-
No products found
because this supplier's products are not listed.
Chetan Kumar Arya, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and Wizard Classic 1 and 2 (Rigaku Japan). Crystals of DMFase were obtained by mixing 200 nl of protein in buffer A with equal volumes of precipitant ...
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No products found
because this supplier's products are not listed.
Yan Zhang, et al.,
bioRxiv - Neuroscience 2021
Quote:
... at ∼1×105 cells per well in 100 µL of a 1:2 mixture of NbActiv4 (BrainBits) and plating medium (28 mM glucose ...
-
No products found
Emily E. Bonacquisti, et al.,
bioRxiv - Bioengineering 2021
Quote:
... The sEVs were then incubated with a 2:1 mass ratio of TO-3 or TO-1 (ABM Technologies) for 30 min at room temperature ...
-
No products found
because this supplier's products are not listed.
Iliodora V. Pop, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Mice (1-2 months old) were injected with 4% (w/v) FG solution in saline (Fluorochrome). Mice were anesthetized with isoflurane and the area above and around the cerebellar region was prepared for surgery ...
-
No products found
because this supplier's products are not listed.
Nathaniel L. Hepowit, et al.,
bioRxiv - Cell Biology 2022
Quote:
... anti-ubiquitin (1:10,000; LifeSensors; Mab; clone VU-1), anti-K63 (1:1,000 ...
-
No products found
because this supplier's products are not listed.
Laurent Jacob, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Serial cross sections (5□µm thick) were immunostained with rabbit anti-mouse LYVE1 (1:100) polyclonal antibody (11-034, AngioBio Co). DAB (3,3′ -Diaminobenzidine ...
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No products found
because this supplier's products are not listed.
Nauman Malik, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 5% bovine serum albumin and 0.05% sodium-Azide) containing either MOAB-2 (1:1000, Cat# M-1586-100, Biosensis) for the detection of Aβ or AT-8 (1:1000 ...
-
No products found
because this supplier's products are not listed.
Siranush Babakhanova, et al.,
bioRxiv - Molecular Biology 2021
Quote:
Two-photon spectrum and cross sections of TagRFP658 were measured in PBS buffer at concentrations ∼1–5·10−5 M in 1 mm glass spectroscopy cuvettes (Starna cells) using an MOM two-photon fluorescent microscope (Sutter Instrument ...
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No products found
because this supplier's products are not listed.
Miguel Alejandro Lopez-Ramirez, et al.,
bioRxiv - Biochemistry 2019
Quote:
... and 2-amino-N-(1-phenylethyl)benzamide (Enamine).
-
No products found
because this supplier's products are not listed.
Ruth I. Connor, et al.,
bioRxiv - Immunology 2023
Quote:
... Omicron BA.1 or Omicron BA.2 (Immune Technology) diluted to 5 μg/ml in 1X PBS and incubated overnight at 4 °C ...
-
No products found
because this supplier's products are not listed.
Vitor Mendes, et al.,
bioRxiv - Biochemistry 2020
Quote:
... was mixed in 1:1 and 1:2 (protein to reservoir) ratio with well solution using a mosquito robot (TTP labtech). Initial conditions were obtained in the Classics lite crystallization screen (Qiagen) ...
-
No products found
because this supplier's products are not listed.
Amy Cheung, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Membranes were incubated with primary antibodies prepared in 1% skim milk and 1% Bovine Serum Albumin in TBST for 2 hours (mouse anti-SERT 1:7000, MAb Technologies, ST51-2 ...
-
No products found
because this supplier's products are not listed.
Saurav Kumar, et al.,
bioRxiv - Cell Biology 2023
Quote:
... pMD2.G and psPAX2 in 2:1:2 ratio with PolyJet transfection reagent (SignaGen Laboratories). Media were harvested two days later and added to recipient cells with 1 μg/ml polybrene (Sigma ...
-
No products found
because this supplier's products are not listed.
