CM127-0050, Enzo Life Sciences) was injected at 20mg/kg (in 10% DMSO ...
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No products found
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Jung Ho Yu, et al.,
bioRxiv - Bioengineering 2022
Quote:
... and glutathione diethyl ester (GSHDE, BACHEM)-coated gold nanoclusters (GSHE-Au and GSHDE-Au nanoclusters) ...
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4-bromo-3-hydroxybenzoic acid is a potent inhibitor of the enzyme histidine decarboxylase with...
Cat# S5444, SKU# S5444-25mg,
25mg, $97.00
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Liying Chen, et al.,
bioRxiv - Neuroscience 2023
Quote:
The D1 receptor antagonist R(+)-7-chloro-8-hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepi ne hydrochloride (SCH-23390, Selleck chemicals, China) was dissolved in 0.9% saline and stored -80°C ...
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No products found
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Ana S Almeida, et al.,
bioRxiv - Microbiology 2021
Quote:
... and three 3–4 mm sterile glass beads (Biospec Products). Next ...
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No products found
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Urszula Kozłowska, et al.,
bioRxiv - Cell Biology 2021
Quote:
... (TLR4, Bioss, 1:200, overnight, +4□C), (CD11b ...
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No products found
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Wenjing Zhang, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The membranes were then incubated with the following primary antibodies at 4°C for 6 h: GAPDH (1:6000, no. A19056), vinculin (1:1000, A2752) (ABclonal Technology, Co., China), caspase-3 (1:1000 ...
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No products found
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Yuchen Li, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Organoid sections (4 µm) were probed with anti-cleaved caspase 3 (Biocare Medical) in 10% goat serum for 2 hours at room temperature and subsequently stained with Mach 1 Universal Polymer HRP ...
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No products found
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Hao Guo, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Germany) with a guard column (EC 4/3 NUCLEODUR C18 Gravity, 3 μm, Macherey-Nagel GmbH & Co. KG Germany), while the column temperature was kept at 40 °C in a thermostatic column compartment (Thermo Fisher UltiMate 3000) ...
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35 mm glass bottom dish with 4 chambers, 20mm microwell, #1 cover glass (0.13-0.16mm). Designed...
Cat# D35C4-20-1-N,
100/case, $184.00
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Taemoon Chung, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... 3×105 MIA PaCa-2/GemLuc cells were either plated day -1 on a black-walled 6 well plate (Cellvis, Mountain View, CA) or a clear bottom 6 well plate (Corning ...
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Wander Van Breedam, et al.,
bioRxiv - Bioengineering 2021
Quote:
... from Bio-Rad (4–20% Mini-PROTEAN® TGX™ Precast Protein Gels ...
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No products found
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Leticia Peris, et al.,
bioRxiv - Neuroscience 2021
Quote:
... at 4°C overnight and 1 h with appropriate secondary antibodies (LI-COR Biosciences). Image acquisition was performed with an Odyssey infrared imaging system (LI-COR Biosciences ...
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No products found
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Michele Bortolomeazzi, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... the slides were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Akoya Biosciences) and coverslipped using ProLong Gold antifade mounting media (Thermo Fisher Scientific) ...
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No products found
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Georgina Gyarmati, et al.,
bioRxiv - Physiology 2021
Quote:
... animals underwent brief anesthesia sessions every 3–4 days using 1%–2% isoflurane and the SomnoSuite low-flow anesthesia system (Kent Scientific, Torrington, CT). Body temperature was maintained with a homeothermic blanket system (Harvard Apparatus) ...
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No products found
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Lara Hassan, et al.,
bioRxiv - Genetics 2019
Quote:
... 80 μg 4-Nitrophenyl-β-D-mannopyranoside (Megazyme, Ireland, O-PNPBM) and 0.0025 μg of purified enzyme ...
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No products found
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Michèle Reindl, et al.,
bioRxiv - Microbiology 2020
Quote:
... Quantitative Gus and LacZ liquid assays were based on the chromogenic and fluorescent substrates ONPG for the liquid LacZ activity assay (o-nitrophenyl-β-D-galactopyranoside) and MUG for the liquid Gus activity assay (4-methylumbelliferyl-ß-D-glucuronide trihydrate; BioWorld, 30350000-2 (714331), respectively (Stock et al. ...
