Laura Ohl, et al.,bioRxiv - Molecular Biology 2023
Quote:
... Tissue was mixed with 1 mL 40:40:20 methanol:acetonitrile:water (extraction solvent) and homogenized with a benchtop lyser (SciLogex OS20-S) at 1600 mhZ for approximately 15 sec ...
Adithya Ramesh, et al.,bioRxiv - Synthetic Biology 2022
Quote:
... and 4 were cloned into the AarI digested pCas9yl-GW vector using the Gibson Assembly HiFi HC 1-step Master Mix (SGI-DNA). Library 2 was digested with AarI and cloned into pCas9yl-GW digested with AarI using Golden Gate assembly with T4 DNA ligase (NEB).
Kevin M. Tharp, et al.,bioRxiv - Cell Biology 2020
Quote:
... 1 μL of sample was separated over a 25 cm column packed with 1.9 μm Reprosil C18 particles (Dr. Maisch HPLC GmbH) by a nanoElute HPLC (Bruker) ...