Allison R. Fusilier, et al.,
bioRxiv - Neuroscience 2021
Quote:
... BMAL1 (1:1000 in 5% BSA and TBST, Signalway Antibody, LLC, #21415,), GSK3β (3D10 ...
-
No products found
because this supplier's products are not listed.
Shijian Zhang, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... resuspended in 1×PBS to a final concentration of 5 mg of wet membrane per ml of 1×PBS and crosslinked with 5 mM BS3 (Proteochem), followed by solubilization with a solubilization buffer containing 100 mM (NH4)2SO4 ...
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magnetofection
difficult to transfect cells
Cat# KC30296,
PolyMag 100µL+PolyMag Neo100µL+ CombiMag 100µL+Magnetic Plate MF96000, USD $640.63/KIT
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Yuan-Chen Tsai, et al.,
bioRxiv - Neuroscience 2022
Quote:
... were mixed with 2 μl lipofectamine 2000 (Thermo Fischer Scientific) and 1 μl (1:10 diluted) magnetofection beads (CombiMag, OZ Bioscience). The plasmid-lipofectamine-magnetofection mix were incubated at room temperature for 15 min before adding to neurons ...
-
No products found
because this supplier's products are not listed.
Jie Su, Naoko Toyofuku, Takuro Nakagawa,
bioRxiv - Genomics 2021
Quote:
... and 1 g/l 5-fluoroorotic acid monohydrate (Apollo Scientific, Stockport, United Kingdom, PC4054). Yeast cells were grown at 30°C.
-
No products found
because this supplier's products are not listed.
Artem I. Fokin, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 5 µg/mL for the secondary) or 1:3000 diluted SiR-actin (Tebu-bio). Nuclei were counter stained with DAPI (Live Technologies) ...
-
No products found
because this supplier's products are not listed.
Caroline Murawski, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and 2 µm S1818 (Microchem, 5000 rpm, baking at 100°C for 1 min). Patterns were developed for 40 – 50 s in MF319 (Microchem) ...
-
No products found
because this supplier's products are not listed.
Ana L. Pereira, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... the coverslips were blocked for 1 h at RT with 2% BSA (NZYTech, MB04602), 2% FBS (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Zahra Mashhadi, et al.,
bioRxiv - Biochemistry 2024
Quote:
... 5 µl of injection mixture consist of 1 µg of recombinant nls-Cas9 (PNA Bio), 200 ng of purified sgRNA ...
-
No products found
because this supplier's products are not listed.
Timothy A. Troppoli, et al.,
bioRxiv - Neuroscience 2023
Quote:
Mice were deeply anesthetized with isoflurane (1-3%) and viral injections were performed using a 1 µl Hamilton syringe (Cat#87900, Point Style 2, Hamilton Company, Reno, NV, USA), targeting BF (AP +0.14 mm ...
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No products found
because this supplier's products are not listed.
Ekaterina Kropocheva, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... 5% glycerol) supplemented with 1 mM of PMSF and disrupted using Cell Disruptor CF (Constant Systems). The lysate was cleared by centrifugation ...
-
No products found
because this supplier's products are not listed.
Alyssa Huff, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 1.5 CaCl2, 30 D-glucose) equilibrated with carbogen (95% O2, 5% CO2) by a peristaltic pump (Dynamax RP-1, Rainin Instrument Co; Emeryville CA, USA). As previously published (figure 6a (Huff et al. ...
-
No products found
because this supplier's products are not listed.
Chen Sun, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... followed by gentle vortexing for 5 s and centrifugation for 1 min (Benchmark Scientific, MyFuge Mini centrifuge). After droplet breaking ...
-
No products found
because this supplier's products are not listed.
Drew T. Dunham, et al.,
bioRxiv - Microbiology 2022
Quote:
... 5 μg of RNA was ran on a 8% polyacrylamide urea gel (National Diagnostics SequaGel 1:4) in 1x TBE buffer (100mM Tris base ...
-
No products found
because this supplier's products are not listed.