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No products found
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Bradley C. Paasch, et al.,
bioRxiv - Plant Biology 2023
Quote:
... blocked in 3% milk + 2% BSA and immunoblotted overnight at 4 °C with antibodies specific to Arabidopsis FLS2 (Agrisera), BAK1 (Agrisera) ...
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No products found
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Glenn F. W. Walpole, et al.,
bioRxiv - Cell Biology 2021
Quote:
... PtdIns(4)P diC16 (Echelon Biosciences P-4016-2); PtdIns(3,4)P2 (Echelon Biosciences P-3416-2) ...
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No products found
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Justin M. Westerfield, et al.,
bioRxiv - Biophysics 2021
Quote:
... the peptides were labeled at their N-terminus with an amine-reactive environmentally sensitive fluorescent reporter, Succinimidyl 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoate (NBD-X, SE) (AnaSpec, Fremont, CA) [37] ...
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No products found
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Seung Jae Shin, et al.,
bioRxiv - Bioengineering 2024
Quote:
... Membrane (RayBiotech, #AAM-BLM-1-4). The analysis and data acquisition were conducted by ebiogen Inc ...
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No products found
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Brian T. Do, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... with a 4:2:1:1 ratio of the target:pMDLg:pMD2.G:pRSV-REV plasmids using Transit 293T reagent (Mirus). After 48 hours ...
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No products found
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Li-Qiang Chen, et al.,
bioRxiv - Neuroscience 2022
Quote:
... and a series of calibrated von Frey filaments (2, 4, 6, 8, 10, 15, 26 g, Stoelting Company, Wood Dale, Illinois, USA) were lightly applied to the skin within the infraorbital territory ...
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Iliodora V. Pop, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Mice (1-2 months old) were injected with 4% (w/v) FG solution in saline (Fluorochrome). Mice were anesthetized with isoflurane and the area above and around the cerebellar region was prepared for surgery ...
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No products found
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Helmut Bischof, et al.,
bioRxiv - Cancer Biology 2023
Quote:
Glucose uptake was assessed using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2- NBDG) (Biomol GmbH). Therefore ...
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No products found
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Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... IP of total SUMOylated protein fractions used either SUMO-1 or SUMO-2/3 Ab included with the Signal Seeker SUMOylation 1 or 2/3 Detection Kit (Cytoskeleton) and IPs were carried out according to the manufacturer instructions ...
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No products found
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Stephen B. McHugh, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Sections were then incubated with primary antibodies diluted in 3% NDS blocking solution and incubated at 4 °C for 72 hours (GFP anti-chicken, 1:1,000, Aves Labs, catalog no ...
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No products found
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Dimitrios C Arhontoulis, et al.,
bioRxiv - Bioengineering 2022
Quote:
Drugs were added to organoids concurrently with IL-1 β on Days 2 and 4 upon media replacement: Tocilizumab (Cat: A1447-200, Lot: 6G02A14470, Biovision), Human IL-1RA (Cat:4263-100 ...
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No products found
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Surya Prakash Rao Batta, et al.,
bioRxiv - Cell Biology 2022
Quote:
HUVECs (passage 2 to 6; PromoCell) were routinely cultured in EBM media supplemented with the provided growth factors kit (Promocell) ...
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No products found
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S. Bayraktar, et al.,
bioRxiv - Cell Biology 2019
Quote:
... 2×104 cells/ml were seeded into 4-well or 8-well glass-bottomed dishes (Sarstedt) and incubated for 24 h ...
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No products found
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Néstor Sampedro Vallina, et al.,
bioRxiv - Bioengineering 2023
Quote:
... (5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl)-4H-imidazol-4-one (DFHBI-1T) was purchased from Lucerna Technologies ...