Eric Vallabh Minikel, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Samples were injected (1 to 2 µg) onto a 75 um ID PicoFrit column (New Objective) packed to 20 cm with Reprosil-Pur C18 AQ 1.9 um media (Dr ...
-
No products found
because this supplier's products are not listed.
Bente K. Hansen, et al.,
bioRxiv - Cell Biology 2019
Quote:
... 1 mM DTT) before resuspended in buffer CT (5 mL) and loaded onto a butyl-ToyoPearl column (TOSOH) equilibrated in buffer C ...
-
No products found
because this supplier's products are not listed.
Scott P. Souza, et al.,
bioRxiv - Immunology 2021
Quote:
... and blocked with coating buffer containing with 1% BSA (w/v) and 2% goat serum (Omega Scientific) in PBS ...
-
No products found
because this supplier's products are not listed.
Christopher J. DiRusso, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... A 0.4 M solution of 2-(7-aza-1Hbenzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HATU) (Chem-Impex International) was prepared in DMF and 2.5 ml was used to dissolve 1 mmol of each Nα Fmoc-protected aa (Chem-Impex International) ...
-
No products found
because this supplier's products are not listed.
Carine Rey, et al.,
bioRxiv - Genomics 2022
Quote:
... belari bub-1 (gene mbelari.g12204) (1/10000 Covalab). Two peptides C-ALNASKEKPEEQLD-coNH2 and C-SPIVEDQDHENSTNG-coNH2 were injected in rabbits and the purified serum obtained at day 74 was used for staining.
-
No products found
because this supplier's products are not listed.
David M. Walter, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... at 15 mg kg−1 for a dose of 2 mg/kg/day or IACS-10759 (MedChem Express) at 37.5 mg kg−1 for a dose of 5 mg/kg/day by Research Diets. Mice were placed on treatment diet 4 weeks prior to analysis ...
-
No products found
because this supplier's products are not listed.
Frank Hidalgo, et al.,
bioRxiv - Biophysics 2022
Quote:
... Peptides were desalted for 4 minutes on a trap column (1 mM ID x 2 cm, IDEX C-128) manually packed with POROS R2 reversed-phase resin (Thermo Scientific 1112906) ...
-
No products found
because this supplier's products are not listed.
Zachary M. Earley, et al.,
bioRxiv - Immunology 2022
Quote:
Whole tissue biopsies stored at -80 °C were thawed on ice and transferred to Starstedt tubes containing 350uL RLT Plus supplemented with 1% 2-mercaptoethanol and equal quantities of 1.0 mm and 0.5 mm zirconium oxide beads (Next Advance). Biopsies were bead beat 3 times for 1 min at a setting of 9 on a Bullet Blender 24 ...
-
No products found
because this supplier's products are not listed.
Shira Yanovsky-Dagan, et al.,
bioRxiv - Genetics 2019
Quote:
... and then 1–2 μg RNA was reverse-transcribed by random hexamer priming and Multi Scribe reverse transcriptase (ABI). Amplification was performed using the primers ...
-
No products found
because this supplier's products are not listed.
Pallab Pradhan, et al.,
bioRxiv - Immunology 2020
Quote:
... Anti-CD3/CD28 bead (Dynabeads)-activated PBMCs (MSC/PBMC at 1:2) from healthy volunteers (purchased from Zen-Bio) plated in 200 ul culture media (RPMI 1640 + 10% FBS + 1% PS+ 30 IU/mL IL2 ...
-
No products found
because this supplier's products are not listed.
Aarini Ghosh, et al.,
bioRxiv - Zoology 2023
Quote:
Scanning Electron microscope imaging of stridulatory teeth was done at 1–2 kV resolution using JSM-6100 (JEOL, Japan) in the Department of Sophisticated Analytical Instrumentation Facility ...
-
No products found
because this supplier's products are not listed.