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No products found
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Danielle M Paul, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Kinesore (3,5-dibromo-N′-[2,5-dimethyl-1-(3-nitrophenyl)-1H-pyrrol-3-yl]methylene}-4-hydroxybenzohydrazide) was obtained from Chembridge Corporation (Cat ...
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No products found
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Sherine E. Thomas, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Wizard 3&4 (Molecular Dimensions), JCSG +Suite (Molecular Dimensions) ...
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No products found
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Ami Vadgama, et al.,
bioRxiv - Immunology 2023
Quote:
... 3-100μM protease-activated receptor 4 agonist (PAR-4; Cambridge Biosciences); 0.03-3μM collagen-related peptide (CRP-XL ...
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No products found
because this supplier's products are not listed.
Gina Partipilo, et al.,
bioRxiv - Bioengineering 2021
Quote:
... 2-(4-((bis((1-(tert-butyl)-1H-1,2,3-triazol-4-yl)methyl)amino)methyl)-1H-1,2,3-triazol-1-yl)acetic acid (BTTAA, Click Chemistry Tools > 95%), Click-IT™ Fucose Alkyne (Invitrogen) ...
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No products found
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Yinan Hu, et al.,
bioRxiv - Developmental Biology 2019
Quote:
The fluorescent mitochondrial stain 4-(4-Diethylaminostyryl)-1-methylpyridinium iodide (DiASP, AdipoGen) was used to visualize neuromasts (Collazo et al. ...
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No products found
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Gabriela Souza Barbosa, et al.,
bioRxiv - Physiology 2023
Quote:
Male and female C57Bl/6 mice (6-8 weeks of age) were divided into 4 groups and fed either a normal diet (ND; Research Diets, D12328 ...
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No products found
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Sabina Gherman, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and/or subdural strip/grid electrodes (2- or 3-mm platinum disks with 4- or 10-mm center to center distance; PMT corporation, Chanassen, MN). The placement of the electrodes was determined exclusively based on clinical considerations ...
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No products found
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Avi I. Flamholz, et al.,
bioRxiv - Microbiology 2022
Quote:
... 4 and 6 were conducted in 96-well plates in a gas-controlled plate reader (Tecan Spark). For the data in Figure 3 ...
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No products found
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Calvin Yeager, et al.,
bioRxiv - Biochemistry 2022
Quote:
... 500 mM NaCl] overnight at 4 °C by using a 6-8,000 MWCO dialysis membrane (Spectrum Laboratories). The dialyzed 2C protein was centrifuged at 75,000 x g at 4 °C for 30 min to isolate soluble protein and concentrated using a Vivaspin Turbo 15 (Sartorius ...
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No products found
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Garrett D. Sheehan, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Slides were next gently rocked in a humidity chamber overnight at 4° C with Blocking Buffer containing either Mouse anti-Magi-2 (1:250, Abnova), Guinea Pig anti-PKCγ (1:500 ...
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No products found
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Sandra Olenic, Lim Heo, Michael Feig, Lee Kroos,
bioRxiv - Biochemistry 2021
Quote:
... The lipophilic dye FM 4-64 (1 μg/mL) (AAT Bioquest) was used to stain membranes ...
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No products found
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Linlin Bao, et al.,
bioRxiv - Microbiology 2020
Quote:
... paraffin dehydrated sections (3-4 μm in thickness) were treated with an antigen retrieval kit (Boster, AR0022) for 1 min at 37°C and quenched for endogenous peroxidases in 3% H2O2 in methanol for 10 min ...
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No products found
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Anna Cutrina-Pons, et al.,
bioRxiv - Immunology 2022
Quote:
... cultures were stimulated with IL-4 (200 IU/ml; R&D Europe Systems Ltd) and anti-CD40 antibody (0.5 μg/ml; G28.5; American Type Culture Collection). To investigate the role of PI3K p110δ signalling in human IgE production ...
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No products found
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Charlotte Avet, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... cells (4 x 105 cells/dish) were seeded into 35-mm poly-d-lysine- coated glass-bottom culture dishes (Mattek Corporation; Ashland, MA, USA) in 2 ml of fresh medium and incubated at 37°C in 5% CO2 ...