Raissa R. Christoff, et al.,
bioRxiv - Neuroscience 2021
Quote:
... aliquots were centrifugated and washed trice in 0.1M PBS (1,500RPM, 5 min each) and then incubated with 1:200 mouse anti-SATB21:200 (GenWay 20-372-60065) in blocking solution (2μl BSA 5% ...
-
No products found
because this supplier's products are not listed.
Katy R. McCarron, et al.,
bioRxiv - Cell Biology 2023
Quote:
... anti-LC3 (1:200 WB, 1:200 IF, Nanotools; 5F10), anti-p62 (1:1,000 WB ...
-
No products found
because this supplier's products are not listed.
Antonella Scaglione, et al.,
bioRxiv - Immunology 2021
Quote:
... COVID-19 convalescent plasma was diluted (1:10) and incubated with recombinant SARS-CoV-2 full-length Spike (BPS Bioscience) for 1 h at 37 °C prior to adding to an hACE2 pre-coated ELISA plates ...
-
No products found
because this supplier's products are not listed.
Hua He, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... or NKX2-1 antibody (Seven Hills Bioreagents, WRAB-1231, 1:1000) followed by incubation with TrueBlot HRP-Conjugated secondary antibody (Rockland Immunochemicals Inc ...
-
No products found
because this supplier's products are not listed.
Mohanraj Gopalswamy, et al.,
bioRxiv - Biophysics 2021
Quote:
The data was analyzed and the Ktet (Eq. 1) or EC50 (Eq. 2) calculated using the MO affinity analysis software (NanoTemper, Germany), considering a 1:1 model.
-
No products found
because this supplier's products are not listed.
Matthew D. Lauver, et al.,
bioRxiv - Immunology 2022
Quote:
... Virus dilutions were combined 1:1 with 0.45% sheep erythrocytes (Innovative Research) and incubated overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Jiacheng Gu, et al.,
bioRxiv - Genomics 2023
Quote:
... 2 μl of 0.5 mM 5’ amine-modified DNA probes (Integrated DNA technologies) is mixed with 1 μl of 10 mM ATTO488-NHS ester (ATTO-TEC AD 488-31), ATTO 643-NHS ester (ATTO-TEC AD 643-31 ...
-
No products found
because this supplier's products are not listed.
Sumeda Nandadasa, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Each sample was labeled with 2 units of each channel by adding 1 sample volume of dimethyl sulfoxide (DMSO) to each iTRAQ label (Sciex, catalog no.4390812) before adding the total solution volume to each sample ...
-
No products found
because this supplier's products are not listed.
Jianing Song, et al.,
bioRxiv - Biochemistry 2020
Quote:
... 1 μM ubiquitin aldehyde (Boston Biochem), 10 mM MgCl2 ...
-
No products found
because this supplier's products are not listed.
Beibei Wu, et al.,
bioRxiv - Microbiology 2021
Quote:
... that for detection of interferon alpha and beta receptor 1 (IFNAR-1) was purchased from Leinco Technologies, Inc ...
-
No products found
because this supplier's products are not listed.
F. Guo, et al.,
bioRxiv - Bioengineering 2024
Quote:
... the cell-laden constructs were infused with 20 μL of a 2 m mL-1 collagen gel and fixed into a 0.7x0.7 cm square chamber designed from Grace Bio-labs SecureSeal adhesive sheet ...
-
No products found
because this supplier's products are not listed.
Haylie R. Helms, et al.,
bioRxiv - Bioengineering 2024
Quote:
GFP+ HUVEC and RFP+ MDA-MB-231 were printed onto a collagen type 1 substrate and in cultured in a 1:1 ratio of HUVEC media (VascuLife VEGF, Lifeline Cell Technology) and MDA-MB-231 media (DMEM + 10% FBS + 1% penicillin-streptomycin) ...
-
No products found
Catalina Pereira, et al.,
bioRxiv - Genetics 2021
Quote:
... using a 1:1 ratio of WesternBright ECL Luminol/enhancer solution to WesternBright Peroxide Chemiluminescent peroxide solution (Advansta). Antibody information is provided in key resources table.